################################## # NO CUT ENZYMES ( 156 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanI BbeI BbsI BbvCI BcgI BclI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MslI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmeI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AflIII 385 ACACGT A^C[GA][CT]GT ApaI 431 GGGCCC GGGCC^C Asp700I 139 GAATCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 872 CCCGGG C^[CT]CG[GA]G AvaII 648 GGTCC G^G[AT]CC BanII 431 GGGCCC G[GA]GC[CT]^C BbrPI 734 CACGTG CAC^GTG BciVI 878 GTATCC GTATCC(6/5) BfaI 101 CTAG C^TAG BsaAI 734 CACGTG [CT]AC^GT[GA] BsmAI 97 GAGAC GTCTC(1/5) BsmFI 389 GTCCC GGGAC(10/14) BsmI 171 GCATTC GAATGC(1/-1) BsoBI 872 CCCGGG C^[CT]CG[GA]G BspMI 845 ACCTGC ACCTGC(4/8) BstEII 861 GGTCACC G^GT[ACGT]ACC BstPI 861 GGTCACC G^GT[ACGT]ACC BstXI 629 CCATGATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 22 GCGC GCG^C EcoNI 846 CCTGCAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 861 GGTCACC G^GT[ACGT]ACC EcoT14I 417 CCTTGG C^C[AT][AT]GG HhaI 22 GCGC GCG^C HinP1I 22 GCGC G^CGC MaeI 101 CTAG C^TAG MseI 475 TTAA T^TAA MspA1I 766 CAGCTG C[AC]G^C[GT]G NspI 690 GCATGT [GA]CATG^[CT] PflMI 161 CCATGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 734 CACGTG CAC^GTG PmlI 734 CACGTG CAC^GTG PstI 652 CTGCAG CTGCA^G PvuII 766 CAGCTG CAG^CTG SexAI 316 ACCAGGT A^CC[AT]GGT SfaNI 301 GCATC GCATC(5/9) SfcI 652 CTGCAG C^T[GA][CT]AG SmaI 872 CCCGGG CCC^GGG SspI 214 AATATT AAT^ATT StyI 417 CCTTGG C^C[AT][AT]GG TaqI 711 TCGA T^CGA Tru9I 475 TTAA T^TAA Tsp45I 862 GTCAC ^GT[GC]AC TthHB8I 711 TCGA T^CGA Van91I 161 CCATGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 872 CCCGGG C^CCGGG XmaI 872 CCCGGG C^CCGGG XmnI 139 GAATCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AfaI 182 GTAC GT^AC 357 GTAC AlwI 868 GATCC GGATC(4/5) 74 GGATC BcnI 872 CCCGG CC^[GC]GG 873 CCGGG BglII 62 AGATCT A^GATCT 563 AGATCT BmyI 431 GGGCCC G[AGT]GC[ACT]^C 731 GGGCAC BpmI 556 CTCCAG CTGGAG(16/14) 118 CTGGAG Bsp1286I 431 GGGCCC G[AGT]GC[ACT]^C 731 GGGCAC BsrDI 272 GCAATG GCAATG(2/0) 291 GCAATG BstYI 62 AGATCT [GA]^GATC[CT] 563 AGATCT Cac8I 821 GCTGGC GC[ACGT]^[ACGT]GC 825 GCTTGC DraII 430 GGGGCCC [GA]G^G[ACGT]CC[CT] 431 GGGCCCT EcoO109I 430 GGGGCCC [GA]G^G[ACGT]CC[CT] 431 GGGCCCT HaeIII 432 GGCC GG^CC 856 GGCC HapII 405 CCGG C^CGG 873 CCGG HinfI 139 GAATC G^A[ACGT]TC 514 GAATC HpaII 405 CCGG C^CGG 873 CCGG MaeIII 704 GTTAC ^GT[ACGT]AC 862 GTCAC MboII 561 GAAGA GAAGA(8/7) 840 GAAGA MflI 62 AGATCT [GA]^GATC[CT] 563 AGATCT MspI 405 CCGG C^CGG 873 CCGG NciI 872 CCCGG CC^[GC]GG 873 CCGGG NlaIV 430 GGGGCC GG[ACGT]^[ACGT]CC 431 GGGCCC RsaI 182 GTAC GT^AC 357 GTAC TfiI 139 GAATC G^A[AT]TC 514 GAATC XhoII 62 AGATCT [GA]^GATC[CT] 563 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 30 enzymes) # ######################################### AluI BbvI BsaJI BsiYI BslI BsrI BssKI BstF5I BstNI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HphI ItaI MaeII MboI MnlI MvaI MwoI NdeII NlaIII Sau3AI Sau96I ScrFI TseI Tsp509I TspRI