Restriction map for
pFN21AE6075
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# NO CUT ENZYMES ( 143 enzymes) #
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AatII Acc65I AccII AclI AcyI
AflII AflIII AgeI AhdI AlwNI
AocI Aor51HI ApaI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BalI BamHI BbeI BbrPI
BciVI BclI BcnI BfrI BglI
BglII BlnI BpmI BsaAI BsaBI
BsaHI BsgI BsiEI BsiWI BsmBI
BspDI BspLU11I BspMI BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV FauI FbaI FseI
FspI HaeII HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeII MaeIII MamI MluI
MluNI MscI MseI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PstI PvuI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Tru9I
Tsp45I Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 72 enzymes) #
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AccI 520 GTCTAC GT^[AC][GT]AC
AccIII 872 TCCGGA T^CCGGA
AcsI 852 GAATTC [GA]^AATT[CT]
AfaI 597 GTAC GT^AC
Alw44I 792 GTGCAC G^TGCAC
ApaLI 792 GTGCAC G^TGCAC
ApoI 852 GAATTC [GA]^AATT[CT]
Asp700I 331 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 962 CCCGAG C^[CT]CG[GA]G
AvaII 785 GGTCC G^G[AT]CC
BanI 754 GGTGCC G^G[CT][GA]CC
BanII 46 GGGCTC G[GA]GC[CT]^C
BbvCI 909 GCTGAGG CCTCAGC(-5/-2)
BbvI 605 GCAGC GCAGC(8/12)
BcgI 482 CGATCTTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 319 CTAG C^TAG
BlpI 186 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 186 GCTCAGC GC^T[ACGT]AGC
BsaWI 872 TCCGGA [AT]^CCGG[AT]
BseAI 872 TCCGGA T^CCGGA
BsoBI 962 CCCGAG C^[CT]CG[GA]G
Bsp1407I 596 TGTACA T^GTACA
BspEI 872 TCCGGA T^CCGGA
BspHI 404 TCATGA T^CATGA
BsrGI 596 TGTACA T^GTACA
BssKI 782 CCAGG ^CC[ACGT]GG
BstNI 782 CCAGG CC^[AT]GG
BstYI 207 GGATCT [GA]^GATC[CT]
CelII 186 GCTCAGC GC^T[ACGT]AGC
DraII 784 AGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 927 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 92 CCATGG C^C[GA][CT]GG
EcoO109I 784 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRI 852 GAATTC G^AATTC
EcoRII 782 CCAGG ^CC[AT]GG
EcoT14I 92 CCATGG C^C[AT][AT]GG
EcoT22I 214 ATGCAT ATGCA^T
Fnu4HI 605 GCAGC GC^[ACGT]GC
HaeIII 509 GGCC GG^CC
HapII 873 CCGG C^CGG
HpaII 873 CCGG C^CGG
ItaI 605 GCAGC GC^[ACGT]GC
MaeI 319 CTAG C^TAG
MfeI 949 CAATTG C^AATTG
MflI 207 GGATCT [GA]^GATC[CT]
MroI 872 TCCGGA T^CCGGA
MspA1I 545 CAGCTG C[AC]G^C[GT]G
MspI 873 CCGG C^CGG
MunI 949 CAATTG C^AATTG
MvaI 782 CCAGG CC^[AT]GG
MwoI 186 GCTCAGCCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 92 CCATGG C^CATGG
NsiI 214 ATGCAT ATGCA^T
NspI 216 GCATGT [GA]CATG^[CT]
PleI 58 GACTC GAGTC(4/5)
PpuMI 784 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 779 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 545 CAGCTG CAG^CTG
RcaI 404 TCATGA T^CATGA
RsaI 597 GTAC GT^AC
SapI 48 GCTCTTC GCTCTTC(1/4)
ScrFI 782 CCAGG CC^[ACGT]GG
SfcI 828 CTACAG C^T[GA][CT]AG
SmlI 40 CTTGAG C^T[CT][GA]AG
SspBI 596 TGTACA T^GTACA
StyI 92 CCATGG C^C[AT][AT]GG
TaqI 53 TCGA T^CGA
TfiI 12 GAATC G^A[AT]TC
TseI 605 GCAGC G^C[AT]GC
TthHB8I 53 TCGA T^CGA
XhoII 207 GGATCT [GA]^GATC[CT]
XmnI 331 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AciI 363 CCGC CCGC(-3/-1)
112 GCGG
AlwI 154 GATCC GGATC(4/5)
207 GGATC
AspHI 400 GTGCTC G[AT]GC[AT]^C
792 GTGCAC
BbsI 100 GTCTTC GAAGAC(2/6)
652 GTCTTC
BpuAI 100 GTCTTC GAAGAC(2/6)
652 GTCTTC
BsaI 586 GAGACC GGTCTC(1/5)
777 GAGACC
BsiHKAI 400 GTGCTC G[AT]GC[AT]^C
792 GTGCAC
BsmFI 420 GGGAC GGGAC(10/14)
786 GTCCC
BsmI 608 GCATTC GAATGC(1/-1)
935 GCATTC
BsrI 789 CCAGT ACTGG(1/-1)
930 CCAGT
Cac8I 191 GCCAGC GC[ACGT]^[ACGT]GC
757 GCCTGC
CfoI 390 GCGC GCG^C
681 GCGC
Cfr13I 508 GGGCC G^G[ACGT]CC
785 GGTCC
EarI 49 CTCTTC CTCTTC(1/4)
902 GAAGAG
HhaI 390 GCGC GCG^C
681 GCGC
HinP1I 390 GCGC G^CGC
681 GCGC
HinfI 12 GAATC G^A[ACGT]TC
58 GACTC
Ksp632I 49 CTCTTC CTCTTC(1/4)
902 GAAGAG
MslI 573 CATCACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
672 CATTCAGATG
Sau96I 508 GGGCC G^G[ACGT]CC
785 GGTCC
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AluI BmyI BsaJI BseRI BsiYI
BslI BsmAI Bsp1286I BstF5I DdeI
DpnII FokI HphI MboI MboII
MnlI NdeII NlaIII NlaIV Sau3AI
SfaNI Tsp509I TspRI