################################## # NO CUT ENZYMES ( 39 enzymes) # ################################## AgeI AhdI Alw44I ApaLI AscI AseI AsnI Asp700I AspEI BsiWI BsrBI BssHII Bst1107I BstZ17I CpoI Eam1105I EcoRV EcoT22I FseI MluI NotI NruI NsiI PacI PaeR7I PinAI PmeI PshBI PvuI RsrII SapI SbfI SfiI SnaBI SpeI Sse8387I SwaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccIII 7457 TCCGGA T^CCGGA AclI 1426 AACGTT AA^CGTT AflII 336 CTTAAG C^TTAAG AocI 3279 CCTGAGG CC^T[ACGT]AGG Aor51HI 619 AGCGCT AGC^GCT ApaI 1405 GGGCCC GGGCC^C AvrII 8511 CCTAGG C^CTAGG BamHI 747 GGATCC G^GATCC BbrPI 7621 CACGTG CAC^GTG BbsI 6910 GAAGAC GAAGAC(2/6) BciVI 6608 GTATCC GTATCC(6/5) BfrI 336 CTTAAG C^TTAAG BglII 708 AGATCT A^GATCT BlnI 8511 CCTAGG C^CTAGG BpuAI 6910 GAAGAC GAAGAC(2/6) BsaBI 4995 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 7457 TCCGGA T^CCGGA BspDI 7210 ATCGAT AT^CGAT BspEI 7457 TCCGGA T^CCGGA BspHI 713 TCATGA T^CATGA Bsu36I 3279 CCTGAGG CC^T[ACGT]AGG ClaI 7210 ATCGAT AT^CGAT DrdI 8836 GACGGCAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 619 AGCGCT AGC^GCT Eco81I 3279 CCTGAGG CC^T[ACGT]AGG HpaI 8596 GTTAAC GTT^AAC MamI 4995 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 7457 TCCGGA T^CCGGA PmaCI 7621 CACGTG CAC^GTG PmlI 7621 CACGTG CAC^GTG Psp1406I 1426 AACGTT AA^CGTT RcaI 713 TCATGA T^CATGA ScaI 1697 AGTACT AGT^ACT SgrAI 2292 CACCGGCG C[GA]^CCGG[CT]G SphI 1818 GCATGC GCATG^C SspI 3884 AATATT AAT^ATT XbaI 5490 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AviII 2280 TGCGCA TGC^GCA 9089 TGCGCA BbeI 1643 GGCGCC GGCGC^C 2635 GGCGCC BcgI 1956 GCAGATTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 9170 GCAGAGCGTTCG BsaAI 3493 TACGTG [CT]AC^GT[GA] 7621 CACGTG BssSI 3142 CTCGTG CACGAG(-5/-1) 5299 CTCGTG BstBI 8759 TTCGAA TT^CGAA 9178 TTCGAA DraI 5028 TTTAAA TTT^AAA 6802 TTTAAA EagI 5828 CGGCCG C^GGCCG 8442 CGGCCG EclXI 5828 CGGCCG C^GGCCG 8442 CGGCCG Eco52I 5828 CGGCCG C^GGCCG 8442 CGGCCG FspI 2280 TGCGCA TGC^GCA 9089 TGCGCA KasI 1643 GGCGCC G^GCGCC 2635 GGCGCC KspI 1670 CCGCGG CCGC^GG 9108 CCGCGG MfeI 3132 CAATTG C^AATTG 7792 CAATTG MunI 3132 CAATTG C^AATTG 7792 CAATTG NaeI 50 GCCGGC GCC^GGC 2843 GCCGGC NarI 1643 GGCGCC GG^CGCC 2635 GGCGCC NgoMIV 50 GCCGGC G^CCGGC 2843 GCCGGC NheI 7200 GCTAGC G^CTAGC 9126 GCTAGC NspV 8759 TTCGAA TT^CGAA 9178 TTCGAA PshAI 1347 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 4429 GACGCCTGTC SacI 5422 GAGCTC GAGCT^C 6660 GAGCTC SacII 1670 CCGCGG CCGC^GG 9108 CCGCGG SfoI 1643 GGCGCC GGC^GCC 2635 GGCGCC SfuI 8759 TTCGAA TT^CGAA 9178 TTCGAA ######################################### # 3 OR MORE CUT ENZYMES ( 157 enzymes) # ######################################### AatII Acc65I AccI AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI ApoI Asp718I AspHI AspI AvaI AvaII BalI BanI BanII BbvCI BbvI BclI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspLU11I BspMI BsrDI BsrFI BsrGI BsrI BssKI BstAPI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeI NdeII NlaIII NlaIV NspI PflFI PflMI PleI PpuMI PstI PvuII RsaI SalI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmaI SmlI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoII XmaCI XmaI