Restriction map for
pFN21AE5492
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# NO CUT ENZYMES ( 150 enzymes) #
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AatII Acc65I AccI AccII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AvaII
AviII BanI BanII BbeI BbrPI
BbvCI BclI BcnI BfrI BlpI
BmyI BpmI Bpu1102I BsaBI BsaHI
BseRI BsiEI BsiHKAI BsiWI BsmBI
BsoBI Bsp1286I BspDI BspLU11I BsrFI
BssHII BssSI Bst1107I BstBI BstUI
BstZ17I Bsu36I CelII CfoI Cfr10I
Cfr13I ClaI CpoI DraI DraII
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI Sau96I SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SspI StuI
SwaI TaqI Tth111I TthHB8I Van91I
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccIII 123 TCCGGA T^CCGGA
AcsI 94 AAATTC [GA]^AATT[CT]
AlwNI 10 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 94 AAATTC [GA]^AATT[CT]
AvrII 865 CCTAGG C^CTAGG
BalI 434 TGGCCA TGG^CCA
BamHI 120 GGATCC G^GATCC
BbsI 870 GAAGAC GAAGAC(2/6)
BcgI 5 CGATGCAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 983 GGATAC GTATCC(6/5)
BglI 479 GCCTCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 362 AGATCT A^GATCT
BlnI 865 CCTAGG C^CTAGG
BpuAI 870 GAAGAC GAAGAC(2/6)
BsaAI 339 CACGTA [CT]AC^GT[GA]
BsaI 788 GGTCTC GGTCTC(1/5)
BsaWI 123 TCCGGA [AT]^CCGG[AT]
BseAI 123 TCCGGA T^CCGGA
BsgI 567 CTGCAC GTGCAG(16/14)
BsmFI 108 GGGAC GGGAC(10/14)
Bsp1407I 836 TGTACA T^GTACA
BspEI 123 TCCGGA T^CCGGA
BspHI 467 TCATGA T^CATGA
BsrBI 586 CCGCTC CCGCTC(-3/-3)
BsrDI 412 GCAATG GCAATG(2/0)
BsrGI 836 TGTACA T^GTACA
BsrI 603 ACTGG ACTGG(1/-1)
BstAPI 9 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 171 GGTCACC G^GT[ACGT]ACC
BstPI 171 GGTCACC G^GT[ACGT]ACC
BstXI 161 CCATGTACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EaeI 434 TGGCCA [CT]^GGCC[GA]
EcoO65I 171 GGTCACC G^GT[ACGT]ACC
EcoT14I 865 CCTAGG C^C[AT][AT]GG
HapII 124 CCGG C^CGG
HincII 510 GTTGAC GT[CT]^[GA]AC
HindII 510 GTTGAC GT[CT]^[GA]AC
HpaII 124 CCGG C^CGG
MaeII 340 ACGT A^CGT
MluNI 434 TGGCCA TGG^CCA
MroI 123 TCCGGA T^CCGGA
MscI 434 TGGCCA TGG^CCA
MseI 280 TTAA T^TAA
MslI 231 CACCCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 10 CAGCTG C[AC]G^C[GT]G
MspI 124 CCGG C^CGG
PvuII 10 CAGCTG CAG^CTG
RcaI 467 TCATGA T^CATGA
SapI 588 GCTCTTC GCTCTTC(1/4)
SmlI 963 CTTGAG C^T[CT][GA]AG
SpeI 514 ACTAGT A^CTAGT
SspBI 836 TGTACA T^GTACA
StyI 865 CCTAGG C^C[AT][AT]GG
Tru9I 280 TTAA T^TAA
XbaI 1029 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 14 enzymes) #
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BsmAI 401 GTCTC GTCTC(1/5)
789 GTCTC
BsmI 525 GAATGC GAATGC(1/-1)
743 GAATGC
BspMI 188 ACCTGC ACCTGC(4/8)
823 GCAGGT
BstYI 120 GGATCC [GA]^GATC[CT]
362 AGATCT
EarI 589 CTCTTC CTCTTC(1/4)
992 CTCTTC
FauI 476 CCCGC CCCGC(4/6)
585 CCCGC
Ksp632I 589 CTCTTC CTCTTC(1/4)
992 CTCTTC
MflI 120 GGATCC [GA]^GATC[CT]
362 AGATCT
PleI 217 GACTC GAGTC(4/5)
859 GACTC
PstI 130 CTGCAG CTGCA^G
875 CTGCAG
Tsp45I 172 GTCAC ^GT[GC]AC
443 GTCAC
Tsp509I 95 AATT ^AATT
397 AATT
TspRI 878 CAGTG CA[GC]TG[ACGT][ACGT]^
887 CAGTG
XhoII 120 GGATCC [GA]^GATC[CT]
362 AGATCT
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# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
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AciI AfaI AluI AlwI BbvI
BfaI BsaJI BsiYI BslI BssKI
BstF5I BstNI Cac8I DdeI DpnII
EcoRII Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeI MaeIII MboI
MboII MnlI MvaI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI ScrFI
SfaNI SfcI TfiI TseI