################################## # NO CUT ENZYMES ( 146 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanI BbeI BbrPI BcgI BciVI BclI BfrI BglI BlnI BlpI Bpu1102I BsaHI BsaWI BseAI BsiHKAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstUI BstXI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HgaI Hin1I KasI KpnI KspI MfeI MluI MluNI MroI MscI MslI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccI 766 GTCTAC GT^[AC][GT]AC AfaI 171 GTAC GT^AC AlwI 582 GATCC GGATC(4/5) AocI 867 CCTGAGG CC^T[ACGT]AGG ApaI 252 GGGCCC GGGCC^C AvaI 54 CCCGGG C^[CT]CG[GA]G BbvCI 669 GCTGAGG CCTCAGC(-5/-2) BbvI 420 GCAGC GCAGC(8/12) BglII 293 AGATCT A^GATCT BpmI 772 CTCCAG CTGGAG(16/14) BsaAI 361 TACGTG [CT]AC^GT[GA] BsaBI 294 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 817 GGTCTC GGTCTC(1/5) BseRI 717 CTCCTC GAGGAG(10/8) BsgI 484 CTGCAC GTGCAG(16/14) BsiEI 504 CGATCG CG[GA][CT]^CG BsmI 892 GCATTC GAATGC(1/-1) BsoBI 54 CCCGGG C^[CT]CG[GA]G BsrDI 759 CATTGC GCAATG(2/0) BsrI 599 CCAGT ACTGG(1/-1) BssSI 216 CACGAG CACGAG(-5/-1) Bsu36I 867 CCTGAGG CC^T[ACGT]AGG CfoI 142 GCGC GCG^C DrdI 280 GACTGCTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 867 CCTGAGG CC^T[ACGT]AGG FauI 407 CCCGC CCCGC(4/6) Fnu4HI 420 GCAGC GC^[ACGT]GC HhaI 142 GCGC GCG^C HinP1I 142 GCGC G^CGC HindIII 701 AAGCTT A^AGCTT HpaI 4 GTTAAC GTT^AAC ItaI 420 GCAGC GC^[ACGT]GC MaeII 362 ACGT A^CGT MamI 294 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MspA1I 421 CAGCTG C[AC]G^C[GT]G PvuI 504 CGATCG CGAT^CG PvuII 421 CAGCTG CAG^CTG RsaI 171 GTAC GT^AC SfcI 747 CTACAG C^T[GA][CT]AG SmaI 54 CCCGGG CCC^GGG TaqI 549 TCGA T^CGA TseI 420 GCAGC G^C[AT]GC Tsp45I 736 GTCAC ^GT[GC]AC Tsp509I 546 AATT ^AATT TthHB8I 549 TCGA T^CGA XcmI 266 CCATCAGCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 54 CCCGGG C^CCGGG XmaI 54 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AciI 408 CCGC CCGC(-3/-1) 693 GCGG BanII 252 GGGCCC G[GA]GC[CT]^C 922 GGGCTC BfaI 109 CTAG C^TAG 451 CTAG BsmAI 640 GTCTC GTCTC(1/5) 818 GTCTC BstEII 735 GGTCACC G^GT[ACGT]ACC 882 GGTAACC BstPI 735 GGTCACC G^GT[ACGT]ACC 882 GGTAACC BstYI 293 AGATCT [GA]^GATC[CT] 581 AGATCC EcoO65I 735 GGTCACC G^GT[ACGT]ACC 882 GGTAACC HaeIII 253 GGCC GG^CC 435 GGCC HapII 22 CCGG C^CGG 55 CCGG HincII 4 GTTAAC GT[CT]^[GA]AC 241 GTCAAC HindII 4 GTTAAC GT[CT]^[GA]AC 241 GTCAAC HpaII 22 CCGG C^CGG 55 CCGG HphI 212 TCACC GGTGA(8/7) 737 TCACC MaeI 109 CTAG C^TAG 451 CTAG MaeIII 736 GTCAC ^GT[ACGT]AC 883 GTAAC MflI 293 AGATCT [GA]^GATC[CT] 581 AGATCC MspI 22 CCGG C^CGG 55 CCGG MwoI 135 GCTGTCTGCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 427 GCATCTGTGGC PflMI 231 CCAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 653 CCATTACCTGG PpuMI 533 AGGTCCC [GA]G^G[AT]CC[CT] 709 GGGACCT SfaNI 329 GCATC GCATC(5/9) 427 GCATC Van91I 231 CCAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 653 CCATTACCTGG XhoII 293 AGATCT [GA]^GATC[CT] 581 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 40 enzymes) # ######################################### AluI AvaII BbsI BcnI BmyI BpuAI BsaJI BsiYI BslI BsmFI Bsp1286I BspMI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII FokI HinfI Ksp632I MboI MboII MnlI MseI MvaI NciI NdeII NlaIV PleI Sau3AI Sau96I ScrFI Tru9I TspRI