################################## # NO CUT ENZYMES ( 153 enzymes) # ################################## AatII Acc65I AccII AclI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BanI BanII BbeI BbrPI BbsI BbvCI BcgI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI BstAPI BstBI BstUI Bsu36I CelII CfoI Cfr10I Cfr13I CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoRV EcoT22I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HpaI KasI KpnI KspI MamI MfeI MluI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SapI Sau96I SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccIII 60 TCCGGA T^CCGGA AciI 722 GCGG CCGC(-3/-1) Asp700I 31 GAATTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BamHI 57 GGATCC G^GATCC BciVI 920 GGATAC GTATCC(6/5) BsaWI 60 TCCGGA [AT]^CCGG[AT] BseAI 60 TCCGGA T^CCGGA BsgI 472 CTGCAC GTGCAG(16/14) BsmAI 664 GTCTC GTCTC(1/5) BspDI 362 ATCGAT AT^CGAT BspEI 60 TCCGGA T^CCGGA BspLU11I 887 ACATGT A^CATGT BspMI 125 ACCTGC ACCTGC(4/8) Bst1107I 831 GTATAC GTA^TAC BstYI 57 GGATCC [GA]^GATC[CT] BstZ17I 831 GTATAC GTA^TAC Cac8I 558 GCTCGC GC[ACGT]^[ACGT]GC ClaI 362 ATCGAT AT^CGAT DsaI 836 CCGTGG C^C[GA][CT]GG EarI 79 CTCTTC CTCTTC(1/4) EcoT14I 200 CCTTGG C^C[AT][AT]GG FauI 722 GCGGG CCCGC(4/6) HapII 61 CCGG C^CGG HindIII 707 AAGCTT A^AGCTT HinfI 154 GACTC G^A[ACGT]TC HpaII 61 CCGG C^CGG Ksp632I 79 CTCTTC CTCTTC(1/4) MaeII 478 ACGT A^CGT MboII 80 TCTTC GAAGA(8/7) MflI 57 GGATCC [GA]^GATC[CT] MroI 60 TCCGGA T^CCGGA MseI 733 TTAA T^TAA MspI 61 CCGG C^CGG NspI 887 ACATGT [GA]CATG^[CT] PflMI 685 CCATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 154 GACTC GAGTC(4/5) PstI 67 CTGCAG CTGCA^G SalI 205 GTCGAC G^TCGAC SmlI 553 CTCAAG C^T[CT][GA]AG StyI 200 CCTTGG C^C[AT][AT]GG Tru9I 733 TTAA T^TAA Van91I 685 CCATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 57 GGATCC [GA]^GATC[CT] XmnI 31 GAATTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccI 205 GTCGAC GT^[AC][GT]AC 831 GTATAC AflIII 476 ACACGT A^C[GA][CT]GT 887 ACATGT BalI 107 TGGCCA TGG^CCA 371 TGGCCA BfaI 541 CTAG C^TAG 917 CTAG BpmI 26 CTCCAG CTGGAG(16/14) 801 CTCCAG BsaJI 200 CCTTGG C^C[ACGT][ACGT]GG 836 CCGTGG BstEII 603 GGTGACC G^GT[ACGT]ACC 840 GGTCACC BstPI 603 GGTGACC G^GT[ACGT]ACC 840 GGTCACC EcoO65I 603 GGTGACC G^GT[ACGT]ACC 840 GGTCACC EcoRI 31 GAATTC G^AATTC 338 GAATTC HincII 145 GTCAAC GT[CT]^[GA]AC 205 GTCGAC HindII 145 GTCAAC GT[CT]^[GA]AC 205 GTCGAC MaeI 541 CTAG C^TAG 917 CTAG MluNI 107 TGGCCA TGG^CCA 371 TGGCCA MscI 107 TGGCCA TGG^CCA 371 TGGCCA MspA1I 493 CAGCTG C[AC]G^C[GT]G 689 CAGCTG MwoI 234 GCCCAAGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 429 GCTGCTTCTGC NlaIV 57 GGATCC GG[ACGT]^[ACGT]CC 122 GGAACC PvuII 493 CAGCTG CAG^CTG 689 CAGCTG SfcI 67 CTGCAG C^T[GA][CT]AG 490 CTACAG TaqI 206 TCGA T^CGA 363 TCGA TspRI 386 CACTG CA[GC]TG[ACGT][ACGT]^ 824 CAGTG TthHB8I 206 TCGA T^CGA 363 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI BbvI BseRI BsiYI BslI BspHI BssKI BstF5I BstNI BstXI DdeI DpnII EaeI EcoRII Fnu4HI FokI HaeIII HphI ItaI MaeIII MboI MnlI MslI MvaI NdeII NlaIII RcaI RsaI Sau3AI ScrFI SfaNI TseI Tsp45I Tsp509I