Restriction map for
pFN21AE6093
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# NO CUT ENZYMES ( 164 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AgeI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspHI AspI AvaI AvaII AviII
AvrII BalI BanI BbeI BbrPI
BbvCI BcgI BciVI BcnI BfaI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsoBI BspDI BspEI BsrBI
BssHII BssSI Bst1107I BstAPI BstBI
BstUI BstZ17I CelII CfoI ClaI
CpoI DraI DraII DraIII DrdI
DsaI EaeI EagI EclXI Eco47III
Eco52I EcoNI EcoO109I EcoRI EcoRV
EcoT22I FauI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HindIII
HpaI KasI KpnI KspI MaeI
MaeII MamI MfeI MluI MluNI
MroI MscI MseI MspA1I MunI
MvnI MwoI NaeI NarI NciI
NcoI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI TaqI Tru9I
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AflIII 224 ACATGT A^C[GA][CT]GT
AhdI 746 GACATAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 945 CCTGAGG CC^T[ACGT]AGG
AspEI 746 GACATAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 691 GGATCC G^GATCC
BanII 825 GAGCCC G[GA]GC[CT]^C
BbsI 754 GTCTTC GAAGAC(2/6)
BbvI 940 GCAGC GCAGC(8/12)
BclI 405 TGATCA T^GATCA
BglII 116 AGATCT A^GATCT
BmyI 825 GAGCCC G[AGT]GC[ACT]^C
BpuAI 754 GTCTTC GAAGAC(2/6)
BsaI 770 GGTCTC GGTCTC(1/5)
BsmI 376 GAATGC GAATGC(1/-1)
Bsp1286I 825 GAGCCC G[AGT]GC[ACT]^C
Bsp1407I 221 TGTACA T^GTACA
BspHI 449 TCATGA T^CATGA
BspLU11I 224 ACATGT A^CATGT
BspMI 18 GCAGGT ACCTGC(4/8)
BsrDI 177 CATTGC GCAATG(2/0)
BsrFI 200 ACCGGC [GA]^CCGG[CT]
BsrGI 221 TGTACA T^GTACA
BstEII 885 GGTGACC G^GT[ACGT]ACC
BstPI 885 GGTGACC G^GT[ACGT]ACC
BstXI 281 CCAAGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 945 CCTGAGG CC^T[ACGT]AGG
Cac8I 380 GCTTGC GC[ACGT]^[ACGT]GC
Cfr10I 200 ACCGGC [GA]^CCGG[CT]
Eam1105I 746 GACATAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 945 CCTGAGG CC^T[ACGT]AGG
EcoO65I 885 GGTGACC G^GT[ACGT]ACC
EcoT14I 87 CCAAGG C^C[AT][AT]GG
FbaI 405 TGATCA T^GATCA
Fnu4HI 940 GCAGC GC^[ACGT]GC
HapII 201 CCGG C^CGG
HincII 292 GTCAAC GT[CT]^[GA]AC
HindII 292 GTCAAC GT[CT]^[GA]AC
HpaII 201 CCGG C^CGG
HphI 885 GGTGA GGTGA(8/7)
ItaI 940 GCAGC GC^[ACGT]GC
MspI 201 CCGG C^CGG
NdeI 146 CATATG CA^TATG
NspI 224 ACATGT [GA]CATG^[CT]
PleI 833 GACTC GAGTC(4/5)
RcaI 449 TCATGA T^CATGA
SspBI 221 TGTACA T^GTACA
StyI 87 CCAAGG C^C[AT][AT]GG
TfiI 626 GAATC G^A[AT]TC
TseI 940 GCAGC G^C[AT]GC
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# TWO CUT ENZYMES ( 10 enzymes) #
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BpmI 340 CTCCAG CTGGAG(16/14)
919 CTCCAG
BsmFI 5 GTCCC GGGAC(10/14)
428 GTCCC
BstF5I 331 GGATG GGATG(2/0)
851 GGATG
BstYI 116 AGATCT [GA]^GATC[CT]
691 GGATCC
Cfr13I 553 GGCCC G^G[ACGT]CC
589 GGGCC
FokI 331 GGATG GGATG(9/13)
851 GGATG
HinfI 626 GAATC G^A[ACGT]TC
833 GACTC
MflI 116 AGATCT [GA]^GATC[CT]
691 GGATCC
Sau96I 553 GGCCC G^G[ACGT]CC
589 GGGCC
XhoII 116 AGATCT [GA]^GATC[CT]
691 GGATCC
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# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
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AcsI AfaI AluI AlwI ApoI
BsaJI BsiYI BslI BsmAI BsrI
BssKI BstNI DdeI DpnII EarI
EcoRII HaeIII Ksp632I MaeIII MboI
MboII MnlI MslI MvaI NdeII
NlaIII NlaIV RsaI Sau3AI ScrFI
SfaNI SfcI Tsp45I Tsp509I TspRI