################################## # NO CUT ENZYMES ( 167 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiWI BsmAI BsmBI BsoBI BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HpaI HpaII KasI KpnI Ksp632I KspI MamI MfeI MluI MroI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI PstI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AciI 363 CCGC CCGC(-3/-1) AclI 511 AACGTT AA^CGTT AcsI 31 GAATTT [GA]^AATT[CT] AlwI 240 GATCC GGATC(4/5) AlwNI 475 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 31 GAATTT [GA]^AATT[CT] AspHI 453 GAGCAC G[AT]GC[AT]^C BalI 371 TGGCCA TGG^CCA BbsI 798 GTCTTC GAAGAC(2/6) BfaI 244 CTAG C^TAG BglII 299 AGATCT A^GATCT BmyI 453 GAGCAC G[AGT]GC[ACT]^C BpuAI 798 GTCTTC GAAGAC(2/6) BsiEI 360 CGACCG CG[GA][CT]^CG BsiHKAI 453 GAGCAC G[AT]GC[AT]^C BsmI 680 GAATGC GAATGC(1/-1) Bsp1286I 453 GAGCAC G[AGT]GC[ACT]^C BspMI 125 ACCTGC ACCTGC(4/8) BstEII 108 GGTCACC G^GT[ACGT]ACC BstPI 108 GGTCACC G^GT[ACGT]ACC BstXI 98 CCATGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG EaeI 371 TGGCCA [CT]^GGCC[GA] EcoO65I 108 GGTCACC G^GT[ACGT]ACC HindIII 896 AAGCTT A^AGCTT MaeI 244 CTAG C^TAG MaeII 512 ACGT A^CGT MluNI 371 TGGCCA TGG^CCA MscI 371 TGGCCA TGG^CCA MwoI 346 GCAGTCATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC Psp1406I 511 AACGTT AA^CGTT SexAI 104 ACCTGGT A^CC[AT]GGT SfaNI 463 GATGC GCATC(5/9) SfcI 867 CTACAG C^T[GA][CT]AG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### BbvI 492 GCAGC GCAGC(8/12) 287 GCTGC BsaJI 117 CCTGGG C^C[ACGT][ACGT]GG 467 CCCTGG Bsp1407I 773 TGTACA T^GTACA 830 TGTACA BsrGI 773 TGTACA T^GTACA 830 TGTACA BstYI 239 AGATCC [GA]^GATC[CT] 299 AGATCT DpnII 240 GATC ^GATC 300 GATC DraII 3 GGGACCC [GA]G^G[ACGT]CC[CT] 56 AGGACCC EcoO109I 3 GGGACCC [GA]G^G[ACGT]CC[CT] 56 AGGACCC Fnu4HI 287 GCTGC GC^[ACGT]GC 492 GCAGC HaeIII 354 GGCC GG^CC 372 GGCC ItaI 287 GCTGC GC^[ACGT]GC 492 GCAGC MboI 240 GATC ^GATC 300 GATC MflI 239 AGATCC [GA]^GATC[CT] 299 AGATCT MspA1I 20 CAGCTG C[AC]G^C[GT]G 493 CAGCTG NdeII 240 GATC ^GATC 300 GATC PpuMI 3 GGGACCC [GA]G^G[AT]CC[CT] 56 AGGACCC PvuII 20 CAGCTG CAG^CTG 493 CAGCTG Sau3AI 240 GATC ^GATC 300 GATC SspBI 773 TGTACA T^GTACA 830 TGTACA TfiI 264 GAATC G^A[AT]TC 432 GATTC TseI 287 GCTGC G^C[AT]GC 492 GCAGC XhoII 239 AGATCC [GA]^GATC[CT] 299 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AfaI AluI Asp700I AvaII BsiYI BslI BsmFI BspHI BssKI BstF5I BstNI Cac8I Cfr13I DdeI EcoRII FokI HinfI HphI MaeIII MboII MnlI MseI MslI MvaI NlaIII NlaIV PleI RcaI RsaI Sau96I ScrFI Tru9I Tsp45I Tsp509I TspRI XmnI