(Circular) (Six-base) MAPSORT of: pFN21AE5975.seq Check: 7463 from: 1 to: 5641 pFN21AE5975 5641 bp With 182 enzymes: SgfI * December 8, 2014 13:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2729 2729 Size: 5641 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3451 278 Size: 53 83 186 936 1915 2468 Fragments arranged by size: 2468 1915 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2425 2984 1937 Size: 488 559 4594 Fragments arranged by size: 4594 559 488 Acc65I G'GTAC_C Cuts at: 2962 2962 Size: 5641 AclI AA'CG_TT Cuts at: 1563 3768 4141 1563 Size: 2205 373 3063 Fragments arranged by size: 3063 2205 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3704 5088 5356 1632 Size: 2072 1384 268 1917 Fragments arranged by size: 2072 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5444 AflIII A'CryG_T Cuts at: 1203 4540 5517 1203 Size: 3337 977 1327 Fragments arranged by size: 3337 1327 977 AgeI A'CCGG_T Cuts at: 4472 4472 Size: 5641 AhdI GACnn_n'nnGTC Cuts at: 4370 4370 Size: 5641 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5641 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5641 AlwNI CAG_nnn'CTG Cuts at: 3384 4956 3384 Size: 1572 4069 ApaLI G'TGCA_C Cuts at: 3698 4854 3698 Size: 1156 4485 ApoI r'AATT_y Cuts at: 1493 2950 3176 3328 5437 1493 Size: 1457 226 152 2109 1697 Fragments arranged by size: 2109 1697 1457 226 152 AseI AT'TA_AT Cuts at: 160 1032 4195 160 Size: 872 3163 1606 Fragments arranged by size: 3163 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5641 AvaI C'yCGr_G Cuts at: 1870 1943 2966 1870 Size: 73 1023 4545 Fragments arranged by size: 4545 1023 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2644 2677 2644 Size: 33 5608 BamHI G'GATC_C Cuts at: 2682 2971 3338 2682 Size: 289 367 4985 Fragments arranged by size: 4985 367 289 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2268 2324 2759 2926 2962 Size: 325 217 627 481 56 435 167 36 Cuts at: 2962 4417 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 481 435 325 217 167 56 36 BanII G_rGCy'C Cuts at: 1391 2651 2960 1391 Size: 1260 309 4072 Fragments arranged by size: 4072 1260 309 BbeI G_GCGC'C Cuts at: 2272 2328 2272 Size: 56 5585 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4670 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3832 3866 1903 Size: 34 1895 34 3678 Fragments arranged by size: 3678 1895 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2672 3533 4743 2672 Size: 861 1210 3570 Fragments arranged by size: 3570 1210 861 BclI T'GATC_A Cuts at: 1548 2736 1548 Size: 1188 4453 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2634 4252 136 Size: 107 122 71 631 605 962 1618 1525 Fragments arranged by size: 1618 1525 962 631 605 122 107 71 BglII A'GATC_T Cuts at: 5636 5636 Size: 5641 BlpI GC'TnA_GC Cuts at: 2408 3058 2408 Size: 650 4991 Bme1580I G_kGCm'C Cuts at: 1790 3702 4858 1790 Size: 1912 1156 2573 Fragments arranged by size: 2573 1912 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5641 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3372 4330 442 Size: 416 53 83 399 24 1955 958 1753 Fragments arranged by size: 1955 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2435 1056 Size: 1379 4262 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2054 2150 2282 4301 1239 Size: 815 96 132 2019 2579 Fragments arranged by size: 2579 2019 815 132 96 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3766 4631 4929 3766 Size: 865 298 4478 Fragments arranged by size: 4478 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2152 2749 4304 882 Size: 617 653 597 1555 2219 Fragments arranged by size: 2219 1555 653 617 597 BsaAI yAC'GTr Cuts at: 493 1204 3432 493 Size: 711 2228 2702 Fragments arranged by size: 2702 2228 711 BsaBI GATnn'nnATC Cuts at: 5635 5635 Size: 5641 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2269 2325 3448 3830 Size: 53 83 186 936 736 56 1123 382 Cuts at: 3830 4465 275 Size: 635 1451 Fragments arranged by size: 1451 1123 936 736 635 382 186 83 56 53 BsaWI w'CCGG_w Cuts at: 2679 4074 4472 4746 4893 5304 2679 Size: 1395 398 274 147 411 3016 Fragments arranged by size: 3016 1395 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5416 Fragments arranged by size: 5416 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2606 2716 1186 Size: 1420 110 4111 Fragments arranged by size: 4111 1420 110 BseYI C'CCAG_C Cuts at: 2435 2470 4844 5340 2435 Size: 35 2374 496 2736 Fragments arranged by size: 2736 2374 496 35 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5068 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3091 3852 4880 1540 Size: 275 186 1090 761 1028 2301 Fragments arranged by size: 2301 1090 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2296 3702 3787 4858 2296 Size: 1406 85 1071 3079 Fragments arranged by size: 3079 1406 1071 85 BsmI GAATG_Cn' Cuts at: 3152 3245 3152 Size: 93 5548 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2296 2651 2960 3702 3787 4858 1391 Size: 399 506 355 309 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 506 399 355 309 85 BspEI T'CCGG_A Cuts at: 2679 5304 2679 Size: 2625 3016 BspHI T'CATG_A Cuts at: 3530 5260 3530 Size: 1730 3911 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2115 2729 2996 844 Size: 1271 614 267 3489 Fragments arranged by size: 3489 1271 614 267 BsrBI CCG'CTC Cuts at: 1394 1823 3528 1394 Size: 429 1705 3507 Fragments arranged by size: 3507 1705 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4136 4310 65 Size: 1183 2888 174 1396 Fragments arranged by size: 2888 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2265 4285 4472 1700 Size: 110 455 2020 187 2869 Fragments arranged by size: 2869 2020 455 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5641 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5641 BssSI C'ACGA_G Cuts at: 3701 4713 3701 Size: 1012 4629 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5641 BstBI TT'CG_AA Cuts at: 4445 4445 Size: 5641 BstEII G'GTnAC_C Cuts at: 1184 2842 1184 Size: 1658 3983 BstYI r'GATC_y Cuts at: 1972 2541 2682 2971 3338 3723 3740 5192 5278 Size: 569 141 289 367 385 17 1452 86 Cuts at: 5278 5290 5636 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 569 385 367 346 289 141 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2787 2787 Size: 5641 BtgI C'CryG_G Cuts at: 513 1065 2022 2115 2328 2352 513 Size: 552 957 93 213 24 3802 Fragments arranged by size: 3802 957 552 213 93 24 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4902 Fragments arranged by size: 4902 683 56 BtsI GCAGTG_nn' Cuts at: 2399 3157 3951 3971 2399 Size: 758 794 20 4069 Fragments arranged by size: 4069 794 758 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5641 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5606 DraI TTT'AAA Cuts at: 2945 2945 Size: 5641 DraIII CAC_nnn'GTG Cuts at: 2334 2419 2334 Size: 85 5556 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4648 809 Size: 507 3332 1802 Fragments arranged by size: 3332 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2061 2263 2805 Size: 54 1425 50 275 18 231 202 542 Cuts at: 2805 3088 3977 5606 8 Size: 283 889 1629 43 Fragments arranged by size: 1629 1425 889 542 283 275 231 202 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3088 1537 Size: 275 1276 4090 Fragments arranged by size: 4090 1276 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2087 2321 2756 2834 3571 1353 Size: 734 234 435 78 737 3423 Fragments arranged by size: 3423 737 734 435 234 78 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4228 4602 4748 387 Size: 1064 138 2639 374 146 1280 Fragments arranged by size: 2639 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2054 2150 2282 3704 4301 5088 5356 Size: 393 422 96 132 1422 597 787 268 Cuts at: 5356 1239 Size: 1524 Fragments arranged by size: 1524 1422 787 597 422 393 268 132 96 EcoRI G'AATT_C Cuts at: 2950 3328 2950 Size: 378 5263 FspI TGC'GCA Cuts at: 2192 4147 2192 Size: 1955 3686 HaeII r_GCGC'y Cuts at: 1678 2272 2328 4788 1678 Size: 594 56 2460 2531 Fragments arranged by size: 2531 2460 594 56 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2280 2312 4282 4314 4356 Size: 32 135 32 708 32 1970 32 42 Cuts at: 4356 4388 1373 Size: 32 2626 Fragments arranged by size: 2626 1970 708 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2985 3231 669 Size: 988 281 1047 246 3079 Fragments arranged by size: 3079 1047 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5641 HpaI GTT'AAC Cuts at: 3231 3231 Size: 5641 KasI G'GCGC_C Cuts at: 2268 2324 2268 Size: 56 5585 KpnI G_GTAC'C Cuts at: 2966 2966 Size: 5641 MfeI C'AATT_G Cuts at: 3240 3240 Size: 5641 MluI A'CGCG_T Cuts at: 5517 5517 Size: 5641 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4755 4939 1305 Size: 3450 184 2007 Fragments arranged by size: 3450 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3819 Fragments arranged by size: 3819 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2021 2138 3599 3958 4117 518 Size: 648 855 117 1461 359 159 2042 Fragments arranged by size: 2042 1461 855 648 359 159 117 MspA1I CmG'CkG Cuts at: 1606 2560 3057 3734 4882 5127 1606 Size: 954 497 677 1148 245 2120 Fragments arranged by size: 2120 1148 954 677 497 245 NaeI GCC'GGC Cuts at: 2267 2267 Size: 5641 NarI GG'CG_CC Cuts at: 2269 2325 2269 Size: 56 5585 NcoI C'CATG_G Cuts at: 513 1065 2022 2352 513 Size: 552 957 330 3802 Fragments arranged by size: 3802 957 552 330 NdeI CA'TA_TG Cuts at: 387 387 Size: 5641 NgoMIV G'CCGG_C Cuts at: 2265 2265 Size: 5641 NheI G'CTAG_C Cuts at: 1052 2431 1052 Size: 1379 4262 NotI GC'GGCC_GC Cuts at: 3088 3088 Size: 5641 NspI r_CATG'y Cuts at: 1132 1229 2999 4544 1132 Size: 97 1770 1545 2229 Fragments arranged by size: 2229 1770 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5641 PciI A'CATG_T Cuts at: 4540 4540 Size: 5641 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5641 PmeI GTTT'AAAC Cuts at: 2945 2945 Size: 5641 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3710 3742 5223 5255 3710 Size: 32 1481 32 4096 Fragments arranged by size: 4096 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5641 PsiI TTA'TAA Cuts at: 3211 3211 Size: 5641 PstI C_TGCA'G Cuts at: 830 2559 2595 2993 830 Size: 1729 36 398 3478 Fragments arranged by size: 3478 1729 398 36 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5641 PvuII CAG'CTG Cuts at: 2560 2560 Size: 5641 SalI G'TCGA_C Cuts at: 1936 2983 1936 Size: 1047 4594 SapI GCTCTTCn'nnn_ Cuts at: 2087 2087 Size: 5641 SbfI CC_TGCA'GG Cuts at: 2993 2993 Size: 5641 ScaI AGT'ACT Cuts at: 3889 5601 3889 Size: 1712 3929 SfcI C'TryA_G Cuts at: 826 1045 2426 2555 2591 2869 2989 4124 4805 Size: 219 1381 129 36 278 120 1135 681 Cuts at: 4805 4996 826 Size: 191 1471 Fragments arranged by size: 1471 1381 1135 681 278 219 191 129 120 36 SfoI GGC'GCC Cuts at: 2270 2326 2270 Size: 56 5585 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5641 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5641 SmaI CCC'GGG Cuts at: 1872 2968 1872 Size: 1096 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3745 4646 4908 820 Size: 197 926 1802 901 262 1553 Fragments arranged by size: 1802 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3432 493 Size: 2939 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5641 SphI G_CATG'C Cuts at: 1132 2999 1132 Size: 1867 3774 SspI AAT'ATT Cuts at: 5 52 3565 5 Size: 47 3513 2081 Fragments arranged by size: 3513 2081 47 StuI AGG'CCT Cuts at: 1517 2711 1517 Size: 1194 4447 StyI C'CwwG_G Cuts at: 513 1065 2022 2205 2352 3080 513 Size: 552 957 183 147 728 3074 Fragments arranged by size: 3074 957 728 552 183 147 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3668 3685 3838 4023 4468 325 Size: 1255 2088 17 153 185 445 1498 Fragments arranged by size: 2088 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2178 3887 5599 Size: 274 80 33 51 1443 201 1709 1712 Cuts at: 5599 96 Size: 138 Fragments arranged by size: 1712 1709 1443 274 201 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5601 5633 5601 Size: 32 5609 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5641 XbaI T'CTAG_A Cuts at: 2977 2977 Size: 5641 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2258 2815 2258 Size: 557 5084 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5641 XmaI C'CCGG_G Cuts at: 1870 2966 1870 Size: 1096 4545 XmnI GAAnn'nnTTC Cuts at: 3770 3770 Size: 5641 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3449 276 Size: 53 83 186 936 1915 2468 Fragments arranged by size: 2468 1915 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI