(Circular) (Six-base) MAPSORT of: pFN21AE5911.seq Check: 9937 from: 1 to: 5635 pFN21AE5911 5635 bp With 182 enzymes: SgfI * December 8, 2014 13:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3445 278 Size: 53 83 186 936 1909 2468 Fragments arranged by size: 2468 1909 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2978 1937 Size: 1041 4594 Acc65I G'GTAC_C Cuts at: 2956 2956 Size: 5635 AclI AA'CG_TT Cuts at: 1563 3762 4135 1563 Size: 2199 373 3063 Fragments arranged by size: 3063 2199 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3698 5082 5350 1632 Size: 2066 1384 268 1917 Fragments arranged by size: 2066 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5438 AflIII A'CryG_T Cuts at: 1203 2290 4534 5511 1203 Size: 1087 2244 977 1327 Fragments arranged by size: 2244 1327 1087 977 AgeI A'CCGG_T Cuts at: 4466 4466 Size: 5635 AhdI GACnn_n'nnGTC Cuts at: 4364 4364 Size: 5635 AleI CACnn'nnGTG Cuts at: 1166 2022 1166 Size: 856 4779 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5635 AlwNI CAG_nnn'CTG Cuts at: 2412 2687 3378 4950 2412 Size: 275 691 1572 3097 Fragments arranged by size: 3097 1572 691 275 ApaLI G'TGCA_C Cuts at: 3692 4848 3692 Size: 1156 4479 ApoI r'AATT_y Cuts at: 1493 2033 2506 2944 3170 3322 5431 1493 Size: 540 473 438 226 152 2109 1697 Fragments arranged by size: 2109 1697 540 473 438 226 152 AseI AT'TA_AT Cuts at: 160 1032 2117 4189 160 Size: 872 1085 2072 1606 Fragments arranged by size: 2072 1606 1085 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5635 AvaI C'yCGr_G Cuts at: 1870 1943 2960 1870 Size: 73 1017 4545 Fragments arranged by size: 4545 1017 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2392 2425 2392 Size: 33 5602 BamHI G'GATC_C Cuts at: 2965 3332 2965 Size: 367 5268 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2956 4411 618 Size: 325 217 627 1169 1455 1842 Fragments arranged by size: 1842 1455 1169 627 325 217 BanII G_rGCy'C Cuts at: 1391 2954 1391 Size: 1563 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4664 BbvCI CC'TCA_GC Cuts at: 2189 2189 Size: 5635 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3826 3860 1903 Size: 34 1889 34 3678 Fragments arranged by size: 3678 1889 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3527 4737 3527 Size: 1210 4425 BclI T'GATC_A Cuts at: 1548 1548 Size: 5635 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4246 136 Size: 107 122 71 631 605 2574 1525 Fragments arranged by size: 2574 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5630 5630 Size: 5635 BlpI GC'TnA_GC Cuts at: 3052 3052 Size: 5635 Bme1580I G_kGCm'C Cuts at: 1790 3696 4852 1790 Size: 1906 1156 2573 Fragments arranged by size: 2573 1906 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5635 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3366 4324 442 Size: 416 53 83 399 24 1949 958 1753 Fragments arranged by size: 1949 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5635 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2228 4295 1239 Size: 989 2067 2579 Fragments arranged by size: 2579 2067 989 Bpu10I CC'TnA_GC Cuts at: 2189 2189 Size: 5635 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2654 3760 4625 4923 2654 Size: 1106 865 298 3366 Fragments arranged by size: 3366 1106 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4298 882 Size: 617 2799 2219 Fragments arranged by size: 2799 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2770 3426 493 Size: 711 1566 656 2702 Fragments arranged by size: 2702 1566 711 656 BsaBI GATnn'nnATC Cuts at: 5629 5629 Size: 5635 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3442 3824 4459 275 Size: 53 83 186 936 1909 382 635 1451 Fragments arranged by size: 1909 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4068 4466 4740 4887 5298 4068 Size: 398 274 147 411 4405 Fragments arranged by size: 4405 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2642 2672 1178 Size: 30 165 30 1239 30 4141 Fragments arranged by size: 4141 1239 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5635 BseYI C'CCAG_C Cuts at: 4838 5334 4838 Size: 496 5139 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5062 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3085 3846 4874 1540 Size: 275 186 1084 761 1028 2301 Fragments arranged by size: 2301 1084 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3696 3781 4852 3696 Size: 85 1071 4479 Fragments arranged by size: 4479 1071 85 BsmI GAATG_Cn' Cuts at: 3146 3239 3146 Size: 93 5542 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2954 3696 3781 4852 1391 Size: 399 1164 742 85 1071 2174 Fragments arranged by size: 2174 1164 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5298 5298 Size: 5635 BspHI T'CATG_A Cuts at: 3524 5254 3524 Size: 1730 3905 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2990 844 Size: 2146 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3522 1394 Size: 429 1699 3507 Fragments arranged by size: 3507 1699 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4130 4304 65 Size: 1183 2882 174 1396 Fragments arranged by size: 2882 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4279 4466 1700 Size: 110 2469 187 2869 Fragments arranged by size: 2869 2469 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5635 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5635 BssSI C'ACGA_G Cuts at: 3695 4707 3695 Size: 1012 4623 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5635 BstBI TT'CG_AA Cuts at: 4439 4439 Size: 5635 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5635 BstYI r'GATC_y Cuts at: 1972 2211 2673 2965 3332 3717 3734 5186 5272 Size: 239 462 292 367 385 17 1452 86 Cuts at: 5272 5284 5630 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 462 385 367 346 292 239 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2319 513 Size: 552 1254 3829 Fragments arranged by size: 3829 1254 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4896 Fragments arranged by size: 4896 683 56 BtsI GCAGTG_nn' Cuts at: 3151 3945 3965 3151 Size: 794 20 4821 Fragments arranged by size: 4821 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5635 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5600 DraI TTT'AAA Cuts at: 2939 2939 Size: 5635 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4642 809 Size: 507 3326 1802 Fragments arranged by size: 3326 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2689 3082 3971 Size: 54 1425 50 275 18 859 393 889 Cuts at: 3971 5600 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 859 393 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3082 1537 Size: 275 1270 4090 Fragments arranged by size: 4090 1270 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2264 2574 2871 3565 1353 Size: 911 310 297 694 3423 Fragments arranged by size: 3423 911 694 310 297 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4222 4596 4742 387 Size: 1064 138 2633 374 146 1280 Fragments arranged by size: 2633 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2228 3698 4295 5082 5350 1239 Size: 393 596 1470 597 787 268 1524 Fragments arranged by size: 1524 1470 787 597 596 393 268 EcoO109I rG'GnC_Cy Cuts at: 2058 2890 2058 Size: 832 4803 EcoRI G'AATT_C Cuts at: 2033 2944 3322 2033 Size: 911 378 4346 Fragments arranged by size: 4346 911 378 FspI TGC'GCA Cuts at: 4141 4141 Size: 5635 HaeII r_GCGC'y Cuts at: 1678 4782 1678 Size: 3104 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2003 2035 4276 4308 4350 Size: 32 135 32 431 32 2241 32 42 Cuts at: 4350 4382 1373 Size: 32 2626 Fragments arranged by size: 2626 2241 431 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2835 2921 2979 3225 669 Size: 988 281 897 86 58 246 3079 Fragments arranged by size: 3079 988 897 281 246 86 58 HindIII A'AGCT_T Cuts at: 748 748 Size: 5635 HpaI GTT'AAC Cuts at: 2835 3225 2835 Size: 390 5245 KpnI G_GTAC'C Cuts at: 2960 2960 Size: 5635 MfeI C'AATT_G Cuts at: 2540 3234 2540 Size: 694 4941 MluI A'CGCG_T Cuts at: 5511 5511 Size: 5635 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4749 4933 1305 Size: 3444 184 2007 Fragments arranged by size: 3444 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3813 Fragments arranged by size: 3813 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2022 2138 2396 3593 3952 4111 518 Size: 648 856 116 258 1197 359 159 2042 Fragments arranged by size: 2042 1197 856 648 359 258 159 116 MspA1I CmG'CkG Cuts at: 1606 2754 3051 3728 4876 5121 1606 Size: 1148 297 677 1148 245 2120 Fragments arranged by size: 2120 1148 1148 677 297 245 NcoI C'CATG_G Cuts at: 513 1065 2319 513 Size: 552 1254 3829 Fragments arranged by size: 3829 1254 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5635 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5635 NotI GC'GGCC_GC Cuts at: 3082 3082 Size: 5635 NspI r_CATG'y Cuts at: 1132 1229 2294 2993 4538 1132 Size: 97 1065 699 1545 2229 Fragments arranged by size: 2229 1545 1065 699 97 PasI CC'CwG_GG Cuts at: 1348 2054 1348 Size: 706 4929 PciI A'CATG_T Cuts at: 2290 4534 2290 Size: 2244 3391 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5635 PmeI GTTT'AAAC Cuts at: 2939 2939 Size: 5635 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3704 3736 5217 5249 3704 Size: 32 1481 32 4090 Fragments arranged by size: 4090 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2058 2058 Size: 5635 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5635 PsiI TTA'TAA Cuts at: 3205 3205 Size: 5635 PstI C_TGCA'G Cuts at: 830 2169 2987 830 Size: 1339 818 3478 Fragments arranged by size: 3478 1339 818 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5635 PvuII CAG'CTG Cuts at: 2754 2754 Size: 5635 SalI G'TCGA_C Cuts at: 1936 2977 1936 Size: 1041 4594 SanDI GG'GwC_CC Cuts at: 2058 2058 Size: 5635 SapI GCTCTTCn'nnn_ Cuts at: 2264 2264 Size: 5635 SbfI CC_TGCA'GG Cuts at: 2987 2987 Size: 5635 ScaI AGT'ACT Cuts at: 3883 5595 3883 Size: 1712 3923 SfcI C'TryA_G Cuts at: 826 1045 2165 2983 4118 4799 4990 826 Size: 219 1120 818 1135 681 191 1471 Fragments arranged by size: 1471 1135 1120 818 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5635 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5635 SmaI CCC'GGG Cuts at: 1872 2962 1872 Size: 1090 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2669 3739 4640 4902 820 Size: 197 926 726 1070 901 262 1553 Fragments arranged by size: 1553 1070 926 901 726 262 197 SnaBI TAC'GTA Cuts at: 493 3426 493 Size: 2933 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5635 SphI G_CATG'C Cuts at: 1132 2993 1132 Size: 1861 3774 SspI AAT'ATT Cuts at: 5 52 3559 5 Size: 47 3507 2081 Fragments arranged by size: 3507 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5635 StyI C'CwwG_G Cuts at: 513 1065 2062 2319 3074 513 Size: 552 997 257 755 3074 Fragments arranged by size: 3074 997 755 552 257 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2410 2428 2547 3662 3679 3832 4017 Size: 1255 830 18 119 1115 17 153 185 Cuts at: 4017 4462 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1115 830 445 185 153 119 18 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2799 3881 5593 Size: 274 80 33 51 1443 822 1082 1712 Cuts at: 5593 96 Size: 138 Fragments arranged by size: 1712 1443 1082 822 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5595 5627 5595 Size: 32 5603 Tth111I GACn'n_nGTC Cuts at: 1316 2879 1316 Size: 1563 4072 XbaI T'CTAG_A Cuts at: 2971 2971 Size: 5635 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5635 XmaI C'CCGG_G Cuts at: 1870 2960 1870 Size: 1090 4545 XmnI GAAnn'nnTTC Cuts at: 2702 2852 3764 2702 Size: 150 912 4573 Fragments arranged by size: 4573 912 150 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3443 276 Size: 53 83 186 936 1909 2468 Fragments arranged by size: 2468 1909 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI XcmI