################################## # NO CUT ENZYMES ( 106 enzymes) # ################################## AatII AccIII AclI AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I AspEI AspI AviII BbvCI BglII BlpI Bpu1102I BsaI BseAI BsiEI BsiWI BspDI BspEI BspLU11I BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FseI FspI HincII HindII HindIII HpaI Ksp632I KspI MfeI MluI MroI MunI NaeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI SexAI SfiI SfuI SgrAI SnaBI SphI Sse8387I StuI SwaI Tth111I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### Acc65I 1639 GGTACC G^GTACC AccI 554 GTCTAC GT^[AC][GT]AC AcyI 1061 GGCGCC G[GA]^CG[CT]C AflII 718 CTTAAG C^TTAAG AflIII 866 ACGTGT A^C[GA][CT]GT ApaI 1469 GGGCCC GGGCC^C Asp718I 1639 GGTACC G^GTACC AvrII 1605 CCTAGG C^CTAGG BamHI 2171 GGATCC G^GATCC BbeI 1061 GGCGCC GGCGC^C BbrPI 865 CACGTG CAC^GTG BciVI 2666 GTATCC GTATCC(6/5) BclI 1088 TGATCA T^GATCA BfrI 718 CTTAAG C^TTAAG BglI 1957 GCCAATCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 1605 CCTAGG C^CTAGG BsaBI 1159 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1061 GGCGCC G[GA]^CG[CT]C BsaWI 1625 ACCGGA [AT]^CCGG[AT] BsgI 355 GTGCAG GTGCAG(16/14) BsmBI 2685 GAGACG CGTCTC(1/5) BsmFI 44 GGGAC GGGAC(10/14) BsmI 1616 GCATTC GAATGC(1/-1) FbaI 1088 TGATCA T^GATCA HaeII 1061 GGCGCC [GA]GCGC^[CT] HgaI 2235 GACGC GACGC(5/10) Hin1I 1061 GGCGCC G[GA]^CG[CT]C KasI 1061 GGCGCC G^GCGCC KpnI 1639 GGTACC GGTAC^C MamI 1159 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NarI 1061 GGCGCC GG^CGCC NdeI 2374 CATATG CA^TATG NspI 286 GCATGT [GA]CATG^[CT] PmaCI 865 CACGTG CAC^GTG PmlI 865 CACGTG CAC^GTG SfoI 1061 GGCGCC GGC^GCC SmaI 28 CCCGGG CCC^GGG SpeI 1446 ACTAGT A^CTAGT XmaCI 28 CCCGGG C^CCGGG XmaI 28 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AccII 278 CGCG CG^CG 2199 CGCG AcsI 1218 GAATTC [GA]^AATT[CT] 2521 GAATTC AlwNI 704 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG 724 CAGATTCTG ApoI 1218 GAATTC [GA]^AATT[CT] 2521 GAATTC BalI 992 TGGCCA TGG^CCA 1286 TGGCCA BanI 1061 GGCGCC G^G[CT][GA]CC 1639 GGTACC BbsI 532 GAAGAC GAAGAC(2/6) 2419 GAAGAC BcgI 11 GCACACTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 368 GCAATGCTCTCG BpmI 579 CTCCAG CTGGAG(16/14) 1679 CTCCAG BpuAI 532 GAAGAC GAAGAC(2/6) 2419 GAAGAC BsaAI 865 CACGTG [CT]AC^GT[GA] 1561 TACGTG Bsp1407I 983 TGTACA T^GTACA 1337 TGTACA BspHI 1598 TCATGA T^CATGA 2123 TCATGA BsrDI 996 CATTGC GCAATG(2/0) 368 GCAATG BsrGI 983 TGTACA T^GTACA 1337 TGTACA BstUI 278 CGCG CG^CG 2199 CGCG CfoI 1062 GCGC GCG^C 2200 GCGC DsaI 893 CCATGG C^C[GA][CT]GG 1057 CCATGG EcoRI 1218 GAATTC G^AATTC 2521 GAATTC HhaI 1062 GCGC GCG^C 2200 GCGC HinP1I 1062 GCGC G^CGC 2200 GCGC MluNI 992 TGGCCA TGG^CCA 1286 TGGCCA MscI 992 TGGCCA TGG^CCA 1286 TGGCCA MslI 735 CATGGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1557 CACCTACGTG MvnI 278 CGCG CG^CG 2199 CGCG MwoI 1528 GCTGACCTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 1957 GCCAATCTGGC NcoI 893 CCATGG C^CATGG 1057 CCATGG PstI 904 CTGCAG CTGCA^G 1036 CTGCAG RcaI 1598 TCATGA T^CATGA 2123 TCATGA SacI 562 GAGCTC GAGCT^C 1850 GAGCTC ScaI 105 AGTACT AGT^ACT 752 AGTACT SspBI 983 TGTACA T^GTACA 1337 TGTACA SspI 589 AATATT AAT^ATT 2455 AATATT TaqI 377 TCGA T^CGA 1894 TCGA TthHB8I 377 TCGA T^CGA 1894 TCGA XcmI 294 CCAGCTGGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 609 CCAGCTGCCCTCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 76 enzymes) # ######################################### AciI AfaI AluI AlwI AspHI AvaI AvaII BanII BbvI BcnI BfaI BmyI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI NciI NdeII NlaIII NlaIV PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII