(Linear) (Six-base) MAPSORT of: KIEE3067.seq Check: 3122 from: 1 to: 666 KIEE3067.orf With 182 enzymes: SgfI * March 22, 2012 16:09 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 545 666 Size: 545 121 AflIII A'CryG_T Cuts at: 0 330 666 Size: 330 336 ApaI G_GGCC'C Cuts at: 0 148 444 482 510 552 562 666 Size: 148 296 38 28 42 10 104 Fragments arranged by size: 296 148 104 42 38 28 10 AvaI C'yCGr_G Cuts at: 0 373 437 561 666 Size: 373 64 124 105 Fragments arranged by size: 373 124 105 64 BanI G'GyrC_C Cuts at: 0 265 469 666 Size: 265 204 197 Fragments arranged by size: 265 204 197 BanII G_rGCy'C Cuts at: 0 148 444 482 510 552 562 666 Size: 148 296 38 28 42 10 104 Fragments arranged by size: 296 148 104 42 38 28 10 BbeI G_GCGC'C Cuts at: 0 269 473 666 Size: 269 204 193 Fragments arranged by size: 269 204 193 BbvCI CC'TCA_GC Cuts at: 0 572 666 Size: 572 94 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 412 446 666 Size: 412 34 220 Fragments arranged by size: 412 220 34 BglI GCCn_nnn'nGGC Cuts at: 0 505 666 Size: 505 161 BglII A'GATC_T Cuts at: 0 245 278 666 Size: 245 33 388 Fragments arranged by size: 388 245 33 BlpI GC'TnA_GC Cuts at: 0 314 666 Size: 314 352 Bme1580I G_kGCm'C Cuts at: 0 148 444 482 510 552 562 666 Size: 148 296 38 28 42 10 104 Fragments arranged by size: 296 148 104 42 38 28 10 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 112 188 666 Size: 112 76 478 Fragments arranged by size: 478 112 76 Bpu10I CC'TnA_GC Cuts at: 0 572 666 Size: 572 94 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 280 666 Size: 280 386 BsaAI yAC'GTr Cuts at: 0 333 666 Size: 333 333 BsaHI Gr'CG_yC Cuts at: 0 266 470 666 Size: 266 204 196 Fragments arranged by size: 266 204 196 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 124 406 439 666 Size: 124 282 33 227 Fragments arranged by size: 282 227 124 33 BseYI C'CCAG_C Cuts at: 0 195 318 481 666 Size: 195 123 163 185 Fragments arranged by size: 195 185 163 123 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 365 666 Size: 365 301 BsiEI CG_ry'CG Cuts at: 0 504 666 Size: 504 162 BsiHKAI G_wGCw'C Cuts at: 0 296 666 Size: 296 370 Bsp1286I G_dGCh'C Cuts at: 0 148 296 444 482 510 552 562 666 Size: 148 148 148 38 28 42 10 104 Fragments arranged by size: 148 148 148 104 42 38 28 10 BsrFI r'CCGG_y Cuts at: 0 472 499 666 Size: 472 27 167 Fragments arranged by size: 472 167 27 BsrGI T'GTAC_A Cuts at: 0 47 666 Size: 47 619 BstEII G'GTnAC_C Cuts at: 0 132 666 Size: 132 534 BstYI r'GATC_y Cuts at: 0 245 278 458 666 Size: 245 33 180 208 Fragments arranged by size: 245 208 180 33 Bsu36I CC'TnA_GG Cuts at: 0 149 666 Size: 149 517 BtgI C'CryG_G Cuts at: 0 462 581 666 Size: 462 119 85 Fragments arranged by size: 462 119 85 DrdI GACnn_nn'nnGTC Cuts at: 0 130 666 Size: 130 536 EaeI y'GGCC_r Cuts at: 0 512 584 623 666 Size: 512 72 39 43 Fragments arranged by size: 512 72 43 39 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 28 666 Size: 28 638 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 112 188 545 666 Size: 112 76 357 121 Fragments arranged by size: 357 121 112 76 EcoO109I rG'GnC_Cy Cuts at: 0 114 144 145 155 507 548 549 558 Size: 114 30 1 10 352 41 1 9 Cuts at: 558 577 666 Size: 19 89 Fragments arranged by size: 352 114 89 41 30 19 10 9 1 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 249 666 Size: 249 417 HaeII r_GCGC'y Cuts at: 0 269 473 655 666 Size: 269 204 182 11 Fragments arranged by size: 269 204 182 11 HincII GTy'rAC Cuts at: 0 288 666 Size: 288 378 HindIII A'AGCT_T Cuts at: 0 527 666 Size: 527 139 KasI G'GCGC_C Cuts at: 0 265 469 666 Size: 265 204 197 Fragments arranged by size: 265 204 197 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 32 351 666 Size: 32 319 315 Fragments arranged by size: 319 315 32 MscI TGG'CCA Cuts at: 0 514 586 666 Size: 514 72 80 Fragments arranged by size: 514 80 72 MspA1I CmG'CkG Cuts at: 0 485 666 Size: 485 181 NaeI GCC'GGC Cuts at: 0 474 666 Size: 474 192 NarI GG'CG_CC Cuts at: 0 266 470 666 Size: 266 204 196 Fragments arranged by size: 266 204 196 NcoI C'CATG_G Cuts at: 0 581 666 Size: 581 85 NdeI CA'TA_TG Cuts at: 0 162 666 Size: 162 504 NgoMIV G'CCGG_C Cuts at: 0 472 666 Size: 472 194 NspI r_CATG'y Cuts at: 0 598 666 Size: 598 68 PasI CC'CwG_GG Cuts at: 0 544 553 666 Size: 544 9 113 Fragments arranged by size: 544 113 9 PflMI CCAn_nnn'nTGG Cuts at: 0 85 166 666 Size: 85 81 500 Fragments arranged by size: 500 85 81 PpuMI rG'GwC_Cy Cuts at: 0 114 155 577 666 Size: 114 41 422 89 Fragments arranged by size: 422 114 89 41 PspOMI G'GGCC_C Cuts at: 0 144 440 478 506 548 558 666 Size: 144 296 38 28 42 10 108 Fragments arranged by size: 296 144 108 42 38 28 10 SanDI GG'GwC_CC Cuts at: 0 155 577 666 Size: 155 422 89 Fragments arranged by size: 422 155 89 SfoI GGC'GCC Cuts at: 0 267 471 666 Size: 267 204 195 Fragments arranged by size: 267 204 195 SgrAI Cr'CCGG_yG Cuts at: 0 472 666 Size: 472 194 SmaI CCC'GGG Cuts at: 0 563 666 Size: 563 103 SmlI C'TyrA_G Cuts at: 0 295 666 Size: 295 371 SphI G_CATG'C Cuts at: 0 598 666 Size: 598 68 StyI C'CwwG_G Cuts at: 0 224 581 666 Size: 224 357 85 Fragments arranged by size: 357 224 85 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 237 666 Size: 237 429 TatI w'GTAC_w Cuts at: 0 47 666 Size: 47 619 XmaI C'CCGG_G Cuts at: 0 561 666 Size: 561 105 XmnI GAAnn'nnTTC Cuts at: 0 205 666 Size: 205 461 Enzymes that do cut: AcuI AflIII ApaI AvaI BanI BanII BbeI BbvCI BcgI BglI BglII BlpI Bme1580I BpmI Bpu10I BpuEI BsaAI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BsrFI BsrGI BstEII BstYI Bsu36I BtgI DrdI EaeI EciI Eco57MI EcoO109I FalI HaeII HincII HindIII KasI MmeI MscI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PasI PflMI PpuMI PspOMI SanDI SfoI SgrAI SmaI SmlI SphI StyI TaqII TatI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI BciVI BclI BfrBI BmgBI BmrI BmtI BplI BsaI BsaBI BsaWI BsaXI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BssHII BssSI BstAPI BstBI BstXI BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII EagI EarI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI Hin4I HpaI KpnI MfeI MluI MslI NheI NotI NruI NsiI PacI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI SnaBI SpeI SrfI SspI StuI SwaI TstI Tth111I XbaI XcmI XhoI ZraI