Restriction map for
pFN21AE6384
(Circular) (Six-base) MAPSORT of: pFN21AE6384.seq Check: 3229 from: 1 to: 5698
pFN21AE6384 5698 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2416 2416
Size: 5698
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3508 278
Size: 53 83 186 936 1972 2468
Fragments arranged by size:
2468 1972 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3041 1937
Size: 1104 4594
Acc65I G'GTAC_C
Cuts at: 3019 3019
Size: 5698
AclI AA'CG_TT
Cuts at: 1563 2997 3825 4198 1563
Size: 1434 828 373 3063
Fragments arranged by size:
3063 1434 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3761 5145 5413 1632
Size: 2129 1384 268 1917
Fragments arranged by size:
2129 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5501
AflIII A'CryG_T
Cuts at: 1203 2757 4597 5574 1203
Size: 1554 1840 977 1327
Fragments arranged by size:
1840 1554 1327 977
AgeI A'CCGG_T
Cuts at: 4529 4529
Size: 5698
AhdI GACnn_n'nnGTC
Cuts at: 4427 4427
Size: 5698
AleI CACnn'nnGTG
Cuts at: 1166 2939 1166
Size: 1773 3925
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2062 1184
Size: 878 4820
AlwNI CAG_nnn'CTG
Cuts at: 3441 5013 3441
Size: 1572 4126
ApaLI G'TGCA_C
Cuts at: 3755 4911 3755
Size: 1156 4542
ApoI r'AATT_y
Cuts at: 1493 2688 2834 3007 3233 3385 5494 1493
Size: 1195 146 173 226 152 2109 1697
Fragments arranged by size:
2109 1697 1195 226 173 152 146
AseI AT'TA_AT
Cuts at: 160 1032 4252 160
Size: 872 3220 1606
Fragments arranged by size:
3220 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5698
AvaI C'yCGr_G
Cuts at: 1870 1943 3023 1870
Size: 73 1080 4545
Fragments arranged by size:
4545 1080 73
AvrII C'CTAG_G
Cuts at: 2953 2953
Size: 5698
BamHI G'GATC_C
Cuts at: 3028 3395 3028
Size: 367 5331
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2900 3019 4474 618
Size: 325 217 627 1113 119 1455 1842
Fragments arranged by size:
1842 1455 1113 627 325 217 119
BanII G_rGCy'C
Cuts at: 1391 2073 3017 1391
Size: 682 944 4072
Fragments arranged by size:
4072 944 682
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2620 2905 928
Size: 971 721 285 3721
Fragments arranged by size:
3721 971 721 285
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3889 3923 1903
Size: 34 50 34 1868 34 3678
Fragments arranged by size:
3678 1868 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3590 4800 3590
Size: 1210 4488
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5698
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4309 136
Size: 107 122 71 631 605 2637 1525
Fragments arranged by size:
2637 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5693 5693
Size: 5698
BlpI GC'TnA_GC
Cuts at: 3115 3115
Size: 5698
Bme1580I G_kGCm'C
Cuts at: 1790 2903 3759 4915 1790
Size: 1113 856 1156 2573
Fragments arranged by size:
2573 1156 1113 856
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5698
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2431 3429 4387
Size: 416 53 83 399 24 1014 998 958
Cuts at: 4387 442
Size: 1753
Fragments arranged by size:
1753 1014 998 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5698
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4358 1239
Size: 3119 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2467 3823 4688 4986 2467
Size: 1356 865 298 3179
Fragments arranged by size:
3179 1356 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4361 882
Size: 617 2862 2219
Fragments arranged by size:
2862 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2758 3489 493
Size: 711 1554 731 2702
Fragments arranged by size:
2702 1554 731 711
BsaBI GATnn'nnATC
Cuts at: 5692 5692
Size: 5698
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3505 3887 4522 275
Size: 53 83 186 936 1972 382 635 1451
Fragments arranged by size:
1972 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2297 4131 4529 4803 4950 5361 2297
Size: 1834 398 274 147 411 2634
Fragments arranged by size:
2634 1834 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2576 2606 1178
Size: 30 165 30 1173 30 4270
Fragments arranged by size:
4270 1173 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2723 1186
Size: 1537 4161
BseYI C'CCAG_C
Cuts at: 2896 4901 5397 2896
Size: 2005 496 3197
Fragments arranged by size:
3197 2005 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5125
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3148 3909 4937 1540
Size: 275 186 1147 761 1028 2301
Fragments arranged by size:
2301 1147 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2073 3759 3844 4915 2073
Size: 1686 85 1071 2856
Fragments arranged by size:
2856 1686 1071 85
BsmI GAATG_Cn'
Cuts at: 2798 3209 3302 2798
Size: 411 93 5194
Fragments arranged by size:
5194 411 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2073 2903 3017 3759 3844 4915 1391
Size: 399 283 830 114 742 85 1071 2174
Fragments arranged by size:
2174 1071 830 742 399 283 114 85
BspEI T'CCGG_A
Cuts at: 5361 5361
Size: 5698
BspHI T'CATG_A
Cuts at: 2736 3587 5317 2736
Size: 851 1730 3117
Fragments arranged by size:
3117 1730 851
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2416 3053 844
Size: 1572 637 3489
Fragments arranged by size:
3489 1572 637
BsrBI CCG'CTC
Cuts at: 1394 1823 3585 1394
Size: 429 1762 3507
Fragments arranged by size:
3507 1762 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2265 2373 4193 4367 65
Size: 1183 1017 108 1820 174 1396
Fragments arranged by size:
1820 1396 1183 1017 174 108
BsrFI r'CCGG_y
Cuts at: 1700 1810 4342 4529 1700
Size: 110 2532 187 2869
Fragments arranged by size:
2869 2532 187 110
BsrGI T'GTAC_A
Cuts at: 96 2931 96
Size: 2835 2863
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5698
BssSI C'ACGA_G
Cuts at: 3758 4770 3758
Size: 1012 4686
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5698
BstBI TT'CG_AA
Cuts at: 4502 4502
Size: 5698
BstEII G'GTnAC_C
Cuts at: 1184 2212 1184
Size: 1028 4670
BstXI CCAn_nnnn'nTGG
Cuts at: 2380 2380
Size: 5698
BstYI r'GATC_y
Cuts at: 1972 2294 3028 3395 3780 3797 5249 5335 5347
Size: 322 734 367 385 17 1452 86 12
Cuts at: 5347 5693 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 734 385 367 346 322 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2787 513
Size: 552 1722 3424
Fragments arranged by size:
3424 1722 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4959
Fragments arranged by size:
4959 683 56
BtsI GCAGTG_nn'
Cuts at: 3214 4008 4028 3214
Size: 794 20 4884
Fragments arranged by size:
4884 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5698
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5663
DraI TTT'AAA
Cuts at: 3002 3002
Size: 5698
DraIII CAC_nnn'GTG
Cuts at: 2886 2886
Size: 5698
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2437 4705 809
Size: 507 1121 2268 1802
Fragments arranged by size:
2268 1802 1121 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3145 4034 5663
Size: 54 1425 50 275 18 1315 889 1629
Cuts at: 5663 8
Size: 43
Fragments arranged by size:
1629 1425 1315 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3145 1537
Size: 275 1333 4090
Fragments arranged by size:
4090 1333 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2520 3628 1353
Size: 1167 1108 3423
Fragments arranged by size:
3423 1167 1108
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4285 4659 4805 387
Size: 1064 138 2696 374 146 1280
Fragments arranged by size:
2696 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2071 2071
Size: 5698
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3761 4358 5145 5413 1239
Size: 393 2129 597 787 268 1524
Fragments arranged by size:
2129 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2922 2922
Size: 5698
EcoRI G'AATT_C
Cuts at: 3007 3385 3007
Size: 378 5320
FspI TGC'GCA
Cuts at: 4204 4204
Size: 5698
HaeII r_GCGC'y
Cuts at: 1678 4845 1678
Size: 3167 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2497 2529 4339 4371 4413
Size: 32 135 32 925 32 1810 32 42
Cuts at: 4413 4445 1373
Size: 32 2626
Fragments arranged by size:
2626 1810 925 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3042 3288 669
Size: 988 281 1104 246 3079
Fragments arranged by size:
3079 1104 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5698
HpaI GTT'AAC
Cuts at: 3288 3288
Size: 5698
KpnI G_GTAC'C
Cuts at: 3023 3023
Size: 5698
MfeI C'AATT_G
Cuts at: 3297 3297
Size: 5698
MluI A'CGCG_T
Cuts at: 5574 5574
Size: 5698
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4812 4996 1305
Size: 3507 184 2007
Fragments arranged by size:
3507 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3876
Fragments arranged by size:
3876 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2210 2270 2411 2476 2702 2939 3656
Size: 648 1044 60 141 65 226 237 717
Cuts at: 3656 4015 4174 518
Size: 359 159 2042
Fragments arranged by size:
2042 1044 717 648 359 237 226 159
141 65 60
MspA1I CmG'CkG
Cuts at: 1606 3114 3791 4939 5184 1606
Size: 1508 677 1148 245 2120
Fragments arranged by size:
2120 1508 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5146
NdeI CA'TA_TG
Cuts at: 387 2283 387
Size: 1896 3802
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5698
NotI GC'GGCC_GC
Cuts at: 3145 3145
Size: 5698
NspI r_CATG'y
Cuts at: 1132 1229 3056 4601 1132
Size: 97 1827 1545 2229
Fragments arranged by size:
2229 1827 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5698
PciI A'CATG_T
Cuts at: 4597 4597
Size: 5698
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2096 2721 1108
Size: 988 625 4085
Fragments arranged by size:
4085 988 625
PfoI T'CCnGG_A
Cuts at: 2089 2089
Size: 5698
PmeI GTTT'AAAC
Cuts at: 3002 3002
Size: 5698
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3767 3799 5280 5312 3767
Size: 32 1481 32 4153
Fragments arranged by size:
4153 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2922 2922
Size: 5698
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5698
PsiI TTA'TAA
Cuts at: 2598 3268 2598
Size: 670 5028
PstI C_TGCA'G
Cuts at: 830 2051 3050 830
Size: 1221 999 3478
Fragments arranged by size:
3478 1221 999
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5698
SacI G_AGCT'C
Cuts at: 2073 2073
Size: 5698
SalI G'TCGA_C
Cuts at: 1936 3040 1936
Size: 1104 4594
SapI GCTCTTCn'nnn_
Cuts at: 2520 2520
Size: 5698
SbfI CC_TGCA'GG
Cuts at: 3050 3050
Size: 5698
ScaI AGT'ACT
Cuts at: 3946 5658 3946
Size: 1712 3986
SfcI C'TryA_G
Cuts at: 826 1045 2047 3046 4181 4862 5053 826
Size: 219 1002 999 1135 681 191 1471
Fragments arranged by size:
1471 1135 1002 999 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5698
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5698
SmaI CCC'GGG
Cuts at: 1872 3025 1872
Size: 1153 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2446 3802 4703 4965 820
Size: 197 926 503 1356 901 262 1553
Fragments arranged by size:
1553 1356 926 901 503 262 197
SnaBI TAC'GTA
Cuts at: 493 3489 493
Size: 2996 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5698
SphI G_CATG'C
Cuts at: 1132 3056 1132
Size: 1924 3774
SspI AAT'ATT
Cuts at: 5 52 3622 5
Size: 47 3570 2081
Fragments arranged by size:
3570 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5698
StyI C'CwwG_G
Cuts at: 513 1065 2016 2580 2953 3137 513
Size: 552 951 564 373 184 3074
Fragments arranged by size:
3074 951 564 552 373 184
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3725 3742 3895 4080 4525 325
Size: 1255 2145 17 153 185 445 1498
Fragments arranged by size:
2145 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2862 2931 3944
Size: 274 80 33 51 1443 885 69 1013
Cuts at: 3944 5656 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1013 885 274 138 80 69
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2169 2201 5658 5690 2169
Size: 32 3457 32 2177
Fragments arranged by size:
3457 2177 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5698
XbaI T'CTAG_A
Cuts at: 3034 3034
Size: 5698
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5698
XmaI C'CCGG_G
Cuts at: 1870 3023 1870
Size: 1153 4545
XmnI GAAnn'nnTTC
Cuts at: 2876 3827 2876
Size: 951 4747
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3506 276
Size: 53 83 186 936 1972 2468
Fragments arranged by size:
2468 1972 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII
SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI