(Circular) (Six-base) MAPSORT of: pFN21AE5643.seq Check: 3823 from: 1 to: 6121 pFN21AE5643 6121 bp With 182 enzymes: SgfI * December 8, 2014 13:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3931 278 Size: 53 83 186 936 2395 2468 Fragments arranged by size: 2468 2395 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3464 1937 Size: 1527 4594 Acc65I G'GTAC_C Cuts at: 3442 3442 Size: 6121 AclI AA'CG_TT Cuts at: 1563 4248 4621 1563 Size: 2685 373 3063 Fragments arranged by size: 3063 2685 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2851 4184 5568 5836 1632 Size: 1219 1333 1384 268 1917 Fragments arranged by size: 1917 1384 1333 1219 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5924 AflIII A'CryG_T Cuts at: 1203 2768 5020 5997 1203 Size: 1565 2252 977 1327 Fragments arranged by size: 2252 1565 1327 977 AgeI A'CCGG_T Cuts at: 4952 4952 Size: 6121 AhdI GACnn_n'nnGTC Cuts at: 4850 4850 Size: 6121 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6121 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6121 AlwNI CAG_nnn'CTG Cuts at: 2077 3864 5436 2077 Size: 1787 1572 2762 Fragments arranged by size: 2762 1787 1572 ApaLI G'TGCA_C Cuts at: 4178 5334 4178 Size: 1156 4965 ApoI r'AATT_y Cuts at: 1493 2486 2817 3069 3408 3430 3656 3808 5917 Size: 993 331 252 339 22 226 152 2109 Cuts at: 5917 1493 Size: 1697 Fragments arranged by size: 2109 1697 993 339 331 252 226 152 22 AseI AT'TA_AT Cuts at: 160 1032 2805 4675 160 Size: 872 1773 1870 1606 Fragments arranged by size: 1870 1773 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6121 AvaI C'yCGr_G Cuts at: 1870 1943 3446 1870 Size: 73 1503 4545 Fragments arranged by size: 4545 1503 73 BamHI G'GATC_C Cuts at: 3451 3818 3451 Size: 367 5754 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3396 3442 4897 618 Size: 325 217 627 1609 46 1455 1842 Fragments arranged by size: 1842 1609 1455 627 325 217 46 BanII G_rGCy'C Cuts at: 1391 3440 1391 Size: 2049 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5150 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4312 4346 1903 Size: 34 2375 34 3678 Fragments arranged by size: 3678 2375 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2447 4013 5223 2447 Size: 1566 1210 3345 Fragments arranged by size: 3345 1566 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6121 BfrBI ATG'CAT Cuts at: 2737 2737 Size: 6121 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4732 136 Size: 107 122 71 631 605 3060 1525 Fragments arranged by size: 3060 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2965 6116 2965 Size: 3151 2970 BlpI GC'TnA_GC Cuts at: 3538 3538 Size: 6121 Bme1580I G_kGCm'C Cuts at: 1790 4182 5338 1790 Size: 2392 1156 2573 Fragments arranged by size: 2573 2392 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6121 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2399 3087 3171 Size: 416 53 83 399 24 982 688 84 Cuts at: 3171 3852 4810 442 Size: 681 958 1753 Fragments arranged by size: 1753 982 958 688 681 416 399 84 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6121 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2138 3400 4781 1239 Size: 899 1262 1381 2579 Fragments arranged by size: 2579 1381 1262 899 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2200 4246 5111 5409 2200 Size: 2046 865 298 2912 Fragments arranged by size: 2912 2046 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4784 882 Size: 617 3285 2219 Fragments arranged by size: 3285 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3912 493 Size: 711 2708 2702 Fragments arranged by size: 2708 2702 711 BsaBI GATnn'nnATC Cuts at: 2640 6115 2640 Size: 3475 2646 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3928 4310 4945 275 Size: 53 83 186 936 2395 382 635 1451 Fragments arranged by size: 2395 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3291 4554 4952 5226 5373 5784 3291 Size: 1263 398 274 147 411 3628 Fragments arranged by size: 3628 1263 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2192 2222 1178 Size: 30 165 30 789 30 5077 Fragments arranged by size: 5077 789 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2578 1186 Size: 1392 4729 BseYI C'CCAG_C Cuts at: 2227 5324 5820 2227 Size: 3097 496 2528 Fragments arranged by size: 3097 2528 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2262 1163 Size: 573 526 5022 Fragments arranged by size: 5022 573 526 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3571 4332 5360 1540 Size: 275 186 1570 761 1028 2301 Fragments arranged by size: 2301 1570 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4182 4267 5338 4182 Size: 85 1071 4965 Fragments arranged by size: 4965 1071 85 BsmI GAATG_Cn' Cuts at: 2612 3362 3632 3725 2612 Size: 750 270 93 5008 Fragments arranged by size: 5008 750 270 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3440 4182 4267 5338 1391 Size: 399 1650 742 85 1071 2174 Fragments arranged by size: 2174 1650 1071 742 399 85 BspEI T'CCGG_A Cuts at: 3291 5784 3291 Size: 2493 3628 BspHI T'CATG_A Cuts at: 2809 3061 4010 5740 2809 Size: 252 949 1730 3190 Fragments arranged by size: 3190 1730 949 252 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3476 844 Size: 2632 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4008 1394 Size: 429 2185 3507 Fragments arranged by size: 3507 2185 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4616 4790 65 Size: 1183 3368 174 1396 Fragments arranged by size: 3368 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4765 4952 1700 Size: 110 2955 187 2869 Fragments arranged by size: 2955 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2100 2425 96 Size: 2004 325 3792 Fragments arranged by size: 3792 2004 325 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6121 BssSI C'ACGA_G Cuts at: 4181 5193 4181 Size: 1012 5109 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6121 BstBI TT'CG_AA Cuts at: 4925 4925 Size: 6121 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6121 BstYI r'GATC_y Cuts at: 1972 2369 2965 3451 3818 4203 4220 5672 5758 Size: 397 596 486 367 385 17 1452 86 Cuts at: 5758 5770 6116 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 596 486 397 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2561 2561 Size: 6121 BtgI C'CryG_G Cuts at: 513 1065 2204 513 Size: 552 1139 4430 Fragments arranged by size: 4430 1139 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5382 Fragments arranged by size: 5382 683 56 BtsI GCAGTG_nn' Cuts at: 3637 4431 4451 3637 Size: 794 20 5307 Fragments arranged by size: 5307 794 20 ClaI AT'CG_AT Cuts at: 1554 3057 1554 Size: 1503 4618 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6086 DraI TTT'AAA Cuts at: 3425 3425 Size: 6121 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5128 809 Size: 507 3812 1802 Fragments arranged by size: 3812 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2236 3568 4457 Size: 54 1425 50 275 18 406 1332 889 Cuts at: 4457 6086 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1332 889 406 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3568 1537 Size: 275 1756 4090 Fragments arranged by size: 4090 1756 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2017 2211 2602 3077 4051 1353 Size: 664 194 391 475 974 3423 Fragments arranged by size: 3423 974 664 475 391 194 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4708 5082 5228 387 Size: 1064 138 3119 374 146 1280 Fragments arranged by size: 3119 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2138 2851 3400 4184 4781 5568 5836 Size: 393 506 713 549 784 597 787 268 Cuts at: 5836 1239 Size: 1524 Fragments arranged by size: 1524 787 784 713 597 549 506 393 268 EcoO109I rG'GnC_Cy Cuts at: 2667 3255 2667 Size: 588 5533 EcoRI G'AATT_C Cuts at: 3408 3430 3808 3408 Size: 22 378 5721 Fragments arranged by size: 5721 378 22 FspI TGC'GCA Cuts at: 4627 4627 Size: 6121 HaeII r_GCGC'y Cuts at: 1678 5268 1678 Size: 3590 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2390 2422 4762 4794 4836 Size: 32 135 32 818 32 2340 32 42 Cuts at: 4836 4868 1373 Size: 32 2626 Fragments arranged by size: 2626 2340 818 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3465 3711 669 Size: 988 281 1527 246 3079 Fragments arranged by size: 3079 1527 988 281 246 HindIII A'AGCT_T Cuts at: 748 2630 748 Size: 1882 4239 HpaI GTT'AAC Cuts at: 3711 3711 Size: 6121 KpnI G_GTAC'C Cuts at: 3446 3446 Size: 6121 MfeI C'AATT_G Cuts at: 3720 3720 Size: 6121 MluI A'CGCG_T Cuts at: 5997 5997 Size: 6121 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2166 2190 5235 5419 1305 Size: 861 24 3045 184 2007 Fragments arranged by size: 3045 2007 861 184 24 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4299 Fragments arranged by size: 4299 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2601 4079 4438 4597 518 Size: 648 1435 1478 359 159 2042 Fragments arranged by size: 2042 1478 1435 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2074 3537 4214 5362 5607 1606 Size: 468 1463 677 1148 245 2120 Fragments arranged by size: 2120 1463 1148 677 468 245 NcoI C'CATG_G Cuts at: 513 1065 2204 513 Size: 552 1139 4430 Fragments arranged by size: 4430 1139 552 NdeI CA'TA_TG Cuts at: 387 3020 387 Size: 2633 3488 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6121 NotI GC'GGCC_GC Cuts at: 3568 3568 Size: 6121 NsiI A_TGCA'T Cuts at: 2739 2739 Size: 6121 NspI r_CATG'y Cuts at: 1132 1229 2573 2772 3479 5024 1132 Size: 97 1344 199 707 1545 2229 Fragments arranged by size: 2229 1545 1344 707 199 97 PasI CC'CwG_GG Cuts at: 1348 2017 2059 1348 Size: 669 42 5410 Fragments arranged by size: 5410 669 42 PciI A'CATG_T Cuts at: 2768 5020 2768 Size: 2252 3869 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6121 PfoI T'CCnGG_A Cuts at: 2786 2786 Size: 6121 PmeI GTTT'AAAC Cuts at: 3425 3425 Size: 6121 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4190 4222 5703 5735 4190 Size: 32 1481 32 4576 Fragments arranged by size: 4576 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3255 3255 Size: 6121 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6121 PsiI TTA'TAA Cuts at: 3691 3691 Size: 6121 PstI C_TGCA'G Cuts at: 830 3473 830 Size: 2643 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6121 SalI G'TCGA_C Cuts at: 1936 3463 1936 Size: 1527 4594 SapI GCTCTTCn'nnn_ Cuts at: 2602 2602 Size: 6121 SbfI CC_TGCA'GG Cuts at: 3473 3473 Size: 6121 ScaI AGT'ACT Cuts at: 4369 6081 4369 Size: 1712 4409 SfcI C'TryA_G Cuts at: 826 1045 2518 3259 3469 4604 5285 5476 826 Size: 219 1473 741 210 1135 681 191 1471 Fragments arranged by size: 1473 1471 1135 741 681 219 210 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6121 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6121 SmaI CCC'GGG Cuts at: 1872 3448 1872 Size: 1576 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2179 4225 5126 5388 820 Size: 197 926 236 2046 901 262 1553 Fragments arranged by size: 2046 1553 926 901 262 236 197 SnaBI TAC'GTA Cuts at: 493 3912 493 Size: 3419 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6121 SphI G_CATG'C Cuts at: 1132 2573 3479 1132 Size: 1441 906 3774 Fragments arranged by size: 3774 1441 906 SspI AAT'ATT Cuts at: 5 52 4045 5 Size: 47 3993 2081 Fragments arranged by size: 3993 2081 47 StuI AGG'CCT Cuts at: 1517 3032 1517 Size: 1515 4606 StyI C'CwwG_G Cuts at: 513 1065 2204 3560 513 Size: 552 1139 1356 3074 Fragments arranged by size: 3074 1356 1139 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2072 4148 4165 4318 4503 4948 325 Size: 1255 492 2076 17 153 185 445 1498 Fragments arranged by size: 2076 1498 1255 492 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2100 2425 4367 Size: 274 80 33 51 1443 123 325 1942 Cuts at: 4367 6079 96 Size: 1712 138 Fragments arranged by size: 1942 1712 1443 325 274 138 123 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6081 6113 6081 Size: 32 6089 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6121 XbaI T'CTAG_A Cuts at: 3457 3457 Size: 6121 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6121 XmaI C'CCGG_G Cuts at: 1870 3446 1870 Size: 1576 4545 XmnI GAAnn'nnTTC Cuts at: 2864 4250 2864 Size: 1386 4735 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3929 276 Size: 53 83 186 936 2395 2468 Fragments arranged by size: 2468 2395 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI