################################## # NO CUT ENZYMES ( 164 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspHI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII KasI KpnI KspI MamI MluI MluNI MroI MscI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PleI PmaCI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AciI 492 CCGC CCGC(-3/-1) AflIII 285 ACATGT A^C[GA][CT]GT AluI 748 AGCT AG^CT AseI 111 ATTAAT AT^TAAT AsnI 111 ATTAAT AT^TAAT AspI 871 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC BbvCI 183 CCTCAGC CCTCAGC(-5/-2) BpmI 202 CTGGAG CTGGAG(16/14) BsaAI 763 TACGTG [CT]AC^GT[GA] BsmAI 634 GTCTC GTCTC(1/5) BspLU11I 285 ACATGT A^CATGT DdeI 184 CTCAG C^T[ACGT]AG DsaI 314 CCATGG C^C[GA][CT]GG EaeI 684 TGGCCG [CT]^GGCC[GA] EcoRI 28 GAATTC G^AATTC HapII 687 CCGG C^CGG HinfI 843 GAATC G^A[ACGT]TC HpaI 828 GTTAAC GTT^AAC HpaII 687 CCGG C^CGG HphI 722 TCACC GGTGA(8/7) MfeI 535 CAATTG C^AATTG MspA1I 747 CAGCTG C[AC]G^C[GT]G MspI 687 CCGG C^CGG MunI 535 CAATTG C^AATTG MwoI 740 GCTATGGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NcoI 314 CCATGG C^CATGG NspI 285 ACATGT [GA]CATG^[CT] PflFI 871 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 52 GGGTCCC [GA]G^G[AT]CC[CT] PshBI 111 ATTAAT AT^TAAT PstI 160 CTGCAG CTGCA^G PvuII 747 CAGCTG CAG^CTG SapI 252 GCTCTTC GCTCTTC(1/4) SfcI 160 CTGCAG C^T[GA][CT]AG SmlI 664 CTCAAG C^T[CT][GA]AG TfiI 843 GAATC G^A[AT]TC TspRI 24 CACTG CA[GC]TG[ACGT][ACGT]^ Tth111I 871 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AcsI 28 GAATTC [GA]^AATT[CT] 501 AAATTC AlwNI 402 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG 677 CAGCCTCTG ApoI 28 GAATTC [GA]^AATT[CT] 501 AAATTC Asp700I 693 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 843 GAATCCTTTC BfaI 17 CTAG C^TAG 40 CTAG BstYI 206 AGATCC [GA]^GATC[CT] 668 AGATCC DraII 52 GGGTCCC [GA]G^G[ACGT]CC[CT] 884 AGGCCCT EcoO109I 52 GGGTCCC [GA]G^G[ACGT]CC[CT] 884 AGGCCCT EcoT14I 57 CCAAGG C^C[AT][AT]GG 314 CCATGG HincII 828 GTTAAC GT[CT]^[GA]AC 914 GTCAAC HindII 828 GTTAAC GT[CT]^[GA]AC 914 GTCAAC MaeI 17 CTAG C^TAG 40 CTAG MaeII 156 ACGT A^CGT 764 ACGT MflI 206 AGATCC [GA]^GATC[CT] 668 AGATCC SfaNI 237 GATGC GCATC(5/9) 263 GATGC StyI 57 CCAAGG C^C[AT][AT]GG 314 CCATGG XhoII 206 AGATCC [GA]^GATC[CT] 668 AGATCC XmnI 693 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 843 GAATCCTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AfaI AlwI AvaII BbvI BsaJI BsiYI BslI BsmFI BssKI BstF5I BstNI Cfr13I DpnII EarI EcoRII Fnu4HI FokI HaeIII ItaI Ksp632I MaeIII MboI MboII MnlI MseI MslI MvaI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI Tru9I TseI Tsp45I Tsp509I