(Linear) (Six-base) MAPSORT of: KIEE9254.seq Check: 9518 from: 1 to: 2244 KIEE9254.orf With 182 enzymes: SgfI * December 27, 2011 16:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 411 564 2244 Size: 411 153 1680 Fragments arranged by size: 1680 411 153 AccI GT'mk_AC Cuts at: 0 1822 1947 2244 Size: 1822 125 297 Fragments arranged by size: 1822 297 125 AclI AA'CG_TT Cuts at: 0 859 2244 Size: 859 1385 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1414 2244 Size: 1414 830 AflII C'TTAA_G Cuts at: 0 732 2244 Size: 732 1512 AflIII A'CryG_T Cuts at: 0 525 1394 1984 2180 2244 Size: 525 869 590 196 64 Fragments arranged by size: 869 590 525 196 64 AleI CACnn'nnGTG Cuts at: 0 142 2244 Size: 142 2102 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1554 2244 Size: 1554 690 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2014 2046 2244 Size: 2014 32 198 Fragments arranged by size: 2014 198 32 AlwNI CAG_nnn'CTG Cuts at: 0 54 105 2244 Size: 54 51 2139 Fragments arranged by size: 2139 54 51 ApoI r'AATT_y Cuts at: 0 191 1747 2244 Size: 191 1556 497 Fragments arranged by size: 1556 497 191 AvrII C'CTAG_G Cuts at: 0 1509 2244 Size: 1509 735 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 567 600 2244 Size: 567 33 1644 Fragments arranged by size: 1644 567 33 BamHI G'GATC_C Cuts at: 0 645 2244 Size: 645 1599 BanI G'GyrC_C Cuts at: 0 1538 2095 2244 Size: 1538 557 149 Fragments arranged by size: 1538 557 149 BanII G_rGCy'C Cuts at: 0 958 1957 2071 2244 Size: 958 999 114 173 Fragments arranged by size: 999 958 173 114 BbsI GAAGACnn'nnnn_ Cuts at: 0 47 210 2244 Size: 47 163 2034 Fragments arranged by size: 2034 163 47 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1159 1193 2244 Size: 1159 34 1051 Fragments arranged by size: 1159 1051 34 BciVI GTATCCnnnnn_n' Cuts at: 0 568 2244 Size: 568 1676 BclI T'GATC_A Cuts at: 0 1314 2244 Size: 1314 930 BglI GCCn_nnn'nGGC Cuts at: 0 1433 2244 Size: 1433 811 BglII A'GATC_T Cuts at: 0 1519 2244 Size: 1519 725 BmgBI CAC'GTC Cuts at: 0 36 365 2183 2244 Size: 36 329 1818 61 Fragments arranged by size: 1818 329 61 36 BmrI ACTGGGnnnn_n' Cuts at: 0 1960 2244 Size: 1960 284 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 327 2244 Size: 327 1917 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 576 867 1158 2244 Size: 576 291 291 1086 Fragments arranged by size: 1086 576 291 291 Bpu10I CC'TnA_GC Cuts at: 0 64 302 396 2244 Size: 64 238 94 1848 Fragments arranged by size: 1848 238 94 64 BsaI GGTCTCn'nnnn_ Cuts at: 0 706 2126 2244 Size: 706 1420 118 Fragments arranged by size: 1420 706 118 BsaAI yAC'GTr Cuts at: 0 526 2244 Size: 526 1718 BsaBI GATnn'nnATC Cuts at: 0 1303 2244 Size: 1303 941 BsaHI Gr'CG_yC Cuts at: 0 175 2076 2244 Size: 175 1901 168 Fragments arranged by size: 1901 175 168 BsaWI w'CCGG_w Cuts at: 0 2071 2244 Size: 2071 173 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 685 715 865 895 1156 1186 2244 Size: 685 30 150 30 261 30 1058 Fragments arranged by size: 1058 685 261 150 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 234 903 966 2186 2244 Size: 234 669 63 1220 58 Fragments arranged by size: 1220 669 234 63 58 BseYI C'CCAG_C Cuts at: 0 3 612 1672 2244 Size: 3 609 1060 572 Fragments arranged by size: 1060 609 572 3 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 500 518 787 1956 2244 Size: 500 18 269 1169 288 Fragments arranged by size: 1169 500 288 269 18 BsiHKAI G_wGCw'C Cuts at: 0 829 958 2244 Size: 829 129 1286 Fragments arranged by size: 1286 829 129 BsmBI CGTCTCn'nnnn_ Cuts at: 0 166 2244 Size: 166 2078 Bsp1286I G_dGCh'C Cuts at: 0 829 958 1957 2071 2244 Size: 829 129 999 114 173 Fragments arranged by size: 999 829 173 129 114 BspEI T'CCGG_A Cuts at: 0 2071 2244 Size: 2071 173 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 411 490 564 1522 2244 Size: 411 79 74 958 722 Fragments arranged by size: 958 722 411 79 74 BsrDI GCAATG_nn' Cuts at: 0 774 2244 Size: 774 1470 BsrFI r'CCGG_y Cuts at: 0 815 2244 Size: 815 1429 BsrGI T'GTAC_A Cuts at: 0 1397 2244 Size: 1397 847 BssHII G'CGCG_C Cuts at: 0 15 2244 Size: 15 2229 BssSI C'ACGA_G Cuts at: 0 1994 2004 2016 2244 Size: 1994 10 12 228 Fragments arranged by size: 1994 228 12 10 BstEII G'GTnAC_C Cuts at: 0 607 2244 Size: 607 1637 BstXI CCAn_nnnn'nTGG Cuts at: 0 672 2244 Size: 672 1572 BstYI r'GATC_y Cuts at: 0 233 645 1110 1519 2244 Size: 233 412 465 409 725 Fragments arranged by size: 725 465 412 409 233 BtgI C'CryG_G Cuts at: 0 20 257 1275 1713 2244 Size: 20 237 1018 438 531 Fragments arranged by size: 1018 531 438 237 20 BtsI GCAGTG_nn' Cuts at: 0 413 2244 Size: 413 1831 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 167 202 2244 Size: 167 35 2042 Fragments arranged by size: 2042 167 35 DraI TTT'AAA Cuts at: 0 1058 2244 Size: 1058 1186 DrdI GACnn_nn'nnGTC Cuts at: 0 2119 2244 Size: 2119 125 EaeI y'GGCC_r Cuts at: 0 155 2244 Size: 155 2089 EarI CTCTTCn'nnn_ Cuts at: 0 371 2151 2159 2192 2244 Size: 371 1780 8 33 52 Fragments arranged by size: 1780 371 52 33 8 EcoICRI GAG'CTC Cuts at: 0 956 2244 Size: 956 1288 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 576 867 1158 1414 2244 Size: 576 291 291 256 830 Fragments arranged by size: 830 576 291 291 256 EcoNI CCTnn'n_nnAGG Cuts at: 0 992 2244 Size: 992 1252 EcoO109I rG'GnC_Cy Cuts at: 0 298 1408 2244 Size: 298 1110 836 Fragments arranged by size: 1110 836 298 EcoRV GAT'ATC Cuts at: 0 1852 2244 Size: 1852 392 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 984 1093 1514 2244 Size: 984 109 421 730 Fragments arranged by size: 984 730 421 109 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 349 381 685 717 1082 1114 2010 2042 Size: 349 32 304 32 365 32 896 32 Cuts at: 2042 2167 2199 2244 Size: 125 32 45 Fragments arranged by size: 896 365 349 304 125 45 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 1086 2244 Size: 1086 1158 HindIII A'AGCT_T Cuts at: 0 1070 1756 2244 Size: 1070 686 488 Fragments arranged by size: 1070 686 488 MfeI C'AATT_G Cuts at: 0 1964 2244 Size: 1964 280 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1099 2244 Size: 1099 1145 MscI TGG'CCA Cuts at: 0 157 2244 Size: 157 2087 MslI CAynn'nnrTG Cuts at: 0 142 670 1221 1393 1983 2244 Size: 142 528 551 172 590 261 Fragments arranged by size: 590 551 528 261 172 142 MspA1I CmG'CkG Cuts at: 0 51 102 259 537 2244 Size: 51 51 157 278 1707 Fragments arranged by size: 1707 278 157 51 51 NaeI GCC'GGC Cuts at: 0 817 2244 Size: 817 1427 NcoI C'CATG_G Cuts at: 0 20 1275 2244 Size: 20 1255 969 Fragments arranged by size: 1255 969 20 NgoMIV G'CCGG_C Cuts at: 0 815 2244 Size: 815 1429 NspI r_CATG'y Cuts at: 0 99 1398 1988 2244 Size: 99 1299 590 256 Fragments arranged by size: 1299 590 256 99 PasI CC'CwG_GG Cuts at: 0 1929 2064 2091 2244 Size: 1929 135 27 153 Fragments arranged by size: 1929 153 135 27 PciI A'CATG_T Cuts at: 0 1394 1984 2244 Size: 1394 590 260 Fragments arranged by size: 1394 590 260 PflMI CCAn_nnn'nTGG Cuts at: 0 641 2244 Size: 641 1603 PfoI T'CCnGG_A Cuts at: 0 236 883 1373 2244 Size: 236 647 490 871 Fragments arranged by size: 871 647 490 236 PpuMI rG'GwC_Cy Cuts at: 0 298 1408 2244 Size: 298 1110 836 Fragments arranged by size: 1110 836 298 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 2170 2202 2244 Size: 2170 32 42 Fragments arranged by size: 2170 42 32 PstI C_TGCA'G Cuts at: 0 59 291 408 583 2244 Size: 59 232 117 175 1661 Fragments arranged by size: 1661 232 175 117 59 PvuII CAG'CTG Cuts at: 0 51 2244 Size: 51 2193 SacI G_AGCT'C Cuts at: 0 958 2244 Size: 958 1286 SacII CC_GC'GG Cuts at: 0 260 2244 Size: 260 1984 SapI GCTCTTCn'nnn_ Cuts at: 0 2151 2244 Size: 2151 93 SfcI C'TryA_G Cuts at: 0 55 287 404 579 1823 2244 Size: 55 232 117 175 1244 421 Fragments arranged by size: 1244 421 232 175 117 55 SmlI C'TyrA_G Cuts at: 0 732 2244 Size: 732 1512 SphI G_CATG'C Cuts at: 0 99 2244 Size: 99 2145 SspI AAT'ATT Cuts at: 0 1333 1652 2244 Size: 1333 319 592 Fragments arranged by size: 1333 592 319 StyI C'CwwG_G Cuts at: 0 20 959 1275 1509 2244 Size: 20 939 316 234 735 Fragments arranged by size: 939 735 316 234 20 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1686 2083 2244 Size: 1686 397 161 Fragments arranged by size: 1686 397 161 TatI w'GTAC_w Cuts at: 0 116 1397 2244 Size: 116 1281 847 Fragments arranged by size: 1281 847 116 XmnI GAAnn'nnTTC Cuts at: 0 1641 2244 Size: 1641 603 Enzymes that do cut: AarI AccI AclI AcuI AflII AflIII AleI AlfI AloI AlwNI ApoI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BmgBI BmrI BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrDI BsrFI BsrGI BssHII BssSI BstEII BstXI BstYI BtgI BtsI CspCI DraI DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI Hin4I HincII HindIII MfeI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI PasI PciI PflMI PfoI PpuMI PsrI PstI PvuII SacI SacII SapI SfcI SmlI SphI SspI StyI TaqII TatI XmnI Enzymes that do not cut: AatII Acc65I AfeI AgeI AhdI ApaI ApaLI AscI AseI AsiSI AvaI BbeI BbvCI BfrBI BlpI Bme1580I BmtI BpuEI BsiEI BsiWI BsmI BspHI BsrBI BstAPI BstBI BstZ17I Bsu36I BtgZI ClaI DraIII EagI EciI EcoRI FseI FspI FspAI HaeII HpaI KasI KpnI MluI NarI NdeI NheI NotI NruI NsiI PacI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PvuI RsrII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI StuI SwaI TstI Tth111I XbaI XcmI XhoI XmaI ZraI