Restriction map for
pFN21AE6072
(Circular) (Six-base) MAPSORT of: pFN21AE6072.seq Check: 930 from: 1 to: 5668
pFN21AE6072 5668 bp
With 182 enzymes: SgfI *
December 8, 2014 13:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3478 278
Size: 53 83 186 936 1942 2468
Fragments arranged by size:
2468 1942 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3011 1937
Size: 1074 4594
Acc65I G'GTAC_C
Cuts at: 2989 2989
Size: 5668
AclI AA'CG_TT
Cuts at: 1563 3795 4168 1563
Size: 2232 373 3063
Fragments arranged by size:
3063 2232 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2093 3731 5115 5383 1632
Size: 461 1638 1384 268 1917
Fragments arranged by size:
1917 1638 1384 461 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5471
AflIII A'CryG_T
Cuts at: 1203 4567 5544 1203
Size: 3364 977 1327
Fragments arranged by size:
3364 1327 977
AgeI A'CCGG_T
Cuts at: 4499 4499
Size: 5668
AhdI GACnn_n'nnGTC
Cuts at: 4397 4397
Size: 5668
AleI CACnn'nnGTG
Cuts at: 1166 2120 1166
Size: 954 4714
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2408 1184
Size: 1224 4444
AlwNI CAG_nnn'CTG
Cuts at: 3411 4983 3411
Size: 1572 4096
ApaLI G'TGCA_C
Cuts at: 3725 4881 3725
Size: 1156 4512
ApoI r'AATT_y
Cuts at: 1493 2924 2977 3203 3355 5464 1493
Size: 1431 53 226 152 2109 1697
Fragments arranged by size:
2109 1697 1431 226 152 53
AseI AT'TA_AT
Cuts at: 160 1032 4222 160
Size: 872 3190 1606
Fragments arranged by size:
3190 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5668
AvaI C'yCGr_G
Cuts at: 1870 1943 2993 1870
Size: 73 1050 4545
Fragments arranged by size:
4545 1050 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2381 2414 2381
Size: 33 5635
BamHI G'GATC_C
Cuts at: 2998 3365 2998
Size: 367 5301
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2989 4444 618
Size: 325 217 627 1202 1455 1842
Fragments arranged by size:
1842 1455 1202 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2987 1391
Size: 1596 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2081 2159 928
Size: 971 182 78 4437
Fragments arranged by size:
4437 971 182 78
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3859 3893 1903
Size: 34 1922 34 3678
Fragments arranged by size:
3678 1922 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3560 4770 3560
Size: 1210 4458
BclI T'GATC_A
Cuts at: 1548 2112 1548
Size: 564 5104
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4279 136
Size: 107 122 71 631 605 2607 1525
Fragments arranged by size:
2607 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5663 5663
Size: 5668
BlpI GC'TnA_GC
Cuts at: 3085 3085
Size: 5668
Bme1580I G_kGCm'C
Cuts at: 1790 3729 4885 1790
Size: 1939 1156 2573
Fragments arranged by size:
2573 1939 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5668
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3399 4357 442
Size: 416 53 83 399 24 1982 958 1753
Fragments arranged by size:
1982 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5668
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2038 2038
Size: 5668
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4328 1239
Size: 3089 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3793 4658 4956 3793
Size: 865 298 4505
Fragments arranged by size:
4505 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2520 4331 882
Size: 617 1021 1811 2219
Fragments arranged by size:
2219 1811 1021 617
BsaAI yAC'GTr
Cuts at: 493 1204 3459 493
Size: 711 2255 2702
Fragments arranged by size:
2702 2255 711
BsaBI GATnn'nnATC
Cuts at: 5662 5662
Size: 5668
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3475 3857 4492 275
Size: 53 83 186 936 1942 382 635 1451
Fragments arranged by size:
1942 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4101 4499 4773 4920 5331 4101
Size: 398 274 147 411 4438
Fragments arranged by size:
4438 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5443
Fragments arranged by size:
5443 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5668
BseYI C'CCAG_C
Cuts at: 4871 5367 4871
Size: 496 5172
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2808 1163
Size: 573 1072 4023
Fragments arranged by size:
4023 1072 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3118 3879 4907 1540
Size: 275 186 1117 761 1028 2301
Fragments arranged by size:
2301 1117 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3729 3814 4885 3729
Size: 85 1071 4512
Fragments arranged by size:
4512 1071 85
BsmI GAATG_Cn'
Cuts at: 2427 3179 3272 2427
Size: 752 93 4823
Fragments arranged by size:
4823 752 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2987 3729 3814 4885 1391
Size: 399 1197 742 85 1071 2174
Fragments arranged by size:
2174 1197 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5331 5331
Size: 5668
BspHI T'CATG_A
Cuts at: 3557 5287 3557
Size: 1730 3938
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2472 3023 844
Size: 1628 551 3489
Fragments arranged by size:
3489 1628 551
BsrBI CCG'CTC
Cuts at: 1394 1823 3555 1394
Size: 429 1732 3507
Fragments arranged by size:
3507 1732 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2361 4163 4337 65
Size: 1183 1113 1802 174 1396
Fragments arranged by size:
1802 1396 1183 1113 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4312 4499 1700
Size: 110 2502 187 2869
Fragments arranged by size:
2869 2502 187 110
BsrGI T'GTAC_A
Cuts at: 96 2181 96
Size: 2085 3583
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5668
BssSI C'ACGA_G
Cuts at: 3728 4740 3728
Size: 1012 4656
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5668
BstBI TT'CG_AA
Cuts at: 4472 4472
Size: 5668
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5668
BstYI r'GATC_y
Cuts at: 1972 2998 3365 3750 3767 5219 5305 5317 5663
Size: 1026 367 385 17 1452 86 12 346
Cuts at: 5663 1972
Size: 1977
Fragments arranged by size:
1977 1452 1026 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2118 513
Size: 552 1053 4063
Fragments arranged by size:
4063 1053 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4929
Fragments arranged by size:
4929 683 56
BtsI GCAGTG_nn'
Cuts at: 2390 3184 3978 3998 2390
Size: 794 794 20 4060
Fragments arranged by size:
4060 794 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5668
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5633
DraI TTT'AAA
Cuts at: 2972 2972
Size: 5668
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4675 809
Size: 507 3359 1802
Fragments arranged by size:
3359 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2376 3115 4004
Size: 54 1425 50 275 18 546 739 889
Cuts at: 4004 5633 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 739 546 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3115 1537
Size: 275 1303 4090
Fragments arranged by size:
4090 1303 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2692 2771 2912 3598 1353
Size: 1339 79 141 686 3423
Fragments arranged by size:
3423 1339 686 141 79
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4255 4629 4775 387
Size: 1064 138 2666 374 146 1280
Fragments arranged by size:
2666 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2093 3731 4328 5115 5383 1239
Size: 393 461 1638 597 787 268 1524
Fragments arranged by size:
1638 1524 787 597 461 393 268
EcoRI G'AATT_C
Cuts at: 2977 3355 2977
Size: 378 5290
FspI TGC'GCA
Cuts at: 4174 4174
Size: 5668
HaeII r_GCGC'y
Cuts at: 1678 4815 1678
Size: 3137 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4309 4341 4383 4415 1373
Size: 32 135 32 2737 32 42 32 2626
Fragments arranged by size:
2737 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2502 3012 3258 669
Size: 988 281 564 510 246 3079
Fragments arranged by size:
3079 988 564 510 281 246
HindIII A'AGCT_T
Cuts at: 748 2706 748
Size: 1958 3710
HpaI GTT'AAC
Cuts at: 2502 3258 2502
Size: 756 4912
KpnI G_GTAC'C
Cuts at: 2993 2993
Size: 5668
MfeI C'AATT_G
Cuts at: 3267 3267
Size: 5668
MluI A'CGCG_T
Cuts at: 5544 5544
Size: 5668
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4782 4966 1305
Size: 3477 184 2007
Fragments arranged by size:
3477 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2378 10
Size: 54 1425 343 546 3300
Fragments arranged by size:
3300 1425 546 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2120 2177 2408 2432 3626 3985 4144
Size: 648 954 57 231 24 1194 359 159
Cuts at: 4144 518
Size: 2042
Fragments arranged by size:
2042 1194 954 648 359 231 159 57
24
MspA1I CmG'CkG
Cuts at: 1606 3084 3761 4909 5154 1606
Size: 1478 677 1148 245 2120
Fragments arranged by size:
2120 1478 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2118 513
Size: 552 1053 4063
Fragments arranged by size:
4063 1053 552
NdeI CA'TA_TG
Cuts at: 387 2381 387
Size: 1994 3674
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5668
NotI GC'GGCC_GC
Cuts at: 3115 3115
Size: 5668
NspI r_CATG'y
Cuts at: 1132 1229 3026 4571 1132
Size: 97 1797 1545 2229
Fragments arranged by size:
2229 1797 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5668
PciI A'CATG_T
Cuts at: 4567 4567
Size: 5668
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5668
PmeI GTTT'AAAC
Cuts at: 2972 2972
Size: 5668
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3737 3769 5250 5282 3737
Size: 32 1481 32 4123
Fragments arranged by size:
4123 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5668
PsiI TTA'TAA
Cuts at: 3238 3238
Size: 5668
PstI C_TGCA'G
Cuts at: 830 2397 2443 3020 830
Size: 1567 46 577 3478
Fragments arranged by size:
3478 1567 577 46
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5668
SalI G'TCGA_C
Cuts at: 1936 3010 1936
Size: 1074 4594
SbfI CC_TGCA'GG
Cuts at: 3020 3020
Size: 5668
ScaI AGT'ACT
Cuts at: 3916 5628 3916
Size: 1712 3956
SexAI A'CCwGG_T
Cuts at: 2782 2782
Size: 5668
SfcI C'TryA_G
Cuts at: 826 1045 2331 2393 2439 2634 3016 4151 4832
Size: 219 1286 62 46 195 382 1135 681
Cuts at: 4832 5023 826
Size: 191 1471
Fragments arranged by size:
1471 1286 1135 681 382 219 195 191
62 46
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5668
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5668
SmaI CCC'GGG
Cuts at: 1872 2995 1872
Size: 1123 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3772 4673 4935 820
Size: 197 926 1829 901 262 1553
Fragments arranged by size:
1829 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3459 493
Size: 2966 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5668
SphI G_CATG'C
Cuts at: 1132 3026 1132
Size: 1894 3774
SspI AAT'ATT
Cuts at: 5 52 2673 2748 2896 3592 5
Size: 47 2621 75 148 696 2081
Fragments arranged by size:
2621 2081 696 148 75 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5668
StyI C'CwwG_G
Cuts at: 513 1065 2118 2688 3107 513
Size: 552 1053 570 419 3074
Fragments arranged by size:
3074 1053 570 552 419
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3695 3712 3865 4050 4495 325
Size: 1255 2115 17 153 185 445 1498
Fragments arranged by size:
2115 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 3914 5626
Size: 274 80 33 51 1443 204 1733 1712
Cuts at: 5626 96
Size: 138
Fragments arranged by size:
1733 1712 1443 274 204 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5628 5660 5628
Size: 32 5636
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5668
XbaI T'CTAG_A
Cuts at: 3004 3004
Size: 5668
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5668
XmaI C'CCGG_G
Cuts at: 1870 2993 1870
Size: 1123 4545
XmnI GAAnn'nnTTC
Cuts at: 3797 3797
Size: 5668
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3476 276
Size: 53 83 186 936 1942 2468
Fragments arranged by size:
2468 1942 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SfiI SfoI SrfI SwaI XcmI