(Linear) (Six-base) MAPSORT of: KIEE2460.seq Check: 2317 from: 1 to: 1695 KIEE2460.orf With 182 enzymes: SgfI * March 29, 2013 15:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1333 1695 Size: 1333 362 Acc65I G'GTAC_C Cuts at: 0 820 1695 Size: 820 875 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 304 773 1695 Size: 304 469 922 Fragments arranged by size: 922 469 304 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 726 759 1695 Size: 726 33 936 Fragments arranged by size: 936 726 33 BanI G'GyrC_C Cuts at: 0 449 820 1513 1695 Size: 449 371 693 182 Fragments arranged by size: 693 449 371 182 BanII G_rGCy'C Cuts at: 0 147 1013 1695 Size: 147 866 682 Fragments arranged by size: 866 682 147 BbsI GAAGACnn'nnnn_ Cuts at: 0 898 1599 1695 Size: 898 701 96 Fragments arranged by size: 898 701 96 BciVI GTATCCnnnnn_n' Cuts at: 0 39 727 1695 Size: 39 688 968 Fragments arranged by size: 968 688 39 BfrBI ATG'CAT Cuts at: 0 1428 1695 Size: 1428 267 BglII A'GATC_T Cuts at: 0 264 1695 Size: 264 1431 Bme1580I G_kGCm'C Cuts at: 0 390 460 1518 1695 Size: 390 70 1058 177 Fragments arranged by size: 1058 390 177 70 BmrI ACTGGGnnnn_n' Cuts at: 0 1329 1646 1695 Size: 1329 317 49 Fragments arranged by size: 1329 317 49 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1076 1474 1578 1695 Size: 1076 398 104 117 Fragments arranged by size: 1076 398 117 104 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 366 1695 Size: 366 1329 BsaAI yAC'GTr Cuts at: 0 703 1695 Size: 703 992 BsaWI w'CCGG_w Cuts at: 0 1054 1695 Size: 1054 641 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 483 513 1695 Size: 483 30 1182 Fragments arranged by size: 1182 483 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1160 1223 1695 Size: 1160 63 472 Fragments arranged by size: 1160 472 63 BseYI C'CCAG_C Cuts at: 0 134 140 1338 1695 Size: 134 6 1198 357 Fragments arranged by size: 1198 357 134 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 457 693 1238 1379 1459 1695 Size: 457 236 545 141 80 236 Fragments arranged by size: 545 457 236 236 141 80 BsiEI CG_ry'CG Cuts at: 0 53 1419 1695 Size: 53 1366 276 Fragments arranged by size: 1366 276 53 BsiHKAI G_wGCw'C Cuts at: 0 1013 1695 Size: 1013 682 BsiWI C'GTAC_G Cuts at: 0 699 1695 Size: 699 996 BsmI GAATG_Cn' Cuts at: 0 655 1428 1695 Size: 655 773 267 Fragments arranged by size: 773 655 267 Bsp1286I G_dGCh'C Cuts at: 0 147 390 460 1013 1518 1695 Size: 147 243 70 553 505 177 Fragments arranged by size: 553 505 243 177 147 70 BspHI T'CATG_A Cuts at: 0 636 1695 Size: 636 1059 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 50 403 1333 1695 Size: 50 353 930 362 Fragments arranged by size: 930 362 353 50 BsrDI GCAATG_nn' Cuts at: 0 559 1695 Size: 559 1136 BstAPI GCAn_nnn'nTGC Cuts at: 0 1078 1695 Size: 1078 617 BstXI CCAn_nnnn'nTGG Cuts at: 0 745 1695 Size: 745 950 BstYI r'GATC_y Cuts at: 0 264 1695 Size: 264 1431 BtgI C'CryG_G Cuts at: 0 401 1162 1275 1518 1551 1695 Size: 401 761 113 243 33 144 Fragments arranged by size: 761 401 243 144 113 33 BtsI GCAGTG_nn' Cuts at: 0 1677 1695 Size: 1677 18 ClaI AT'CG_AT Cuts at: 0 94 1695 Size: 94 1601 DraI TTT'AAA Cuts at: 0 830 1695 Size: 830 865 DraIII CAC_nnn'GTG Cuts at: 0 1464 1695 Size: 1464 231 EaeI y'GGCC_r Cuts at: 0 50 1416 1548 1695 Size: 50 1366 132 147 Fragments arranged by size: 1366 147 132 50 EagI C'GGCC_G Cuts at: 0 50 1416 1695 Size: 50 1366 279 Fragments arranged by size: 1366 279 50 EarI CTCTTCn'nnn_ Cuts at: 0 522 1695 Size: 522 1173 EcoICRI GAG'CTC Cuts at: 0 1011 1695 Size: 1011 684 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 304 773 1076 1474 1578 1695 Size: 304 469 303 398 104 117 Fragments arranged by size: 469 398 304 303 117 104 EcoNI CCTnn'n_nnAGG Cuts at: 0 1094 1695 Size: 1094 601 EcoO109I rG'GnC_Cy Cuts at: 0 494 707 1335 1437 1695 Size: 494 213 628 102 258 Fragments arranged by size: 628 494 258 213 102 HaeII r_GCGC'y Cuts at: 0 19 1695 Size: 19 1676 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 344 376 637 669 1116 1148 1695 Size: 344 32 261 32 447 32 547 Fragments arranged by size: 547 447 344 261 32 32 32 HincII GTy'rAC Cuts at: 0 561 1110 1695 Size: 561 549 585 Fragments arranged by size: 585 561 549 KpnI G_GTAC'C Cuts at: 0 824 1695 Size: 824 871 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 849 1695 Size: 849 846 MscI TGG'CCA Cuts at: 0 1550 1695 Size: 1550 145 MspA1I CmG'CkG Cuts at: 0 1078 1695 Size: 1078 617 NcoI C'CATG_G Cuts at: 0 401 1275 1551 1695 Size: 401 874 276 144 Fragments arranged by size: 874 401 276 144 NotI GC'GGCC_GC Cuts at: 0 50 1695 Size: 50 1645 NsiI A_TGCA'T Cuts at: 0 1430 1695 Size: 1430 265 PasI CC'CwG_GG Cuts at: 0 1381 1695 Size: 1381 314 PflMI CCAn_nnn'nTGG Cuts at: 0 466 1345 1695 Size: 466 879 350 Fragments arranged by size: 879 466 350 PfoI T'CCnGG_A Cuts at: 0 1092 1695 Size: 1092 603 PpuMI rG'GwC_Cy Cuts at: 0 494 707 1335 1437 1695 Size: 494 213 628 102 258 Fragments arranged by size: 628 494 258 213 102 PsiI TTA'TAA Cuts at: 0 695 1695 Size: 695 1000 PstI C_TGCA'G Cuts at: 0 47 1695 Size: 47 1648 PvuII CAG'CTG Cuts at: 0 1078 1695 Size: 1078 617 SacI G_AGCT'C Cuts at: 0 1013 1695 Size: 1013 682 SanDI GG'GwC_CC Cuts at: 0 1335 1695 Size: 1335 360 SapI GCTCTTCn'nnn_ Cuts at: 0 522 1695 Size: 522 1173 SbfI CC_TGCA'GG Cuts at: 0 47 1695 Size: 47 1648 ScaI AGT'ACT Cuts at: 0 876 1695 Size: 876 819 SfcI C'TryA_G Cuts at: 0 43 521 1067 1695 Size: 43 478 546 628 Fragments arranged by size: 628 546 478 43 SmlI C'TyrA_G Cuts at: 0 381 1695 Size: 381 1314 SspI AAT'ATT Cuts at: 0 1193 1695 Size: 1193 502 StuI AGG'CCT Cuts at: 0 927 1695 Size: 927 768 StyI C'CwwG_G Cuts at: 0 401 1275 1551 1695 Size: 401 874 276 144 Fragments arranged by size: 874 401 276 144 TatI w'GTAC_w Cuts at: 0 874 1084 1695 Size: 874 210 611 Fragments arranged by size: 874 611 210 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 483 515 1695 Size: 483 32 1180 Fragments arranged by size: 1180 483 32 Tth111I GACn'n_nGTC Cuts at: 0 164 1362 1695 Size: 164 1198 333 Fragments arranged by size: 1198 333 164 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1282 1695 Size: 1282 413 Enzymes that do cut: AarI Acc65I AcuI BaeI BanI BanII BbsI BciVI BfrBI BglII Bme1580I BmrI BpmI BpuEI BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspHI BspMI BsrDI BstAPI BstXI BstYI BtgI BtsI ClaI DraI DraIII EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KpnI MmeI MscI MspA1I NcoI NotI NsiI PasI PflMI PfoI PpuMI PsiI PstI PvuII SacI SanDI SapI SbfI ScaI SfcI SmlI SspI StuI StyI TatI TstI Tth111I XcmI Enzymes that do not cut: AatII AccI AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BamHI BbeI BbvCI BcgI BclI BglI BlpI BmgBI BmtI BplI Bpu10I BsaI BsaBI BsaHI BsmBI BspEI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI CspCI DrdI EciI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KasI MfeI MluI MslI NaeI NarI NdeI NgoMIV NheI NruI NspI PacI PciI PmeI PmlI PpiI PshAI PspOMI PspXI PsrI PvuI RsrII SacII SalI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI TaqII XbaI XhoI XmaI XmnI ZraI