Restriction map for
pFN21AE5643
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BamHI BanII BbeI
BbrPI BbsI BbvCI BcgI BclI
BcnI BfaI BfrI BglI BlnI
BlpI BmyI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsiEI BsiHKAI BsiWI
BsmBI BsoBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I CelII CfoI Cfr10I
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRV FauI FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HpaI KasI
KpnI KspI MaeI MaeII MfeI
MluI MluNI MscI MunI MvnI
NaeI NarI NciI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PstI PvuI
PvuII RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccIII 1286 TCCGGA T^CCGGA
AflIII 763 ACATGT A^C[GA][CT]GT
AlwI 364 GGATC GGATC(4/5)
AlwNI 67 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 555 CCTGAGG CC^T[ACGT]AGG
AseI 799 ATTAAT AT^TAAT
AsnI 799 ATTAAT AT^TAAT
Asp700I 855 GAAATCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 1250 GGACC G^G[AT]CC
BanI 1391 GGCACC G^G[CT][GA]CC
BbvI 66 GCAGC GCAGC(8/12)
BciVI 448 GGATAC GTATCC(6/5)
BglII 960 AGATCT A^GATCT
BsaBI 631 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1286 TCCGGA [AT]^CCGG[AT]
BseAI 1286 TCCGGA T^CCGGA
BseRI 558 GAGGAG GAGGAG(10/8)
BsgI 236 GTGCAG GTGCAG(16/14)
BspDI 1051 ATCGAT AT^CGAT
BspEI 1286 TCCGGA T^CCGGA
BspLU11I 763 ACATGT A^CATGT
Bsu36I 555 CCTGAGG CC^T[ACGT]AGG
Cac8I 564 GCATGC GC[ACGT]^[ACGT]GC
ClaI 1051 ATCGAT AT^CGAT
DsaI 199 CCATGG C^C[GA][CT]GG
EaeI 231 TGGCCG [CT]^GGCC[GA]
Eco81I 555 CCTGAGG CC^T[ACGT]AGG
EcoRI 1403 GAATTC G^AATTC
EcoT14I 199 CCATGG C^C[AT][AT]GG
EcoT22I 730 ATGCAT ATGCA^T
HapII 1287 CCGG C^CGG
HindIII 625 AAGCTT A^AGCTT
HpaII 1287 CCGG C^CGG
MamI 631 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1286 TCCGGA T^CCGGA
MslI 592 CATAGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 67 CAGCGG C[AC]G^C[GT]G
MspI 1287 CCGG C^CGG
MwoI 333 GCTGACTACGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 199 CCATGG C^CATGG
NdeI 1014 CATATG CA^TATG
NsiI 730 ATGCAT ATGCA^T
PleI 294 GAGTC GAGTC(4/5)
PpuMI 1249 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 799 ATTAAT AT^TAAT
SapI 602 GAAGAGC GCTCTTC(1/4)
SfaNI 108 GATGC GCATC(5/9)
SmlI 174 CTTGAG C^T[CT][GA]AG
SphI 564 GCATGC GCATG^C
StuI 1025 AGGCCT AGG^CCT
StyI 199 CCATGG C^C[AT][AT]GG
TfiI 75 GAATC G^A[AT]TC
TseI 66 GCAGC G^C[AT]GC
XmnI 855 GAAATCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
BpmI 1410 CTCCAG CTGGAG(16/14)
112 CTGGAG
BsmAI 133 GTCTC GTCTC(1/5)
296 GTCTC
BsmI 1351 GAATGC GAATGC(1/-1)
607 GCATTC
Bsp1407I 95 TGTACA T^GTACA
420 TGTACA
BspHI 804 TCATGA T^CATGA
1056 TCATGA
BsrGI 95 TGTACA T^GTACA
420 TGTACA
BstYI 364 GGATCT [GA]^GATC[CT]
960 AGATCT
Cfr13I 662 GGCCC G^G[ACGT]CC
1250 GGACC
DraII 661 AGGCCCT [GA]G^G[ACGT]CC[CT]
1249 AGGACCT
EcoO109I 661 AGGCCCT [GA]G^G[ACGT]CC[CT]
1249 AGGACCT
Fnu4HI 66 GCAGC GC^[ACGT]GC
69 GCGGC
HinfI 75 GAATC G^A[ACGT]TC
294 GAGTC
HphI 50 TCACC GGTGA(8/7)
552 TCACC
ItaI 66 GCAGC GC^[ACGT]GC
69 GCGGC
MflI 364 GGATCT [GA]^GATC[CT]
960 AGATCT
MseI 800 TTAA T^TAA
1294 TTAA
NlaIV 195 GGAGCC GG[ACGT]^[ACGT]CC
1391 GGCACC
NspI 564 GCATGC [GA]CATG^[CT]
763 ACATGT
RcaI 804 TCATGA T^CATGA
1056 TCATGA
Sau96I 662 GGCCC G^G[ACGT]CC
1250 GGACC
SfcI 513 CTGTAG C^T[GA][CT]AG
1254 CTATAG
SspBI 95 TGTACA T^GTACA
420 TGTACA
TaqI 29 TCGA T^CGA
1052 TCGA
Tru9I 800 TTAA T^TAA
1294 TTAA
TthHB8I 29 TCGA T^CGA
1052 TCGA
XhoII 364 GGATCT [GA]^GATC[CT]
960 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BsaJI BsiYI BslI BsmFI BsrI
BssKI BstF5I BstNI DdeI DpnII
EarI EcoRII FokI HaeIII Ksp632I
MaeIII MboI MboII MnlI MvaI
NdeII NlaIII RsaI Sau3AI ScrFI
Tsp45I Tsp509I TspRI