################################## # NO CUT ENZYMES ( 80 enzymes) # ################################## AatII Acc65I AccII AclI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI Asp718I AspEI AviII AvrII BbeI BbrPI BciVI BfrI BglI BlnI BsaAI BsiEI BsiWI BsmBI BspHI BsrFI BssHII BstUI Cfr10I CpoI DraI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRV FseI FspI HaeII HpaI KasI KpnI KspI MluI MvnI NaeI NarI NgoMIV NheI NotI NruI PacI PinAI PmaCI PmeI PmlI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I SwaI XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccIII 3437 TCCGGA T^CCGGA AcyI 2722 GGCGTC G[GA]^CG[CT]C AocI 4419 CCTGAGG CC^T[ACGT]AGG AspI 4081 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 852 CTCGAG C^[CT]CG[GA]G BclI 2378 TGATCA T^GATCA BglII 4097 AGATCT A^GATCT BsaBI 90 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 2722 GGCGTC G[GA]^CG[CT]C BsaWI 3437 TCCGGA [AT]^CCGG[AT] BseAI 3437 TCCGGA T^CCGGA BsmI 2340 GAATGC GAATGC(1/-1) BsoBI 852 CTCGAG C^[CT]CG[GA]G Bsp1407I 4793 TGTACA T^GTACA BspEI 3437 TCCGGA T^CCGGA BsrBI 37 GAGCGG CCGCTC(-3/-3) BsrGI 4793 TGTACA T^GTACA BstBI 5231 TTCGAA TT^CGAA Bsu36I 4419 CCTGAGG CC^T[ACGT]AGG DraIII 1565 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 4419 CCTGAGG CC^T[ACGT]AGG EcoRI 925 GAATTC G^AATTC FauI 2297 GCGGG CCCGC(4/6) FbaI 2378 TGATCA T^GATCA Hin1I 2722 GGCGTC G[GA]^CG[CT]C MamI 90 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 77 CAATTG C^AATTG MroI 3437 TCCGGA T^CCGGA MunI 77 CAATTG C^AATTG NdeI 1205 CATATG CA^TATG NspV 5231 TTCGAA TT^CGAA PaeR7I 852 CTCGAG C^TCGAG PflFI 4081 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 2112 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 1232 CTGCAG CTGCA^G SfuI 5231 TTCGAA TT^CGAA SspBI 4793 TGTACA T^GTACA Tth111I 4081 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 2679 CCAGGAGGAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 852 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AflIII 2900 ACATGT A^C[GA][CT]GT 4790 ACATGT AseI 997 ATTAAT AT^TAAT 3622 ATTAAT AsnI 997 ATTAAT AT^TAAT 3622 ATTAAT BamHI 2937 GGATCC G^GATCC 4018 GGATCC BbvCI 154 CCTCAGC CCTCAGC(-5/-2) 789 CCTCAGC BcgI 2084 GCAACTCTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 3839 GCAATTCACTCG BlpI 3201 GCTGAGC GC^T[ACGT]AGC 4668 GCTCAGC Bpu1102I 3201 GCTGAGC GC^T[ACGT]AGC 4668 GCTCAGC BsaI 2674 GAGACC GGTCTC(1/5) 4069 GGTCTC BspDI 2510 ATCGAT AT^CGAT 4582 ATCGAT BspLU11I 2900 ACATGT A^CATGT 4790 ACATGT CelII 3201 GCTGAGC GC^T[ACGT]AGC 4668 GCTCAGC ClaI 2510 ATCGAT AT^CGAT 4582 ATCGAT DrdI 595 GACTCCATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4297 GACTGTGTGGTC HgaI 4377 GACGC GACGC(5/10) 2723 GCGTC PpuMI 1703 AGGTCCT [GA]G^G[AT]CC[CT] 4844 AGGACCC PshBI 997 ATTAAT AT^TAAT 3622 ATTAAT SacI 214 GAGCTC GAGCT^C 2842 GAGCTC SspI 531 AATATT AAT^ATT 4925 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 119 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI AlwNI ApoI Asp700I AspHI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrDI BsrI BssKI BssSI Bst1107I BstAPI BstEII BstF5I BstNI BstPI BstXI BstYI BstZ17I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoO65I EcoRII EcoT14I EcoT22I Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NsiI NspI PflMI PleI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI