(Linear) (Six-base) MAPSORT of: KIEE0088.seq Check: 2416 from: 1 to: 2715 KIEE0088.orf With 182 enzymes: SgfI * December 5, 2014 12:55 .. AccI GT'mk_AC Cuts at: 0 555 2715 Size: 555 2160 Acc65I G'GTAC_C Cuts at: 0 1639 2715 Size: 1639 1076 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 977 2626 2715 Size: 977 1649 89 Fragments arranged by size: 1649 977 89 AflII C'TTAA_G Cuts at: 0 718 2715 Size: 718 1997 AflIII A'CryG_T Cuts at: 0 866 2715 Size: 866 1849 AleI CACnn'nnGTG Cuts at: 0 1561 2715 Size: 1561 1154 AlwNI CAG_nnn'CTG Cuts at: 0 709 729 2715 Size: 709 20 1986 Fragments arranged by size: 1986 709 20 ApaI G_GGCC'C Cuts at: 0 1473 2715 Size: 1473 1242 ApoI r'AATT_y Cuts at: 0 1218 2521 2715 Size: 1218 1303 194 Fragments arranged by size: 1303 1218 194 AvaI C'yCGr_G Cuts at: 0 28 922 2413 2715 Size: 28 894 1491 302 Fragments arranged by size: 1491 894 302 28 AvrII C'CTAG_G Cuts at: 0 1605 2715 Size: 1605 1110 BamHI G'GATC_C Cuts at: 0 2171 2715 Size: 2171 544 BanI G'GyrC_C Cuts at: 0 1061 1639 2715 Size: 1061 578 1076 Fragments arranged by size: 1076 1061 578 BanII G_rGCy'C Cuts at: 0 35 566 1473 1854 2715 Size: 35 531 907 381 861 Fragments arranged by size: 907 861 531 381 35 BbeI G_GCGC'C Cuts at: 0 1065 2715 Size: 1065 1650 BbsI GAAGACnn'nnnn_ Cuts at: 0 539 2426 2715 Size: 539 1887 289 Fragments arranged by size: 1887 539 289 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 34 357 391 2715 Size: 34 323 34 2324 Fragments arranged by size: 2324 323 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 2677 2715 Size: 2677 38 BclI T'GATC_A Cuts at: 0 1088 2715 Size: 1088 1627 BglI GCCn_nnn'nGGC Cuts at: 0 1963 2715 Size: 1963 752 Bme1580I G_kGCm'C Cuts at: 0 60 1473 2442 2715 Size: 60 1413 969 273 Fragments arranged by size: 1413 969 273 60 BmgBI CAC'GTC Cuts at: 0 1800 2715 Size: 1800 915 BmrI ACTGGGnnnn_n' Cuts at: 0 1127 2602 2715 Size: 1127 1475 113 Fragments arranged by size: 1475 1127 113 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2708 2715 Size: 2708 7 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 564 1664 2715 Size: 564 1100 1051 Fragments arranged by size: 1100 1051 564 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 631 1170 2715 Size: 631 539 1545 Fragments arranged by size: 1545 631 539 BsaAI yAC'GTr Cuts at: 0 867 1563 2715 Size: 867 696 1152 Fragments arranged by size: 1152 867 696 BsaBI GATnn'nnATC Cuts at: 0 1163 2715 Size: 1163 1552 BsaHI Gr'CG_yC Cuts at: 0 1062 2715 Size: 1062 1653 BsaWI w'CCGG_w Cuts at: 0 1625 2715 Size: 1625 1090 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 273 303 2715 Size: 273 30 2412 Fragments arranged by size: 2412 273 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 7 52 330 1535 2715 Size: 7 45 278 1205 1180 Fragments arranged by size: 1205 1180 278 45 7 BseYI C'CCAG_C Cuts at: 0 23 53 1016 2715 Size: 23 30 963 1699 Fragments arranged by size: 1699 963 30 23 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 376 2715 Size: 376 2339 BsiHKAI G_wGCw'C Cuts at: 0 8 566 1703 1854 2186 2715 Size: 8 558 1137 151 332 529 Fragments arranged by size: 1137 558 529 332 151 8 BsmI GAATG_Cn' Cuts at: 0 1616 2715 Size: 1616 1099 BsmBI CGTCTCn'nnnn_ Cuts at: 0 2679 2715 Size: 2679 36 Bsp1286I G_dGCh'C Cuts at: 0 8 35 60 566 1473 1703 1854 2186 Size: 8 27 25 506 907 230 151 332 Cuts at: 2186 2442 2715 Size: 256 273 Fragments arranged by size: 907 506 332 273 256 230 151 27 25 8 BspHI T'CATG_A Cuts at: 0 1598 2123 2715 Size: 1598 525 592 Fragments arranged by size: 1598 592 525 BsrDI GCAATG_nn' Cuts at: 0 375 995 2715 Size: 375 620 1720 Fragments arranged by size: 1720 620 375 BsrGI T'GTAC_A Cuts at: 0 983 1337 2715 Size: 983 354 1378 Fragments arranged by size: 1378 983 354 BstXI CCAn_nnnn'nTGG Cuts at: 0 1317 1688 2442 2715 Size: 1317 371 754 273 Fragments arranged by size: 1317 754 371 273 BstYI r'GATC_y Cuts at: 0 486 795 2171 2715 Size: 486 309 1376 544 Fragments arranged by size: 1376 544 486 309 BtgI C'CryG_G Cuts at: 0 893 1057 2715 Size: 893 164 1658 Fragments arranged by size: 1658 893 164 BtsI GCAGTG_nn' Cuts at: 0 1045 1544 2715 Size: 1045 499 1171 Fragments arranged by size: 1171 1045 499 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 2205 2240 2715 Size: 2205 35 475 Fragments arranged by size: 2205 475 35 EaeI y'GGCC_r Cuts at: 0 992 1286 1955 2715 Size: 992 294 669 760 Fragments arranged by size: 992 760 669 294 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 595 2715 Size: 595 2120 EcoICRI GAG'CTC Cuts at: 0 564 1852 2715 Size: 564 1288 863 Fragments arranged by size: 1288 863 564 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 564 977 1664 2626 2715 Size: 564 413 687 962 89 Fragments arranged by size: 962 687 564 413 89 EcoO109I rG'GnC_Cy Cuts at: 0 909 1469 2268 2715 Size: 909 560 799 447 Fragments arranged by size: 909 799 560 447 EcoRI G'AATT_C Cuts at: 0 1218 2521 2715 Size: 1218 1303 194 Fragments arranged by size: 1303 1218 194 HaeII r_GCGC'y Cuts at: 0 1065 2715 Size: 1065 1650 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 471 503 874 906 2715 Size: 471 32 371 32 1809 Fragments arranged by size: 1809 471 371 32 32 KasI G'GCGC_C Cuts at: 0 1061 2715 Size: 1061 1654 KpnI G_GTAC'C Cuts at: 0 1643 2715 Size: 1643 1072 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1247 1827 1887 2715 Size: 1247 580 60 828 Fragments arranged by size: 1247 828 580 60 MscI TGG'CCA Cuts at: 0 994 1288 2715 Size: 994 294 1427 Fragments arranged by size: 1427 994 294 MslI CAynn'nnrTG Cuts at: 0 739 1561 2715 Size: 739 822 1154 Fragments arranged by size: 1154 822 739 MspA1I CmG'CkG Cuts at: 0 53 297 401 612 709 1020 1724 2715 Size: 53 244 104 211 97 311 704 991 Fragments arranged by size: 991 704 311 244 211 104 97 53 NarI GG'CG_CC Cuts at: 0 1062 2715 Size: 1062 1653 NcoI C'CATG_G Cuts at: 0 893 1057 2715 Size: 893 164 1658 Fragments arranged by size: 1658 893 164 NdeI CA'TA_TG Cuts at: 0 2375 2715 Size: 2375 340 NspI r_CATG'y Cuts at: 0 290 2715 Size: 290 2425 PasI CC'CwG_GG Cuts at: 0 2442 2715 Size: 2442 273 PfoI T'CCnGG_A Cuts at: 0 482 2715 Size: 482 2233 PmlI CAC'GTG Cuts at: 0 867 2715 Size: 867 1848 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2549 2581 2715 Size: 2549 32 134 Fragments arranged by size: 2549 134 32 PspOMI G'GGCC_C Cuts at: 0 1469 2715 Size: 1469 1246 PstI C_TGCA'G Cuts at: 0 908 1040 2715 Size: 908 132 1675 Fragments arranged by size: 1675 908 132 PvuII CAG'CTG Cuts at: 0 53 297 612 1020 1724 2715 Size: 53 244 315 408 704 991 Fragments arranged by size: 991 704 408 315 244 53 SacI G_AGCT'C Cuts at: 0 566 1854 2715 Size: 566 1288 861 Fragments arranged by size: 1288 861 566 ScaI AGT'ACT Cuts at: 0 107 754 2715 Size: 107 647 1961 Fragments arranged by size: 1961 647 107 SfcI C'TryA_G Cuts at: 0 807 904 1036 2715 Size: 807 97 132 1679 Fragments arranged by size: 1679 807 132 97 SfoI GGC'GCC Cuts at: 0 1063 2715 Size: 1063 1652 SmaI CCC'GGG Cuts at: 0 30 2715 Size: 30 2685 SmlI C'TyrA_G Cuts at: 0 646 718 1149 2715 Size: 646 72 431 1566 Fragments arranged by size: 1566 646 431 72 SpeI A'CTAG_T Cuts at: 0 1446 2715 Size: 1446 1269 SrfI GCCC'GGGC Cuts at: 0 30 2715 Size: 30 2685 SspI AAT'ATT Cuts at: 0 591 2457 2715 Size: 591 1866 258 Fragments arranged by size: 1866 591 258 StyI C'CwwG_G Cuts at: 0 893 1057 1605 2715 Size: 893 164 548 1110 Fragments arranged by size: 1110 893 548 164 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 763 930 1793 2715 Size: 763 167 863 922 Fragments arranged by size: 922 863 763 167 TatI w'GTAC_w Cuts at: 0 105 752 983 1337 1728 2715 Size: 105 647 231 354 391 987 Fragments arranged by size: 987 647 391 354 231 105 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 273 305 856 888 997 1029 2715 Size: 273 32 551 32 109 32 1686 Fragments arranged by size: 1686 551 273 109 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 301 616 2715 Size: 301 315 2099 Fragments arranged by size: 2099 315 301 XmaI C'CCGG_G Cuts at: 0 28 2715 Size: 28 2687 Enzymes that do cut: AccI Acc65I AcuI AflII AflIII AleI AlwNI ApaI ApoI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BsrDI BsrGI BstXI BstYI BtgI BtsI CspCI EaeI EciI EcoICRI Eco57MI EcoO109I EcoRI HaeII Hin4I KasI KpnI MmeI MscI MslI MspA1I NarI NcoI NdeI NspI PasI PfoI PmlI PpiI PspOMI PstI PvuII SacI ScaI SfcI SfoI SmaI SmlI SpeI SrfI SspI StyI TaqII TatI TstI XcmI XmaI Enzymes that do not cut: AarI AatII AclI AfeI AgeI AhdI AlfI AloI ApaLI AscI AseI AsiSI BaeI BbvCI BfrBI BglII BlpI BmtI Bpu10I BsaI BsiEI BsiWI BspEI BspMI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI DraI DraIII DrdI EagI EarI EcoNI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI MfeI MluI NaeI NgoMIV NheI NotI NruI NsiI PacI PciI PflMI PmeI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI SexAI SfiI SgfI SgrAI SnaBI SphI StuI SwaI Tth111I XbaI XhoI XmnI ZraI