################################## # NO CUT ENZYMES ( 120 enzymes) # ################################## AatII AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BbsI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaI BseAI BseRI BsiWI BsmAI BsmBI BsmI BspDI BspEI BspHI BspLU11I BsrBI BsrDI BssHII Bst1107I BstAPI BstBI BstZ17I CelII ClaI CpoI DraI DraIII DrdI Eam1105I EarI Eco47III EcoNI EcoRI EcoRV EcoT22I FbaI FspI HincII HindII HindIII HpaI Ksp632I MaeI MamI MfeI MluI MroI MunI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SalI SapI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TfiI Tth111I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 23 enzymes) # ################################### Acc65I 429 GGTACC G^GTACC AocI 295 CCTGAGG CC^T[ACGT]AGG Asp718I 429 GGTACC G^GTACC BamHI 929 GGATCC G^GATCC BbrPI 499 CACGTG CAC^GTG BsmFI 84 GGGAC GGGAC(10/14) BspMI 94 ACCTGC ACCTGC(4/8) BsrI 54 ACTGG ACTGG(1/-1) BssSI 934 CACGAG CACGAG(-5/-1) BstF5I 144 CATCC GGATG(2/0) BstXI 785 CCACCCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 295 CCTGAGG CC^T[ACGT]AGG Eco81I 295 CCTGAGG CC^T[ACGT]AGG FokI 144 CATCC GGATG(9/13) HinfI 571 GACTC G^A[ACGT]TC KpnI 429 GGTACC GGTAC^C MseI 484 TTAA T^TAA PleI 571 GACTC GAGTC(4/5) PmaCI 499 CACGTG CAC^GTG PmlI 499 CACGTG CAC^GTG SacI 415 GAGCTC GAGCT^C SmlI 478 CTCAAG C^T[CT][GA]AG Tru9I 484 TTAA T^TAA ################################### # TWO CUT ENZYMES ( 40 enzymes) # ################################### ApaI 800 GGGCCC GGGCC^C 970 GGGCCC AspHI 229 GTGCTC G[AT]GC[AT]^C 415 GAGCTC AvaII 912 GGACC G^G[AT]CC 1014 GGTCC BalI 740 TGGCCA TGG^CCA 782 TGGCCA BbeI 1045 GGCGCC GGCGC^C 1056 GGCGCC BbvCI 543 CCTCAGC CCTCAGC(-5/-2) 471 GCTGAGG BcgI 98 GCAAGACTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 908 GCAAGGACCTCG BpmI 793 CTGGAG CTGGAG(16/14) 1081 CTGGAG BsaAI 499 CACGTG [CT]AC^GT[GA] 1105 TACGTG BsaWI 282 ACCGGA [AT]^CCGG[AT] 601 ACCGGA BsgI 275 GTGCAG GTGCAG(16/14) 1164 GTGCAG BsiHKAI 229 GTGCTC G[AT]GC[AT]^C 415 GAGCTC BstEII 321 GGTCACC G^GT[ACGT]ACC 733 GGTGACC BstPI 321 GGTCACC G^GT[ACGT]ACC 733 GGTGACC BstYI 155 AGATCC [GA]^GATC[CT] 929 GGATCC EcoO65I 321 GGTCACC G^GT[ACGT]ACC 733 GGTGACC FseI 70 GGCCGGCC GGCCGG^CC 74 GGCCGGCC HgaI 63 GACGC GACGC(5/10) 465 GCGTC KasI 1045 GGCGCC G^GCGCC 1056 GGCGCC KspI 80 CCGCGG CCGC^GG 147 CCGCGG MaeII 500 ACGT A^CGT 1106 ACGT MboII 870 GAAGA GAAGA(8/7) 401 TCTTC MflI 155 AGATCC [GA]^GATC[CT] 929 GGATCC MluNI 740 TGGCCA TGG^CCA 782 TGGCCA MscI 740 TGGCCA TGG^CCA 782 TGGCCA NaeI 71 GCCGGC GCC^GGC 75 GCCGGC NarI 1045 GGCGCC GG^CGCC 1056 GGCGCC NgoMIV 71 GCCGGC G^CCGGC 75 GCCGGC PpuMI 911 AGGACCT [GA]G^G[AT]CC[CT] 1013 AGGTCCT PvuII 889 CAGCTG CAG^CTG 1132 CAGCTG SacII 80 CCGCGG CCGC^GG 147 CCGCGG SfaNI 134 GCATC GCATC(5/9) 807 GCATC SfoI 1045 GGCGCC GGC^GCC 1056 GGCGCC SmaI 452 CCCGGG CCC^GGG 1041 CCCGGG TaqI 107 TCGA T^CGA 137 TCGA Tsp509I 529 AATT ^AATT 639 AATT TthHB8I 107 TCGA T^CGA 137 TCGA XhoII 155 AGATCC [GA]^GATC[CT] 929 GGATCC XmaCI 452 CCCGGG C^CCGGG 1041 CCCGGG XmaI 452 CCCGGG C^CCGGG 1041 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 75 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AvaI BanI BanII BbvI BcnI BglI BmyI BsaHI BsaJI BsiEI BsiYI BslI BsoBI Bsp1286I Bsp1407I BsrFI BsrGI BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EclXI Eco52I EcoO109I EcoRII EcoT14I FauI Fnu4HI HaeII HaeIII HapII HhaI Hin1I HinP1I HpaII HphI ItaI MaeIII MboI MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SspBI StyI TseI Tsp45I TspRI