################################## # NO CUT ENZYMES ( 185 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DdeI DpnII DraI DraII DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FbaI FseI FspI HgaI Hin1I HincII HindII HinfI HpaI KasI KpnI Ksp632I KspI MaeI MaeII MaeIII MamI MboI MboII MfeI MflI MluI MroI MslI MspA1I MunI NarI NdeI NdeII NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI Sau3AI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI SspBI SspI StuI SwaI TfiI Tsp45I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AcsI 152 GAATTC [GA]^AATT[CT] AluI 249 AGCT AG^CT AlwNI 139 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 152 GAATTC [GA]^AATT[CT] AvaII 196 GGACC G^G[AT]CC BalI 110 TGGCCA TGG^CCA BsmAI 254 GTCTC GTCTC(1/5) BsmFI 189 GTCCC GGGAC(10/14) BsrFI 86 GCCGGC [GA]^CCGG[CT] BsrI 129 CCAGT ACTGG(1/-1) BssHII 90 GCGCGC G^CGCGC BstAPI 138 GCAGACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 168 CATCC GGATG(2/0) Cfr10I 86 GCCGGC [GA]^CCGG[CT] DsaI 272 CCATGG C^C[GA][CT]GG EcoRI 152 GAATTC G^AATTC EcoT14I 272 CCATGG C^C[AT][AT]GG FokI 168 CATCC GGATG(9/13) HindIII 248 AAGCTT A^AGCTT MluNI 110 TGGCCA TGG^CCA MnlI 159 CCTC CCTC(7/6) MscI 110 TGGCCA TGG^CCA MseI 278 TTAA T^TAA NaeI 86 GCCGGC GCC^GGC NcoI 272 CCATGG C^CATGG NgoMIV 86 GCCGGC G^CCGGC NlaIII 273 CATG CATG^ PstI 145 CTGCAG CTGCA^G SbfI 144 CCTGCAGG CCTGCA^GG SfcI 145 CTGCAG C^T[GA][CT]AG Sse8387I 144 CCTGCAGG CCTGCA^GG StyI 272 CCATGG C^C[AT][AT]GG TaqI 290 TCGA T^CGA Tru9I 278 TTAA T^TAA Tsp509I 153 AATT ^AATT TthHB8I 290 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AccII 7 CGCG CG^CG 91 CGCG BcnI 38 CCCGG CC^[GC]GG 193 CCCGG BstNI 72 CCTGG CC^[AT]GG 108 CCTGG BstUI 7 CGCG CG^CG 91 CGCG Cfr13I 3 GGCCC G^G[ACGT]CC 196 GGACC EaeI 77 TGGCCG [CT]^GGCC[GA] 110 TGGCCA EcoRII 72 CCTGG ^CC[AT]GG 108 CCTGG HaeII 54 GGCGCT [GA]GCGC^[CT] 102 AGCGCC HphI 183 GGTGA GGTGA(8/7) 156 TCACC MvaI 72 CCTGG CC^[AT]GG 108 CCTGG MvnI 7 CGCG CG^CG 91 CGCG NciI 38 CCCGG CC^[GC]GG 193 CCCGG NlaIV 41 GGCTCC GG[ACGT]^[ACGT]CC 196 GGACCC Sau96I 3 GGCCC G^G[ACGT]CC 196 GGACC SfaNI 297 GATGC GCATC(5/9) 265 GCATC SmlI 160 CTCAAG C^T[CT][GA]AG 232 CTCAAG ######################################### # 3 OR MORE CUT ENZYMES ( 21 enzymes) # ######################################### AciI BbvI BsaJI BsiYI BslI BssKI Cac8I CfoI FauI Fnu4HI HaeIII HapII HhaI HinP1I HpaII ItaI MspI MwoI ScrFI TseI TspRI