(Circular) (Six-base) MAPSORT of: pFN21AE3120.seq Check: 1197 from: 1 to: 5602 pFN21AE3120 5602 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3412 278 Size: 53 83 186 936 1876 2468 Fragments arranged by size: 2468 1876 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2945 1937 Size: 1008 4594 Acc65I G'GTAC_C Cuts at: 2923 2923 Size: 5602 AclI AA'CG_TT Cuts at: 1563 3729 4102 1563 Size: 2166 373 3063 Fragments arranged by size: 3063 2166 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2711 3665 5049 5317 1632 Size: 1079 954 1384 268 1917 Fragments arranged by size: 1917 1384 1079 954 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5405 AflIII A'CryG_T Cuts at: 1203 2390 4501 5478 1203 Size: 1187 2111 977 1327 Fragments arranged by size: 2111 1327 1187 977 AgeI A'CCGG_T Cuts at: 4433 4433 Size: 5602 AhdI GACnn_n'nnGTC Cuts at: 4331 4331 Size: 5602 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5602 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5602 AlwNI CAG_nnn'CTG Cuts at: 3345 4917 3345 Size: 1572 4030 ApaI G_GGCC'C Cuts at: 2440 2440 Size: 5602 ApaLI G'TGCA_C Cuts at: 3659 4815 3659 Size: 1156 4446 ApoI r'AATT_y Cuts at: 1493 2911 3137 3289 5398 1493 Size: 1418 226 152 2109 1697 Fragments arranged by size: 2109 1697 1418 226 152 AseI AT'TA_AT Cuts at: 160 1032 4156 160 Size: 872 3124 1606 Fragments arranged by size: 3124 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5602 AvaI C'yCGr_G Cuts at: 1870 1943 2877 2927 1870 Size: 73 934 50 4545 Fragments arranged by size: 4545 934 73 50 BamHI G'GATC_C Cuts at: 2932 3299 2932 Size: 367 5235 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2923 4378 618 Size: 325 217 627 1136 1455 1842 Fragments arranged by size: 1842 1455 1136 627 325 217 BanII G_rGCy'C Cuts at: 1391 2440 2921 1391 Size: 1049 481 4072 Fragments arranged by size: 4072 1049 481 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4631 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3793 3827 1903 Size: 34 50 34 1772 34 3678 Fragments arranged by size: 3678 1772 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2894 3494 4704 2894 Size: 600 1210 3792 Fragments arranged by size: 3792 1210 600 BclI T'GATC_A Cuts at: 1548 1548 Size: 5602 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4213 136 Size: 107 122 71 631 605 2541 1525 Fragments arranged by size: 2541 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2067 2568 5597 2067 Size: 501 3029 2072 Fragments arranged by size: 3029 2072 501 BlpI GC'TnA_GC Cuts at: 3019 3019 Size: 5602 Bme1580I G_kGCm'C Cuts at: 1790 2440 2740 3663 4819 1790 Size: 650 300 923 1156 2573 Fragments arranged by size: 2573 1156 923 650 300 BmgBI CAC'GTC Cuts at: 1318 2393 1318 Size: 1075 4527 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3333 4291 442 Size: 416 53 83 399 24 1916 958 1753 Fragments arranged by size: 1916 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5602 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2638 2638 Size: 5602 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2144 2546 4262 1239 Size: 905 402 1716 2579 Fragments arranged by size: 2579 1716 905 402 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3727 4592 4890 3727 Size: 865 298 4439 Fragments arranged by size: 4439 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4265 882 Size: 617 2766 2219 Fragments arranged by size: 2766 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2741 3393 493 Size: 711 1537 652 2702 Fragments arranged by size: 2702 1537 711 652 BsaBI GATnn'nnATC Cuts at: 5596 5596 Size: 5602 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3409 3791 4426 275 Size: 53 83 186 936 1876 382 635 1451 Fragments arranged by size: 1876 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4035 4433 4707 4854 5265 4035 Size: 398 274 147 411 4372 Fragments arranged by size: 4372 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2745 2775 1178 Size: 30 165 30 1342 30 4005 Fragments arranged by size: 4005 1342 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5602 BseYI C'CCAG_C Cuts at: 4805 5301 4805 Size: 496 5106 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5029 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3052 3813 4841 1540 Size: 275 186 1051 761 1028 2301 Fragments arranged by size: 2301 1051 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3663 3748 4819 3663 Size: 85 1071 4446 Fragments arranged by size: 4446 1071 85 BsmI GAATG_Cn' Cuts at: 2176 3113 3206 2176 Size: 937 93 4572 Fragments arranged by size: 4572 937 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2440 2740 2921 3663 3748 4819 1391 Size: 399 650 300 181 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 650 399 300 181 85 BspEI T'CCGG_A Cuts at: 5265 5265 Size: 5602 BspHI T'CATG_A Cuts at: 3491 5221 3491 Size: 1730 3872 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2859 2957 844 Size: 2015 98 3489 Fragments arranged by size: 3489 2015 98 BsrBI CCG'CTC Cuts at: 1394 1823 3489 1394 Size: 429 1666 3507 Fragments arranged by size: 3507 1666 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2284 2303 4097 4271 65 Size: 1183 1036 19 1794 174 1396 Fragments arranged by size: 1794 1396 1183 1036 174 19 BsrFI r'CCGG_y Cuts at: 1700 1810 4246 4433 1700 Size: 110 2436 187 2869 Fragments arranged by size: 2869 2436 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5602 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5602 BssSI C'ACGA_G Cuts at: 3662 4674 3662 Size: 1012 4590 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5602 BstBI TT'CG_AA Cuts at: 4406 4406 Size: 5602 BstEII G'GTnAC_C Cuts at: 1184 2866 1184 Size: 1682 3920 BstXI CCAn_nnnn'nTGG Cuts at: 2641 2641 Size: 5602 BstYI r'GATC_y Cuts at: 1972 2067 2568 2932 3299 3684 3701 5153 5239 Size: 95 501 364 367 385 17 1452 86 Cuts at: 5239 5251 5597 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 501 385 367 364 346 95 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2898 2898 Size: 5602 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5050 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4863 Fragments arranged by size: 4863 683 56 BtsI GCAGTG_nn' Cuts at: 3118 3912 3932 3118 Size: 794 20 4788 Fragments arranged by size: 4788 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5602 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2229 2264 2340 2375 529 Size: 35 1665 35 76 35 3756 Fragments arranged by size: 3756 1665 76 35 35 35 DraI TTT'AAA Cuts at: 2906 2906 Size: 5602 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4609 809 Size: 507 3293 1802 Fragments arranged by size: 3293 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3049 3938 5567 Size: 54 1425 50 275 18 1219 889 1629 Cuts at: 5567 8 Size: 43 Fragments arranged by size: 1629 1425 1219 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3049 1537 Size: 275 1237 4090 Fragments arranged by size: 4090 1237 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3532 1353 Size: 2179 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4189 4563 4709 387 Size: 1064 138 2600 374 146 1280 Fragments arranged by size: 2600 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2144 2546 2711 3665 4262 5049 5317 Size: 393 512 402 165 954 597 787 268 Cuts at: 5317 1239 Size: 1524 Fragments arranged by size: 1524 954 787 597 512 402 393 268 165 EcoNI CCTnn'n_nnAGG Cuts at: 2855 2855 Size: 5602 EcoO109I rG'GnC_Cy Cuts at: 2436 2437 2436 Size: 1 5601 EcoRI G'AATT_C Cuts at: 2911 3289 2911 Size: 378 5224 FspI TGC'GCA Cuts at: 4108 4108 Size: 5602 HaeII r_GCGC'y Cuts at: 1678 4749 1678 Size: 3071 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2052 2084 2745 2777 4243 Size: 32 135 32 480 32 661 32 1466 Cuts at: 4243 4275 4317 4349 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1466 661 480 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2946 3192 669 Size: 988 281 1008 246 3079 Fragments arranged by size: 3079 1008 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5602 HpaI GTT'AAC Cuts at: 3192 3192 Size: 5602 KpnI G_GTAC'C Cuts at: 2927 2927 Size: 5602 MfeI C'AATT_G Cuts at: 3201 3201 Size: 5602 MluI A'CGCG_T Cuts at: 5478 5478 Size: 5602 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4716 4900 1305 Size: 3411 184 2007 Fragments arranged by size: 3411 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3780 Fragments arranged by size: 3780 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3560 3919 4078 518 Size: 648 2394 359 159 2042 Fragments arranged by size: 2394 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2773 3018 3695 4843 5088 1606 Size: 1167 245 677 1148 245 2120 Fragments arranged by size: 2120 1167 1148 677 245 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5050 NdeI CA'TA_TG Cuts at: 387 387 Size: 5602 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5602 NotI GC'GGCC_GC Cuts at: 3049 3049 Size: 5602 NspI r_CATG'y Cuts at: 1132 1229 2699 2960 4505 1132 Size: 97 1470 261 1545 2229 Fragments arranged by size: 2229 1545 1470 261 97 PasI CC'CwG_GG Cuts at: 1348 2733 1348 Size: 1385 4217 PciI A'CATG_T Cuts at: 4501 4501 Size: 5602 PflMI CCAn_nnn'nTGG Cuts at: 1108 2172 1108 Size: 1064 4538 PmeI GTTT'AAAC Cuts at: 2906 2906 Size: 5602 PmlI CAC'GTG Cuts at: 2741 2741 Size: 5602 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2133 2165 3671 3703 5184 5216 2133 Size: 32 1506 32 1481 32 2519 Fragments arranged by size: 2519 1506 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5602 PsiI TTA'TAA Cuts at: 3172 3172 Size: 5602 PspOMI G'GGCC_C Cuts at: 2436 2436 Size: 5602 PstI C_TGCA'G Cuts at: 830 2661 2954 830 Size: 1831 293 3478 Fragments arranged by size: 3478 1831 293 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5602 PvuII CAG'CTG Cuts at: 2773 2773 Size: 5602 SalI G'TCGA_C Cuts at: 1936 2944 1936 Size: 1008 4594 SbfI CC_TGCA'GG Cuts at: 2954 2954 Size: 5602 ScaI AGT'ACT Cuts at: 3850 5562 3850 Size: 1712 3890 SexAI A'CCwGG_T Cuts at: 2321 2321 Size: 5602 SfcI C'TryA_G Cuts at: 826 1045 2657 2950 4085 4766 4957 826 Size: 219 1612 293 1135 681 191 1471 Fragments arranged by size: 1612 1471 1135 681 293 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5602 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5602 SmaI CCC'GGG Cuts at: 1872 2879 2929 1872 Size: 1007 50 4545 Fragments arranged by size: 4545 1007 50 SmlI C'TyrA_G Cuts at: 820 1017 1943 3706 4607 4869 820 Size: 197 926 1763 901 262 1553 Fragments arranged by size: 1763 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3393 493 Size: 2900 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5602 SphI G_CATG'C Cuts at: 1132 2960 1132 Size: 1828 3774 SspI AAT'ATT Cuts at: 5 52 2221 3526 5 Size: 47 2169 1305 2081 Fragments arranged by size: 2169 2081 1305 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5602 StyI C'CwwG_G Cuts at: 513 1065 2422 3041 513 Size: 552 1357 619 3074 Fragments arranged by size: 3074 1357 619 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2179 2257 3629 3646 3799 3984 4429 Size: 1255 599 78 1372 17 153 185 445 Cuts at: 4429 325 Size: 1498 Fragments arranged by size: 1498 1372 1255 599 445 185 153 78 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3848 5560 96 Size: 274 80 33 51 1443 1871 1712 138 Fragments arranged by size: 1871 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5562 5594 5562 Size: 32 5570 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5602 XbaI T'CTAG_A Cuts at: 2938 2938 Size: 5602 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 5602 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5602 XmaI C'CCGG_G Cuts at: 1870 2877 2927 1870 Size: 1007 50 4545 Fragments arranged by size: 4545 1007 50 XmnI GAAnn'nnTTC Cuts at: 2148 3731 2148 Size: 1583 4019 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3410 276 Size: 53 83 186 936 1876 2468 Fragments arranged by size: 2468 1876 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI