(Circular) (Six-base) MAPSORT of: pFN21AE4595.seq Check: 8697 from: 1 to: 8428 pFN21AE4595 8428 bp With 182 enzymes: SgfI * March 28, 2012 14:19 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2298 3949 2298 Size: 1651 6777 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6238 278 Size: 53 83 186 936 4702 2468 Fragments arranged by size: 4702 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5011 5771 1937 Size: 3074 760 4594 Fragments arranged by size: 4594 3074 760 Acc65I G'GTAC_C Cuts at: 2250 5749 2250 Size: 3499 4929 AclI AA'CG_TT Cuts at: 1563 6555 6928 1563 Size: 4992 373 3063 Fragments arranged by size: 4992 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2079 2861 3247 5151 5160 5422 5643 6491 Size: 447 782 386 1904 9 262 221 848 Cuts at: 6491 7875 8143 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1904 1384 848 782 447 386 268 262 221 9 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8231 AflIII A'CryG_T Cuts at: 1203 3251 4728 4760 7327 8304 1203 Size: 2048 1477 32 2567 977 1327 Fragments arranged by size: 2567 2048 1477 1327 977 32 AgeI A'CCGG_T Cuts at: 7259 7259 Size: 8428 AhdI GACnn_n'nnGTC Cuts at: 2732 7157 2732 Size: 4425 4003 AleI CACnn'nnGTG Cuts at: 1166 2833 4253 4593 4692 1166 Size: 1667 1420 340 99 4902 Fragments arranged by size: 4902 1667 1420 340 99 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2204 3020 1184 Size: 1020 816 6592 Fragments arranged by size: 6592 1020 816 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2558 2590 2558 Size: 32 8396 AlwNI CAG_nnn'CTG Cuts at: 2510 2647 2839 3148 3334 3964 4363 5037 6171 Size: 137 192 309 186 630 399 674 1134 Cuts at: 6171 7743 2510 Size: 1572 3195 Fragments arranged by size: 3195 1572 1134 674 630 399 309 192 186 137 ApaI G_GGCC'C Cuts at: 2113 2222 2364 2113 Size: 109 142 8177 Fragments arranged by size: 8177 142 109 ApaLI G'TGCA_C Cuts at: 4211 6485 7641 4211 Size: 2274 1156 4998 Fragments arranged by size: 4998 2274 1156 ApoI r'AATT_y Cuts at: 1493 2282 5737 5963 6115 8224 1493 Size: 789 3455 226 152 2109 1697 Fragments arranged by size: 3455 2109 1697 789 226 152 AseI AT'TA_AT Cuts at: 160 1032 6982 160 Size: 872 5950 1606 Fragments arranged by size: 5950 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8428 AvaI C'yCGr_G Cuts at: 1870 1943 2097 2245 2363 2805 3381 3534 3679 Size: 73 154 148 118 442 576 153 145 Cuts at: 3679 4031 4312 4599 5176 5481 5753 1870 Size: 352 281 287 577 305 272 4545 Fragments arranged by size: 4545 577 576 442 352 305 287 281 272 154 153 148 145 118 73 BamHI G'GATC_C Cuts at: 2232 5638 5758 6125 2232 Size: 3406 120 367 4535 Fragments arranged by size: 4535 3406 367 120 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2250 2438 4181 4401 4471 Size: 325 217 627 463 188 1743 220 70 Cuts at: 4471 5749 7204 618 Size: 1278 1455 1842 Fragments arranged by size: 1842 1743 1455 1278 627 463 325 220 217 188 70 BanII G_rGCy'C Cuts at: 1391 2113 2222 2364 2370 2576 2630 3426 3796 Size: 722 109 142 6 206 54 796 370 Cuts at: 3796 3894 3911 4114 5387 5747 1391 Size: 98 17 203 1273 360 4072 Fragments arranged by size: 4072 1273 796 722 370 360 206 203 142 109 98 54 17 6 BbeI G_GCGC'C Cuts at: 4405 4475 4405 Size: 70 8358 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2937 5148 5309 5631 928 Size: 971 1038 2211 161 322 3725 Fragments arranged by size: 3725 2211 1038 971 322 161 BbvCI CC'TCA_GC Cuts at: 2312 5286 5711 2312 Size: 2974 425 5029 Fragments arranged by size: 5029 2974 425 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6619 6653 1903 Size: 34 4682 34 3678 Fragments arranged by size: 4682 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6320 7530 6320 Size: 1210 7218 BclI T'GATC_A Cuts at: 1548 1548 Size: 8428 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2343 4034 4427 Size: 107 122 71 631 605 671 1691 393 Cuts at: 4427 7039 136 Size: 2612 1525 Fragments arranged by size: 2612 1691 1525 671 631 605 393 122 107 71 BglII A'GATC_T Cuts at: 8423 8423 Size: 8428 BlpI GC'TnA_GC Cuts at: 2948 3602 5845 2948 Size: 654 2243 5531 Fragments arranged by size: 5531 2243 654 Bme1580I G_kGCm'C Cuts at: 1790 2113 2222 2364 2766 2876 4184 4215 4237 Size: 323 109 142 402 110 1308 31 22 Cuts at: 4237 4600 5526 6489 7645 1790 Size: 363 926 963 1156 2573 Fragments arranged by size: 2573 1308 1156 963 926 402 363 323 142 110 109 31 22 BmgBI CAC'GTC Cuts at: 1318 4210 1318 Size: 2892 5536 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4336 4346 6159 Size: 416 53 83 399 24 2919 10 1813 Cuts at: 6159 7117 442 Size: 958 1753 Fragments arranged by size: 2919 1813 1753 958 416 399 83 53 24 10 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8428 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2161 2405 2963 5311 2161 Size: 244 558 2348 5278 Fragments arranged by size: 5278 2348 558 244 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2696 2706 3785 4223 4461 4581 4901 4935 Size: 1457 10 1079 438 238 120 320 34 Cuts at: 4935 5151 5583 7088 1239 Size: 216 432 1505 2579 Fragments arranged by size: 2579 1505 1457 1079 438 432 320 238 216 120 34 10 Bpu10I CC'TnA_GC Cuts at: 2024 2312 2821 5286 5711 2024 Size: 288 509 2465 425 4741 Fragments arranged by size: 4741 2465 509 425 288 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 6553 7418 7716 6553 Size: 865 298 7265 Fragments arranged by size: 7265 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3278 5120 7091 882 Size: 617 1779 1842 1971 2219 Fragments arranged by size: 2219 1971 1842 1779 617 BsaAI yAC'GTr Cuts at: 493 1204 2473 2767 3585 6219 493 Size: 711 1269 294 818 2634 2702 Fragments arranged by size: 2702 2634 1269 818 711 294 BsaBI GATnn'nnATC Cuts at: 3049 4299 8422 3049 Size: 1250 4123 3055 Fragments arranged by size: 4123 3055 1250 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2660 3687 4402 4472 Size: 53 83 186 936 1127 1027 715 70 Cuts at: 4472 5278 6235 6617 7252 275 Size: 806 957 382 635 1451 Fragments arranged by size: 1451 1127 1027 957 936 806 715 635 382 186 83 70 53 BsaWI w'CCGG_w Cuts at: 6861 7259 7533 7680 8091 6861 Size: 398 274 147 411 7198 Fragments arranged by size: 7198 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2318 2348 2558 2588 2780 Size: 30 165 30 915 30 210 30 192 Cuts at: 2780 2810 4476 4506 5342 5372 5456 5486 1178 Size: 30 1666 30 836 30 84 30 4120 Fragments arranged by size: 4120 1666 915 836 210 192 165 84 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3173 4364 5216 5363 5480 1186 Size: 1987 1191 852 147 117 4134 Fragments arranged by size: 4134 1987 1191 852 147 117 BseYI C'CCAG_C Cuts at: 2459 2577 2668 3130 3374 5091 5445 7631 8127 Size: 118 91 462 244 1717 354 2186 496 Cuts at: 8127 2459 Size: 2760 Fragments arranged by size: 2760 2186 1717 496 462 354 244 118 91 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2276 3647 4145 4270 5612 1163 Size: 573 540 1371 498 125 1342 3979 Fragments arranged by size: 3979 1371 1342 573 540 498 125 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2347 5878 6639 7667 1540 Size: 275 186 346 3531 761 1028 2301 Fragments arranged by size: 3531 2301 1028 761 346 275 186 BsiHKAI G_wGCw'C Cuts at: 2370 2630 2883 2994 3527 3894 3911 4215 4530 Size: 260 253 111 533 367 17 304 315 Cuts at: 4530 4680 5387 6489 6574 7645 2370 Size: 150 707 1102 85 1071 3153 Fragments arranged by size: 3153 1102 1071 707 533 367 315 304 260 253 150 111 85 17 BsmI GAATG_Cn' Cuts at: 5939 6032 5939 Size: 93 8335 BsmBI CGTCTCn'nnnn_ Cuts at: 3678 3678 Size: 8428 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2113 2222 2364 2370 2576 2630 2766 Size: 399 323 109 142 6 206 54 136 Cuts at: 2766 2876 2883 2994 3426 3527 3796 3894 3911 Size: 110 7 111 432 101 269 98 17 Cuts at: 3911 4114 4184 4215 4237 4530 4600 4680 5387 Size: 203 70 31 22 293 70 80 707 Cuts at: 5387 5526 5747 6489 6574 7645 1391 Size: 139 221 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 707 432 399 323 293 269 221 206 203 142 139 136 111 110 109 101 98 85 80 70 70 54 31 22 17 7 6 BspEI T'CCGG_A Cuts at: 8091 8091 Size: 8428 BspHI T'CATG_A Cuts at: 6317 8047 6317 Size: 1730 6698 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2298 2998 3878 3949 5783 844 Size: 1454 700 880 71 1834 3489 Fragments arranged by size: 3489 1834 1454 880 700 71 BsrBI CCG'CTC Cuts at: 1394 1823 2349 5371 5424 6315 1394 Size: 429 526 3022 53 891 3507 Fragments arranged by size: 3507 3022 891 526 429 53 BsrDI GCAATG_nn' Cuts at: 65 1248 2198 4719 6923 7097 65 Size: 1183 950 2521 2204 174 1396 Fragments arranged by size: 2521 2204 1396 1183 950 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2854 3727 7072 7259 1700 Size: 110 1044 873 3345 187 2869 Fragments arranged by size: 3345 2869 1044 873 187 110 BsrGI T'GTAC_A Cuts at: 96 4763 96 Size: 4667 3761 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8428 BssSI C'ACGA_G Cuts at: 2369 6488 7500 2369 Size: 4119 1012 3297 Fragments arranged by size: 4119 3297 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3148 794 Size: 2354 6074 BstBI TT'CG_AA Cuts at: 7232 7232 Size: 8428 BstEII G'GTnAC_C Cuts at: 1184 2446 3010 3970 1184 Size: 1262 564 960 5642 Fragments arranged by size: 5642 1262 960 564 BstXI CCAn_nnnn'nTGG Cuts at: 2011 3619 4694 5129 2011 Size: 1608 1075 435 5310 Fragments arranged by size: 5310 1608 1075 435 BstYI r'GATC_y Cuts at: 1972 2232 3044 4651 4660 5638 5758 6125 6510 Size: 260 812 1607 9 978 120 367 385 Cuts at: 6510 6527 7979 8065 8077 8423 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1607 1452 978 812 385 367 346 260 120 86 17 12 9 Bsu36I CC'TnA_GG Cuts at: 5429 5429 Size: 8428 BtgI C'CryG_G Cuts at: 513 1065 2004 2442 2935 3107 3153 3975 4054 Size: 552 939 438 493 172 46 822 79 Cuts at: 4054 4185 4872 5388 513 Size: 131 687 516 3553 Fragments arranged by size: 3553 939 822 687 552 516 493 438 172 131 79 46 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2331 4076 487 Size: 683 56 1105 1745 4839 Fragments arranged by size: 4839 1745 1105 683 56 BtsI GCAGTG_nn' Cuts at: 2188 2828 2840 2908 3002 3671 4162 4588 5944 Size: 640 12 68 94 669 491 426 1356 Cuts at: 5944 6738 6758 2188 Size: 794 20 3858 Fragments arranged by size: 3858 1356 794 669 640 491 426 94 68 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8428 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8393 DraI TTT'AAA Cuts at: 5732 5732 Size: 8428 DraIII CAC_nnn'GTG Cuts at: 2868 4873 4966 2868 Size: 2005 93 6330 Fragments arranged by size: 6330 2005 93 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5277 7435 809 Size: 507 3961 2158 1802 Fragments arranged by size: 3961 2158 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2344 2744 2848 Size: 54 1425 50 275 18 514 400 104 Cuts at: 2848 3657 3774 3856 5046 5664 5875 6764 8393 Size: 809 117 82 1190 618 211 889 1629 Cuts at: 8393 8 Size: 43 Fragments arranged by size: 1629 1425 1190 889 809 618 514 400 275 211 117 104 82 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2344 5875 1537 Size: 275 532 3531 4090 Fragments arranged by size: 4090 3531 532 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2594 4760 5316 6358 1353 Size: 1241 2166 556 1042 3423 Fragments arranged by size: 3423 2166 1241 1042 556 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3714 3799 7015 7389 7535 387 Size: 1064 138 2125 85 3216 374 146 1280 Fragments arranged by size: 3216 2125 1280 1064 374 146 138 85 EcoICRI GAG'CTC Cuts at: 2368 2628 3892 3909 5385 2368 Size: 260 1264 17 1476 5411 Fragments arranged by size: 5411 1476 1264 260 17 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2079 2696 2706 2861 3247 3785 4223 Size: 393 447 617 10 155 386 538 438 Cuts at: 4223 4461 4581 4901 4935 5151 5160 5422 5583 Size: 238 120 320 34 216 9 262 161 Cuts at: 5583 5643 6491 7088 7875 8143 1239 Size: 60 848 597 787 268 1524 Fragments arranged by size: 1524 848 787 617 597 538 447 438 393 386 320 268 262 238 216 161 155 120 60 34 10 9 EcoNI CCTnn'n_nnAGG Cuts at: 2274 2756 4544 4836 2274 Size: 482 1788 292 5866 Fragments arranged by size: 5866 1788 482 292 EcoO109I rG'GnC_Cy Cuts at: 2110 2142 2219 2266 2360 2680 2726 2982 3347 Size: 32 77 47 94 320 46 256 365 Cuts at: 3347 3378 4243 4642 4906 4939 5088 5171 5254 Size: 31 865 399 264 33 149 83 83 Cuts at: 5254 5573 5600 5608 2110 Size: 319 27 8 4930 Fragments arranged by size: 4930 865 399 365 320 319 264 256 149 94 83 83 77 47 46 33 32 31 27 8 EcoRI G'AATT_C Cuts at: 2282 5737 6115 2282 Size: 3455 378 4595 Fragments arranged by size: 4595 3455 378 EcoRV GAT'ATC Cuts at: 4383 4383 Size: 8428 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3304 4912 3304 Size: 1608 6820 FspI TGC'GCA Cuts at: 6934 6934 Size: 8428 HaeII r_GCGC'y Cuts at: 1678 3888 4405 4443 4475 7575 1678 Size: 2210 517 38 32 3100 2531 Fragments arranged by size: 3100 2531 2210 517 38 32 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2357 2389 2718 2750 3331 Size: 32 135 32 785 32 329 32 581 Cuts at: 3331 3363 4367 4399 4476 4508 4820 4852 5019 Size: 32 1004 32 77 32 312 32 167 Cuts at: 5019 5051 5300 5332 5456 5488 5704 5736 7069 Size: 32 249 32 124 32 216 32 1333 Cuts at: 7069 7101 7143 7175 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1333 1004 785 581 329 312 249 216 167 135 124 77 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2479 3733 5772 6018 669 Size: 988 281 541 1254 2039 246 3079 Fragments arranged by size: 3079 2039 1254 988 541 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8428 HpaI GTT'AAC Cuts at: 6018 6018 Size: 8428 KasI G'GCGC_C Cuts at: 4401 4471 4401 Size: 70 8358 KpnI G_GTAC'C Cuts at: 2254 5753 2254 Size: 3499 4929 MfeI C'AATT_G Cuts at: 6027 6027 Size: 8428 MluI A'CGCG_T Cuts at: 8304 8304 Size: 8428 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3228 3543 5298 7542 7726 1305 Size: 1923 315 1755 2244 184 2007 Fragments arranged by size: 2244 2007 1923 1755 315 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2746 2850 3659 3858 5048 Size: 54 1425 343 914 104 809 199 1190 Cuts at: 5048 10 Size: 3390 Fragments arranged by size: 3390 1425 1190 914 809 343 199 104 54 MslI CAynn'nnrTG Cuts at: 518 1166 2833 3197 4253 4593 4692 5529 6386 Size: 648 1667 364 1056 340 99 837 857 Cuts at: 6386 6745 6904 518 Size: 359 159 2042 Fragments arranged by size: 2042 1667 1056 857 837 648 364 359 340 159 99 MspA1I CmG'CkG Cuts at: 1606 2952 3109 3145 3619 3977 4056 4076 4107 Size: 1346 157 36 474 358 79 20 31 Cuts at: 4107 4159 4187 4559 5149 5266 5449 5844 6521 Size: 52 28 372 590 117 183 395 677 Cuts at: 6521 7669 7914 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1346 1148 677 590 474 395 372 358 245 183 157 117 79 52 36 31 28 20 NarI GG'CG_CC Cuts at: 4402 4472 4402 Size: 70 8358 NcoI C'CATG_G Cuts at: 513 1065 2004 2442 2935 3153 513 Size: 552 939 438 493 218 5788 Fragments arranged by size: 5788 939 552 493 438 218 NdeI CA'TA_TG Cuts at: 387 387 Size: 8428 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8428 NotI GC'GGCC_GC Cuts at: 5875 5875 Size: 8428 NspI r_CATG'y Cuts at: 1132 1229 3255 3705 4321 4764 5786 7331 1132 Size: 97 2026 450 616 443 1022 1545 2229 Fragments arranged by size: 2229 2026 1545 1022 616 450 443 97 PasI CC'CwG_GG Cuts at: 1348 2011 4942 5519 5604 1348 Size: 663 2931 577 85 4172 Fragments arranged by size: 4172 2931 663 577 85 PciI A'CATG_T Cuts at: 3251 4760 7327 3251 Size: 1509 2567 4352 Fragments arranged by size: 4352 2567 1509 PflMI CCAn_nnn'nTGG Cuts at: 1108 2868 2893 4363 4966 1108 Size: 1760 25 1470 603 4570 Fragments arranged by size: 4570 1760 1470 603 25 PfoI T'CCnGG_A Cuts at: 2722 4239 2722 Size: 1517 6911 PmeI GTTT'AAAC Cuts at: 5732 5732 Size: 8428 PmlI CAC'GTG Cuts at: 2767 3585 2767 Size: 818 7610 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2558 2590 6497 6529 8010 8042 2558 Size: 32 3907 32 1481 32 2944 Fragments arranged by size: 3907 2944 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2266 2726 2982 3347 3378 4243 4642 5088 5171 Size: 460 256 365 31 865 399 446 83 Cuts at: 5171 5254 5573 5600 5608 2266 Size: 83 319 27 8 5086 Fragments arranged by size: 5086 865 460 446 399 365 319 256 83 83 31 27 8 PshAI GACnn'nnGTC Cuts at: 1613 3237 3704 1613 Size: 1624 467 6337 Fragments arranged by size: 6337 1624 467 PsiI TTA'TAA Cuts at: 5998 5998 Size: 8428 PspOMI G'GGCC_C Cuts at: 2109 2218 2360 2109 Size: 109 142 8177 Fragments arranged by size: 8177 142 109 PstI C_TGCA'G Cuts at: 830 2835 2980 3009 4558 5780 830 Size: 2005 145 29 1549 1222 3478 Fragments arranged by size: 3478 2005 1549 1222 145 29 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8428 PvuII CAG'CTG Cuts at: 2952 3145 3619 4159 4559 5149 5449 2952 Size: 193 474 540 400 590 300 5931 Fragments arranged by size: 5931 590 540 474 400 300 193 SacI G_AGCT'C Cuts at: 2370 2630 3894 3911 5387 2370 Size: 260 1264 17 1476 5411 Fragments arranged by size: 5411 1476 1264 260 17 SacII CC_GC'GG Cuts at: 3110 3978 4057 4188 3110 Size: 868 79 131 7350 Fragments arranged by size: 7350 868 131 79 SalI G'TCGA_C Cuts at: 1936 5770 1936 Size: 3834 4594 SanDI GG'GwC_CC Cuts at: 2266 2982 3347 3378 4642 5088 5573 5600 2266 Size: 716 365 31 1264 446 485 27 5094 Fragments arranged by size: 5094 1264 716 485 446 365 31 27 SbfI CC_TGCA'GG Cuts at: 5780 5780 Size: 8428 ScaI AGT'ACT Cuts at: 6676 8388 6676 Size: 1712 6716 SexAI A'CCwGG_T Cuts at: 5658 5658 Size: 8428 SfcI C'TryA_G Cuts at: 826 1045 2831 2976 3005 4554 5012 5776 6911 Size: 219 1786 145 29 1549 458 764 1135 Cuts at: 6911 7592 7783 826 Size: 681 191 1471 Fragments arranged by size: 1786 1549 1471 1135 764 681 458 219 191 145 29 SfoI GGC'GCC Cuts at: 4403 4473 4403 Size: 70 8358 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8428 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8428 SmaI CCC'GGG Cuts at: 1872 2099 3383 3681 4314 5755 1872 Size: 227 1284 298 633 1441 4545 Fragments arranged by size: 4545 1441 1284 633 298 227 SmlI C'TyrA_G Cuts at: 820 1017 1943 6532 7433 7695 820 Size: 197 926 4589 901 262 1553 Fragments arranged by size: 4589 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 6219 493 Size: 5726 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8428 SphI G_CATG'C Cuts at: 1132 5786 1132 Size: 4654 3774 SspI AAT'ATT Cuts at: 5 52 6352 5 Size: 47 6300 2081 Fragments arranged by size: 6300 2081 47 StuI AGG'CCT Cuts at: 1517 2064 2303 2693 2751 3260 4037 1517 Size: 547 239 390 58 509 777 5908 Fragments arranged by size: 5908 777 547 509 390 239 58 StyI C'CwwG_G Cuts at: 513 1065 2004 2442 2935 3153 3279 4780 5058 Size: 552 939 438 493 218 126 1501 278 Cuts at: 5058 5867 513 Size: 809 3074 Fragments arranged by size: 3074 1501 939 809 552 493 438 278 218 126 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2900 3476 5343 6455 6472 6625 6810 Size: 1255 1320 576 1867 1112 17 153 185 Cuts at: 6810 7255 325 Size: 445 1498 Fragments arranged by size: 1867 1498 1320 1255 1112 576 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4763 6674 8386 Size: 274 80 33 51 1443 2786 1911 1712 Cuts at: 8386 96 Size: 138 Fragments arranged by size: 2786 1911 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2318 2350 2780 2812 5342 5374 5709 5741 8388 Size: 32 430 32 2530 32 335 32 2647 Cuts at: 8388 8420 2318 Size: 32 2326 Fragments arranged by size: 2647 2530 2326 430 335 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3463 4280 5330 1316 Size: 2147 817 1050 4414 Fragments arranged by size: 4414 2147 1050 817 XbaI T'CTAG_A Cuts at: 5764 5764 Size: 8428 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3037 5056 5126 3037 Size: 2019 70 6339 Fragments arranged by size: 6339 2019 70 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8428 XmaI C'CCGG_G Cuts at: 1870 2097 3381 3679 4312 5753 1870 Size: 227 1284 298 633 1441 4545 Fragments arranged by size: 4545 1441 1284 633 298 227 XmnI GAAnn'nnTTC Cuts at: 2588 6557 2588 Size: 3969 4459 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6236 276 Size: 53 83 186 936 4702 2468 Fragments arranged by size: 4702 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BfrBI BsiWI BstZ17I FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SapI SfiI SrfI SwaI