################################## # NO CUT ENZYMES ( 125 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI Alw44I AlwNI Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BbrPI BbsI BciVI BclI BfaI BfrI BlnI BpuAI BsaBI BsaI BsaWI BseAI BsiWI BsmAI BsmBI BsmI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BssHII BssSI Bst1107I BstAPI BstBI BstXI BstZ17I ClaI CpoI DraI DraIII EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HpaI KpnI Ksp632I KspI MaeI MamI MfeI MluI MroI MseI MslI MunI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfuI SnaBI SpeI Sse8387I SspI StuI SwaI TfiI Tru9I Tsp509I Tth111I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccII 607 CGCG CG^CG AflIII 330 ACACGT A^C[GA][CT]GT AocI 148 CCTGAGG CC^T[ACGT]AGG Asp700I 201 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 292 GTGCTC G[AT]GC[AT]^C BbvCI 571 GCTGAGG CCTCAGC(-5/-2) BbvI 654 GCTGC GCAGC(8/12) BcgI 423 CGAGGAGTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 499 GCCGGTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 313 GCTGAGC GC^T[ACGT]AGC Bpu1102I 313 GCTGAGC GC^T[ACGT]AGC BsaAI 331 CACGTA [CT]AC^GT[GA] BsgI 380 CTGCAC GTGCAG(16/14) BsiEI 501 CGGTCG CG[GA][CT]^CG BsiHKAI 292 GTGCTC G[AT]GC[AT]^C Bsp1407I 47 TGTACA T^GTACA BsrGI 47 TGTACA T^GTACA BstEII 132 GGTCACC G^GT[ACGT]ACC BstPI 132 GGTCACC G^GT[ACGT]ACC BstUI 607 CGCG CG^CG Bsu36I 148 CCTGAGG CC^T[ACGT]AGG CelII 313 GCTGAGC GC^T[ACGT]AGC DrdI 124 GACCTCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 148 CCTGAGG CC^T[ACGT]AGG EcoO65I 132 GGTCACC G^GT[ACGT]ACC HgaI 284 GCGTC GACGC(5/10) HincII 286 GTCAAC GT[CT]^[GA]AC HindII 286 GTCAAC GT[CT]^[GA]AC HindIII 527 AAGCTT A^AGCTT HinfI 376 GAGTC G^A[ACGT]TC MboII 248 TCTTC GAAGA(8/7) MspA1I 483 CAGCGG C[AC]G^C[GT]G MvnI 607 CGCG CG^CG NaeI 472 GCCGGC GCC^GGC NcoI 581 CCATGG C^CATGG NdeI 161 CATATG CA^TATG NgoMIV 472 GCCGGC G^CCGGC NspI 594 GCATGC [GA]CATG^[CT] PleI 376 GAGTC GAGTC(4/5) SgrAI 471 CGCCGGCG C[GA]^CCGG[CT]G SmaI 561 CCCGGG CCC^GGG SmlI 295 CTCAAG C^T[CT][GA]AG SphI 594 GCATGC GCATG^C SspBI 47 TGTACA T^GTACA TaqI 140 TCGA T^CGA TseI 654 GCTGC G^C[AT]GC TthHB8I 140 TCGA T^CGA XmaCI 561 CCCGGG C^CCGGG XmaI 561 CCCGGG C^CCGGG XmnI 201 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AcyI 265 GGCGCC G[GA]^CG[CT]C 469 GGCGCC AfaI 48 GTAC GT^AC 334 GTAC AlwI 360 GATCC GGATC(4/5) 459 GATCC BalI 512 TGGCCA TGG^CCA 584 TGGCCA BanI 265 GGCGCC G^G[CT][GA]CC 469 GGCGCC BbeI 265 GGCGCC GGCGC^C 469 GGCGCC BcnI 561 CCCGG CC^[GC]GG 562 CCGGG BglII 245 AGATCT A^GATCT 278 AGATCT BpmI 127 CTCCAG CTGGAG(16/14) 167 CTGGAG BsaHI 265 GGCGCC G[GA]^CG[CT]C 469 GGCGCC BsrFI 472 GCCGGC [GA]^CCGG[CT] 499 GCCGGT BsrI 305 ACTGG ACTGG(1/-1) 515 CCAGT BstF5I 36 CATCC GGATG(2/0) 324 CATCC Cfr10I 472 GCCGGC [GA]^CCGG[CT] 499 GCCGGT DsaI 462 CCACGG C^C[GA][CT]GG 581 CCATGG EcoT14I 224 CCAAGG C^C[AT][AT]GG 581 CCATGG FokI 36 CATCC GGATG(9/13) 324 CATCC Hin1I 265 GGCGCC G[GA]^CG[CT]C 469 GGCGCC KasI 265 GGCGCC G^GCGCC 469 GGCGCC MaeIII 58 GTGAC ^GT[ACGT]AC 133 GTCAC MluNI 512 TGGCCA TGG^CCA 584 TGGCCA MscI 512 TGGCCA TGG^CCA 584 TGGCCA NarI 265 GGCGCC GG^CGCC 469 GGCGCC NciI 561 CCCGG CC^[GC]GG 562 CCGGG PflMI 79 CCACCCGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 160 CCATATGCTGG RsaI 48 GTAC GT^AC 334 GTAC SfaNI 89 GCATC GCATC(5/9) 323 GCATC SfoI 265 GGCGCC GGC^GCC 469 GGCGCC StyI 224 CCAAGG C^C[AT][AT]GG 581 CCATGG Tsp45I 58 GTGAC ^GT[GC]AC 133 GTCAC Van91I 79 CCACCCGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 160 CCATATGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AciI AluI ApaI AvaI AvaII BanII BmyI BsaJI BseRI BsiYI BslI BsmFI BsoBI Bsp1286I BssKI BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeII MboI MflI MnlI MspI MvaI MwoI NdeII NlaIII NlaIV PpuMI Sau3AI Sau96I ScrFI TspRI XhoII