################################## # NO CUT ENZYMES ( 154 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AhdI Alw44I AocI ApaI ApaLI AscI Asp718I AspEI AspI AviII AvrII BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaBI BsaHI BsaI BseAI BseRI BsiEI BsiWI BsmBI BsmI BspDI BspEI BspLU11I BsrBI BsrDI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstZ17I Bsu36I CelII ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoO109I EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HgaI Hin1I HincII HindII HpaI KasI KpnI Ksp632I KspI MaeI MamI MfeI MluI MroI MspA1I MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StyI SwaI TaqI TfiI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccI 205 GTAGAC GT^[AC][GT]AC AciI 620 GCGG CCGC(-3/-1) AgeI 362 ACCGGT A^CCGGT AlwNI 809 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 609 AGCGCT AGC^GCT AseI 541 ATTAAT AT^TAAT AsnI 541 ATTAAT AT^TAAT AspHI 115 GTGCTC G[AT]GC[AT]^C AvaI 118 CTCGGG C^[CT]CG[GA]G BamHI 638 GGATCC G^GATCC BmyI 115 GTGCTC G[AGT]GC[ACT]^C BsaAI 674 TACGTG [CT]AC^GT[GA] BsaJI 42 CCTGGG C^C[ACGT][ACGT]GG BsaWI 362 ACCGGT [AT]^CCGG[AT] BsgI 388 CTGCAC GTGCAG(16/14) BsiHKAI 115 GTGCTC G[AT]GC[AT]^C BsoBI 118 CTCGGG C^[CT]CG[GA]G Bsp1286I 115 GTGCTC G[AGT]GC[ACT]^C BspHI 449 TCATGA T^CATGA BspMI 125 ACCTGC ACCTGC(4/8) BsrFI 362 ACCGGT [GA]^CCGG[CT] BsrI 634 ACTGG ACTGG(1/-1) BstEII 840 GGTCACC G^GT[ACGT]ACC BstPI 840 GGTCACC G^GT[ACGT]ACC BstXI 98 CCATGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 638 GGATCC [GA]^GATC[CT] CfoI 610 GCGC GCG^C Cfr10I 362 ACCGGT [GA]^CCGG[CT] Eco47III 609 AGCGCT AGC^GCT EcoO65I 840 GGTCACC G^GT[ACGT]ACC EcoRI 462 GAATTC G^AATTC HaeII 609 AGCGCT [GA]GCGC^[CT] HapII 363 CCGG C^CGG HhaI 610 GCGC GCG^C HinP1I 610 GCGC G^CGC HindIII 477 AAGCTT A^AGCTT HinfI 154 GACTC G^A[ACGT]TC HpaII 363 CCGG C^CGG MaeII 675 ACGT A^CGT MflI 638 GGATCC [GA]^GATC[CT] MspI 363 CCGG C^CGG NspI 760 ACATGC [GA]CATG^[CT] PinAI 362 ACCGGT A^CCGGT PleI 154 GACTC GAGTC(4/5) PshBI 541 ATTAAT AT^TAAT PstI 815 CTGCAG CTGCA^G RcaI 449 TCATGA T^CATGA SexAI 104 ACCTGGT A^CC[AT]GGT StuI 71 AGGCCT AGG^CCT XhoII 638 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AluI 478 AGCT AG^CT 690 AGCT AvaII 552 GGTCC G^G[AT]CC 625 GGTCC BalI 140 TGGCCA TGG^CCA 371 TGGCCA BsmAI 220 GTCTC GTCTC(1/5) 742 GTCTC BsmFI 232 GTCCC GGGAC(10/14) 626 GTCCC Bsp1407I 505 TGTACA T^GTACA 830 TGTACA BsrGI 505 TGTACA T^GTACA 830 TGTACA Cac8I 69 GCAGGC GC[ACGT]^[ACGT]GC 283 GCAGGC Cfr13I 552 GGTCC G^G[ACGT]CC 625 GGTCC DpnII 447 GATC ^GATC 639 GATC EaeI 140 TGGCCA [CT]^GGCC[GA] 371 TGGCCA MboI 447 GATC ^GATC 639 GATC MluNI 140 TGGCCA TGG^CCA 371 TGGCCA MscI 140 TGGCCA TGG^CCA 371 TGGCCA NdeII 447 GATC ^GATC 639 GATC Sau3AI 447 GATC ^GATC 639 GATC Sau96I 552 GGTCC G^G[ACGT]CC 625 GGTCC SfcI 815 CTGCAG C^T[GA][CT]AG 867 CTACAG SspBI 505 TGTACA T^GTACA 830 TGTACA ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AcsI AfaI AlwI ApoI Asp700I BbvI BsiYI BslI BssKI BstF5I BstNI DdeI EcoRII Fnu4HI FokI HaeIII HphI ItaI MaeIII MboII MnlI MseI MslI MvaI NlaIII NlaIV RsaI ScrFI SfaNI Tru9I TseI Tsp45I Tsp509I TspRI XmnI