################################## # NO CUT ENZYMES ( 92 enzymes) # ################################## AatII Acc65I AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII BbeI BbrPI BbvCI BlpI Bpu1102I BsiEI BsiWI BsmI BsoBI BspDI BspHI BsrBI Bst1107I BstAPI BstBI BstZ17I Bsu36I CelII ClaI CpoI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRI EcoT22I FseI FspI HaeII HpaI KasI KpnI MluI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI PvuI RcaI RsrII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I StuI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AccIII 2071 TCCGGA T^CCGGA AclI 858 AACGTT AA^CGTT AflII 732 CTTAAG C^TTAAG Asp700I 1637 GAAATAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvrII 1509 CCTAGG C^CTAGG BalI 155 TGGCCA TGG^CCA BamHI 645 GGATCC G^GATCC BcgI 1170 CGACTCCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 574 GGATAC GTATCC(6/5) BclI 1314 TGATCA T^GATCA BfrI 732 CTTAAG C^TTAAG BglI 1427 GCCTTCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1519 AGATCT A^GATCT BlnI 1509 CCTAGG C^CTAGG BsaAI 524 TACGTG [CT]AC^GT[GA] BsaBI 1299 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 2071 TCCGGA [AT]^CCGG[AT] BseAI 2071 TCCGGA T^CCGGA BsmBI 172 GAGACG CGTCTC(1/5) Bsp1407I 1397 TGTACA T^GTACA BspEI 2071 TCCGGA T^CCGGA BsrDI 767 GCAATG GCAATG(2/0) BsrFI 815 GCCGGC [GA]^CCGG[CT] BsrGI 1397 TGTACA T^GTACA BssHII 15 GCGCGC G^CGCGC BstEII 607 GGTGACC G^GT[ACGT]ACC BstPI 607 GGTGACC G^GT[ACGT]ACC BstXI 665 CCACAGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 815 GCCGGC [GA]^CCGG[CT] DraI 1056 TTTAAA TTT^AAA DrdI 2113 GACAACACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 155 TGGCCA [CT]^GGCC[GA] EcoNI 988 CCTGTGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 607 GGTGACC G^GT[ACGT]ACC EcoRV 1850 GATATC GAT^ATC FbaI 1314 TGATCA T^GATCA HincII 1084 GTCAAC GT[CT]^[GA]AC HindII 1084 GTCAAC GT[CT]^[GA]AC KspI 257 CCGCGG CCGC^GG MamI 1299 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1964 CAATTG C^AATTG MluNI 155 TGGCCA TGG^CCA MroI 2071 TCCGGA T^CCGGA MscI 155 TGGCCA TGG^CCA MunI 1964 CAATTG C^AATTG NaeI 815 GCCGGC GCC^GGC NgoMIV 815 GCCGGC G^CCGGC PflMI 635 CCAGAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Psp1406I 858 AACGTT AA^CGTT PvuII 49 CAGCTG CAG^CTG SacI 954 GAGCTC GAGCT^C SacII 257 CCGCGG CCGC^GG SapI 2144 GCTCTTC GCTCTTC(1/4) SmlI 732 CTTAAG C^T[CT][GA]AG SphI 95 GCATGC GCATG^C SspBI 1397 TGTACA T^GTACA Van91I 635 CCAGAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 1637 GAAATAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccI 1821 GTCTAC GT^[AC][GT]AC 1946 GTCTAC AccII 16 CGCG CG^CG 258 CGCG AcsI 191 GAATTT [GA]^AATT[CT] 1747 GAATTT AcyI 174 GACGCC G[GA]^CG[CT]C 2075 GACGCC AlwNI 49 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG 100 CAGCGGCTG ApoI 191 GAATTT [GA]^AATT[CT] 1747 GAATTT AspHI 825 GAGCAC G[AT]GC[AT]^C 954 GAGCTC BanI 1538 GGTGCC G^G[CT][GA]CC 2095 GGTGCC BbsI 40 GAAGAC GAAGAC(2/6) 203 GAAGAC BpuAI 40 GAAGAC GAAGAC(2/6) 203 GAAGAC BsaHI 174 GACGCC G[GA]^CG[CT]C 2075 GACGCC BsaI 712 GAGACC GGTCTC(1/5) 2132 GAGACC BsiHKAI 825 GAGCAC G[AT]GC[AT]^C 954 GAGCTC BspLU11I 1394 ACATGT A^CATGT 1984 ACATGT BstUI 16 CGCG CG^CG 258 CGCG DraII 297 GGGTCCT [GA]G^G[ACGT]CC[CT] 1407 GGGTCCT EcoO109I 297 GGGTCCT [GA]G^G[ACGT]CC[CT] 1407 GGGTCCT HgaI 174 GACGC GACGC(5/10) 2075 GACGC Hin1I 174 GACGCC G[GA]^CG[CT]C 2075 GACGCC HindIII 1070 AAGCTT A^AGCTT 1756 AAGCTT MvnI 16 CGCG CG^CG 258 CGCG NcoI 20 CCATGG C^CATGG 1275 CCATGG PpuMI 297 GGGTCCT [GA]G^G[AT]CC[CT] 1407 GGGTCCT SspI 1331 AATATT AAT^ATT 1650 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 84 enzymes) # ######################################### AciI AfaI AflIII AluI AlwI AvaII BanII BbvI BcnI BfaI BmyI BpmI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BssSI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DsaI EarI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII