(Circular) (Six-base) MAPSORT of: pFN21AB0482.seq Check: 4619 from: 1 to: 7777 pFN21AB0482 7777 bp With 182 enzymes: SgfI * August 16, 2010 14:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3386 5587 278 Size: 53 83 186 936 1850 2201 2468 Fragments arranged by size: 2468 2201 1850 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5120 1937 Size: 3183 4594 Acc65I G'GTAC_C Cuts at: 5098 5098 Size: 7777 AclI AA'CG_TT Cuts at: 1563 5904 6277 1563 Size: 4341 373 3063 Fragments arranged by size: 4341 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2471 2735 2979 4377 4394 5840 7224 7492 Size: 839 264 244 1398 17 1446 1384 268 Cuts at: 7492 1632 Size: 1917 Fragments arranged by size: 1917 1446 1398 1384 839 268 264 244 17 AflII C'TTAA_G Cuts at: 820 1017 2222 4895 820 Size: 197 1205 2673 3702 Fragments arranged by size: 3702 2673 1205 197 AflIII A'CryG_T Cuts at: 1203 6676 7653 1203 Size: 5473 977 1327 Fragments arranged by size: 5473 1327 977 AgeI A'CCGG_T Cuts at: 6608 6608 Size: 7777 AhdI GACnn_n'nnGTC Cuts at: 6506 6506 Size: 7777 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7777 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2169 2565 2892 1184 Size: 985 396 327 6069 Fragments arranged by size: 6069 985 396 327 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3191 3223 3191 Size: 32 7745 AlwNI CAG_nnn'CTG Cuts at: 2414 4134 5520 7092 2414 Size: 1720 1386 1572 3099 Fragments arranged by size: 3099 1720 1572 1386 ApaI G_GGCC'C Cuts at: 2629 2629 Size: 7777 ApaLI G'TGCA_C Cuts at: 5834 6990 5834 Size: 1156 6621 ApoI r'AATT_y Cuts at: 1493 2008 2597 2759 2976 3323 3636 3725 3977 Size: 515 589 162 217 347 313 89 252 Cuts at: 3977 4476 4779 4831 4931 5086 5312 5464 7573 Size: 499 303 52 100 155 226 152 2109 Cuts at: 7573 1493 Size: 1697 Fragments arranged by size: 2109 1697 589 515 499 347 313 303 252 226 217 162 155 152 100 89 52 AseI AT'TA_AT Cuts at: 160 1032 6331 160 Size: 872 5299 1606 Fragments arranged by size: 5299 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7777 AvaI C'yCGr_G Cuts at: 1870 1943 5102 1870 Size: 73 3159 4545 Fragments arranged by size: 4545 3159 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3472 3505 3472 Size: 33 7744 BamHI G'GATC_C Cuts at: 5107 5474 5107 Size: 367 7410 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2876 5098 6553 618 Size: 325 217 627 1089 2222 1455 1842 Fragments arranged by size: 2222 1842 1455 1089 627 325 217 BanII G_rGCy'C Cuts at: 1391 2035 2495 2567 2629 3282 5096 1391 Size: 644 460 72 62 653 1814 4072 Fragments arranged by size: 4072 1814 653 644 460 72 62 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2596 2916 3017 3370 3897 928 Size: 971 697 320 101 353 527 4808 Fragments arranged by size: 4808 971 697 527 353 320 101 BbvCI CC'TCA_GC Cuts at: 2889 4127 2889 Size: 1238 6539 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5968 6002 1903 Size: 34 4031 34 3678 Fragments arranged by size: 4031 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5669 6879 5669 Size: 1210 6567 BclI T'GATC_A Cuts at: 1548 2656 2943 1548 Size: 1108 287 6382 Fragments arranged by size: 6382 1108 287 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3512 6388 136 Size: 107 122 71 631 605 1840 2876 1525 Fragments arranged by size: 2876 1840 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7772 7772 Size: 7777 BlpI GC'TnA_GC Cuts at: 5194 5194 Size: 7777 Bme1580I G_kGCm'C Cuts at: 1790 2629 2881 3829 5838 6994 1790 Size: 839 252 948 2009 1156 2573 Fragments arranged by size: 2573 2009 1156 948 839 252 BmgBI CAC'GTC Cuts at: 1318 4678 1318 Size: 3360 4417 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3285 5508 6466 Size: 416 53 83 399 24 1868 2223 958 Cuts at: 6466 442 Size: 1753 Fragments arranged by size: 2223 1868 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7777 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3085 3085 Size: 7777 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3218 3610 4010 4774 6437 1239 Size: 1979 392 400 764 1663 2579 Fragments arranged by size: 2579 1979 1663 764 400 392 Bpu10I CC'TnA_GC Cuts at: 2310 2889 3716 4127 4691 2310 Size: 579 827 411 564 5396 Fragments arranged by size: 5396 827 579 564 411 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3016 4246 5902 6767 7065 3016 Size: 1230 1656 865 298 3728 Fragments arranged by size: 3728 1656 1230 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3180 6440 882 Size: 617 1681 3260 2219 Fragments arranged by size: 3260 2219 1681 617 BsaAI yAC'GTr Cuts at: 493 1204 5568 493 Size: 711 4364 2702 Fragments arranged by size: 4364 2702 711 BsaBI GATnn'nnATC Cuts at: 5034 7771 5034 Size: 2737 5040 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3383 5584 5966 6601 Size: 53 83 186 936 1850 2201 382 635 Cuts at: 6601 275 Size: 1451 Fragments arranged by size: 2201 1850 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2567 3477 6210 6608 6882 7029 7440 2567 Size: 910 2733 398 274 147 411 2904 Fragments arranged by size: 2904 2733 910 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3191 3221 1178 Size: 30 165 30 1788 30 5734 Fragments arranged by size: 5734 1788 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3272 3413 4730 1186 Size: 2086 141 1317 4233 Fragments arranged by size: 4233 2086 1317 141 BseYI C'CCAG_C Cuts at: 3938 6980 7476 3938 Size: 3042 496 4239 Fragments arranged by size: 4239 3042 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 7204 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5227 5988 7016 1540 Size: 275 186 3226 761 1028 2301 Fragments arranged by size: 3226 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5838 5923 6994 5838 Size: 85 1071 6621 Fragments arranged by size: 6621 1071 85 BsmI GAATG_Cn' Cuts at: 4557 5288 5381 4557 Size: 731 93 6953 Fragments arranged by size: 6953 731 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2621 3390 2621 Size: 769 7008 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2035 2495 2567 2629 2881 3282 3829 Size: 399 245 460 72 62 252 401 547 Cuts at: 3829 5096 5838 5923 6994 1391 Size: 1267 742 85 1071 2174 Fragments arranged by size: 2174 1267 1071 742 547 460 401 399 252 245 85 72 62 BspEI T'CCGG_A Cuts at: 2567 7440 2567 Size: 4873 2904 BspHI T'CATG_A Cuts at: 3006 3388 4234 4609 5666 7396 3006 Size: 382 846 375 1057 1730 3387 Fragments arranged by size: 3387 1730 1057 846 382 375 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5132 844 Size: 4288 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5664 1394 Size: 429 3841 3507 Fragments arranged by size: 3841 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6272 6446 65 Size: 1183 5024 174 1396 Fragments arranged by size: 5024 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6421 6608 1700 Size: 110 4611 187 2869 Fragments arranged by size: 4611 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7777 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7777 BssSI C'ACGA_G Cuts at: 5837 6849 5837 Size: 1012 6765 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7777 BstBI TT'CG_AA Cuts at: 3228 6581 3228 Size: 3353 4424 BstEII G'GTnAC_C Cuts at: 1184 3403 1184 Size: 2219 5558 BstYI r'GATC_y Cuts at: 1972 5107 5474 5859 5876 7328 7414 7426 7772 Size: 3135 367 385 17 1452 86 12 346 Cuts at: 7772 1972 Size: 1977 Fragments arranged by size: 3135 1977 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2855 4073 2855 Size: 1218 6559 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 7225 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3556 487 Size: 683 56 2330 4708 Fragments arranged by size: 4708 2330 683 56 BtsI GCAGTG_nn' Cuts at: 3543 4518 4861 5293 6087 6107 3543 Size: 975 343 432 794 20 5213 Fragments arranged by size: 5213 975 794 432 343 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7777 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7742 DraI TTT'AAA Cuts at: 2319 5062 5081 2319 Size: 2743 19 5015 Fragments arranged by size: 5015 2743 19 DraIII CAC_nnn'GTG Cuts at: 2449 2850 2449 Size: 401 7376 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4405 6784 809 Size: 507 3089 2379 1802 Fragments arranged by size: 3089 2379 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2703 5224 6113 Size: 54 1425 50 275 18 873 2521 889 Cuts at: 6113 7742 8 Size: 1629 43 Fragments arranged by size: 2521 1629 1425 889 873 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5224 1537 Size: 275 3412 4090 Fragments arranged by size: 4090 3412 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2490 3909 4611 5707 1353 Size: 1137 1419 702 1096 3423 Fragments arranged by size: 3423 1419 1137 1096 702 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6364 6738 6884 387 Size: 1064 138 4775 374 146 1280 Fragments arranged by size: 4775 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2471 2735 2979 3218 3610 4010 4377 Size: 393 839 264 244 239 392 400 367 Cuts at: 4377 4394 4774 5840 6437 7224 7492 1239 Size: 17 380 1066 597 787 268 1524 Fragments arranged by size: 1524 1066 839 787 597 400 393 392 380 367 268 264 244 239 17 EcoNI CCTnn'n_nnAGG Cuts at: 2477 2798 2477 Size: 321 7456 EcoO109I rG'GnC_Cy Cuts at: 2625 3919 4720 2625 Size: 1294 801 5682 Fragments arranged by size: 5682 1294 801 EcoRI G'AATT_C Cuts at: 2759 3977 5086 5464 2759 Size: 1218 1109 378 5072 Fragments arranged by size: 5072 1218 1109 378 EcoRV GAT'ATC Cuts at: 4033 4040 4033 Size: 7 7770 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5024 5024 Size: 7777 FspI TGC'GCA Cuts at: 6283 6283 Size: 7777 HaeII r_GCGC'y Cuts at: 1678 2919 6924 1678 Size: 1241 4005 2531 Fragments arranged by size: 4005 2531 1241 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3351 3383 3440 3472 3555 Size: 32 135 32 1779 32 57 32 83 Cuts at: 3555 3587 3686 3718 6418 6450 6492 6524 1373 Size: 32 99 32 2700 32 42 32 2626 Fragments arranged by size: 2700 2626 1779 135 99 83 57 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4986 5121 5367 669 Size: 988 281 3048 135 246 3079 Fragments arranged by size: 3079 3048 988 281 246 135 HindIII A'AGCT_T Cuts at: 748 3732 3751 4736 748 Size: 2984 19 985 3789 Fragments arranged by size: 3789 2984 985 19 HpaI GTT'AAC Cuts at: 4986 5367 4986 Size: 381 7396 KpnI G_GTAC'C Cuts at: 5102 5102 Size: 7777 MfeI C'AATT_G Cuts at: 3814 5376 3814 Size: 1562 6215 MluI A'CGCG_T Cuts at: 7653 7653 Size: 7777 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6891 7075 1305 Size: 5586 184 2007 Fragments arranged by size: 5586 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2705 10 Size: 54 1425 343 873 5082 Fragments arranged by size: 5082 1425 873 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2171 2705 3141 4325 5735 6094 6253 Size: 648 1005 534 436 1184 1410 359 159 Cuts at: 6253 518 Size: 2042 Fragments arranged by size: 2042 1410 1184 1005 648 534 436 359 159 MspA1I CmG'CkG Cuts at: 1606 2458 5193 5870 7018 7263 1606 Size: 852 2735 677 1148 245 2120 Fragments arranged by size: 2735 2120 1148 852 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7225 NdeI CA'TA_TG Cuts at: 387 387 Size: 7777 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7777 NotI GC'GGCC_GC Cuts at: 5224 5224 Size: 7777 NruI TCG'CGA Cuts at: 2109 2109 Size: 7777 NspI r_CATG'y Cuts at: 1132 1229 5135 6680 1132 Size: 97 3906 1545 2229 Fragments arranged by size: 3906 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2629 4665 1348 Size: 1281 2036 4460 Fragments arranged by size: 4460 2036 1281 PciI A'CATG_T Cuts at: 6676 6676 Size: 7777 PflMI CCAn_nnn'nTGG Cuts at: 1108 2559 1108 Size: 1451 6326 PfoI T'CCnGG_A Cuts at: 2664 3342 4002 2664 Size: 678 660 6439 Fragments arranged by size: 6439 678 660 PmeI GTTT'AAAC Cuts at: 5081 5081 Size: 7777 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3191 3223 5846 5878 7359 7391 3191 Size: 32 2623 32 1481 32 3577 Fragments arranged by size: 3577 2623 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 4720 4720 Size: 7777 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7777 PsiI TTA'TAA Cuts at: 2063 5347 2063 Size: 3284 4493 PspOMI G'GGCC_C Cuts at: 2625 2625 Size: 7777 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2082 2114 2082 Size: 32 7745 PstI C_TGCA'G Cuts at: 830 2481 2544 3237 3889 5129 830 Size: 1651 63 693 652 1240 3478 Fragments arranged by size: 3478 1651 1240 693 652 63 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7777 PvuII CAG'CTG Cuts at: 2458 2458 Size: 7777 SalI G'TCGA_C Cuts at: 1936 5119 1936 Size: 3183 4594 SapI GCTCTTCn'nnn_ Cuts at: 2490 4611 2490 Size: 2121 5656 SbfI CC_TGCA'GG Cuts at: 2481 5129 2481 Size: 2648 5129 ScaI AGT'ACT Cuts at: 4924 6025 7737 4924 Size: 1101 1712 4964 Fragments arranged by size: 4964 1712 1101 SfcI C'TryA_G Cuts at: 826 1045 2477 2540 3233 3885 4241 5125 6260 Size: 219 1432 63 693 652 356 884 1135 Cuts at: 6260 6941 7132 826 Size: 681 191 1471 Fragments arranged by size: 1471 1432 1135 884 693 681 652 356 219 191 63 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7777 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7777 SmaI CCC'GGG Cuts at: 1872 5104 1872 Size: 3232 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2222 2995 4225 4895 5881 6782 Size: 197 926 279 773 1230 670 986 901 Cuts at: 6782 7044 820 Size: 262 1553 Fragments arranged by size: 1553 1230 986 926 901 773 670 279 262 197 SnaBI TAC'GTA Cuts at: 493 5568 493 Size: 5075 2702 SpeI A'CTAG_T Cuts at: 152 5065 152 Size: 4913 2864 SphI G_CATG'C Cuts at: 1132 5135 1132 Size: 4003 3774 SspI AAT'ATT Cuts at: 5 52 4053 5701 5 Size: 47 4001 1648 2081 Fragments arranged by size: 4001 2081 1648 47 StuI AGG'CCT Cuts at: 1517 3583 4362 1517 Size: 2066 779 4932 Fragments arranged by size: 4932 2066 779 StyI C'CwwG_G Cuts at: 513 1065 2362 3408 5216 513 Size: 552 1297 1046 1808 3074 Fragments arranged by size: 3074 1808 1297 1046 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3041 4868 5004 5804 5821 5974 6159 Size: 1255 1461 1827 136 800 17 153 185 Cuts at: 6159 6604 325 Size: 445 1498 Fragments arranged by size: 1827 1498 1461 1255 800 445 185 153 136 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3348 3698 4922 Size: 274 80 33 51 1443 1371 350 1224 Cuts at: 4922 6023 7735 96 Size: 1101 1712 138 Fragments arranged by size: 1712 1443 1371 1224 1101 350 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4833 4865 7737 7769 4833 Size: 32 2872 32 4841 Fragments arranged by size: 4841 2872 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2265 1316 Size: 949 6828 XbaI T'CTAG_A Cuts at: 5113 5113 Size: 7777 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7777 XmaI C'CCGG_G Cuts at: 1870 5102 1870 Size: 3232 4545 XmnI GAAnn'nnTTC Cuts at: 4052 4476 4605 4817 5906 4052 Size: 424 129 212 1089 5923 Fragments arranged by size: 5923 1089 424 212 129 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3384 5585 276 Size: 53 83 186 936 1850 2201 2468 Fragments arranged by size: 2468 2201 1850 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BbeI BfrBI BsiWI BstXI BstZ17I EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PmlI PspXI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI