(Circular) (Six-base) MAPSORT of: pFN21AB9686.seq Check: 6147 from: 1 to: 5302 pFN21AB9686 5302 bp With 182 enzymes: SgfI * December 8, 2014 13:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3112 278 Size: 53 83 186 936 1576 2468 Fragments arranged by size: 2468 1576 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2645 1937 Size: 708 4594 Acc65I G'GTAC_C Cuts at: 2623 2623 Size: 5302 AclI AA'CG_TT Cuts at: 1563 3429 3802 1563 Size: 1866 373 3063 Fragments arranged by size: 3063 1866 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3365 4749 5017 1632 Size: 1733 1384 268 1917 Fragments arranged by size: 1917 1733 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5105 AflIII A'CryG_T Cuts at: 1203 2100 4201 5178 1203 Size: 897 2101 977 1327 Fragments arranged by size: 2101 1327 977 897 AgeI A'CCGG_T Cuts at: 4133 4133 Size: 5302 AhdI GACnn_n'nnGTC Cuts at: 4031 4031 Size: 5302 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5302 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5302 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2099 2131 2099 Size: 32 5270 AlwNI CAG_nnn'CTG Cuts at: 3045 4617 3045 Size: 1572 3730 ApaI G_GGCC'C Cuts at: 2135 2162 2204 2135 Size: 27 42 5233 Fragments arranged by size: 5233 42 27 ApaLI G'TGCA_C Cuts at: 3359 4515 3359 Size: 1156 4146 ApoI r'AATT_y Cuts at: 1493 2611 2837 2989 5098 1493 Size: 1118 226 152 2109 1697 Fragments arranged by size: 2109 1697 1118 226 152 AseI AT'TA_AT Cuts at: 160 1032 3856 160 Size: 872 2824 1606 Fragments arranged by size: 2824 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5302 AvaI C'yCGr_G Cuts at: 1870 1943 2155 2162 2627 1870 Size: 73 212 7 465 4545 Fragments arranged by size: 4545 465 212 73 7 BamHI G'GATC_C Cuts at: 2632 2999 2632 Size: 367 4935 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2450 2623 4078 618 Size: 325 217 627 663 173 1455 1842 Fragments arranged by size: 1842 1455 663 627 325 217 173 BanII G_rGCy'C Cuts at: 1391 2135 2162 2204 2621 1391 Size: 744 27 42 417 4072 Fragments arranged by size: 4072 744 417 42 27 BbeI G_GCGC'C Cuts at: 2454 2454 Size: 5302 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4331 BbvCI CC'TCA_GC Cuts at: 2510 2510 Size: 5302 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3493 3527 1903 Size: 34 1556 34 3678 Fragments arranged by size: 3678 1556 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3194 4404 3194 Size: 1210 4092 BclI T'GATC_A Cuts at: 1548 1548 Size: 5302 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2166 2282 3913 Size: 107 122 71 631 605 494 116 1631 Cuts at: 3913 136 Size: 1525 Fragments arranged by size: 1631 1525 631 605 494 122 116 107 71 BglII A'GATC_T Cuts at: 5297 5297 Size: 5302 BlpI GC'TnA_GC Cuts at: 2066 2719 2066 Size: 653 4649 Bme1580I G_kGCm'C Cuts at: 1790 2135 2162 2204 3363 4519 1790 Size: 345 27 42 1159 1156 2573 Fragments arranged by size: 2573 1159 1156 345 42 27 BmgBI CAC'GTC Cuts at: 1318 2056 1318 Size: 738 4564 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3033 3991 442 Size: 416 53 83 399 24 1616 958 1753 Fragments arranged by size: 1753 1616 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5302 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3962 1239 Size: 2723 2579 Bpu10I CC'TnA_GC Cuts at: 2510 2510 Size: 5302 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3427 4292 4590 3427 Size: 865 298 4139 Fragments arranged by size: 4139 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3965 882 Size: 617 2466 2219 Fragments arranged by size: 2466 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2041 3093 493 Size: 711 837 1052 2702 Fragments arranged by size: 2702 1052 837 711 BsaBI GATnn'nnATC Cuts at: 5296 5296 Size: 5302 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2354 2451 3109 3491 Size: 53 83 186 936 821 97 658 382 Cuts at: 3491 4126 275 Size: 635 1451 Fragments arranged by size: 1451 936 821 658 635 382 186 97 83 53 BsaWI w'CCGG_w Cuts at: 3735 4133 4407 4554 4965 3735 Size: 398 274 147 411 4072 Fragments arranged by size: 4072 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5077 Fragments arranged by size: 5077 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2093 1186 Size: 907 4395 BseYI C'CCAG_C Cuts at: 2392 4505 5001 2392 Size: 2113 496 2693 Fragments arranged by size: 2693 2113 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2482 1163 Size: 573 746 3983 Fragments arranged by size: 3983 746 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2406 2479 2752 3513 4541 1540 Size: 275 186 405 73 273 761 1028 2301 Fragments arranged by size: 2301 1028 761 405 275 273 186 73 BsiHKAI G_wGCw'C Cuts at: 3363 3448 4519 3363 Size: 85 1071 4146 Fragments arranged by size: 4146 1071 85 BsmI GAATG_Cn' Cuts at: 2813 2906 2813 Size: 93 5209 BsmBI CGTCTCn'nnnn_ Cuts at: 2333 2333 Size: 5302 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2135 2162 2204 2621 3363 3448 4519 Size: 399 345 27 42 417 742 85 1071 Cuts at: 4519 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 417 399 345 85 42 27 BspEI T'CCGG_A Cuts at: 4965 4965 Size: 5302 BspHI T'CATG_A Cuts at: 3191 4921 3191 Size: 1730 3572 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2657 844 Size: 1813 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2248 3189 1394 Size: 429 425 941 3507 Fragments arranged by size: 3507 941 429 425 BsrDI GCAATG_nn' Cuts at: 65 1248 3797 3971 65 Size: 1183 2549 174 1396 Fragments arranged by size: 2549 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2311 3946 4133 1700 Size: 110 501 1635 187 2869 Fragments arranged by size: 2869 1635 501 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5302 BssHII G'CGCG_C Cuts at: 1925 2034 1925 Size: 109 5193 BssSI C'ACGA_G Cuts at: 3362 4374 3362 Size: 1012 4290 BstAPI GCAn_nnn'nTGC Cuts at: 794 2505 794 Size: 1711 3591 BstBI TT'CG_AA Cuts at: 4106 4106 Size: 5302 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5302 BstYI r'GATC_y Cuts at: 1972 2632 2999 3384 3401 4853 4939 4951 5297 Size: 660 367 385 17 1452 86 12 346 Cuts at: 5297 1972 Size: 1977 Fragments arranged by size: 1977 1452 660 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2205 2433 2594 513 Size: 552 939 201 228 161 3221 Fragments arranged by size: 3221 939 552 228 201 161 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2350 487 Size: 683 56 1124 3439 Fragments arranged by size: 3439 1124 683 56 BtsI GCAGTG_nn' Cuts at: 2446 2818 3612 3632 2446 Size: 372 794 20 4116 Fragments arranged by size: 4116 794 372 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5302 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2512 2547 529 Size: 35 1948 35 3284 Fragments arranged by size: 3284 1948 35 35 DraI TTT'AAA Cuts at: 2606 2606 Size: 5302 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4309 809 Size: 507 2993 1802 Fragments arranged by size: 2993 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2232 2376 2403 Size: 54 1425 50 275 18 402 144 27 Cuts at: 2403 2476 2533 2749 3638 5267 8 Size: 73 57 216 889 1629 43 Fragments arranged by size: 1629 1425 889 402 275 216 144 73 57 54 50 43 27 18 EagI C'GGCC_G Cuts at: 1537 1812 2403 2476 2749 1537 Size: 275 591 73 273 4090 Fragments arranged by size: 4090 591 275 273 73 EarI CTCTTCn'nnn_ Cuts at: 1353 3232 1353 Size: 1879 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3889 4263 4409 387 Size: 1064 138 2300 374 146 1280 Fragments arranged by size: 2300 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3365 3962 4749 5017 1239 Size: 393 1733 597 787 268 1524 Fragments arranged by size: 1733 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2131 2159 2201 2131 Size: 28 42 5232 Fragments arranged by size: 5232 42 28 EcoRI G'AATT_C Cuts at: 2611 2989 2611 Size: 378 4924 FspI TGC'GCA Cuts at: 2291 3808 2291 Size: 1517 3785 HaeII r_GCGC'y Cuts at: 1678 2288 2454 4449 1678 Size: 610 166 1995 2531 Fragments arranged by size: 2531 1995 610 166 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3943 3975 4017 4049 1373 Size: 32 135 32 2371 32 42 32 2626 Fragments arranged by size: 2626 2371 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2646 2892 669 Size: 988 281 708 246 3079 Fragments arranged by size: 3079 988 708 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5302 HpaI GTT'AAC Cuts at: 2892 2892 Size: 5302 KasI G'GCGC_C Cuts at: 2450 2450 Size: 5302 KpnI G_GTAC'C Cuts at: 2627 2627 Size: 5302 MfeI C'AATT_G Cuts at: 2901 2901 Size: 5302 MluI A'CGCG_T Cuts at: 2100 5178 2100 Size: 3078 2224 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2085 4416 4600 1305 Size: 780 2331 184 2007 Fragments arranged by size: 2331 2007 780 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2378 10 Size: 54 1425 343 546 2934 Fragments arranged by size: 2934 1425 546 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3260 3619 3778 518 Size: 648 2094 359 159 2042 Fragments arranged by size: 2094 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2224 2435 2556 2718 3395 4543 4788 1606 Size: 618 211 121 162 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 618 245 211 162 121 NarI GG'CG_CC Cuts at: 2451 2451 Size: 5302 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3811 Fragments arranged by size: 3811 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5302 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5302 NotI GC'GGCC_GC Cuts at: 2476 2749 2476 Size: 273 5029 NspI r_CATG'y Cuts at: 1132 1229 2660 4205 1132 Size: 97 1431 1545 2229 Fragments arranged by size: 2229 1545 1431 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5302 PciI A'CATG_T Cuts at: 4201 4201 Size: 5302 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5302 PmeI GTTT'AAAC Cuts at: 2606 2606 Size: 5302 PmlI CAC'GTG Cuts at: 2041 2041 Size: 5302 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3371 3403 4884 4916 3371 Size: 32 1481 32 3757 Fragments arranged by size: 3757 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5302 PsiI TTA'TAA Cuts at: 2872 2872 Size: 5302 PspOMI G'GGCC_C Cuts at: 2131 2158 2200 2131 Size: 27 42 5233 Fragments arranged by size: 5233 42 27 PstI C_TGCA'G Cuts at: 830 2654 830 Size: 1824 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5302 PvuII CAG'CTG Cuts at: 2556 2556 Size: 5302 SacII CC_GC'GG Cuts at: 2436 2436 Size: 5302 SalI G'TCGA_C Cuts at: 1936 2644 1936 Size: 708 4594 SbfI CC_TGCA'GG Cuts at: 2654 2654 Size: 5302 ScaI AGT'ACT Cuts at: 3550 5262 3550 Size: 1712 3590 SfcI C'TryA_G Cuts at: 826 1045 2650 3785 4466 4657 826 Size: 219 1605 1135 681 191 1471 Fragments arranged by size: 1605 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2452 2452 Size: 5302 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5302 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5302 SmaI CCC'GGG Cuts at: 1872 2157 2164 2629 1872 Size: 285 7 465 4545 Fragments arranged by size: 4545 465 285 7 SmlI C'TyrA_G Cuts at: 820 1017 1943 3406 4307 4569 820 Size: 197 926 1463 901 262 1553 Fragments arranged by size: 1553 1463 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3093 493 Size: 2600 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5302 SphI G_CATG'C Cuts at: 1132 2660 1132 Size: 1528 3774 SrfI GCCC'GGGC Cuts at: 2157 2157 Size: 5302 SspI AAT'ATT Cuts at: 5 52 3226 5 Size: 47 3174 2081 Fragments arranged by size: 3174 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5302 StyI C'CwwG_G Cuts at: 513 1065 2004 2445 2741 513 Size: 552 939 441 296 3074 Fragments arranged by size: 3074 939 552 441 296 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3329 3346 3499 3684 4129 325 Size: 1255 1749 17 153 185 445 1498 Fragments arranged by size: 1749 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3548 5260 96 Size: 274 80 33 51 1443 1571 1712 138 Fragments arranged by size: 1712 1571 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5262 5294 5262 Size: 32 5270 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5302 XbaI T'CTAG_A Cuts at: 2638 2638 Size: 5302 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2311 2311 Size: 5302 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5302 XmaI C'CCGG_G Cuts at: 1870 2155 2162 2627 1870 Size: 285 7 465 4545 Fragments arranged by size: 4545 465 285 7 XmnI GAAnn'nnTTC Cuts at: 3431 3431 Size: 5302 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3110 276 Size: 53 83 186 936 1576 2468 Fragments arranged by size: 2468 1576 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PpuMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SwaI