################################## # NO CUT ENZYMES ( 173 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BbvI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DdeI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FokI FseI HaeII HgaI Hin1I HincII HindII HindIII HinfI HpaI KasI KpnI Ksp632I MaeI MaeII MamI MfeI MluI MluNI MroI MscI MseI MslI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PflFI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfaNI SfcI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I TseI Tsp509I Tth111I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AfaI 76 GTAC GT^AC AlwI 99 GGATC GGATC(4/5) AlwNI 67 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 399 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 247 CTCGAG C^[CT]CG[GA]G AviII 233 TGCGCA TGC^GCA BcnI 6 CCGGG CC^[GC]GG BsiYI 216 CCAAGAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 216 CCAAGAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsoBI 247 CTCGAG C^[CT]CG[GA]G BspMI 66 GCAGGT ACCTGC(4/8) BsrFI 126 GCCGGC [GA]^CCGG[CT] BstYI 99 GGATCT [GA]^GATC[CT] Cfr10I 126 GCCGGC [GA]^CCGG[CT] DpnII 100 GATC ^GATC DsaI 167 CCGCGG C^C[GA][CT]GG EcoT14I 374 CCAAGG C^C[AT][AT]GG FspI 233 TGCGCA TGC^GCA HphI 183 TCACC GGTGA(8/7) KspI 167 CCGCGG CCGC^GG MboI 100 GATC ^GATC MflI 99 GGATCT [GA]^GATC[CT] MspA1I 167 CCGCGG C[AC]G^C[GT]G NaeI 126 GCCGGC GCC^GGC NciI 6 CCGGG CC^[GC]GG NdeII 100 GATC ^GATC NgoMIV 126 GCCGGC G^CCGGC PaeR7I 247 CTCGAG C^TCGAG PflMI 216 CCAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 68 AGGTCCT [GA]G^G[AT]CC[CT] RsaI 76 GTAC GT^AC SacII 167 CCGCGG CCGC^GG Sau3AI 100 GATC ^GATC SfiI 162 GGCCACCGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SphI 230 GCATGC GCATG^C StyI 374 CCAAGG C^C[AT][AT]GG TaqI 248 TCGA T^CGA Tsp45I 155 GTCAC ^GT[GC]AC TspRI 137 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 248 TCGA T^CGA Van91I 216 CCAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 374 CCAAGGAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 247 CTCGAG C^TCGAG XhoII 99 GGATCT [GA]^GATC[CT] XmnI 399 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### BglI 49 GCCTCTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 163 GCCACCGCGGC BsaI 262 GAGACC GGTCTC(1/5) 286 GAGACC BsmAI 262 GAGAC GTCTC(1/5) 286 GAGAC BsrI 138 ACTGG ACTGG(1/-1) 390 CCAGT BstNI 72 CCTGG CC^[AT]GG 122 CCTGG DraII 68 AGGTCCT [GA]G^G[ACGT]CC[CT] 251 AGGCCCC EcoO109I 68 AGGTCCT [GA]G^G[ACGT]CC[CT] 251 AGGCCCC EcoRII 72 CCTGG ^CC[AT]GG 122 CCTGG FauI 195 CCCGC CCCGC(4/6) 130 GCGGG MaeIII 90 GTTAC ^GT[ACGT]AC 155 GTCAC MvaI 72 CCTGG CC^[AT]GG 122 CCTGG NlaIII 231 CATG CATG^ 315 CATG NlaIV 60 GGAGCC GG[ACGT]^[ACGT]CC 252 GGCCCC NspI 230 GCATGC [GA]CATG^[CT] 314 ACATGC SmlI 247 CTCGAG C^T[CT][GA]AG 325 CTTGAG ######################################### # 3 OR MORE CUT ENZYMES ( 25 enzymes) # ######################################### AccII AciI AluI AvaII BsaJI BssKI BstUI Cac8I CfoI Cfr13I EaeI Fnu4HI HaeIII HapII HhaI HinP1I HpaII ItaI MboII MnlI MspI MvnI MwoI Sau96I ScrFI