################################## # NO CUT ENZYMES ( 179 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BamHI BanI BanII BbeI BbrPI BbvCI BbvI BcgI BciVI BcnI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsoBI Bsp1286I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HpaII KasI KpnI KspI MamI MfeI MflI MluI MroI MspA1I MspI MunI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I StuI SwaI TseI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AciI 346 GCGG CCGC(-3/-1) AcsI 919 AAATTT [GA]^AATT[CT] AlwI 610 GGATC GGATC(4/5) ApoI 919 AAATTT [GA]^AATT[CT] BalI 371 TGGCCA TGG^CCA BclI 107 TGATCA T^GATCA BsaI 509 GGTCTC GGTCTC(1/5) BsgI 782 GTGCAG GTGCAG(16/14) BsiYI 113 CCATGGTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 113 CCATGGTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 510 GTCTC GTCTC(1/5) BsmI 422 GCATTC GAATGC(1/-1) Bsp1407I 176 TGTACA T^GTACA BspMI 458 ACCTGC ACCTGC(4/8) BsrDI 349 GCAATG GCAATG(2/0) BsrGI 176 TGTACA T^GTACA BssKI 778 CCAGG ^CC[ACGT]GG BstNI 778 CCAGG CC^[AT]GG Cac8I 451 GCTGGC GC[ACGT]^[ACGT]GC DsaI 113 CCATGG C^C[GA][CT]GG EaeI 371 TGGCCA [CT]^GGCC[GA] EcoRII 778 CCAGG ^CC[AT]GG FbaI 107 TGATCA T^GATCA Fnu4HI 346 GCGGC GC^[ACGT]GC HincII 495 GTTAAC GT[CT]^[GA]AC HindII 495 GTTAAC GT[CT]^[GA]AC HindIII 701 AAGCTT A^AGCTT HpaI 495 GTTAAC GTT^AAC HphI 110 TCACC GGTGA(8/7) ItaI 346 GCGGC GC^[ACGT]GC MaeII 159 ACGT A^CGT MaeIII 926 GTCAC ^GT[ACGT]AC MluNI 371 TGGCCA TGG^CCA MscI 371 TGGCCA TGG^CCA MvaI 778 CCAGG CC^[AT]GG NcoI 113 CCATGG C^CATGG NdeI 375 CATATG CA^TATG NlaIV 439 GGAGCC GG[ACGT]^[ACGT]CC ScrFI 778 CCAGG CC^[ACGT]GG SexAI 777 ACCAGGT A^CC[AT]GGT SspBI 176 TGTACA T^GTACA TaqI 774 TCGA T^CGA Tsp45I 926 GTCAC ^GT[GC]AC TspRI 386 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 774 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AfaI 177 GTAC GT^AC 393 GTAC BbsI 69 GAAGAC GAAGAC(2/6) 161 GTCTTC BfaI 196 CTAG C^TAG 448 CTAG BpuAI 69 GAAGAC GAAGAC(2/6) 161 GTCTTC BsaJI 113 CCATGG C^C[ACGT][ACGT]GG 683 CCAAGG DpnII 108 GATC ^GATC 611 GATC EcoT14I 113 CCATGG C^C[AT][AT]GG 683 CCAAGG HaeIII 354 GGCC GG^CC 372 GGCC MaeI 196 CTAG C^TAG 448 CTAG MboI 108 GATC ^GATC 611 GATC MwoI 346 GCGGCAATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 442 GCCTACCTAGC NdeII 108 GATC ^GATC 611 GATC PstI 388 CTGCAG CTGCA^G 434 CTGCAG RsaI 177 GTAC GT^AC 393 GTAC Sau3AI 108 GATC ^GATC 611 GATC SfaNI 462 GCATC GCATC(5/9) 718 GCATC StyI 113 CCATGG C^C[AT][AT]GG 683 CCAAGG ######################################### # 3 OR MORE CUT ENZYMES ( 17 enzymes) # ######################################### AluI BstF5I DdeI EarI FokI HinfI Ksp632I MboII MnlI MseI MslI NlaIII SfcI SspI TfiI Tru9I Tsp509I