(Circular) (Six-base) MAPSORT of: pFN21AE0088.seq Check: 1512 from: 1 to: 7420 pFN21AE0088 7420 bp With 182 enzymes: SgfI * December 8, 2014 13:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5230 278 Size: 53 83 186 936 3694 2468 Fragments arranged by size: 3694 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2560 4763 1937 Size: 623 2203 4594 Fragments arranged by size: 4594 2203 623 Acc65I G'GTAC_C Cuts at: 3644 4741 3644 Size: 1097 6323 AclI AA'CG_TT Cuts at: 1563 5547 5920 1563 Size: 3984 373 3063 Fragments arranged by size: 3984 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2982 4631 5483 6867 7135 1632 Size: 1350 1649 852 1384 268 1917 Fragments arranged by size: 1917 1649 1384 1350 852 268 AflII C'TTAA_G Cuts at: 820 1017 2723 820 Size: 197 1706 5517 Fragments arranged by size: 5517 1706 197 AflIII A'CryG_T Cuts at: 1203 2871 6319 7296 1203 Size: 1668 3448 977 1327 Fragments arranged by size: 3448 1668 1327 977 AgeI A'CCGG_T Cuts at: 6251 6251 Size: 7420 AhdI GACnn_n'nnGTC Cuts at: 6149 6149 Size: 7420 AleI CACnn'nnGTG Cuts at: 1166 3566 1166 Size: 2400 5020 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7420 AlwNI CAG_nnn'CTG Cuts at: 2714 2734 5163 6735 2714 Size: 20 2429 1572 3399 Fragments arranged by size: 3399 2429 1572 20 ApaI G_GGCC'C Cuts at: 3478 3478 Size: 7420 ApaLI G'TGCA_C Cuts at: 5477 6633 5477 Size: 1156 6264 ApoI r'AATT_y Cuts at: 1493 3223 4526 4729 4955 5107 7216 1493 Size: 1730 1303 203 226 152 2109 1697 Fragments arranged by size: 2109 1730 1697 1303 226 203 152 AseI AT'TA_AT Cuts at: 160 1032 5974 160 Size: 872 4942 1606 Fragments arranged by size: 4942 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7420 AvaI C'yCGr_G Cuts at: 1870 1943 2033 2927 4418 4745 1870 Size: 73 90 894 1491 327 4545 Fragments arranged by size: 4545 1491 894 327 90 73 AvrII C'CTAG_G Cuts at: 3610 3610 Size: 7420 BamHI G'GATC_C Cuts at: 4176 4750 5117 4176 Size: 574 367 6479 Fragments arranged by size: 6479 574 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3066 3644 4741 6196 618 Size: 325 217 627 1279 578 1097 1455 1842 Fragments arranged by size: 1842 1455 1279 1097 627 578 325 217 BanII G_rGCy'C Cuts at: 1391 2040 2571 3478 3859 4739 1391 Size: 649 531 907 381 880 4072 Fragments arranged by size: 4072 907 880 649 531 381 BbeI G_GCGC'C Cuts at: 3070 3070 Size: 7420 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2544 4431 928 Size: 971 645 1887 3917 Fragments arranged by size: 3917 1887 971 645 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2005 2039 2362 2396 5611 5645 1903 Size: 34 68 34 323 34 3215 34 3678 Fragments arranged by size: 3678 3215 323 68 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4682 5312 6522 4682 Size: 630 1210 5580 Fragments arranged by size: 5580 1210 630 BclI T'GATC_A Cuts at: 1548 3093 1548 Size: 1545 5875 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3968 6031 136 Size: 107 122 71 631 605 2296 2063 1525 Fragments arranged by size: 2296 2063 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7415 7415 Size: 7420 BlpI GC'TnA_GC Cuts at: 4837 4837 Size: 7420 Bme1580I G_kGCm'C Cuts at: 1790 2065 3478 4447 5481 6637 1790 Size: 275 1413 969 1034 1156 2573 Fragments arranged by size: 2573 1413 1156 1034 969 275 BmgBI CAC'GTC Cuts at: 1318 3805 1318 Size: 2487 4933 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3132 4607 5151 Size: 416 53 83 399 24 1715 1475 544 Cuts at: 5151 6109 442 Size: 958 1753 Fragments arranged by size: 1753 1715 1475 958 544 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7420 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4713 4713 Size: 7420 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2569 3669 6080 1239 Size: 1330 1100 2411 2579 Fragments arranged by size: 2579 2411 1330 1100 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2636 3175 5545 6410 6708 2636 Size: 539 2370 865 298 3348 Fragments arranged by size: 3348 2370 865 539 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6083 882 Size: 617 4584 2219 Fragments arranged by size: 4584 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2872 3568 5211 493 Size: 711 1668 696 1643 2702 Fragments arranged by size: 2702 1668 1643 711 696 BsaBI GATnn'nnATC Cuts at: 3168 7414 3168 Size: 4246 3174 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3067 5227 5609 6244 Size: 53 83 186 936 1534 2160 382 635 Cuts at: 6244 275 Size: 1451 Fragments arranged by size: 2160 1534 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3630 5853 6251 6525 6672 7083 3630 Size: 2223 398 274 147 411 3967 Fragments arranged by size: 3967 2223 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2278 2308 1178 Size: 30 165 30 875 30 6290 Fragments arranged by size: 6290 875 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2012 2057 2335 3540 1186 Size: 826 45 278 1205 5066 Fragments arranged by size: 5066 1205 826 278 45 BseYI C'CCAG_C Cuts at: 2028 2058 3021 6623 7119 2028 Size: 30 963 3602 496 2329 Fragments arranged by size: 3602 2329 963 496 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2381 1163 Size: 573 645 6202 Fragments arranged by size: 6202 645 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4870 5631 6659 1540 Size: 275 186 2869 761 1028 2301 Fragments arranged by size: 2869 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2013 2571 3708 3859 4191 5481 5566 6637 2013 Size: 558 1137 151 332 1290 85 1071 2796 Fragments arranged by size: 2796 1290 1137 1071 558 332 151 85 BsmI GAATG_Cn' Cuts at: 3621 4931 5024 3621 Size: 1310 93 6017 Fragments arranged by size: 6017 1310 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4684 4684 Size: 7420 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2040 2065 2571 3478 3708 3859 Size: 399 223 27 25 506 907 230 151 Cuts at: 3859 4191 4447 4739 5481 5566 6637 1391 Size: 332 256 292 742 85 1071 2174 Fragments arranged by size: 2174 1071 907 742 506 399 332 292 256 230 223 151 85 27 25 BspEI T'CCGG_A Cuts at: 7083 7083 Size: 7420 BspHI T'CATG_A Cuts at: 3603 4128 5309 7039 3603 Size: 525 1181 1730 3984 Fragments arranged by size: 3984 1730 1181 525 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4775 844 Size: 3931 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5307 1394 Size: 429 3484 3507 Fragments arranged by size: 3507 3484 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2380 3000 5915 6089 65 Size: 1183 1132 620 2915 174 1396 Fragments arranged by size: 2915 1396 1183 1132 620 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6064 6251 1700 Size: 110 4254 187 2869 Fragments arranged by size: 4254 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2988 3342 96 Size: 2892 354 4174 Fragments arranged by size: 4174 2892 354 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7420 BssSI C'ACGA_G Cuts at: 5480 6492 5480 Size: 1012 6408 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7420 BstBI TT'CG_AA Cuts at: 6224 6224 Size: 7420 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7420 BstXI CCAn_nnnn'nTGG Cuts at: 3322 3693 4447 3322 Size: 371 754 6295 Fragments arranged by size: 6295 754 371 BstYI r'GATC_y Cuts at: 1972 2491 2800 4176 4750 5117 5502 5519 6971 Size: 519 309 1376 574 367 385 17 1452 Cuts at: 6971 7057 7069 7415 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1376 574 519 385 367 346 309 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2898 3062 513 Size: 552 939 894 164 4871 Fragments arranged by size: 4871 939 894 552 164 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6681 Fragments arranged by size: 6681 683 56 BtsI GCAGTG_nn' Cuts at: 3050 3549 4936 5730 5750 3050 Size: 499 1387 794 20 4720 Fragments arranged by size: 4720 1387 794 499 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7420 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4210 4245 529 Size: 35 3646 35 3704 Fragments arranged by size: 3704 3646 35 35 DraI TTT'AAA Cuts at: 4724 4724 Size: 7420 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6427 809 Size: 507 5111 1802 Fragments arranged by size: 5111 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2997 3291 3960 Size: 54 1425 50 275 18 1167 294 669 Cuts at: 3960 4867 5756 7385 8 Size: 907 889 1629 43 Fragments arranged by size: 1629 1425 1167 907 889 669 294 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4867 1537 Size: 275 3055 4090 Fragments arranged by size: 4090 3055 275 EarI CTCTTCn'nnn_ Cuts at: 1353 5350 1353 Size: 3997 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2600 6007 6381 6527 387 Size: 1064 138 1011 3407 374 146 1280 Fragments arranged by size: 3407 1280 1064 1011 374 146 138 EcoICRI GAG'CTC Cuts at: 2569 3857 2569 Size: 1288 6132 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2569 2982 3669 4631 5483 6080 6867 Size: 393 937 413 687 962 852 597 787 Cuts at: 6867 7135 1239 Size: 268 1524 Fragments arranged by size: 1524 962 937 852 787 687 597 413 393 268 EcoO109I rG'GnC_Cy Cuts at: 2914 3474 4273 2914 Size: 560 799 6061 Fragments arranged by size: 6061 799 560 EcoRI G'AATT_C Cuts at: 3223 4526 4729 5107 3223 Size: 1303 203 378 5536 Fragments arranged by size: 5536 1303 378 203 FspI TGC'GCA Cuts at: 5926 5926 Size: 7420 HaeII r_GCGC'y Cuts at: 1678 3070 6567 1678 Size: 1392 3497 2531 Fragments arranged by size: 3497 2531 1392 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2476 2508 2879 2911 6061 Size: 32 135 32 904 32 371 32 3150 Cuts at: 6061 6093 6135 6167 1373 Size: 32 42 32 2626 Fragments arranged by size: 3150 2626 904 371 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4764 5010 669 Size: 988 281 2826 246 3079 Fragments arranged by size: 3079 2826 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7420 HpaI GTT'AAC Cuts at: 5010 5010 Size: 7420 KasI G'GCGC_C Cuts at: 3066 3066 Size: 7420 KpnI G_GTAC'C Cuts at: 3648 4745 3648 Size: 1097 6323 MfeI C'AATT_G Cuts at: 5019 5019 Size: 7420 MluI A'CGCG_T Cuts at: 7296 7296 Size: 7420 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3252 3832 3892 6534 6718 1305 Size: 1947 580 60 2642 184 2007 Fragments arranged by size: 2642 2007 1947 580 184 60 MscI TGG'CCA Cuts at: 10 64 1489 1832 2999 3293 10 Size: 54 1425 343 1167 294 4137 Fragments arranged by size: 4137 1425 1167 343 294 54 MslI CAynn'nnrTG Cuts at: 518 1166 2744 3566 5378 5737 5896 518 Size: 648 1578 822 1812 359 159 2042 Fragments arranged by size: 2042 1812 1578 822 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2058 2302 2406 2617 2714 3025 3729 4836 Size: 452 244 104 211 97 311 704 1107 Cuts at: 4836 5513 6661 6906 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 1107 704 677 452 311 245 244 211 104 97 NarI GG'CG_CC Cuts at: 3067 3067 Size: 7420 NcoI C'CATG_G Cuts at: 513 1065 2004 2898 3062 513 Size: 552 939 894 164 4871 Fragments arranged by size: 4871 939 894 552 164 NdeI CA'TA_TG Cuts at: 387 4380 387 Size: 3993 3427 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7420 NotI GC'GGCC_GC Cuts at: 4867 4867 Size: 7420 NspI r_CATG'y Cuts at: 1132 1229 2295 4778 6323 1132 Size: 97 1066 2483 1545 2229 Fragments arranged by size: 2483 2229 1545 1066 97 PasI CC'CwG_GG Cuts at: 1348 4447 1348 Size: 3099 4321 PciI A'CATG_T Cuts at: 6319 6319 Size: 7420 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7420 PfoI T'CCnGG_A Cuts at: 2487 2487 Size: 7420 PmeI GTTT'AAAC Cuts at: 4724 4724 Size: 7420 PmlI CAC'GTG Cuts at: 2872 2872 Size: 7420 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4554 4586 5489 5521 7002 7034 4554 Size: 32 903 32 1481 32 4940 Fragments arranged by size: 4940 1481 903 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7420 PsiI TTA'TAA Cuts at: 4990 4990 Size: 7420 PspOMI G'GGCC_C Cuts at: 3474 3474 Size: 7420 PstI C_TGCA'G Cuts at: 830 2913 3045 4772 830 Size: 2083 132 1727 3478 Fragments arranged by size: 3478 2083 1727 132 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7420 PvuII CAG'CTG Cuts at: 2058 2302 2617 3025 3729 2058 Size: 244 315 408 704 5749 Fragments arranged by size: 5749 704 408 315 244 SacI G_AGCT'C Cuts at: 2571 3859 2571 Size: 1288 6132 SalI G'TCGA_C Cuts at: 1936 4762 1936 Size: 2826 4594 SbfI CC_TGCA'GG Cuts at: 4772 4772 Size: 7420 ScaI AGT'ACT Cuts at: 2112 2759 5668 7380 2112 Size: 647 2909 1712 2152 Fragments arranged by size: 2909 2152 1712 647 SfcI C'TryA_G Cuts at: 826 1045 2812 2909 3041 4768 5903 6584 6775 Size: 219 1767 97 132 1727 1135 681 191 Cuts at: 6775 826 Size: 1471 Fragments arranged by size: 1767 1727 1471 1135 681 219 191 132 97 SfoI GGC'GCC Cuts at: 3068 3068 Size: 7420 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7420 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7420 SmaI CCC'GGG Cuts at: 1872 2035 4747 1872 Size: 163 2712 4545 Fragments arranged by size: 4545 2712 163 SmlI C'TyrA_G Cuts at: 820 1017 1943 2651 2723 3154 5524 6425 6687 Size: 197 926 708 72 431 2370 901 262 Cuts at: 6687 820 Size: 1553 Fragments arranged by size: 2370 1553 926 901 708 431 262 197 72 SnaBI TAC'GTA Cuts at: 493 5211 493 Size: 4718 2702 SpeI A'CTAG_T Cuts at: 152 3451 152 Size: 3299 4121 SphI G_CATG'C Cuts at: 1132 4778 1132 Size: 3646 3774 SrfI GCCC'GGGC Cuts at: 2035 2035 Size: 7420 SspI AAT'ATT Cuts at: 5 52 2596 4462 5344 5 Size: 47 2544 1866 882 2081 Fragments arranged by size: 2544 2081 1866 882 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7420 StyI C'CwwG_G Cuts at: 513 1065 2004 2898 3062 3610 4859 513 Size: 552 939 894 164 548 1249 3074 Fragments arranged by size: 3074 1249 939 894 552 548 164 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2768 2935 3798 5447 5464 5617 5802 Size: 1255 1188 167 863 1649 17 153 185 Cuts at: 5802 6247 325 Size: 445 1498 Fragments arranged by size: 1649 1498 1255 1188 863 445 185 167 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2110 2757 2988 Size: 274 80 33 51 1443 133 647 231 Cuts at: 2988 3342 3733 5666 7378 96 Size: 354 391 1933 1712 138 Fragments arranged by size: 1933 1712 1443 647 391 354 274 231 138 133 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2278 2310 2861 2893 3002 3034 7380 7412 2278 Size: 32 551 32 109 32 4346 32 2286 Fragments arranged by size: 4346 2286 551 109 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7420 XbaI T'CTAG_A Cuts at: 4756 4756 Size: 7420 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2306 2621 2306 Size: 315 7105 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7420 XmaI C'CCGG_G Cuts at: 1870 2033 4745 1870 Size: 163 2712 4545 Fragments arranged by size: 4545 2712 163 XmnI GAAnn'nnTTC Cuts at: 5549 5549 Size: 7420 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5228 276 Size: 53 83 186 936 3694 2468 Fragments arranged by size: 3694 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SwaI