(Circular) (Six-base) MAPSORT of: pFN21AE9254.seq Check: 9804 from: 1 to: 6949 pFN21AE9254 6949 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2416 2569 2416 Size: 153 6796 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4759 278 Size: 53 83 186 936 3223 2468 Fragments arranged by size: 3223 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3827 3952 4292 1937 Size: 1890 125 340 4594 Fragments arranged by size: 4594 1890 340 125 Acc65I G'GTAC_C Cuts at: 4270 4270 Size: 6949 AclI AA'CG_TT Cuts at: 1563 2864 5076 5449 1563 Size: 1301 2212 373 3063 Fragments arranged by size: 3063 2212 1301 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3419 5012 6396 6664 1632 Size: 1787 1593 1384 268 1917 Fragments arranged by size: 1917 1787 1593 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2737 820 Size: 197 1720 5032 Fragments arranged by size: 5032 1720 197 AflIII A'CryG_T Cuts at: 1203 2530 3399 3989 4185 5848 6825 1203 Size: 1327 869 590 196 1663 977 1327 Fragments arranged by size: 1663 1327 1327 977 869 590 196 AgeI A'CCGG_T Cuts at: 5780 5780 Size: 6949 AhdI GACnn_n'nnGTC Cuts at: 5678 5678 Size: 6949 AleI CACnn'nnGTG Cuts at: 1166 2147 1166 Size: 981 5968 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3559 1184 Size: 2375 4574 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4019 4051 4019 Size: 32 6917 AlwNI CAG_nnn'CTG Cuts at: 2059 2110 4692 6264 2059 Size: 51 2582 1572 2744 Fragments arranged by size: 2744 2582 1572 51 ApaLI G'TGCA_C Cuts at: 5006 6162 5006 Size: 1156 5793 ApoI r'AATT_y Cuts at: 1493 2196 3752 4258 4484 4636 6745 1493 Size: 703 1556 506 226 152 2109 1697 Fragments arranged by size: 2109 1697 1556 703 506 226 152 AseI AT'TA_AT Cuts at: 160 1032 5503 160 Size: 872 4471 1606 Fragments arranged by size: 4471 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6949 AvaI C'yCGr_G Cuts at: 1870 1943 4274 1870 Size: 73 2331 4545 Fragments arranged by size: 4545 2331 73 AvrII C'CTAG_G Cuts at: 3514 3514 Size: 6949 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2572 2605 2572 Size: 33 6916 BamHI G'GATC_C Cuts at: 2650 4279 4646 2650 Size: 1629 367 4953 Fragments arranged by size: 4953 1629 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3543 4100 4270 5725 618 Size: 325 217 627 1756 557 170 1455 1842 Fragments arranged by size: 1842 1756 1455 627 557 325 217 170 BanII G_rGCy'C Cuts at: 1391 2963 3962 4076 4268 1391 Size: 1572 999 114 192 4072 Fragments arranged by size: 4072 1572 999 192 114 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2052 2215 928 Size: 971 153 163 5662 Fragments arranged by size: 5662 971 163 153 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3164 3198 5140 5174 1903 Size: 34 50 34 1143 34 1942 34 3678 Fragments arranged by size: 3678 1942 1143 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2573 4841 6051 2573 Size: 2268 1210 3471 Fragments arranged by size: 3471 2268 1210 BclI T'GATC_A Cuts at: 1548 3319 1548 Size: 1771 5178 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3438 5560 136 Size: 107 122 71 631 605 1766 2122 1525 Fragments arranged by size: 2122 1766 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3524 6944 3524 Size: 3420 3529 BlpI GC'TnA_GC Cuts at: 4366 4366 Size: 6949 Bme1580I G_kGCm'C Cuts at: 1790 5010 6166 1790 Size: 3220 1156 2573 Fragments arranged by size: 3220 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2041 2370 4188 1318 Size: 723 329 1818 4079 Fragments arranged by size: 4079 1818 723 329 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3965 4680 5638 Size: 416 53 83 399 24 2548 715 958 Cuts at: 5638 442 Size: 1753 Fragments arranged by size: 2548 1753 958 715 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6949 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2332 2332 Size: 6949 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2581 2872 3163 5609 1239 Size: 1342 291 291 2446 2579 Fragments arranged by size: 2579 2446 1342 291 291 Bpu10I CC'TnA_GC Cuts at: 2069 2307 2401 2069 Size: 238 94 6617 Fragments arranged by size: 6617 238 94 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5074 5939 6237 5074 Size: 865 298 5786 Fragments arranged by size: 5786 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2711 4131 5612 882 Size: 617 1212 1420 1481 2219 Fragments arranged by size: 2219 1481 1420 1212 617 BsaAI yAC'GTr Cuts at: 493 1204 2531 4740 493 Size: 711 1327 2209 2702 Fragments arranged by size: 2702 2209 1327 711 BsaBI GATnn'nnATC Cuts at: 3308 6943 3308 Size: 3635 3314 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2180 4081 4756 5138 Size: 53 83 186 936 647 1901 675 382 Cuts at: 5138 5773 275 Size: 635 1451 Fragments arranged by size: 1901 1451 936 675 647 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4076 5382 5780 6054 6201 6612 4076 Size: 1306 398 274 147 411 4413 Fragments arranged by size: 4413 1306 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2690 2720 2870 2900 3161 Size: 30 165 30 1287 30 150 30 261 Cuts at: 3161 3191 1178 Size: 30 4936 Fragments arranged by size: 4936 1287 261 165 150 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2239 2908 2971 4191 1186 Size: 1053 669 63 1220 3944 Fragments arranged by size: 3944 1220 1053 669 63 BseYI C'CCAG_C Cuts at: 2008 2617 3677 6152 6648 2008 Size: 609 1060 2475 496 2309 Fragments arranged by size: 2475 2309 1060 609 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2505 2523 2792 3961 1163 Size: 573 769 18 269 1169 4151 Fragments arranged by size: 4151 1169 769 573 269 18 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4399 5160 6188 1540 Size: 275 186 2398 761 1028 2301 Fragments arranged by size: 2398 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2834 2963 5010 5095 6166 2834 Size: 129 2047 85 1071 3617 Fragments arranged by size: 3617 2047 1071 129 85 BsmI GAATG_Cn' Cuts at: 4460 4553 4460 Size: 93 6856 BsmBI CGTCTCn'nnnn_ Cuts at: 2171 2171 Size: 6949 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2834 2963 3962 4076 4268 5010 5095 Size: 399 1044 129 999 114 192 742 85 Cuts at: 5095 6166 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 1044 999 742 399 192 129 114 85 BspEI T'CCGG_A Cuts at: 4076 6612 4076 Size: 2536 4413 BspHI T'CATG_A Cuts at: 4838 6568 4838 Size: 1730 5219 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2416 2495 2569 3527 4304 844 Size: 1572 79 74 958 777 3489 Fragments arranged by size: 3489 1572 958 777 79 74 BsrBI CCG'CTC Cuts at: 1394 1823 4836 1394 Size: 429 3013 3507 Fragments arranged by size: 3507 3013 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2779 5444 5618 65 Size: 1183 1531 2665 174 1396 Fragments arranged by size: 2665 1531 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2820 5593 5780 1700 Size: 110 1010 2773 187 2869 Fragments arranged by size: 2869 2773 1010 187 110 BsrGI T'GTAC_A Cuts at: 96 3402 96 Size: 3306 3643 BssHII G'CGCG_C Cuts at: 1925 2020 1925 Size: 95 6854 BssSI C'ACGA_G Cuts at: 3999 4009 4021 5009 6021 3999 Size: 10 12 988 1012 4927 Fragments arranged by size: 4927 1012 988 12 10 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6949 BstBI TT'CG_AA Cuts at: 5753 5753 Size: 6949 BstEII G'GTnAC_C Cuts at: 1184 2612 1184 Size: 1428 5521 BstXI CCAn_nnnn'nTGG Cuts at: 2677 2677 Size: 6949 BstYI r'GATC_y Cuts at: 1972 2238 2650 3115 3524 4279 4646 5031 5048 Size: 266 412 465 409 755 367 385 17 Cuts at: 5048 6500 6586 6598 6944 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 755 465 412 409 385 367 346 266 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2025 2262 3280 3718 513 Size: 552 960 237 1018 438 3744 Fragments arranged by size: 3744 1018 960 552 438 237 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6210 Fragments arranged by size: 6210 683 56 BtsI GCAGTG_nn' Cuts at: 2418 4465 5259 5279 2418 Size: 2047 794 20 4088 Fragments arranged by size: 4088 2047 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6949 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2172 2207 529 Size: 35 1608 35 5271 Fragments arranged by size: 5271 1608 35 35 DraI TTT'AAA Cuts at: 3063 4253 3063 Size: 1190 5759 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4124 5956 809 Size: 507 2808 1832 1802 Fragments arranged by size: 2808 1832 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2160 4396 5285 Size: 54 1425 50 275 18 330 2236 889 Cuts at: 5285 6914 8 Size: 1629 43 Fragments arranged by size: 2236 1629 1425 889 330 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4396 1537 Size: 275 2584 4090 Fragments arranged by size: 4090 2584 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2376 4156 4164 4197 4879 1353 Size: 1023 1780 8 33 682 3423 Fragments arranged by size: 3423 1780 1023 682 33 8 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5536 5910 6056 387 Size: 1064 138 3947 374 146 1280 Fragments arranged by size: 3947 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2961 2961 Size: 6949 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2581 2872 3163 3419 5012 5609 6396 Size: 393 949 291 291 256 1593 597 787 Cuts at: 6396 6664 1239 Size: 268 1524 Fragments arranged by size: 1593 1524 949 787 597 393 291 291 268 256 EcoNI CCTnn'n_nnAGG Cuts at: 2997 2997 Size: 6949 EcoO109I rG'GnC_Cy Cuts at: 2303 3413 2303 Size: 1110 5839 EcoRI G'AATT_C Cuts at: 4258 4636 4258 Size: 378 6571 EcoRV GAT'ATC Cuts at: 3857 3857 Size: 6949 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2989 3098 3519 2989 Size: 109 421 6419 Fragments arranged by size: 6419 421 109 FspI TGC'GCA Cuts at: 5455 5455 Size: 6949 HaeII r_GCGC'y Cuts at: 1678 6096 1678 Size: 4418 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2354 2386 2690 2722 3087 Size: 32 135 32 782 32 304 32 365 Cuts at: 3087 3119 4015 4047 4172 4204 5590 5622 5664 Size: 32 896 32 125 32 1386 32 42 Cuts at: 5664 5696 1373 Size: 32 2626 Fragments arranged by size: 2626 1386 896 782 365 304 135 125 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3091 4293 4539 669 Size: 988 281 1153 1202 246 3079 Fragments arranged by size: 3079 1202 1153 988 281 246 HindIII A'AGCT_T Cuts at: 748 3075 3761 748 Size: 2327 686 3936 Fragments arranged by size: 3936 2327 686 HpaI GTT'AAC Cuts at: 4539 4539 Size: 6949 KpnI G_GTAC'C Cuts at: 4274 4274 Size: 6949 MfeI C'AATT_G Cuts at: 3969 4548 3969 Size: 579 6370 MluI A'CGCG_T Cuts at: 6825 6825 Size: 6949 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3104 6063 6247 1305 Size: 1799 2959 184 2007 Fragments arranged by size: 2959 2007 1799 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2162 10 Size: 54 1425 343 330 4797 Fragments arranged by size: 4797 1425 343 330 54 MslI CAynn'nnrTG Cuts at: 518 1166 2147 2675 3226 3398 3988 4907 5266 Size: 648 981 528 551 172 590 919 359 Cuts at: 5266 5425 518 Size: 159 2042 Fragments arranged by size: 2042 981 919 648 590 551 528 359 172 159 MspA1I CmG'CkG Cuts at: 1606 2056 2107 2264 2542 4365 5042 6190 6435 Size: 450 51 157 278 1823 677 1148 245 Cuts at: 6435 1606 Size: 2120 Fragments arranged by size: 2120 1823 1148 677 450 278 245 157 51 NaeI GCC'GGC Cuts at: 2822 2822 Size: 6949 NcoI C'CATG_G Cuts at: 513 1065 2025 3280 513 Size: 552 960 1255 4182 Fragments arranged by size: 4182 1255 960 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6949 NgoMIV G'CCGG_C Cuts at: 2820 2820 Size: 6949 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6949 NotI GC'GGCC_GC Cuts at: 4396 4396 Size: 6949 NspI r_CATG'y Cuts at: 1132 1229 2104 3403 3993 4307 5852 1132 Size: 97 875 1299 590 314 1545 2229 Fragments arranged by size: 2229 1545 1299 875 590 314 97 PasI CC'CwG_GG Cuts at: 1348 3934 4069 4096 1348 Size: 2586 135 27 4201 Fragments arranged by size: 4201 2586 135 27 PciI A'CATG_T Cuts at: 3399 3989 5848 3399 Size: 590 1859 4500 Fragments arranged by size: 4500 1859 590 PflMI CCAn_nnn'nTGG Cuts at: 1108 2646 1108 Size: 1538 5411 PfoI T'CCnGG_A Cuts at: 2241 2888 3378 2241 Size: 647 490 5812 Fragments arranged by size: 5812 647 490 PmeI GTTT'AAAC Cuts at: 4253 4253 Size: 6949 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5018 5050 6531 6563 5018 Size: 32 1481 32 5404 Fragments arranged by size: 5404 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2303 3413 2303 Size: 1110 5839 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6949 PsiI TTA'TAA Cuts at: 4519 4519 Size: 6949 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4175 4207 4175 Size: 32 6917 PstI C_TGCA'G Cuts at: 830 2064 2296 2413 2588 4301 830 Size: 1234 232 117 175 1713 3478 Fragments arranged by size: 3478 1713 1234 232 175 117 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6949 PvuII CAG'CTG Cuts at: 2056 2056 Size: 6949 SacI G_AGCT'C Cuts at: 2963 2963 Size: 6949 SacII CC_GC'GG Cuts at: 2265 2265 Size: 6949 SalI G'TCGA_C Cuts at: 1936 4291 1936 Size: 2355 4594 SapI GCTCTTCn'nnn_ Cuts at: 4156 4156 Size: 6949 SbfI CC_TGCA'GG Cuts at: 4301 4301 Size: 6949 ScaI AGT'ACT Cuts at: 5197 6909 5197 Size: 1712 5237 SfcI C'TryA_G Cuts at: 826 1045 2060 2292 2409 2584 3828 4297 5432 Size: 219 1015 232 117 175 1244 469 1135 Cuts at: 5432 6113 6304 826 Size: 681 191 1471 Fragments arranged by size: 1471 1244 1135 1015 681 469 232 219 191 175 117 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6949 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6949 SmaI CCC'GGG Cuts at: 1872 4276 1872 Size: 2404 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2737 5053 5954 6216 820 Size: 197 926 794 2316 901 262 1553 Fragments arranged by size: 2316 1553 926 901 794 262 197 SnaBI TAC'GTA Cuts at: 493 4740 493 Size: 4247 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6949 SphI G_CATG'C Cuts at: 1132 2104 4307 1132 Size: 972 2203 3774 Fragments arranged by size: 3774 2203 972 SspI AAT'ATT Cuts at: 5 52 3338 3657 4873 5 Size: 47 3286 319 1216 2081 Fragments arranged by size: 3286 2081 1216 319 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6949 StyI C'CwwG_G Cuts at: 513 1065 2025 2964 3280 3514 4388 513 Size: 552 960 939 316 234 874 3074 Fragments arranged by size: 3074 960 939 874 552 316 234 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3691 4088 4976 4993 5146 5331 5776 Size: 1255 2111 397 888 17 153 185 445 Cuts at: 5776 325 Size: 1498 Fragments arranged by size: 2111 1498 1255 888 445 397 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2121 3402 5195 Size: 274 80 33 51 1443 144 1281 1793 Cuts at: 5195 6907 96 Size: 1712 138 Fragments arranged by size: 1793 1712 1443 1281 274 144 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6909 6941 6909 Size: 32 6917 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6949 XbaI T'CTAG_A Cuts at: 4285 4285 Size: 6949 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6949 XmaI C'CCGG_G Cuts at: 1870 4274 1870 Size: 2404 4545 XmnI GAAnn'nnTTC Cuts at: 3646 5078 3646 Size: 1432 5517 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4757 276 Size: 53 83 186 936 3223 2468 Fragments arranged by size: 3223 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI BbeI BbvCI BfrBI BsiWI BstZ17I Bsu36I DraIII FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI RsrII SanDI SexAI SfiI SfoI SrfI SwaI XcmI