(Linear) (Six-base) MAPSORT of: KIEE5492.seq Check: 2758 from: 1 to: 1035 KIEE5492.orf With 182 enzymes: SgfI * January 29, 2014 12:03 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 869 1035 Size: 869 166 AlwNI CAG_nnn'CTG Cuts at: 0 15 1035 Size: 15 1020 ApoI r'AATT_y Cuts at: 0 94 1035 Size: 94 941 AvrII C'CTAG_G Cuts at: 0 865 1035 Size: 865 170 BamHI G'GATC_C Cuts at: 0 120 1035 Size: 120 915 BbsI GAAGACnn'nnnn_ Cuts at: 0 877 1035 Size: 877 158 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 28 1035 Size: 28 1007 BciVI GTATCCnnnnn_n' Cuts at: 0 977 1035 Size: 977 58 BglI GCCn_nnn'nGGC Cuts at: 0 485 1035 Size: 485 550 BglII A'GATC_T Cuts at: 0 362 1035 Size: 362 673 Bpu10I CC'TnA_GC Cuts at: 0 608 1035 Size: 608 427 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 984 1035 Size: 984 51 BsaI GGTCTCn'nnnn_ Cuts at: 0 794 1035 Size: 794 241 BsaAI yAC'GTr Cuts at: 0 341 1035 Size: 341 694 BsaWI w'CCGG_w Cuts at: 0 123 1035 Size: 123 912 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 552 1035 Size: 552 483 BsmI GAATG_Cn' Cuts at: 0 531 749 1035 Size: 531 218 286 Fragments arranged by size: 531 286 218 BspEI T'CCGG_A Cuts at: 0 123 1035 Size: 123 912 BspHI T'CATG_A Cuts at: 0 467 1035 Size: 467 568 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 197 814 1035 Size: 197 617 221 Fragments arranged by size: 617 221 197 BsrBI CCG'CTC Cuts at: 0 588 1035 Size: 588 447 BsrDI GCAATG_nn' Cuts at: 0 419 1035 Size: 419 616 BsrGI T'GTAC_A Cuts at: 0 836 1035 Size: 836 199 BstAPI GCAn_nnn'nTGC Cuts at: 0 15 1035 Size: 15 1020 BstEII G'GTnAC_C Cuts at: 0 171 1035 Size: 171 864 BstXI CCAn_nnnn'nTGG Cuts at: 0 168 1035 Size: 168 867 BstYI r'GATC_y Cuts at: 0 120 362 1035 Size: 120 242 673 Fragments arranged by size: 673 242 120 BtsI GCAGTG_nn' Cuts at: 0 884 1035 Size: 884 151 EaeI y'GGCC_r Cuts at: 0 434 1035 Size: 434 601 EarI CTCTTCn'nnn_ Cuts at: 0 595 998 1035 Size: 595 403 37 Fragments arranged by size: 595 403 37 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 869 1035 Size: 869 166 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 38 70 105 137 446 478 975 1007 Size: 38 32 35 32 309 32 497 32 Cuts at: 1007 1035 Size: 28 Fragments arranged by size: 497 309 38 35 32 32 32 32 28 HincII GTy'rAC Cuts at: 0 512 1035 Size: 512 523 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 642 1035 Size: 642 393 MscI TGG'CCA Cuts at: 0 436 1035 Size: 436 599 MslI CAynn'nnrTG Cuts at: 0 235 1035 Size: 235 800 MspA1I CmG'CkG Cuts at: 0 12 1035 Size: 12 1023 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 886 918 1035 Size: 886 32 117 Fragments arranged by size: 886 117 32 PstI C_TGCA'G Cuts at: 0 134 879 1035 Size: 134 745 156 Fragments arranged by size: 745 156 134 PvuII CAG'CTG Cuts at: 0 12 1035 Size: 12 1023 SapI GCTCTTCn'nnn_ Cuts at: 0 595 1035 Size: 595 440 SfcI C'TryA_G Cuts at: 0 130 875 930 1035 Size: 130 745 55 105 Fragments arranged by size: 745 130 105 55 SmlI C'TyrA_G Cuts at: 0 963 1035 Size: 963 72 SpeI A'CTAG_T Cuts at: 0 514 1035 Size: 514 521 StyI C'CwwG_G Cuts at: 0 865 1035 Size: 865 170 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 373 1035 Size: 373 662 TatI w'GTAC_w Cuts at: 0 164 239 836 1035 Size: 164 75 597 199 Fragments arranged by size: 597 199 164 75 XbaI T'CTAG_A Cuts at: 0 1029 1035 Size: 1029 6 Enzymes that do cut: AcuI AlwNI ApoI AvrII BamHI BbsI BcgI BciVI BglI BglII Bpu10I BpuEI BsaI BsaAI BsaWI BsgI BsmI BspEI BspHI BspMI BsrBI BsrDI BsrGI BstAPI BstEII BstXI BstYI BtsI EaeI EarI Eco57MI Hin4I HincII MmeI MscI MslI MspA1I PsrI PstI PvuII SapI SfcI SmlI SpeI StyI TaqII TatI XbaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI BaeI BanI BanII BbeI BbvCI BclI BfrBI BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BsaBI BsaHI BsaXI BseRI BseYI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BsrFI BssHII BssSI BstBI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI SspI StuI SwaI TstI Tth111I XcmI XhoI XmaI XmnI ZraI