################################## # NO CUT ENZYMES ( 168 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AflIII AgeI AlwNI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspI AvaI AviII BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BcnI BfrI BglI BglII BlpI Bpu1102I BsaAI BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DrdI EagI EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FauI FseI FspI HaeII HapII HgaI Hin1I HincII HindII HindIII HpaI HpaII KasI KpnI Ksp632I KspI MaeII MfeI MflI MluI MroI MseI MspA1I MspI MunI MvnI MwoI NaeI NarI NciI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tru9I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AhdI 734 GACCATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AluI 684 AGCT AG^CT Alw44I 469 GTGCAC G^TGCAC AlwI 318 GATCC GGATC(4/5) ApaLI 469 GTGCAC G^TGCAC AspEI 734 GACCATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 523 CCTAGG C^CTAGG BalI 757 TGGCCA TGG^CCA BbvI 266 GCTGC GCAGC(8/12) BclI 809 TGATCA T^GATCA BlnI 523 CCTAGG C^CTAGG BpmI 46 CTCCAG CTGGAG(16/14) BsaBI 312 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 25 GGTCTC GGTCTC(1/5) Bsp1407I 544 TGTACA T^GTACA BsrDI 576 CATTGC GCAATG(2/0) BsrGI 544 TGTACA T^GTACA BsrI 453 CCAGT ACTGG(1/-1) BssKI 755 CCTGG ^CC[ACGT]GG BstNI 755 CCTGG CC^[AT]GG CfoI 243 GCGC GCG^C DraIII 597 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 329 CCATGG C^C[GA][CT]GG EaeI 757 TGGCCA [CT]^GGCC[GA] Eam1105I 734 GACCATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 10 GAATTC G^AATTC EcoRII 755 CCTGG ^CC[AT]GG FbaI 809 TGATCA T^GATCA Fnu4HI 266 GCTGC GC^[ACGT]GC HhaI 243 GCGC GCG^C HinP1I 243 GCGC G^CGC ItaI 266 GCTGC GC^[ACGT]GC MamI 312 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 757 TGGCCA TGG^CCA MscI 757 TGGCCA TGG^CCA MvaI 755 CCTGG CC^[AT]GG NcoI 329 CCATGG C^CATGG NheI 165 GCTAGC G^CTAGC NlaIV 487 GGTCCC GG[ACGT]^[ACGT]CC NspI 397 GCATGT [GA]CATG^[CT] ScrFI 755 CCTGG CC^[ACGT]GG SfcI 555 CTACAG C^T[GA][CT]AG SspBI 544 TGTACA T^GTACA TaqI 500 TCGA T^CGA TseI 266 GCTGC G^C[AT]GC TthHB8I 500 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AcsI 10 GAATTC [GA]^AATT[CT] 784 AAATTC AfaI 156 GTAC GT^AC 545 GTAC ApoI 10 GAATTC [GA]^AATT[CT] 784 AAATTC AspHI 469 GTGCAC G[AT]GC[AT]^C 592 GTGCTC AvaII 487 GGTCC G^G[AT]CC 733 GGACC BbsI 861 GAAGAC GAAGAC(2/6) 198 GTCTTC BmyI 469 GTGCAC G[AGT]GC[ACT]^C 592 GTGCTC BpuAI 861 GAAGAC GAAGAC(2/6) 198 GTCTTC BsaJI 329 CCATGG C^C[ACGT][ACGT]GG 523 CCTAGG BsiHKAI 469 GTGCAC G[AT]GC[AT]^C 592 GTGCTC BsiYI 478 CCCTTCTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 519 CCTTCCTAGGG BslI 478 CCCTTCTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 519 CCTTCCTAGGG BsmAI 26 GTCTC GTCTC(1/5) 192 GTCTC BsmFI 42 GGGAC GGGAC(10/14) 488 GTCCC Bsp1286I 469 GTGCAC G[AGT]GC[ACT]^C 592 GTGCTC Cac8I 165 GCTAGC GC[ACGT]^[ACGT]GC 239 GCCAGC Cfr13I 487 GGTCC G^G[ACGT]CC 733 GGACC EcoT14I 329 CCATGG C^C[AT][AT]GG 523 CCTAGG HaeIII 459 GGCC GG^CC 758 GGCC PleI 44 GACTC GAGTC(4/5) 133 GACTC RsaI 156 GTAC GT^AC 545 GTAC Sau96I 487 GGTCC G^G[ACGT]CC 733 GGACC SfaNI 892 GATGC GCATC(5/9) 657 GCATC StyI 329 CCATGG C^C[AT][AT]GG 523 CCTAGG TfiI 34 GATTC G^A[AT]TC 89 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 19 enzymes) # ######################################### BfaI BstF5I DdeI DpnII FokI HinfI HphI MaeI MaeIII MboI MboII MnlI MslI NdeII NlaIII Sau3AI Tsp45I Tsp509I TspRI