################################## # NO CUT ENZYMES ( 133 enzymes) # ################################## AatII Acc65I AccI AclI AflII AflIII AgeI Alw44I AlwNI AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AviII AvrII BalI BamHI BbeI BbrPI BbsI BcgI BciVI BclI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsiEI BsiWI BsmI Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI KspI MaeII MaeIII MamI MfeI MluI MluNI MscI MseI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I Tsp45I XbaI XcmI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccIII 141 TCCGGA T^CCGGA AcsI 283 AAATTC [GA]^AATT[CT] AcyI 532 GACGCC G[GA]^CG[CT]C AhdI 391 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 564 GATCC GGATC(4/5) ApaI 337 GGGCCC GGGCC^C ApoI 283 AAATTC [GA]^AATT[CT] AspEI 391 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 610 GAGCAC G[AT]GC[AT]^C AspI 538 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC BbvCI 414 CCTCAGC CCTCAGC(-5/-2) BsaHI 532 GACGCC G[GA]^CG[CT]C BsaI 43 GAGACC GGTCTC(1/5) BseAI 141 TCCGGA T^CCGGA BsiHKAI 610 GAGCAC G[AT]GC[AT]^C BsmBI 769 GAGACG CGTCTC(1/5) BspEI 141 TCCGGA T^CCGGA BsrFI 551 GCCGGT [GA]^CCGG[CT] BsrI 443 CCAGT ACTGG(1/-1) BstXI 341 CCAGGCGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 551 GCCGGT [GA]^CCGG[CT] DraIII 616 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 689 TGGCCG [CT]^GGCC[GA] Eam1105I 391 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Hin1I 532 GACGCC G[GA]^CG[CT]C HinfI 397 GAGTC G^A[ACGT]TC HphI 821 TCACC GGTGA(8/7) MroI 141 TCCGGA T^CCGGA NspI 593 GCATGT [GA]CATG^[CT] PaeR7I 730 CTCGAG C^TCGAG PflFI 538 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 615 CCACCGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 397 GAGTC GAGTC(4/5) PstI 223 CTGCAG CTGCA^G SapI 798 GAAGAGC GCTCTTC(1/4) ScaI 734 AGTACT AGT^ACT SfaNI 233 GCATC GCATC(5/9) SfcI 223 CTGCAG C^T[GA][CT]AG SmaI 509 CCCGGG CCC^GGG StuI 383 AGGCCT AGG^CCT Tsp509I 284 AATT ^AATT Tth111I 538 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 615 CCACCGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 730 CTCGAG C^TCGAG XmaCI 509 CCCGGG C^CCGGG XmaI 509 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccII 599 CGCG CG^CG 619 CGCG AfaI 555 GTAC GT^AC 735 GTAC BanII 111 GAGCCC G[GA]GC[CT]^C 337 GGGCCC BcnI 509 CCCGG CC^[GC]GG 510 CCGGG BfaI 85 CTAG C^TAG 479 CTAG BglII 53 AGATCT A^GATCT 95 AGATCT BpmI 205 CTGGAG CTGGAG(16/14) 244 CTGGAG BsaWI 8 ACCGGA [AT]^CCGG[AT] 141 TCCGGA BsgI 193 CTGCAC GTGCAG(16/14) 784 GTGCAG BsmAI 43 GAGAC GTCTC(1/5) 769 GAGAC BsmFI 257 GGGAC GGGAC(10/14) 356 GGGAC BstUI 599 CGCG CG^CG 619 CGCG DraII 239 AGGACCT [GA]G^G[ACGT]CC[CT] 542 GGGTCCT DsaI 363 CCGTGG C^C[GA][CT]GG 635 CCGTGG EcoO109I 239 AGGACCT [GA]G^G[ACGT]CC[CT] 542 GGGTCCT EcoT14I 503 CCAAGG C^C[AT][AT]GG 716 CCAAGG FauI 740 CCCGC CCCGC(4/6) 600 GCGGG MaeI 85 CTAG C^TAG 479 CTAG MvnI 599 CGCG CG^CG 619 CGCG NciI 509 CCCGG CC^[GC]GG 510 CCGGG NlaIII 198 CATG CATG^ 594 CATG PpuMI 239 AGGACCT [GA]G^G[AT]CC[CT] 542 GGGTCCT PvuII 190 CAGCTG CAG^CTG 274 CAGCTG RsaI 555 GTAC GT^AC 735 GTAC StyI 503 CCAAGG C^C[AT][AT]GG 716 CCAAGG TspRI 319 CACTG CA[GC]TG[ACGT][ACGT]^ 444 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 53 enzymes) # ######################################### AciI AluI AvaI AvaII BanI BbvI BmyI BsaJI BseRI BsiYI BslI BsoBI Bsp1286I BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII EarI EcoRII Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HpaII ItaI Ksp632I MboI MboII MflI MnlI MspA1I MspI MvaI MwoI NdeII NlaIV Sau3AI Sau96I ScrFI SmlI TaqI TseI TthHB8I XhoII