(Circular) (Six-base) MAPSORT of: pFN21AE3067.seq Check: 8440 from: 1 to: 5371 pFN21AE3067 5371 bp With 182 enzymes: SgfI * March 28, 2012 12:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3181 278 Size: 53 83 186 936 1645 2468 Fragments arranged by size: 2468 1645 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2714 1937 Size: 777 4594 Acc65I G'GTAC_C Cuts at: 2692 2692 Size: 5371 AclI AA'CG_TT Cuts at: 1563 3498 3871 1563 Size: 1935 373 3063 Fragments arranged by size: 3063 1935 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2550 3434 4818 5086 1632 Size: 918 884 1384 268 1917 Fragments arranged by size: 1917 1384 918 884 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5174 AflIII A'CryG_T Cuts at: 1203 2335 4270 5247 1203 Size: 1132 1935 977 1327 Fragments arranged by size: 1935 1327 1132 977 AgeI A'CCGG_T Cuts at: 4202 4202 Size: 5371 AhdI GACnn_n'nnGTC Cuts at: 4100 4100 Size: 5371 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5371 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5371 AlwNI CAG_nnn'CTG Cuts at: 3114 4686 3114 Size: 1572 3799 ApaI G_GGCC'C Cuts at: 2153 2449 2487 2515 2557 2567 2153 Size: 296 38 28 42 10 4957 Fragments arranged by size: 4957 296 42 38 28 10 ApaLI G'TGCA_C Cuts at: 3428 4584 3428 Size: 1156 4215 ApoI r'AATT_y Cuts at: 1493 2680 2906 3058 5167 1493 Size: 1187 226 152 2109 1697 Fragments arranged by size: 2109 1697 1187 226 152 AseI AT'TA_AT Cuts at: 160 1032 3925 160 Size: 872 2893 1606 Fragments arranged by size: 2893 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5371 AvaI C'yCGr_G Cuts at: 1870 1943 2378 2442 2566 2696 1870 Size: 73 435 64 124 130 4545 Fragments arranged by size: 4545 435 130 124 73 64 BamHI G'GATC_C Cuts at: 2701 3068 2701 Size: 367 5004 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2270 2474 2692 4147 618 Size: 325 217 627 483 204 218 1455 1842 Fragments arranged by size: 1842 1455 627 483 325 218 217 204 BanII G_rGCy'C Cuts at: 1391 2153 2449 2487 2515 2557 2567 2690 1391 Size: 762 296 38 28 42 10 123 4072 Fragments arranged by size: 4072 762 296 123 42 38 28 10 BbeI G_GCGC'C Cuts at: 2274 2478 2274 Size: 204 5167 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4400 BbvCI CC'TCA_GC Cuts at: 2577 2577 Size: 5371 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2417 2451 3562 3596 1903 Size: 34 480 34 1111 34 3678 Fragments arranged by size: 3678 1111 480 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3263 4473 3263 Size: 1210 4161 BclI T'GATC_A Cuts at: 1548 1548 Size: 5371 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2510 3982 136 Size: 107 122 71 631 605 838 1472 1525 Fragments arranged by size: 1525 1472 838 631 605 122 107 71 BglII A'GATC_T Cuts at: 2250 2283 5366 2250 Size: 33 3083 2255 Fragments arranged by size: 3083 2255 33 BlpI GC'TnA_GC Cuts at: 2319 2788 2319 Size: 469 4902 Bme1580I G_kGCm'C Cuts at: 1790 2153 2449 2487 2515 2557 2567 3432 4588 Size: 363 296 38 28 42 10 865 1156 Cuts at: 4588 1790 Size: 2573 Fragments arranged by size: 2573 1156 865 363 296 42 38 28 10 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5371 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3102 4060 442 Size: 416 53 83 399 24 1685 958 1753 Fragments arranged by size: 1753 1685 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5371 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2117 2193 4031 1239 Size: 878 76 1838 2579 Fragments arranged by size: 2579 1838 878 76 Bpu10I CC'TnA_GC Cuts at: 2577 2577 Size: 5371 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2285 3496 4361 4659 2285 Size: 1211 865 298 2997 Fragments arranged by size: 2997 1211 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4034 882 Size: 617 2535 2219 Fragments arranged by size: 2535 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2338 3162 493 Size: 711 1134 824 2702 Fragments arranged by size: 2702 1134 824 711 BsaBI GATnn'nnATC Cuts at: 5365 5365 Size: 5371 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2271 2475 3178 3560 Size: 53 83 186 936 738 204 703 382 Cuts at: 3560 4195 275 Size: 635 1451 Fragments arranged by size: 1451 936 738 703 635 382 204 186 83 53 BsaWI w'CCGG_w Cuts at: 3804 4202 4476 4623 5034 3804 Size: 398 274 147 411 4141 Fragments arranged by size: 4141 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5146 Fragments arranged by size: 5146 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2129 2411 2444 1186 Size: 943 282 33 4113 Fragments arranged by size: 4113 943 282 33 BseYI C'CCAG_C Cuts at: 2200 2323 2486 4574 5070 2200 Size: 123 163 2088 496 2501 Fragments arranged by size: 2501 2088 496 163 123 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2370 1163 Size: 573 634 4164 Fragments arranged by size: 4164 634 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2509 2821 3582 4610 1540 Size: 275 186 508 312 761 1028 2301 Fragments arranged by size: 2301 1028 761 508 312 275 186 BsiHKAI G_wGCw'C Cuts at: 2301 3432 3517 4588 2301 Size: 1131 85 1071 3084 Fragments arranged by size: 3084 1131 1071 85 BsmI GAATG_Cn' Cuts at: 2882 2975 2882 Size: 93 5278 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2153 2301 2449 2487 2515 2557 2567 Size: 399 363 148 148 38 28 42 10 Cuts at: 2567 2690 3432 3517 4588 1391 Size: 123 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 363 148 148 123 85 42 38 28 10 BspEI T'CCGG_A Cuts at: 5034 5034 Size: 5371 BspHI T'CATG_A Cuts at: 3260 4990 3260 Size: 1730 3641 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2726 844 Size: 1882 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3258 1394 Size: 429 1435 3507 Fragments arranged by size: 3507 1435 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3866 4040 65 Size: 1183 2618 174 1396 Fragments arranged by size: 2618 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2477 2504 4015 4202 1700 Size: 110 667 27 1511 187 2869 Fragments arranged by size: 2869 1511 667 187 110 27 BsrGI T'GTAC_A Cuts at: 96 2052 96 Size: 1956 3415 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5371 BssSI C'ACGA_G Cuts at: 3431 4443 3431 Size: 1012 4359 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5371 BstBI TT'CG_AA Cuts at: 4175 4175 Size: 5371 BstEII G'GTnAC_C Cuts at: 1184 2137 1184 Size: 953 4418 BstYI r'GATC_y Cuts at: 1972 2250 2283 2463 2701 3068 3453 3470 4922 Size: 278 33 180 238 367 385 17 1452 Cuts at: 4922 5008 5020 5366 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 278 238 180 86 33 17 12 Bsu36I CC'TnA_GG Cuts at: 2154 2154 Size: 5371 BtgI C'CryG_G Cuts at: 513 1065 2467 2586 513 Size: 552 1402 119 3298 Fragments arranged by size: 3298 1402 552 119 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4632 Fragments arranged by size: 4632 683 56 BtsI GCAGTG_nn' Cuts at: 2887 3681 3701 2887 Size: 794 20 4557 Fragments arranged by size: 4557 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5371 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5336 DraI TTT'AAA Cuts at: 2675 2675 Size: 5371 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2135 4378 809 Size: 507 819 2243 1802 Fragments arranged by size: 2243 1802 819 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2517 2589 2628 Size: 54 1425 50 275 18 687 72 39 Cuts at: 2628 2818 3707 5336 8 Size: 190 889 1629 43 Fragments arranged by size: 1629 1425 889 687 275 190 72 54 50 43 39 18 EagI C'GGCC_G Cuts at: 1537 1812 2818 1537 Size: 275 1006 4090 Fragments arranged by size: 4090 1006 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3301 1353 Size: 1948 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2033 3958 4332 4478 387 Size: 1064 138 444 1925 374 146 1280 Fragments arranged by size: 1925 1280 1064 444 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2117 2193 2550 3434 4031 4818 5086 Size: 393 485 76 357 884 597 787 268 Cuts at: 5086 1239 Size: 1524 Fragments arranged by size: 1524 884 787 597 485 393 357 268 76 EcoO109I rG'GnC_Cy Cuts at: 2119 2149 2150 2160 2512 2553 2554 2563 2582 Size: 30 1 10 352 41 1 9 19 Cuts at: 2582 2119 Size: 4908 Fragments arranged by size: 4908 352 41 30 19 10 9 1 1 EcoRI G'AATT_C Cuts at: 2680 3058 2680 Size: 378 4993 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2254 2254 Size: 5371 FspI TGC'GCA Cuts at: 3877 3877 Size: 5371 HaeII r_GCGC'y Cuts at: 1678 2274 2478 2660 4518 1678 Size: 596 204 182 1858 2531 Fragments arranged by size: 2531 1858 596 204 182 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4012 4044 4086 4118 1373 Size: 32 135 32 2440 32 42 32 2626 Fragments arranged by size: 2626 2440 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2293 2715 2961 669 Size: 988 281 355 422 246 3079 Fragments arranged by size: 3079 988 422 355 281 246 HindIII A'AGCT_T Cuts at: 748 2532 748 Size: 1784 3587 HpaI GTT'AAC Cuts at: 2961 2961 Size: 5371 KasI G'GCGC_C Cuts at: 2270 2474 2270 Size: 204 5167 KpnI G_GTAC'C Cuts at: 2696 2696 Size: 5371 MfeI C'AATT_G Cuts at: 2970 2970 Size: 5371 MluI A'CGCG_T Cuts at: 5247 5247 Size: 5371 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2037 2356 4485 4669 1305 Size: 732 319 2129 184 2007 Fragments arranged by size: 2129 2007 732 319 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2519 2591 10 Size: 54 1425 343 687 72 2790 Fragments arranged by size: 2790 1425 687 343 72 54 MslI CAynn'nnrTG Cuts at: 518 1166 3329 3688 3847 518 Size: 648 2163 359 159 2042 Fragments arranged by size: 2163 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2490 2787 3464 4612 4857 1606 Size: 884 297 677 1148 245 2120 Fragments arranged by size: 2120 1148 884 677 297 245 NaeI GCC'GGC Cuts at: 2479 2479 Size: 5371 NarI GG'CG_CC Cuts at: 2271 2475 2271 Size: 204 5167 NcoI C'CATG_G Cuts at: 513 1065 2586 513 Size: 552 1521 3298 Fragments arranged by size: 3298 1521 552 NdeI CA'TA_TG Cuts at: 387 2167 387 Size: 1780 3591 NgoMIV G'CCGG_C Cuts at: 2477 2477 Size: 5371 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5371 NotI GC'GGCC_GC Cuts at: 2818 2818 Size: 5371 NspI r_CATG'y Cuts at: 1132 1229 2603 2729 4274 1132 Size: 97 1374 126 1545 2229 Fragments arranged by size: 2229 1545 1374 126 97 PasI CC'CwG_GG Cuts at: 1348 2549 2558 1348 Size: 1201 9 4161 Fragments arranged by size: 4161 1201 9 PciI A'CATG_T Cuts at: 4270 4270 Size: 5371 PflMI CCAn_nnn'nTGG Cuts at: 1108 2090 2171 1108 Size: 982 81 4308 Fragments arranged by size: 4308 982 81 PmeI GTTT'AAAC Cuts at: 2675 2675 Size: 5371 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3440 3472 4953 4985 3440 Size: 32 1481 32 3826 Fragments arranged by size: 3826 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2119 2160 2582 2119 Size: 41 422 4908 Fragments arranged by size: 4908 422 41 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5371 PsiI TTA'TAA Cuts at: 2941 2941 Size: 5371 PspOMI G'GGCC_C Cuts at: 2149 2445 2483 2511 2553 2563 2149 Size: 296 38 28 42 10 4957 Fragments arranged by size: 4957 296 42 38 28 10 PstI C_TGCA'G Cuts at: 830 2723 830 Size: 1893 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5371 SalI G'TCGA_C Cuts at: 1936 2713 1936 Size: 777 4594 SanDI GG'GwC_CC Cuts at: 2160 2582 2160 Size: 422 4949 SbfI CC_TGCA'GG Cuts at: 2723 2723 Size: 5371 ScaI AGT'ACT Cuts at: 3619 5331 3619 Size: 1712 3659 SfcI C'TryA_G Cuts at: 826 1045 2719 3854 4535 4726 826 Size: 219 1674 1135 681 191 1471 Fragments arranged by size: 1674 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2272 2476 2272 Size: 204 5167 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5371 SgrAI Cr'CCGG_yG Cuts at: 1700 2477 1700 Size: 777 4594 SmaI CCC'GGG Cuts at: 1872 2568 2698 1872 Size: 696 130 4545 Fragments arranged by size: 4545 696 130 SmlI C'TyrA_G Cuts at: 820 1017 1943 2300 3475 4376 4638 820 Size: 197 926 357 1175 901 262 1553 Fragments arranged by size: 1553 1175 926 901 357 262 197 SnaBI TAC'GTA Cuts at: 493 3162 493 Size: 2669 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5371 SphI G_CATG'C Cuts at: 1132 2603 2729 1132 Size: 1471 126 3774 Fragments arranged by size: 3774 1471 126 SspI AAT'ATT Cuts at: 5 52 3295 5 Size: 47 3243 2081 Fragments arranged by size: 3243 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5371 StyI C'CwwG_G Cuts at: 513 1065 2229 2586 2810 513 Size: 552 1164 357 224 3074 Fragments arranged by size: 3074 1164 552 357 224 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2242 3398 3415 3568 3753 4198 325 Size: 1255 662 1156 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1156 662 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2052 3617 5329 Size: 274 80 33 51 1443 75 1565 1712 Cuts at: 5329 96 Size: 138 Fragments arranged by size: 1712 1565 1443 274 138 80 75 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5331 5363 5331 Size: 32 5339 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5371 XbaI T'CTAG_A Cuts at: 2707 2707 Size: 5371 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5371 XmaI C'CCGG_G Cuts at: 1870 2566 2696 1870 Size: 696 130 4545 Fragments arranged by size: 4545 696 130 XmnI GAAnn'nnTTC Cuts at: 2210 3500 2210 Size: 1290 4081 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3179 276 Size: 53 83 186 936 1645 2468 Fragments arranged by size: 2468 1645 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SrfI SwaI XcmI