(Circular) (Six-base) MAPSORT of: pFN21AE3405.seq Check: 8536 from: 1 to: 5341 pFN21AE3405 5341 bp With 182 enzymes: SgfI * March 28, 2012 12:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2489 3151 278 Size: 53 83 186 936 953 662 2468 Fragments arranged by size: 2468 953 936 662 186 83 53 AccI GT'mk_AC Cuts at: 1937 2163 2684 1937 Size: 226 521 4594 Fragments arranged by size: 4594 521 226 Acc65I G'GTAC_C Cuts at: 2662 2662 Size: 5341 AclI AA'CG_TT Cuts at: 1563 3468 3841 1563 Size: 1905 373 3063 Fragments arranged by size: 3063 1905 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3404 4788 5056 1632 Size: 1772 1384 268 1917 Fragments arranged by size: 1917 1772 1384 268 AfeI AGC'GCT Cuts at: 2114 2114 Size: 5341 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5144 AflIII A'CryG_T Cuts at: 1203 4240 5217 1203 Size: 3037 977 1327 Fragments arranged by size: 3037 1327 977 AgeI A'CCGG_T Cuts at: 4172 4172 Size: 5341 AhdI GACnn_n'nnGTC Cuts at: 4070 4070 Size: 5341 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5341 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5341 AlwNI CAG_nnn'CTG Cuts at: 2386 2449 3084 4656 2386 Size: 63 635 1572 3071 Fragments arranged by size: 3071 1572 635 63 ApaI G_GGCC'C Cuts at: 2021 2564 2021 Size: 543 4798 ApaLI G'TGCA_C Cuts at: 3398 4554 3398 Size: 1156 4185 ApoI r'AATT_y Cuts at: 1493 2650 2876 3028 5137 1493 Size: 1157 226 152 2109 1697 Fragments arranged by size: 2109 1697 1157 226 152 AseI AT'TA_AT Cuts at: 160 1032 3895 160 Size: 872 2863 1606 Fragments arranged by size: 2863 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5341 AvaI C'yCGr_G Cuts at: 1870 1943 2026 2500 2666 1870 Size: 73 83 474 166 4545 Fragments arranged by size: 4545 474 166 83 73 BamHI G'GATC_C Cuts at: 2671 3038 2671 Size: 367 4974 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2126 2329 2662 4117 618 Size: 325 217 627 339 203 333 1455 1842 Fragments arranged by size: 1842 1455 627 339 333 325 217 203 BanII G_rGCy'C Cuts at: 1391 2021 2318 2369 2544 2564 2585 2660 1391 Size: 630 297 51 175 20 21 75 4072 Fragments arranged by size: 4072 630 297 175 75 51 21 20 BbeI G_GCGC'C Cuts at: 2130 2130 Size: 5341 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2183 928 Size: 971 284 4086 Fragments arranged by size: 4086 971 284 BbvCI CC'TCA_GC Cuts at: 2408 2436 2408 Size: 28 5313 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2596 2630 3532 3566 1903 Size: 34 659 34 902 34 3678 Fragments arranged by size: 3678 902 659 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3233 4443 3233 Size: 1210 4131 BclI T'GATC_A Cuts at: 1548 1548 Size: 5341 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2282 3952 136 Size: 107 122 71 631 605 610 1670 1525 Fragments arranged by size: 1670 1525 631 610 605 122 107 71 BglII A'GATC_T Cuts at: 2214 5336 2214 Size: 3122 2219 BlpI GC'TnA_GC Cuts at: 2454 2758 2454 Size: 304 5037 Bme1580I G_kGCm'C Cuts at: 1790 2021 2564 3402 4558 1790 Size: 231 543 838 1156 2573 Fragments arranged by size: 2573 1156 838 543 231 BmgBI CAC'GTC Cuts at: 1318 2100 1318 Size: 782 4559 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3072 4030 442 Size: 416 53 83 399 24 1655 958 1753 Fragments arranged by size: 1753 1655 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5341 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2204 4001 1239 Size: 965 1797 2579 Fragments arranged by size: 2579 1797 965 Bpu10I CC'TnA_GC Cuts at: 2408 2436 2408 Size: 28 5313 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3466 4331 4629 3466 Size: 865 298 4178 Fragments arranged by size: 4178 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2431 4004 882 Size: 617 932 1573 2219 Fragments arranged by size: 2219 1573 932 617 BsaAI yAC'GTr Cuts at: 493 1204 3132 493 Size: 711 1928 2702 Fragments arranged by size: 2702 1928 711 BsaBI GATnn'nnATC Cuts at: 5335 5335 Size: 5341 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2127 2160 2486 3148 Size: 53 83 186 936 594 33 326 662 Cuts at: 3148 3530 4165 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 662 635 594 382 326 186 83 53 33 BsaWI w'CCGG_w Cuts at: 3774 4172 4446 4593 5004 3774 Size: 398 274 147 411 4111 Fragments arranged by size: 4111 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5116 Fragments arranged by size: 5116 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2326 2528 1186 Size: 1140 202 3999 Fragments arranged by size: 3999 1140 202 BseYI C'CCAG_C Cuts at: 2625 4544 5040 2625 Size: 1919 496 2926 Fragments arranged by size: 2926 1919 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2129 2399 1163 Size: 573 393 270 4105 Fragments arranged by size: 4105 573 393 270 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2229 2791 3552 4580 1540 Size: 275 186 228 562 761 1028 2301 Fragments arranged by size: 2301 1028 761 562 275 228 186 BsiHKAI G_wGCw'C Cuts at: 2318 2460 2544 3402 3487 4558 2318 Size: 142 84 858 85 1071 3101 Fragments arranged by size: 3101 1071 858 142 85 84 BsmI GAATG_Cn' Cuts at: 2852 2945 2852 Size: 93 5248 BsmBI CGTCTCn'nnnn_ Cuts at: 2477 2477 Size: 5341 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2021 2318 2369 2460 2544 2564 2585 Size: 399 231 297 51 91 84 20 21 Cuts at: 2585 2660 3402 3487 4558 1391 Size: 75 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 297 231 91 85 84 75 51 21 20 BspEI T'CCGG_A Cuts at: 5004 5004 Size: 5341 BspHI T'CATG_A Cuts at: 3230 4960 3230 Size: 1730 3611 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2696 844 Size: 1852 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2239 3228 1394 Size: 429 416 989 3507 Fragments arranged by size: 3507 989 429 416 BsrDI GCAATG_nn' Cuts at: 65 1248 3836 4010 65 Size: 1183 2588 174 1396 Fragments arranged by size: 2588 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2123 2228 3985 4172 1700 Size: 110 313 105 1757 187 2869 Fragments arranged by size: 2869 1757 313 187 110 105 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5341 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5341 BssSI C'ACGA_G Cuts at: 3401 4413 3401 Size: 1012 4329 BstAPI GCAn_nnn'nTGC Cuts at: 794 2100 794 Size: 1306 4035 BstBI TT'CG_AA Cuts at: 4145 4145 Size: 5341 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5341 BstYI r'GATC_y Cuts at: 1972 2187 2214 2671 3038 3423 3440 4892 4978 Size: 215 27 457 367 385 17 1452 86 Cuts at: 4978 4990 5336 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 457 385 367 346 215 86 27 17 12 Bsu36I CC'TnA_GG Cuts at: 2525 2525 Size: 5341 BtgI C'CryG_G Cuts at: 513 1065 2004 2535 513 Size: 552 939 531 3319 Fragments arranged by size: 3319 939 552 531 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4602 Fragments arranged by size: 4602 683 56 BtsI GCAGTG_nn' Cuts at: 2278 2857 3651 3671 2278 Size: 579 794 20 3948 Fragments arranged by size: 3948 794 579 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5341 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5306 DraI TTT'AAA Cuts at: 2645 2645 Size: 5341 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4348 809 Size: 507 3032 1802 Fragments arranged by size: 3032 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2226 2230 2532 Size: 54 1425 50 275 18 396 4 302 Cuts at: 2532 2788 3677 5306 8 Size: 256 889 1629 43 Fragments arranged by size: 1629 1425 889 396 302 275 256 54 50 43 18 4 EagI C'GGCC_G Cuts at: 1537 1812 2226 2788 1537 Size: 275 414 562 4090 Fragments arranged by size: 4090 562 414 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2573 3271 1353 Size: 1220 698 3423 Fragments arranged by size: 3423 1220 698 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3928 4302 4448 387 Size: 1064 138 2339 374 146 1280 Fragments arranged by size: 2339 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2316 2542 2316 Size: 226 5115 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2204 3404 4001 4788 5056 1239 Size: 393 572 1200 597 787 268 1524 Fragments arranged by size: 1524 1200 787 597 572 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2524 2524 Size: 5341 EcoO109I rG'GnC_Cy Cuts at: 2032 2560 2561 2032 Size: 528 1 4812 Fragments arranged by size: 4812 528 1 EcoRI G'AATT_C Cuts at: 2650 3028 2650 Size: 378 4963 FseI GG_CCGG'CC Cuts at: 2232 2232 Size: 5341 FspI TGC'GCA Cuts at: 3847 3847 Size: 5341 HaeII r_GCGC'y Cuts at: 1678 2116 2130 2143 4488 1678 Size: 438 14 13 2345 2531 Fragments arranged by size: 2531 2345 438 14 13 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3982 4014 4056 4088 1373 Size: 32 135 32 2410 32 42 32 2626 Fragments arranged by size: 2626 2410 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2164 2685 2931 669 Size: 988 281 226 521 246 3079 Fragments arranged by size: 3079 988 521 281 246 226 HindIII A'AGCT_T Cuts at: 748 748 Size: 5341 HpaI GTT'AAC Cuts at: 2931 2931 Size: 5341 KasI G'GCGC_C Cuts at: 2126 2126 Size: 5341 KpnI G_GTAC'C Cuts at: 2666 2666 Size: 5341 MfeI C'AATT_G Cuts at: 2940 2940 Size: 5341 MluI A'CGCG_T Cuts at: 5217 5217 Size: 5341 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2394 4455 4639 1305 Size: 1089 2061 184 2007 Fragments arranged by size: 2061 2007 1089 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2534 10 Size: 54 1425 343 702 2817 Fragments arranged by size: 2817 1425 702 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3299 3658 3817 518 Size: 648 2133 359 159 2042 Fragments arranged by size: 2133 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2383 2599 2757 3434 4582 4827 1606 Size: 777 216 158 677 1148 245 2120 Fragments arranged by size: 2120 1148 777 677 245 216 158 NaeI GCC'GGC Cuts at: 2230 2230 Size: 5341 NarI GG'CG_CC Cuts at: 2127 2127 Size: 5341 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3850 Fragments arranged by size: 3850 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5341 NgoMIV G'CCGG_C Cuts at: 2228 2228 Size: 5341 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5341 NotI GC'GGCC_GC Cuts at: 2788 2788 Size: 5341 NspI r_CATG'y Cuts at: 1132 1229 2699 4244 1132 Size: 97 1470 1545 2229 Fragments arranged by size: 2229 1545 1470 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5341 PciI A'CATG_T Cuts at: 4240 4240 Size: 5341 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5341 PmeI GTTT'AAAC Cuts at: 2645 2645 Size: 5341 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3410 3442 4923 4955 3410 Size: 32 1481 32 3796 Fragments arranged by size: 3796 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2032 2032 Size: 5341 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5341 PsiI TTA'TAA Cuts at: 2911 2911 Size: 5341 PspOMI G'GGCC_C Cuts at: 2017 2560 2017 Size: 543 4798 PspXI vC'TCGA_Gb Cuts at: 2500 2500 Size: 5341 PstI C_TGCA'G Cuts at: 830 2214 2693 830 Size: 1384 479 3478 Fragments arranged by size: 3478 1384 479 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5341 PvuII CAG'CTG Cuts at: 2383 2599 2383 Size: 216 5125 SacI G_AGCT'C Cuts at: 2318 2544 2318 Size: 226 5115 SalI G'TCGA_C Cuts at: 1936 2162 2683 1936 Size: 226 521 4594 Fragments arranged by size: 4594 521 226 SanDI GG'GwC_CC Cuts at: 2032 2032 Size: 5341 SapI GCTCTTCn'nnn_ Cuts at: 2573 2573 Size: 5341 SbfI CC_TGCA'GG Cuts at: 2693 2693 Size: 5341 ScaI AGT'ACT Cuts at: 3589 5301 3589 Size: 1712 3629 SfcI C'TryA_G Cuts at: 826 1045 2210 2689 3824 4505 4696 826 Size: 219 1165 479 1135 681 191 1471 Fragments arranged by size: 1471 1165 1135 681 479 219 191 SfoI GGC'GCC Cuts at: 2128 2128 Size: 5341 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5341 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5341 SmaI CCC'GGG Cuts at: 1872 2028 2668 1872 Size: 156 640 4545 Fragments arranged by size: 4545 640 156 SmlI C'TyrA_G Cuts at: 820 1017 1943 2500 3445 4346 4608 820 Size: 197 926 557 945 901 262 1553 Fragments arranged by size: 1553 945 926 901 557 262 197 SnaBI TAC'GTA Cuts at: 493 3132 493 Size: 2639 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5341 SphI G_CATG'C Cuts at: 1132 2699 1132 Size: 1567 3774 SspI AAT'ATT Cuts at: 5 52 3265 5 Size: 47 3213 2081 Fragments arranged by size: 3213 2081 47 StuI AGG'CCT Cuts at: 1517 2519 1517 Size: 1002 4339 StyI C'CwwG_G Cuts at: 513 1065 2004 2430 2493 2780 513 Size: 552 939 426 63 287 3074 Fragments arranged by size: 3074 939 552 426 287 63 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2270 3368 3385 3538 3723 4168 325 Size: 1255 690 1098 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1098 690 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3587 5299 96 Size: 274 80 33 51 1443 1610 1712 138 Fragments arranged by size: 1712 1610 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5301 5333 5301 Size: 32 5309 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5341 XbaI T'CTAG_A Cuts at: 2677 2677 Size: 5341 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2179 2179 Size: 5341 XhoI C'TCGA_G Cuts at: 1943 2500 1943 Size: 557 4784 XmaI C'CCGG_G Cuts at: 1870 2026 2666 1870 Size: 156 640 4545 Fragments arranged by size: 4545 640 156 XmnI GAAnn'nnTTC Cuts at: 3470 3470 Size: 5341 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2487 3149 276 Size: 53 83 186 936 953 662 2468 Fragments arranged by size: 2468 953 936 662 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoRV FalI FspAI NruI NsiI PacI PfoI PmlI PsrI RsrII SacII SexAI SfiI SrfI SwaI