################################## # NO CUT ENZYMES ( 197 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaLI AscI Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BbvI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BsrI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRII EcoT22I FauI FbaI Fnu4HI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI ItaI KasI KpnI KspI MaeI MaeII MamI MflI MluI MluNI MroI MscI MspA1I MvaI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI ScrFI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TseI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 21 enzymes) # ################################### AciI 793 CCGC CCGC(-3/-1) AcsI 192 GAATTT [GA]^AATT[CT] ApaI 780 GGGCCC GGGCC^C ApoI 192 GAATTT [GA]^AATT[CT] BsaJI 186 CCTTGG C^C[ACGT][ACGT]GG Cac8I 795 GCAAGC GC[ACGT]^[ACGT]GC DpnII 361 GATC ^GATC DraII 780 GGGCCCT [GA]G^G[ACGT]CC[CT] EcoO109I 780 GGGCCCT [GA]G^G[ACGT]CC[CT] EcoT14I 186 CCTTGG C^C[AT][AT]GG HapII 426 CCGG C^CGG HpaII 426 CCGG C^CGG MboI 361 GATC ^GATC MfeI 314 CAATTG C^AATTG MspI 426 CCGG C^CGG MunI 314 CAATTG C^AATTG MwoI 346 GCAACAATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NdeII 361 GATC ^GATC Sau3AI 361 GATC ^GATC SfcI 867 CTACAG C^T[GA][CT]AG StyI 186 CCTTGG C^C[AT][AT]GG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AseI 432 ATTAAT AT^TAAT 580 ATTAAT AsnI 432 ATTAAT AT^TAAT 580 ATTAAT Asp700I 246 GAATAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 331 GAATGTTTTC BanII 725 GAGCCC G[GA]GC[CT]^C 780 GGGCCC BmyI 725 GAGCCC G[AGT]GC[ACT]^C 780 GGGCCC BsiYI 78 CCTGTTCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 113 CCATCATGGGG BslI 78 CCTGTTCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 113 CCATCATGGGG BsmI 286 GAATGC GAATGC(1/-1) 88 GCATTC Bsp1286I 725 GAGCCC G[AGT]GC[ACT]^C 780 GGGCCC BsrDI 20 CATTGC GCAATG(2/0) 309 CATTGC EcoRV 216 GATATC GAT^ATC 273 GATATC HaeIII 757 GGCC GG^CC 781 GGCC HinfI 123 GAATC G^A[ACGT]TC 571 GATTC HphI 110 TCACC GGTGA(8/7) 159 TCACC MslI 111 CACCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 903 CATAAAAATG NdeI 356 CATATG CA^TATG 375 CATATG PshBI 432 ATTAAT AT^TAAT 580 ATTAAT TfiI 123 GAATC G^A[AT]TC 571 GATTC XmnI 246 GAATAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 331 GAATGTTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 21 enzymes) # ######################################### AfaI AluI BstF5I Cfr13I DdeI EarI FokI Ksp632I MaeIII MboII MnlI MseI NlaIII NlaIV RsaI Sau96I SfaNI Tru9I Tsp45I Tsp509I TspRI