################################## # NO CUT ENZYMES ( 188 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DdeI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FokI FseI FspI HapII HgaI Hin1I HincII HindII HindIII HinfI HpaI HpaII KasI KpnI KspI MaeI MaeIII MamI MfeI MluI MluNI MroI MscI MslI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI TfiI Tsp45I TspRI Tth111I Van91I XbaI XcmI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AfaI 71 GTAC GT^AC AlwI 149 GGATC GGATC(4/5) AlwNI 47 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 135 AGCGCT AGC^GCT AspHI 238 GAGCAC G[AT]GC[AT]^C AvaI 248 CTCGAG C^[CT]CG[GA]G BcgI 240 GCACTTGTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BmyI 238 GAGCAC G[AGT]GC[ACT]^C BseRI 95 GAGGAG GAGGAG(10/8) BsiHKAI 238 GAGCAC G[AT]GC[AT]^C BsiWI 70 CGTACG C^GTACG BsiYI 35 CCATGCAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 35 CCATGCAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsoBI 248 CTCGAG C^[CT]CG[GA]G Bsp1286I 238 GAGCAC G[AGT]GC[ACT]^C BsrI 328 CCAGT ACTGG(1/-1) BstYI 149 GGATCT [GA]^GATC[CT] Cac8I 15 GCTGGC GC[ACGT]^[ACGT]GC CfoI 136 GCGC GCG^C Cfr13I 81 GGCCC G^G[ACGT]CC DpnII 150 GATC ^GATC DraII 80 AGGCCCT [GA]G^G[ACGT]CC[CT] EaeI 43 CGGCCA [CT]^GGCC[GA] EarI 363 GAAGAG CTCTTC(1/4) Eco47III 135 AGCGCT AGC^GCT EcoO109I 80 AGGCCCT [GA]G^G[ACGT]CC[CT] HaeII 135 AGCGCT [GA]GCGC^[CT] HhaI 136 GCGC GCG^C HinP1I 136 GCGC G^CGC HphI 127 GGTGA GGTGA(8/7) Ksp632I 363 GAAGAG CTCTTC(1/4) MaeII 69 ACGT A^CGT MboI 150 GATC ^GATC MflI 149 GGATCT [GA]^GATC[CT] MseI 22 TTAA T^TAA MwoI 208 GCACCCACCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NdeII 150 GATC ^GATC PaeR7I 248 CTCGAG C^TCGAG PvuII 218 CAGCTG CAG^CTG RsaI 71 GTAC GT^AC Sau3AI 150 GATC ^GATC Sau96I 81 GGCCC G^G[ACGT]CC SmlI 248 CTCGAG C^T[CT][GA]AG SspI 274 AATATT AAT^ATT TaqI 249 TCGA T^CGA Tru9I 22 TTAA T^TAA Tsp509I 347 AATT ^AATT TthHB8I 249 TCGA T^CGA XhoI 248 CTCGAG C^TCGAG XhoII 149 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AluI 14 AGCT AG^CT 219 AGCT BsaJI 123 CCTGGG C^C[ACGT][ACGT]GG 296 CCCAGG BsmAI 49 GAGAC GTCTC(1/5) 246 GTCTC BssKI 123 CCTGG ^CC[ACGT]GG 297 CCAGG BstNI 123 CCTGG CC^[AT]GG 297 CCAGG EcoRII 123 CCTGG ^CC[AT]GG 297 CCAGG FauI 5 CCCGC CCCGC(4/6) 182 GCGGG HaeIII 44 GGCC GG^CC 81 GGCC MboII 363 GAAGA GAAGA(8/7) 369 GAAGA MspA1I 40 CAGCGG C[AC]G^C[GT]G 218 CAGCTG MvaI 123 CCTGG CC^[AT]GG 297 CCAGG NlaIII 36 CATG CATG^ 231 CATG ScrFI 123 CCTGG CC^[ACGT]GG 297 CCAGG ######################################### # 3 OR MORE CUT ENZYMES ( 7 enzymes) # ######################################### AciI BbvI BsmFI Fnu4HI ItaI MnlI TseI