################################## # NO CUT ENZYMES ( 156 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AflII AgeI AhdI AlwNI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BclI BcnI BfrI BglII BlnI BlpI BpmI Bpu1102I BsaBI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmFI BsoBI BspDI BspEI BspLU11I BspMI BsrBI BsrI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I CelII CfoI ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HhaI HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MflI MluI MroI MspA1I MunI MvnI NarI NciI NcoI NdeI NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI StuI SwaI TaqI Tth111I TthHB8I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AcyI 817 GACGCC G[GA]^CG[CT]C AflIII 724 ACGTGT A^C[GA][CT]GT AluI 893 AGCT AG^CT Alw44I 192 GTGCAC G^TGCAC AlwI 522 GATCC GGATC(4/5) AocI 879 CCTAAGG CC^T[ACGT]AGG ApaLI 192 GTGCAC G^TGCAC BanII 411 GAGCCC G[GA]GC[CT]^C BbvI 673 GCTGC GCAGC(8/12) BciVI 730 GTATCC GTATCC(6/5) BfaI 5 CTAG C^TAG BglI 820 GCCTTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 723 TACGTG [CT]AC^GT[GA] BsaHI 817 GACGCC G[GA]^CG[CT]C BsmAI 205 GTCTC GTCTC(1/5) BsmBI 204 CGTCTC CGTCTC(1/5) BsmI 900 GAATGC GAATGC(1/-1) Bsp1407I 809 TGTACA T^GTACA BspHI 407 TCATGA T^CATGA BsrDI 116 CATTGC GCAATG(2/0) BsrFI 704 GCCGGC [GA]^CCGG[CT] BsrGI 809 TGTACA T^GTACA BstAPI 66 GCACCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 879 CCTAAGG CC^T[ACGT]AGG Cfr10I 704 GCCGGC [GA]^CCGG[CT] Cfr13I 795 GGGCC G^G[ACGT]CC DraII 794 AGGGCCT [GA]G^G[ACGT]CC[CT] EarI 184 CTCTTC CTCTTC(1/4) Eco81I 879 CCTAAGG CC^T[ACGT]AGG EcoO109I 794 AGGGCCT [GA]G^G[ACGT]CC[CT] EcoT14I 791 CCAAGG C^C[AT][AT]GG Fnu4HI 673 GCTGC GC^[ACGT]GC HapII 705 CCGG C^CGG HgaI 817 GACGC GACGC(5/10) Hin1I 817 GACGCC G[GA]^CG[CT]C HpaII 705 CCGG C^CGG ItaI 673 GCTGC GC^[ACGT]GC Ksp632I 184 CTCTTC CTCTTC(1/4) MaeI 5 CTAG C^TAG MaeII 724 ACGT A^CGT MseI 35 TTAA T^TAA MspI 705 CCGG C^CGG NaeI 704 GCCGGC GCC^GGC NgoMIV 704 GCCGGC G^CCGGC NspI 242 GCATGC [GA]CATG^[CT] PleI 164 GACTC GAGTC(4/5) RcaI 407 TCATGA T^CATGA Sau96I 795 GGGCC G^G[ACGT]CC SphI 242 GCATGC GCATG^C SspBI 809 TGTACA T^GTACA StyI 791 CCAAGG C^C[AT][AT]GG Tru9I 35 TTAA T^TAA TseI 673 GCTGC G^C[AT]GC XcmI 275 CCATCACCTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AcsI 31 GAATTT [GA]^AATT[CT] 928 AAATTC ApoI 31 GAATTT [GA]^AATT[CT] 928 AAATTC AspHI 192 GTGCAC G[AT]GC[AT]^C 403 GTGCTC BbsI 661 GTCTTC GAAGAC(2/6) 844 GTCTTC BpuAI 661 GTCTTC GAAGAC(2/6) 844 GTCTTC BsaJI 446 CCTGGG C^C[ACGT][ACGT]GG 791 CCAAGG BsiHKAI 192 GTGCAC G[AT]GC[AT]^C 403 GTGCTC BssKI 348 CCTGG ^CC[ACGT]GG 446 CCTGG BstF5I 648 CATCC GGATG(2/0) 470 GGATG BstNI 348 CCTGG CC^[AT]GG 446 CCTGG EcoRII 348 CCTGG ^CC[AT]GG 446 CCTGG FokI 648 CATCC GGATG(9/13) 470 GGATG MvaI 348 CCTGG CC^[AT]GG 446 CCTGG PflMI 143 CCACAATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 429 CCATCTTGTGG ScrFI 348 CCTGG CC^[ACGT]GG 446 CCTGG SfaNI 603 GATGC GCATC(5/9) 20 GCATC SfcI 15 CTATAG C^T[GA][CT]AG 759 CTATAG SmlI 681 CTTGAG C^T[CT][GA]AG 784 CTCAAG TfiI 213 GATTC G^A[AT]TC 687 GATTC Tsp509I 32 AATT ^AATT 929 AATT Van91I 143 CCACAATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 429 CCATCTTGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 27 enzymes) # ######################################### AfaI BalI BmyI BsiYI BslI Bsp1286I Cac8I DdeI DpnII EaeI HaeIII HinfI HphI MaeIII MboI MboII MluNI MnlI MscI MslI MwoI NdeII NlaIII RsaI Sau3AI Tsp45I TspRI