(Circular) (Six-base) MAPSORT of: pFN21AE1869.seq Check: 5660 from: 1 to: 5875 pFN21AE1869 5875 bp With 182 enzymes: SgfI * December 12, 2011 16:58 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2108 2108 Size: 5875 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3685 278 Size: 53 83 186 936 2149 2468 Fragments arranged by size: 2468 2149 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3218 1937 Size: 1281 4594 Acc65I G'GTAC_C Cuts at: 2434 3196 2434 Size: 762 5113 AclI AA'CG_TT Cuts at: 1563 4002 4375 1563 Size: 2439 373 3063 Fragments arranged by size: 3063 2439 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2845 3938 5322 5590 1632 Size: 1213 1093 1384 268 1917 Fragments arranged by size: 1917 1384 1213 1093 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5678 AflIII A'CryG_T Cuts at: 1203 4774 5751 1203 Size: 3571 977 1327 Fragments arranged by size: 3571 1327 977 AgeI A'CCGG_T Cuts at: 4706 4706 Size: 5875 AhdI GACnn_n'nnGTC Cuts at: 4604 4604 Size: 5875 AleI CACnn'nnGTG Cuts at: 1166 2333 1166 Size: 1167 4708 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2576 1184 Size: 1392 4483 AlwNI CAG_nnn'CTG Cuts at: 2164 2815 3085 3618 5190 2164 Size: 651 270 533 1572 2849 Fragments arranged by size: 2849 1572 651 533 270 ApaI G_GGCC'C Cuts at: 2809 2979 2809 Size: 170 5705 ApaLI G'TGCA_C Cuts at: 3932 5088 3932 Size: 1156 4719 ApoI r'AATT_y Cuts at: 1493 3184 3410 3562 5671 1493 Size: 1691 226 152 2109 1697 Fragments arranged by size: 2109 1697 1691 226 152 AseI AT'TA_AT Cuts at: 160 1032 4429 160 Size: 872 3397 1606 Fragments arranged by size: 3397 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5875 AvaI C'yCGr_G Cuts at: 1870 1943 2191 2225 2294 2457 2972 3046 3200 Size: 73 248 34 69 163 515 74 154 Cuts at: 3200 1870 Size: 4545 Fragments arranged by size: 4545 515 248 163 154 74 73 69 34 BamHI G'GATC_C Cuts at: 2934 3205 3572 2934 Size: 271 367 5237 Fragments arranged by size: 5237 367 271 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2206 2434 2566 2829 3050 Size: 325 217 627 419 228 132 263 221 Cuts at: 3050 3061 3196 4651 618 Size: 11 135 1455 1842 Fragments arranged by size: 1842 1455 627 419 325 263 228 221 217 135 132 11 BanII G_rGCy'C Cuts at: 1391 2295 2424 2809 2842 2946 2979 3194 1391 Size: 904 129 385 33 104 33 215 4072 Fragments arranged by size: 4072 904 385 215 129 104 33 33 BbeI G_GCGC'C Cuts at: 3054 3065 3054 Size: 11 5864 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4904 BbvCI CC'TCA_GC Cuts at: 2477 2549 2477 Size: 72 5803 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2092 2126 2902 2936 4066 Size: 34 50 34 71 34 776 34 1130 Cuts at: 4066 4100 1903 Size: 34 3678 Fragments arranged by size: 3678 1130 776 71 50 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3767 4977 3767 Size: 1210 4665 BclI T'GATC_A Cuts at: 1548 1548 Size: 5875 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2017 2077 3059 Size: 107 122 71 631 605 345 60 982 Cuts at: 3059 4486 136 Size: 1427 1525 Fragments arranged by size: 1525 1427 982 631 605 345 122 107 71 60 BglII A'GATC_T Cuts at: 5870 5870 Size: 5875 BlpI GC'TnA_GC Cuts at: 3292 3292 Size: 5875 Bme1580I G_kGCm'C Cuts at: 1790 2809 2979 3936 5092 1790 Size: 1019 170 957 1156 2573 Fragments arranged by size: 2573 1156 1019 957 170 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5875 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3606 4564 442 Size: 416 53 83 399 24 2189 958 1753 Fragments arranged by size: 2189 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5875 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2819 3107 4535 1239 Size: 1580 288 1428 2579 Fragments arranged by size: 2579 1580 1428 288 Bpu10I CC'TnA_GC Cuts at: 2477 2549 2660 2477 Size: 72 111 5692 Fragments arranged by size: 5692 111 72 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2468 4000 4865 5163 2468 Size: 1532 865 298 3180 Fragments arranged by size: 3180 1532 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4538 882 Size: 617 3039 2219 Fragments arranged by size: 3039 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2506 3112 3666 493 Size: 711 1302 606 554 2702 Fragments arranged by size: 2702 1302 711 606 554 BsaBI GATnn'nnATC Cuts at: 5869 5869 Size: 5875 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2069 3051 3062 3682 Size: 53 83 186 936 536 982 11 620 Cuts at: 3682 4064 4699 275 Size: 382 635 1451 Fragments arranged by size: 1451 982 936 635 620 536 382 186 83 53 11 BsaWI w'CCGG_w Cuts at: 2287 2606 4308 4706 4980 5127 5538 2287 Size: 319 1702 398 274 147 411 2624 Fragments arranged by size: 2624 1702 411 398 319 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3125 3155 1178 Size: 30 165 30 1722 30 3898 Fragments arranged by size: 3898 1722 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5875 BseYI C'CCAG_C Cuts at: 2570 2808 5078 5574 2570 Size: 238 2270 496 2871 Fragments arranged by size: 2871 2270 496 238 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2301 3190 1163 Size: 573 565 889 3848 Fragments arranged by size: 3848 889 573 565 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2081 2085 2263 2731 3325 4086 Size: 275 186 80 4 178 468 594 761 Cuts at: 4086 5114 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 761 594 468 275 186 178 80 4 BsiHKAI G_wGCw'C Cuts at: 2238 2424 3936 4021 5092 2238 Size: 186 1512 85 1071 3021 Fragments arranged by size: 3021 1512 1071 186 85 BsmI GAATG_Cn' Cuts at: 3386 3479 3386 Size: 93 5782 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2238 2295 2424 2809 2842 2946 2979 Size: 399 448 57 129 385 33 104 33 Cuts at: 2979 3194 3936 4021 5092 1391 Size: 215 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 448 399 385 215 129 104 85 57 33 33 BspEI T'CCGG_A Cuts at: 5538 5538 Size: 5875 BspHI T'CATG_A Cuts at: 3764 5494 3764 Size: 1730 4145 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2108 3230 844 Size: 1264 1122 3489 Fragments arranged by size: 3489 1264 1122 BsrBI CCG'CTC Cuts at: 1394 1823 3762 1394 Size: 429 1939 3507 Fragments arranged by size: 3507 1939 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4370 4544 65 Size: 1183 3122 174 1396 Fragments arranged by size: 3122 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2076 2080 2556 2726 2766 4519 4706 Size: 110 266 4 476 170 40 1753 187 Cuts at: 4706 1700 Size: 2869 Fragments arranged by size: 2869 1753 476 266 187 170 110 40 4 BsrGI T'GTAC_A Cuts at: 96 2244 2898 3030 96 Size: 2148 654 132 2941 Fragments arranged by size: 2941 2148 654 132 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5875 BssSI C'ACGA_G Cuts at: 2939 3935 4947 2939 Size: 996 1012 3867 Fragments arranged by size: 3867 1012 996 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5875 BstBI TT'CG_AA Cuts at: 4679 4679 Size: 5875 BstEII G'GTnAC_C Cuts at: 1184 2326 2738 1184 Size: 1142 412 4321 Fragments arranged by size: 4321 1142 412 BstXI CCAn_nnnn'nTGG Cuts at: 2797 2797 Size: 5875 BstYI r'GATC_y Cuts at: 1972 2160 2934 3205 3572 3957 3974 5426 5512 Size: 188 774 271 367 385 17 1452 86 Cuts at: 5512 5524 5870 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 774 385 367 346 271 188 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2301 2301 Size: 5875 BtgI C'CryG_G Cuts at: 513 1065 2085 2152 2632 2868 513 Size: 552 1020 67 480 236 3520 Fragments arranged by size: 3520 1020 552 480 236 67 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5136 Fragments arranged by size: 5136 683 56 BtsI GCAGTG_nn' Cuts at: 2627 2668 2696 3391 4185 4205 2627 Size: 41 28 695 794 20 4297 Fragments arranged by size: 4297 794 695 41 28 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5875 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5840 DraI TTT'AAA Cuts at: 3179 3179 Size: 5875 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4882 809 Size: 507 3566 1802 Fragments arranged by size: 3566 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2074 2078 2082 Size: 54 1425 50 275 18 244 4 4 Cuts at: 2082 2155 2268 2728 2745 2787 3322 4211 5840 Size: 73 113 460 17 42 535 889 1629 Cuts at: 5840 8 Size: 43 Fragments arranged by size: 1629 1425 889 535 460 275 244 113 73 54 50 43 42 18 17 4 4 EagI C'GGCC_G Cuts at: 1537 1812 2078 2082 2728 3322 1537 Size: 275 266 4 646 594 4090 Fragments arranged by size: 4090 646 594 275 266 4 EarI CTCTTCn'nnn_ Cuts at: 1353 3805 1353 Size: 2452 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4462 4836 4982 387 Size: 1064 138 2873 374 146 1280 Fragments arranged by size: 2873 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2422 2422 Size: 5875 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2819 2845 3107 3938 4535 5322 5590 Size: 393 1187 26 262 831 597 787 268 Cuts at: 5590 1239 Size: 1524 Fragments arranged by size: 1524 1187 831 787 597 393 268 262 26 EcoO109I rG'GnC_Cy Cuts at: 2805 2917 2976 3019 2805 Size: 112 59 43 5661 Fragments arranged by size: 5661 112 59 43 EcoRI G'AATT_C Cuts at: 3184 3562 3184 Size: 378 5497 FseI GG_CCGG'CC Cuts at: 2080 2084 2080 Size: 4 5871 FspI TGC'GCA Cuts at: 4381 4381 Size: 5875 HaeII r_GCGC'y Cuts at: 1678 2667 3054 3065 3086 5022 1678 Size: 989 387 11 21 1936 2531 Fragments arranged by size: 2531 1936 989 387 21 11 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2576 2608 3125 3157 4516 Size: 32 135 32 1004 32 517 32 1359 Cuts at: 4516 4548 4590 4622 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1359 1004 517 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3219 3465 669 Size: 988 281 1281 246 3079 Fragments arranged by size: 3079 1281 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5875 HpaI GTT'AAC Cuts at: 3465 3465 Size: 5875 KasI G'GCGC_C Cuts at: 3050 3061 3050 Size: 11 5864 KpnI G_GTAC'C Cuts at: 2438 3200 2438 Size: 762 5113 MfeI C'AATT_G Cuts at: 3474 3474 Size: 5875 MluI A'CGCG_T Cuts at: 5751 5751 Size: 5875 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3168 4989 5173 1305 Size: 1863 1821 184 2007 Fragments arranged by size: 2007 1863 1821 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2747 2789 10 Size: 54 1425 343 915 42 3096 Fragments arranged by size: 3096 1425 915 343 54 42 MslI CAynn'nnrTG Cuts at: 518 1166 2333 2399 3152 3833 4192 4351 518 Size: 648 1167 66 753 681 359 159 2042 Fragments arranged by size: 2042 1167 753 681 648 359 159 66 MspA1I CmG'CkG Cuts at: 1606 2047 2087 2154 2221 2797 2896 3094 3139 Size: 441 40 67 67 576 99 198 45 Cuts at: 3139 3291 3968 5116 5361 1606 Size: 152 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 576 441 245 198 152 99 67 67 45 40 NaeI GCC'GGC Cuts at: 2078 2082 2078 Size: 4 5871 NarI GG'CG_CC Cuts at: 3051 3062 3051 Size: 11 5864 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5323 NdeI CA'TA_TG Cuts at: 387 387 Size: 5875 NgoMIV G'CCGG_C Cuts at: 2076 2080 2076 Size: 4 5871 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5875 NotI GC'GGCC_GC Cuts at: 3322 3322 Size: 5875 NspI r_CATG'y Cuts at: 1132 1229 3233 4778 1132 Size: 97 2004 1545 2229 Fragments arranged by size: 2229 2004 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5875 PciI A'CATG_T Cuts at: 4774 4774 Size: 5875 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5875 PmeI GTTT'AAAC Cuts at: 3179 3179 Size: 5875 PmlI CAC'GTG Cuts at: 2506 2506 Size: 5875 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3944 3976 5457 5489 3944 Size: 32 1481 32 4330 Fragments arranged by size: 4330 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2917 3019 2917 Size: 102 5773 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5875 PsiI TTA'TAA Cuts at: 3445 3445 Size: 5875 PspOMI G'GGCC_C Cuts at: 2805 2975 2805 Size: 170 5705 PstI C_TGCA'G Cuts at: 830 3227 830 Size: 2397 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5875 PvuII CAG'CTG Cuts at: 2896 3139 2896 Size: 243 5632 SacI G_AGCT'C Cuts at: 2424 2424 Size: 5875 SacII CC_GC'GG Cuts at: 2088 2155 2088 Size: 67 5808 SalI G'TCGA_C Cuts at: 1936 3217 1936 Size: 1281 4594 SbfI CC_TGCA'GG Cuts at: 3227 3227 Size: 5875 ScaI AGT'ACT Cuts at: 4123 5835 4123 Size: 1712 4163 SfcI C'TryA_G Cuts at: 826 1045 3223 4358 5039 5230 826 Size: 219 2178 1135 681 191 1471 Fragments arranged by size: 2178 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 3052 3063 3052 Size: 11 5864 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5875 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5875 SmaI CCC'GGG Cuts at: 1872 2459 3048 3202 1872 Size: 587 589 154 4545 Fragments arranged by size: 4545 589 587 154 SmlI C'TyrA_G Cuts at: 820 1017 1943 2483 3979 4880 5142 820 Size: 197 926 540 1496 901 262 1553 Fragments arranged by size: 1553 1496 926 901 540 262 197 SnaBI TAC'GTA Cuts at: 493 3666 493 Size: 3173 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5875 SphI G_CATG'C Cuts at: 1132 3233 1132 Size: 2101 3774 SspI AAT'ATT Cuts at: 5 52 3799 5 Size: 47 3747 2081 Fragments arranged by size: 3747 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5875 StyI C'CwwG_G Cuts at: 513 1065 2319 2946 3126 3314 513 Size: 552 1254 627 180 188 3074 Fragments arranged by size: 3074 1254 627 552 188 180 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2584 3902 3919 4072 4257 4702 325 Size: 1255 1004 1318 17 153 185 445 1498 Fragments arranged by size: 1498 1318 1255 1004 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2244 2384 2898 Size: 274 80 33 51 1443 267 140 514 Cuts at: 2898 3009 3030 3105 4121 5833 96 Size: 111 21 75 1016 1712 138 Fragments arranged by size: 1712 1443 1016 514 274 267 140 138 111 80 75 51 33 21 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5835 5867 5835 Size: 32 5843 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5875 XbaI T'CTAG_A Cuts at: 3211 3211 Size: 5875 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5875 XmaI C'CCGG_G Cuts at: 1870 2457 3046 3200 1870 Size: 587 589 154 4545 Fragments arranged by size: 4545 589 587 154 XmnI GAAnn'nnTTC Cuts at: 4004 4004 Size: 5875 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3683 276 Size: 53 83 186 936 2149 2468 Fragments arranged by size: 2468 2149 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI FspAI NruI NsiI PacI PfoI PspXI PsrI RsrII SanDI SapI SexAI SfiI SrfI SwaI XcmI