(Circular) (Six-base) MAPSORT of: pFN21AE5063.seq Check: 2416 from: 1 to: 6256 pFN21AE5063 6256 bp With 182 enzymes: SgfI * December 8, 2014 13:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4066 278 Size: 53 83 186 936 2530 2468 Fragments arranged by size: 2530 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2010 3599 1937 Size: 73 1589 4594 Fragments arranged by size: 4594 1589 73 Acc65I G'GTAC_C Cuts at: 3577 3577 Size: 6256 AclI AA'CG_TT Cuts at: 1563 4383 4756 1563 Size: 2820 373 3063 Fragments arranged by size: 3063 2820 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3330 4319 5703 5971 1632 Size: 1698 989 1384 268 1917 Fragments arranged by size: 1917 1698 1384 989 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6059 AflIII A'CryG_T Cuts at: 1203 5155 6132 1203 Size: 3952 977 1327 Fragments arranged by size: 3952 1327 977 AgeI A'CCGG_T Cuts at: 2514 2598 5087 2514 Size: 84 2489 3683 Fragments arranged by size: 3683 2489 84 AhdI GACnn_n'nnGTC Cuts at: 4985 4985 Size: 6256 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6256 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6256 AlwNI CAG_nnn'CTG Cuts at: 3999 5571 3999 Size: 1572 4684 ApaLI G'TGCA_C Cuts at: 4313 5469 4313 Size: 1156 5100 ApoI r'AATT_y Cuts at: 1493 2125 2264 2376 2628 2712 2964 3565 3791 Size: 632 139 112 252 84 252 601 226 Cuts at: 3791 3943 6052 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 632 601 252 252 226 152 139 112 84 AseI AT'TA_AT Cuts at: 160 1032 4810 160 Size: 872 3778 1606 Fragments arranged by size: 3778 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6256 AvaI C'yCGr_G Cuts at: 1870 1943 3581 1870 Size: 73 1638 4545 Fragments arranged by size: 4545 1638 73 BamHI G'GATC_C Cuts at: 3586 3953 3586 Size: 367 5889 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3577 5032 618 Size: 325 217 627 1790 1455 1842 Fragments arranged by size: 1842 1790 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3575 1391 Size: 2184 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5285 BbvCI CC'TCA_GC Cuts at: 3109 3109 Size: 6256 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4447 4481 1903 Size: 34 2510 34 3678 Fragments arranged by size: 3678 2510 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4148 5358 4148 Size: 1210 5046 BclI T'GATC_A Cuts at: 1548 1548 Size: 6256 BfrBI ATG'CAT Cuts at: 2162 2162 Size: 6256 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4867 136 Size: 107 122 71 631 605 3195 1525 Fragments arranged by size: 3195 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6251 6251 Size: 6256 BlpI GC'TnA_GC Cuts at: 3673 3673 Size: 6256 Bme1580I G_kGCm'C Cuts at: 1790 4317 5473 1790 Size: 2527 1156 2573 Fragments arranged by size: 2573 2527 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6256 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2015 2814 3987 Size: 416 53 83 399 24 598 799 1173 Cuts at: 3987 4945 442 Size: 958 1753 Fragments arranged by size: 1753 1173 958 799 598 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6256 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2490 2574 2910 3078 3414 3498 4916 1239 Size: 1251 84 336 168 336 84 1418 2579 Fragments arranged by size: 2579 1418 1251 336 336 168 84 84 Bpu10I CC'TnA_GC Cuts at: 3109 3109 Size: 6256 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4381 5246 5544 4381 Size: 865 298 5093 Fragments arranged by size: 5093 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4919 882 Size: 617 3420 2219 Fragments arranged by size: 3420 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3377 4047 493 Size: 711 2173 670 2702 Fragments arranged by size: 2702 2173 711 670 BsaBI GATnn'nnATC Cuts at: 6250 6250 Size: 6256 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4063 4445 5080 275 Size: 53 83 186 936 2530 382 635 1451 Fragments arranged by size: 2530 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2514 2598 4689 5087 5361 5508 5919 2514 Size: 84 2091 398 274 147 411 2851 Fragments arranged by size: 2851 2091 411 398 274 147 84 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6031 Fragments arranged by size: 6031 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6256 BseYI C'CCAG_C Cuts at: 5459 5955 5459 Size: 496 5760 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3357 1163 Size: 573 1621 4062 Fragments arranged by size: 4062 1621 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3706 4467 5495 1540 Size: 275 186 1705 761 1028 2301 Fragments arranged by size: 2301 1705 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2064 4317 4402 5473 2064 Size: 2253 85 1071 2847 Fragments arranged by size: 2847 2253 1071 85 BsmI GAATG_Cn' Cuts at: 3767 3860 3767 Size: 93 6163 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2064 3575 4317 4402 5473 1391 Size: 399 274 1511 742 85 1071 2174 Fragments arranged by size: 2174 1511 1071 742 399 274 85 BspEI T'CCGG_A Cuts at: 5919 5919 Size: 6256 BspHI T'CATG_A Cuts at: 4145 5875 4145 Size: 1730 4526 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3611 844 Size: 2767 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4143 1394 Size: 429 2320 3507 Fragments arranged by size: 3507 2320 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3349 4751 4925 65 Size: 1183 2101 1402 174 1396 Fragments arranged by size: 2101 1402 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2514 2598 4900 5087 1700 Size: 110 704 84 2302 187 2869 Fragments arranged by size: 2869 2302 704 187 110 84 BsrGI T'GTAC_A Cuts at: 96 2172 96 Size: 2076 4180 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6256 BssSI C'ACGA_G Cuts at: 4316 5328 4316 Size: 1012 5244 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6256 BstBI TT'CG_AA Cuts at: 5060 5060 Size: 6256 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6256 BstYI r'GATC_y Cuts at: 1972 3586 3953 4338 4355 5807 5893 5905 6251 Size: 1614 367 385 17 1452 86 12 346 Cuts at: 6251 1972 Size: 1977 Fragments arranged by size: 1977 1614 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2329 513 Size: 552 939 325 4440 Fragments arranged by size: 4440 939 552 325 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5517 Fragments arranged by size: 5517 683 56 BtsI GCAGTG_nn' Cuts at: 3772 4566 4586 3772 Size: 794 20 5442 Fragments arranged by size: 5442 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6256 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6221 DraI TTT'AAA Cuts at: 3016 3560 3016 Size: 544 5712 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5263 809 Size: 507 3947 1802 Fragments arranged by size: 3947 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2054 3703 4592 Size: 54 1425 50 275 18 224 1649 889 Cuts at: 4592 6221 8 Size: 1629 43 Fragments arranged by size: 1649 1629 1425 889 275 224 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3703 1537 Size: 275 1891 4090 Fragments arranged by size: 4090 1891 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2634 4186 1353 Size: 1281 1552 3423 Fragments arranged by size: 3423 1552 1281 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4843 5217 5363 387 Size: 1064 138 3254 374 146 1280 Fragments arranged by size: 3254 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2490 2574 2910 3078 3330 3414 3498 Size: 393 858 84 336 168 252 84 84 Cuts at: 3498 4319 4916 5703 5971 1239 Size: 821 597 787 268 1524 Fragments arranged by size: 1524 858 821 787 597 393 336 268 252 168 84 84 84 EcoRI G'AATT_C Cuts at: 2628 2712 2964 3565 3943 2628 Size: 84 252 601 378 4941 Fragments arranged by size: 4941 601 378 252 84 FspI TGC'GCA Cuts at: 4762 4762 Size: 6256 HaeII r_GCGC'y Cuts at: 1678 5403 1678 Size: 3725 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4897 4929 4971 5003 1373 Size: 32 135 32 3325 32 42 32 2626 Fragments arranged by size: 3325 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3600 3846 669 Size: 988 281 1662 246 3079 Fragments arranged by size: 3079 1662 988 281 246 HindIII A'AGCT_T Cuts at: 748 2505 2589 3009 748 Size: 1757 84 420 3995 Fragments arranged by size: 3995 1757 420 84 HpaI GTT'AAC Cuts at: 3846 3846 Size: 6256 KpnI G_GTAC'C Cuts at: 3581 3581 Size: 6256 MfeI C'AATT_G Cuts at: 3855 3855 Size: 6256 MluI A'CGCG_T Cuts at: 6132 6132 Size: 6256 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5370 5554 1305 Size: 4065 184 2007 Fragments arranged by size: 4065 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2056 10 Size: 54 1425 343 224 4210 Fragments arranged by size: 4210 1425 343 224 54 MslI CAynn'nnrTG Cuts at: 518 1166 2151 2821 3501 4214 4573 4732 518 Size: 648 985 670 680 713 359 159 2042 Fragments arranged by size: 2042 985 713 680 670 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3672 4349 5497 5742 1606 Size: 2066 677 1148 245 2120 Fragments arranged by size: 2120 2066 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4765 Fragments arranged by size: 4765 939 552 NdeI CA'TA_TG Cuts at: 387 2104 2718 3138 387 Size: 1717 614 420 3505 Fragments arranged by size: 3505 1717 614 420 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6256 NotI GC'GGCC_GC Cuts at: 3703 3703 Size: 6256 NsiI A_TGCA'T Cuts at: 2164 2164 Size: 6256 NspI r_CATG'y Cuts at: 1132 1229 2166 2350 3614 5159 1132 Size: 97 937 184 1264 1545 2229 Fragments arranged by size: 2229 1545 1264 937 184 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6256 PciI A'CATG_T Cuts at: 5155 5155 Size: 6256 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6256 PmeI GTTT'AAAC Cuts at: 3560 3560 Size: 6256 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4325 4357 5838 5870 4325 Size: 32 1481 32 4711 Fragments arranged by size: 4711 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6256 PsiI TTA'TAA Cuts at: 2187 3826 2187 Size: 1639 4617 PstI C_TGCA'G Cuts at: 830 3608 830 Size: 2778 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6256 SalI G'TCGA_C Cuts at: 1936 3598 1936 Size: 1662 4594 SbfI CC_TGCA'GG Cuts at: 3608 3608 Size: 6256 ScaI AGT'ACT Cuts at: 4504 6216 4504 Size: 1712 4544 SfcI C'TryA_G Cuts at: 826 1045 3604 4739 5420 5611 826 Size: 219 2559 1135 681 191 1471 Fragments arranged by size: 2559 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6256 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6256 SmaI CCC'GGG Cuts at: 1872 3583 1872 Size: 1711 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4360 5261 5523 820 Size: 197 926 2417 901 262 1553 Fragments arranged by size: 2417 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4047 493 Size: 3554 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6256 SphI G_CATG'C Cuts at: 1132 2350 3614 1132 Size: 1218 1264 3774 Fragments arranged by size: 3774 1264 1218 SspI AAT'ATT Cuts at: 5 52 4180 5 Size: 47 4128 2081 Fragments arranged by size: 4128 2081 47 StuI AGG'CCT Cuts at: 1517 2675 3095 1517 Size: 1158 420 4678 Fragments arranged by size: 4678 1158 420 StyI C'CwwG_G Cuts at: 513 1065 2004 3695 513 Size: 552 939 1691 3074 Fragments arranged by size: 3074 1691 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4283 4300 4453 4638 5083 325 Size: 1255 2703 17 153 185 445 1498 Fragments arranged by size: 2703 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2172 4502 6214 Size: 274 80 33 51 1443 195 2330 1712 Cuts at: 6214 96 Size: 138 Fragments arranged by size: 2330 1712 1443 274 195 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6216 6248 6216 Size: 32 6224 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6256 XbaI T'CTAG_A Cuts at: 3592 3592 Size: 6256 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6256 XmaI C'CCGG_G Cuts at: 1870 3581 1870 Size: 1711 4545 XmnI GAAnn'nnTTC Cuts at: 2312 4385 2312 Size: 2073 4183 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4064 276 Size: 53 83 186 936 2530 2468 Fragments arranged by size: 2530 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI