################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflIII AgeI AhdI AlwI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BseAI BsiEI BsiWI BsmBI BspDI BspEI BspLU11I BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DpnII DraIII DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoT22I FbaI FseI FspI HaeII HgaI Hin1I HindIII HpaI KasI KpnI KspI MamI MboI MflI MluI MluNI MroI MscI MvnI NaeI NarI NcoI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI Sau3AI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI StuI SwaI Tth111I XbaI XhoI XhoII ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccI 1828 GTCTAC GT^[AC][GT]AC AflII 1044 CTTAAG C^TTAAG AlwNI 284 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 609 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 891 CCCGGG C^[CT]CG[GA]G BanI 1247 GGCACC G^G[CT][GA]CC BanII 1098 GAGCTC G[GA]GC[CT]^C BfaI 1073 CTAG C^TAG BfrI 1044 CTTAAG C^TTAAG BsoBI 891 CCCGGG C^[CT]CG[GA]G Bsp1407I 116 TGTACA T^GTACA BspHI 1443 TCATGA T^CATGA BsrDI 306 GCAATG GCAATG(2/0) BsrGI 116 TGTACA T^GTACA BstEII 1554 GGTAACC G^GT[ACGT]ACC BstPI 1554 GGTAACC G^GT[ACGT]ACC CfoI 1335 GCGC GCG^C DraI 1353 TTTAAA TTT^AAA DraII 1283 GGGTCCT [GA]G^G[ACGT]CC[CT] DrdI 1135 GACAACTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 1513 CCTTCCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 1283 GGGTCCT [GA]G^G[ACGT]CC[CT] EcoO65I 1554 GGTAACC G^GT[ACGT]ACC EcoRI 1489 GAATTC G^AATTC EcoT14I 163 CCAAGG C^C[AT][AT]GG FauI 1808 CCCGC CCCGC(4/6) HaeIII 1205 GGCC GG^CC HhaI 1335 GCGC GCG^C HinP1I 1335 GCGC G^CGC HincII 898 GTTGAC GT[CT]^[GA]AC HindII 898 GTTGAC GT[CT]^[GA]AC MaeI 1073 CTAG C^TAG MaeII 551 ACGT A^CGT MfeI 492 CAATTG C^AATTG MspA1I 1788 CAGCTG C[AC]G^C[GT]G MunI 492 CAATTG C^AATTG NdeI 452 CATATG CA^TATG PpuMI 1283 GGGTCCT [GA]G^G[AT]CC[CT] PvuII 1788 CAGCTG CAG^CTG RcaI 1443 TCATGA T^CATGA SacI 1098 GAGCTC GAGCT^C SexAI 1821 ACCTGGT A^CC[AT]GGT SmaI 891 CCCGGG CCC^GGG SspBI 116 TGTACA T^GTACA StyI 163 CCAAGG C^C[AT][AT]GG XcmI 1299 CCACTGCTTTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 891 CCCGGG C^CCGGG XmaI 891 CCCGGG C^CCGGG XmnI 609 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AciI 530 CCGC CCGC(-3/-1) 1809 CCGC AcsI 884 AAATTT [GA]^AATT[CT] 1489 GAATTC Alw44I 150 GTGCAC G^TGCAC 420 GTGCAC ApaLI 150 GTGCAC G^TGCAC 420 GTGCAC ApoI 884 AAATTT [GA]^AATT[CT] 1489 GAATTC BbsI 1012 GAAGAC GAAGAC(2/6) 767 GTCTTC BbvI 437 GCTGC GCAGC(8/12) 1294 GCTGC BpmI 662 CTCCAG CTGGAG(16/14) 1465 CTCCAG BpuAI 1012 GAAGAC GAAGAC(2/6) 767 GTCTTC BsaWI 485 ACCGGA [AT]^CCGG[AT] 1558 ACCGGA BsmI 406 GAATGC GAATGC(1/-1) 1639 GAATGC EcoRV 1447 GATATC GAT^ATC 1652 GATATC Fnu4HI 437 GCTGC GC^[ACGT]GC 1294 GCTGC ItaI 437 GCTGC GC^[ACGT]GC 1294 GCTGC MslI 966 CACAAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1249 CACCTGTGTG PflMI 1544 CCAACTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1674 CCAACGGATGG PstI 1222 CTGCAG CTGCA^G 1321 CTGCAG SbfI 1221 CCTGCAGG CCTGCA^GG 1320 CCTGCAGG SmlI 812 CTCAAG C^T[CT][GA]AG 1044 CTTAAG Sse8387I 1221 CCTGCAGG CCTGCA^GG 1320 CCTGCAGG SspI 1597 AATATT AAT^ATT 1615 AATATT TseI 437 GCTGC G^C[AT]GC 1294 GCTGC Van91I 1544 CCAACTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1674 CCAACGGATGG ######################################### # 3 OR MORE CUT ENZYMES ( 54 enzymes) # ######################################### AfaI AluI AspHI AvaII BcnI BmyI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI EarI EcoRII FokI HapII HinfI HpaII HphI Ksp632I MaeIII MboII MnlI MseI MspI MvaI MwoI NciI NlaIII NlaIV PleI RsaI Sau96I ScrFI SfaNI SfcI TaqI TfiI Tru9I Tsp45I Tsp509I TspRI TthHB8I