################################## # NO CUT ENZYMES ( 192 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoT22I FauI FbaI FseI FspI HaeII HapII HhaI HinP1I HincII HindII HindIII HinfI HpaI HpaII KasI KpnI Ksp632I KspI MaeII MamI MfeI MflI MluI MluNI MroI MscI MseI MslI MspI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI TfiI Tru9I Tsp45I Tth111I TthHB8I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AcsI 122 GAATTC [GA]^AATT[CT] AcyI 268 GACGCC G[GA]^CG[CT]C AfaI 252 GTAC GT^AC AlwNI 305 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 318 GGGCCC GGGCC^C ApoI 122 GAATTC [GA]^AATT[CT] AspHI 165 GAGCAC G[AT]GC[AT]^C AvaII 243 GGTCC G^G[AT]CC AvrII 292 CCTAGG C^CTAGG BbvI 98 GCTGC GCAGC(8/12) BfaI 293 CTAG C^TAG BlnI 292 CCTAGG C^CTAGG BsaHI 268 GACGCC G[GA]^CG[CT]C BsiHKAI 165 GAGCAC G[AT]GC[AT]^C BsiYI 228 CCCCAAGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 228 CCCCAAGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BspMI 352 ACCTGC ACCTGC(4/8) BstXI 299 CCAGGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DdeI 128 CTGAG C^T[ACGT]AG DpnII 189 GATC ^GATC EcoRI 122 GAATTC G^AATTC EcoRV 332 GATATC GAT^ATC Fnu4HI 98 GCTGC GC^[ACGT]GC HgaI 268 GACGC GACGC(5/10) Hin1I 268 GACGCC G[GA]^CG[CT]C HphI 194 TCACC GGTGA(8/7) ItaI 98 GCTGC GC^[ACGT]GC MaeI 293 CTAG C^TAG MaeIII 22 GTAAC ^GT[ACGT]AC MboI 189 GATC ^GATC MspA1I 151 CAGCTG C[AC]G^C[GT]G NdeII 189 GATC ^GATC PvuII 151 CAGCTG CAG^CTG RsaI 252 GTAC GT^AC Sau3AI 189 GATC ^GATC SmlI 175 CTCAAG C^T[CT][GA]AG StuI 6 AGGCCT AGG^CCT TseI 98 GCTGC G^C[AT]GC Tsp509I 123 AATT ^AATT TspRI 305 CAGTG CA[GC]TG[ACGT][ACGT]^ ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AluI 145 AGCT AG^CT 152 AGCT BanII 72 GGGCTC G[GA]GC[CT]^C 318 GGGCCC BstF5I 63 CATCC GGATG(2/0) 84 CATCC Cac8I 4 GCAGGC GC[ACGT]^[ACGT]GC 209 GCCAGC DraII 317 AGGGCCC [GA]G^G[ACGT]CC[CT] 318 GGGCCCT EcoO109I 317 AGGGCCC [GA]G^G[ACGT]CC[CT] 318 GGGCCCT EcoT14I 39 CCTTGG C^C[AT][AT]GG 292 CCTAGG FokI 63 CATCC GGATG(9/13) 84 CATCC NlaIV 242 GGGTCC GG[ACGT]^[ACGT]CC 318 GGGCCC NspI 101 GCATGT [GA]CATG^[CT] 169 ACATGC StyI 39 CCTTGG C^C[AT][AT]GG 292 CCTAGG XcmI 210 CCAGCAGTTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 327 CCAGAGATATCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 14 enzymes) # ######################################### BmyI BsaJI Bsp1286I BssKI BstNI Cfr13I EcoRII HaeIII MboII MnlI MvaI NlaIII Sau96I ScrFI