(Circular) (Six-base) MAPSORT of: pFN21AB9620.seq Check: 2724 from: 1 to: 5746 pFN21AB9620 5746 bp With 182 enzymes: SgfI * March 28, 2012 14:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2164 2953 2164 Size: 789 4957 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3556 278 Size: 53 83 186 936 2020 2468 Fragments arranged by size: 2468 2020 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2583 3089 1937 Size: 646 506 4594 Fragments arranged by size: 4594 646 506 Acc65I G'GTAC_C Cuts at: 3067 3067 Size: 5746 AclI AA'CG_TT Cuts at: 1563 3873 4246 1563 Size: 2310 373 3063 Fragments arranged by size: 3063 2310 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2312 3809 5193 5461 1632 Size: 680 1497 1384 268 1917 Fragments arranged by size: 1917 1497 1384 680 268 AfeI AGC'GCT Cuts at: 2187 2187 Size: 5746 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5549 AflIII A'CryG_T Cuts at: 1203 4645 5622 1203 Size: 3442 977 1327 Fragments arranged by size: 3442 1327 977 AgeI A'CCGG_T Cuts at: 4577 4577 Size: 5746 AhdI GACnn_n'nnGTC Cuts at: 4475 4475 Size: 5746 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5746 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2846 1184 Size: 1662 4084 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2393 2425 2393 Size: 32 5714 AlwNI CAG_nnn'CTG Cuts at: 3009 3019 3489 5061 3009 Size: 10 470 1572 3694 Fragments arranged by size: 3694 1572 470 10 ApaI G_GGCC'C Cuts at: 2511 2511 Size: 5746 ApaLI G'TGCA_C Cuts at: 3803 4959 3803 Size: 1156 4590 ApoI r'AATT_y Cuts at: 1493 3055 3281 3433 5542 1493 Size: 1562 226 152 2109 1697 Fragments arranged by size: 2109 1697 1562 226 152 AseI AT'TA_AT Cuts at: 160 1032 4300 160 Size: 872 3268 1606 Fragments arranged by size: 3268 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5746 AvaI C'yCGr_G Cuts at: 1870 1943 2086 2102 2306 2312 2512 3071 1870 Size: 73 143 16 204 6 200 559 4545 Fragments arranged by size: 4545 559 204 200 143 73 16 6 BamHI G'GATC_C Cuts at: 3076 3443 3076 Size: 367 5379 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2054 2107 2234 2248 2612 Size: 325 217 627 267 53 127 14 364 Cuts at: 2612 2630 2740 3034 3067 4522 618 Size: 18 110 294 33 1455 1842 Fragments arranged by size: 1842 1455 627 364 325 294 267 217 127 110 53 33 18 14 BanII G_rGCy'C Cuts at: 1391 2313 2366 2511 2531 2771 3065 1391 Size: 922 53 145 20 240 294 4072 Fragments arranged by size: 4072 922 294 240 145 53 20 BbeI G_GCGC'C Cuts at: 2058 2111 2238 2616 2634 2058 Size: 53 127 378 18 5170 Fragments arranged by size: 5170 378 127 53 18 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2912 928 Size: 971 1013 3762 Fragments arranged by size: 3762 1013 971 BbvCI CC'TCA_GC Cuts at: 2990 2990 Size: 5746 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2824 2858 2935 2969 3937 3971 1903 Size: 34 887 34 77 34 968 34 3678 Fragments arranged by size: 3678 968 887 77 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3638 4848 3638 Size: 1210 4536 BclI T'GATC_A Cuts at: 1548 1548 Size: 5746 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2049 2558 4357 Size: 107 122 71 631 605 377 509 1799 Cuts at: 4357 136 Size: 1525 Fragments arranged by size: 1799 1525 631 605 509 377 122 107 71 BglII A'GATC_T Cuts at: 5741 5741 Size: 5746 BlpI GC'TnA_GC Cuts at: 2036 2159 3163 2036 Size: 123 1004 4619 Fragments arranged by size: 4619 1004 123 Bme1580I G_kGCm'C Cuts at: 1790 2511 2519 2667 2745 3039 3807 4963 1790 Size: 721 8 148 78 294 768 1156 2573 Fragments arranged by size: 2573 1156 768 721 294 148 78 8 BmgBI CAC'GTC Cuts at: 1318 2749 2998 1318 Size: 1431 249 4066 Fragments arranged by size: 4066 1431 249 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3477 4435 442 Size: 416 53 83 399 24 2060 958 1753 Fragments arranged by size: 2060 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5746 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2899 2899 Size: 5746 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2456 2894 4406 1239 Size: 1217 438 1512 2579 Fragments arranged by size: 2579 1512 1217 438 Bpu10I CC'TnA_GC Cuts at: 2594 2990 2594 Size: 396 5350 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2174 2705 2897 2960 3871 4736 5034 2174 Size: 531 192 63 911 865 298 2886 Fragments arranged by size: 2886 911 865 531 298 192 63 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4409 882 Size: 617 2910 2219 Fragments arranged by size: 2910 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3537 493 Size: 711 2333 2702 Fragments arranged by size: 2702 2333 711 BsaBI GATnn'nnATC Cuts at: 5740 5740 Size: 5746 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2055 2108 2235 2613 Size: 53 83 186 936 522 53 127 378 Cuts at: 2613 2631 2936 3553 3935 4570 275 Size: 18 305 617 382 635 1451 Fragments arranged by size: 1451 936 635 617 522 382 378 305 186 127 83 53 53 18 BsaWI w'CCGG_w Cuts at: 4179 4577 4851 4998 5409 4179 Size: 398 274 147 411 4516 Fragments arranged by size: 4516 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2393 2423 2921 2951 1178 Size: 30 165 30 990 30 498 30 3973 Fragments arranged by size: 3973 990 498 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2414 1186 Size: 1228 4518 BseYI C'CCAG_C Cuts at: 2240 3024 4949 5445 2240 Size: 784 1925 496 2541 Fragments arranged by size: 2541 1925 784 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5173 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2121 2127 2202 2478 2565 2643 Size: 275 186 120 6 75 276 87 78 Cuts at: 2643 3196 3957 4985 1540 Size: 553 761 1028 2301 Fragments arranged by size: 2301 1028 761 553 276 275 186 120 87 78 75 6 BsiHKAI G_wGCw'C Cuts at: 2313 2366 3807 3892 4963 2313 Size: 53 1441 85 1071 3096 Fragments arranged by size: 3096 1441 1071 85 53 BsiWI C'GTAC_G Cuts at: 3042 3042 Size: 5746 BsmI GAATG_Cn' Cuts at: 3257 3350 3257 Size: 93 5653 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2313 2366 2511 2519 2531 2667 2745 Size: 399 523 53 145 8 12 136 78 Cuts at: 2745 2771 3039 3065 3807 3892 4963 1391 Size: 26 268 26 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 523 399 268 145 136 85 78 53 26 26 12 8 BspEI T'CCGG_A Cuts at: 5409 5409 Size: 5746 BspHI T'CATG_A Cuts at: 3635 5365 3635 Size: 1730 4016 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2164 2953 3101 844 Size: 1320 789 148 3489 Fragments arranged by size: 3489 1320 789 148 BsrBI CCG'CTC Cuts at: 1394 1823 2040 3633 1394 Size: 429 217 1593 3507 Fragments arranged by size: 3507 1593 429 217 BsrDI GCAATG_nn' Cuts at: 65 1248 4241 4415 65 Size: 1183 2993 174 1396 Fragments arranged by size: 2993 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2126 4390 4577 1700 Size: 110 316 2264 187 2869 Fragments arranged by size: 2869 2264 316 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5746 BssHII G'CGCG_C Cuts at: 1925 2224 1925 Size: 299 5447 BssSI C'ACGA_G Cuts at: 3806 4818 3806 Size: 1012 4734 BstAPI GCAn_nnn'nTGC Cuts at: 794 3034 794 Size: 2240 3506 BstBI TT'CG_AA Cuts at: 4550 4550 Size: 5746 BstEII G'GTnAC_C Cuts at: 1184 2344 1184 Size: 1160 4586 BstXI CCAn_nnnn'nTGG Cuts at: 2341 2791 2341 Size: 450 5296 BstYI r'GATC_y Cuts at: 1972 3076 3443 3828 3845 5297 5383 5395 5741 Size: 1104 367 385 17 1452 86 12 346 Cuts at: 5741 1972 Size: 1977 Fragments arranged by size: 1977 1452 1104 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2121 2523 2940 513 Size: 552 939 117 402 417 3319 Fragments arranged by size: 3319 939 552 417 402 117 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2040 2827 487 Size: 683 56 814 787 3406 Fragments arranged by size: 3406 814 787 683 56 BtsI GCAGTG_nn' Cuts at: 3262 4056 4076 3262 Size: 794 20 4932 Fragments arranged by size: 4932 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5746 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5711 DraI TTT'AAA Cuts at: 3050 3050 Size: 5746 DraIII CAC_nnn'GTG Cuts at: 3034 3034 Size: 5746 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4753 809 Size: 507 3437 1802 Fragments arranged by size: 3437 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2118 2124 2199 Size: 54 1425 50 275 18 288 6 75 Cuts at: 2199 2391 2475 2520 2562 3193 4082 5711 8 Size: 192 84 45 42 631 889 1629 43 Fragments arranged by size: 1629 1425 889 631 288 275 192 84 75 54 50 45 43 42 18 6 EagI C'GGCC_G Cuts at: 1537 1812 2118 2124 2199 2475 2562 3193 1537 Size: 275 306 6 75 276 87 631 4090 Fragments arranged by size: 4090 631 306 276 275 87 75 6 EarI CTCTTCn'nnn_ Cuts at: 1353 2889 3676 1353 Size: 1536 787 3423 Fragments arranged by size: 3423 1536 787 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2831 4333 4707 4853 387 Size: 1064 138 1242 1502 374 146 1280 Fragments arranged by size: 1502 1280 1242 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2311 2364 2311 Size: 53 5693 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2312 2456 2894 3809 4406 5193 5461 Size: 393 680 144 438 915 597 787 268 Cuts at: 5461 1239 Size: 1524 Fragments arranged by size: 1524 915 787 680 597 438 393 268 144 EcoO109I rG'GnC_Cy Cuts at: 2431 2507 2508 2431 Size: 76 1 5669 Fragments arranged by size: 5669 76 1 EcoRI G'AATT_C Cuts at: 3055 3433 3055 Size: 378 5368 FspI TGC'GCA Cuts at: 2834 4252 2834 Size: 1418 4328 HaeII r_GCGC'y Cuts at: 1678 2036 2058 2111 2189 2238 2320 2579 2616 Size: 358 22 53 78 49 82 259 37 Cuts at: 2616 2634 4893 1678 Size: 18 2259 2531 Fragments arranged by size: 2531 2259 358 259 82 78 53 49 37 22 18 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2921 2953 4387 4419 4461 Size: 32 135 32 1349 32 1434 32 42 Cuts at: 4461 4493 1373 Size: 32 2626 Fragments arranged by size: 2626 1434 1349 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3001 3090 3336 669 Size: 988 281 1063 89 246 3079 Fragments arranged by size: 3079 1063 988 281 246 89 HindIII A'AGCT_T Cuts at: 748 748 Size: 5746 HpaI GTT'AAC Cuts at: 3336 3336 Size: 5746 KasI G'GCGC_C Cuts at: 2054 2107 2234 2612 2630 2054 Size: 53 127 378 18 5170 Fragments arranged by size: 5170 378 127 53 18 KpnI G_GTAC'C Cuts at: 3071 3071 Size: 5746 MfeI C'AATT_G Cuts at: 3345 3345 Size: 5746 MluI A'CGCG_T Cuts at: 5622 5622 Size: 5746 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2358 4860 5044 1305 Size: 1053 2502 184 2007 Fragments arranged by size: 2502 2007 1053 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3924 Fragments arranged by size: 3924 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2789 3704 4063 4222 518 Size: 648 1623 915 359 159 2042 Fragments arranged by size: 2042 1623 915 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2067 2123 2480 3006 3016 3162 3839 4987 Size: 461 56 357 526 10 146 677 1148 Cuts at: 4987 5232 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 526 461 357 245 146 56 10 NaeI GCC'GGC Cuts at: 2128 2128 Size: 5746 NarI GG'CG_CC Cuts at: 2055 2108 2235 2613 2631 2055 Size: 53 127 378 18 5170 Fragments arranged by size: 5170 378 127 53 18 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4255 Fragments arranged by size: 4255 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5746 NgoMIV G'CCGG_C Cuts at: 2126 2126 Size: 5746 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5746 NotI GC'GGCC_GC Cuts at: 2199 2475 2562 3193 2199 Size: 276 87 631 4752 Fragments arranged by size: 4752 631 276 87 NspI r_CATG'y Cuts at: 1132 1229 3104 4649 1132 Size: 97 1875 1545 2229 Fragments arranged by size: 2229 1875 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5746 PciI A'CATG_T Cuts at: 4645 4645 Size: 5746 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5746 PmeI GTTT'AAAC Cuts at: 3050 3050 Size: 5746 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2393 2425 3815 3847 5328 5360 2393 Size: 32 1390 32 1481 32 2779 Fragments arranged by size: 2779 1481 1390 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5746 PsiI TTA'TAA Cuts at: 3316 3316 Size: 5746 PspOMI G'GGCC_C Cuts at: 2507 2507 Size: 5746 PspXI vC'TCGA_Gb Cuts at: 2312 2312 Size: 5746 PstI C_TGCA'G Cuts at: 830 3098 830 Size: 2268 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5746 PvuII CAG'CTG Cuts at: 3016 3016 Size: 5746 SacI G_AGCT'C Cuts at: 2313 2366 2313 Size: 53 5693 SacII CC_GC'GG Cuts at: 2124 2124 Size: 5746 SalI G'TCGA_C Cuts at: 1936 3088 1936 Size: 1152 4594 SbfI CC_TGCA'GG Cuts at: 3098 3098 Size: 5746 ScaI AGT'ACT Cuts at: 3994 5706 3994 Size: 1712 4034 SexAI A'CCwGG_T Cuts at: 3030 3030 Size: 5746 SfcI C'TryA_G Cuts at: 826 1045 3094 4229 4910 5101 826 Size: 219 2049 1135 681 191 1471 Fragments arranged by size: 2049 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2056 2109 2236 2614 2632 2056 Size: 53 127 378 18 5170 Fragments arranged by size: 5170 378 127 53 18 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5746 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5746 SmaI CCC'GGG Cuts at: 1872 2088 2104 3073 1872 Size: 216 16 969 4545 Fragments arranged by size: 4545 969 216 16 SmlI C'TyrA_G Cuts at: 820 1017 1943 2189 2312 2720 2912 2975 3850 Size: 197 926 246 123 408 192 63 875 Cuts at: 3850 4751 5013 820 Size: 901 262 1553 Fragments arranged by size: 1553 926 901 875 408 262 246 197 192 123 63 SnaBI TAC'GTA Cuts at: 493 3537 493 Size: 3044 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5746 SphI G_CATG'C Cuts at: 1132 3104 1132 Size: 1972 3774 SspI AAT'ATT Cuts at: 5 52 3670 5 Size: 47 3618 2081 Fragments arranged by size: 3618 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5746 StyI C'CwwG_G Cuts at: 513 1065 2004 2382 3185 513 Size: 552 939 378 803 3074 Fragments arranged by size: 3074 939 803 552 378 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3773 3790 3943 4128 4573 325 Size: 1255 2193 17 153 185 445 1498 Fragments arranged by size: 2193 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3992 5704 96 Size: 274 80 33 51 1443 2015 1712 138 Fragments arranged by size: 2015 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5706 5738 5706 Size: 32 5714 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5746 XbaI T'CTAG_A Cuts at: 3082 3082 Size: 5746 XhoI C'TCGA_G Cuts at: 1943 2312 1943 Size: 369 5377 XmaI C'CCGG_G Cuts at: 1870 2086 2102 3071 1870 Size: 216 16 969 4545 Fragments arranged by size: 4545 969 216 16 XmnI GAAnn'nnTTC Cuts at: 3875 3875 Size: 5746 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3554 276 Size: 53 83 186 936 2020 2468 Fragments arranged by size: 2468 2020 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BfrBI BsmBI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PsrI RsrII SanDI SapI SfiI SrfI SwaI XcmI