Restriction map for
pFN21AE1139
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# NO CUT ENZYMES ( 144 enzymes) #
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AatII Acc65I AccII AclI AfaI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII BalI BanI BbeI
BbrPI BbvCI BcgI BclI BfrI
BglI BglII BlpI Bpu1102I BsaAI
BsiWI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstPI
BstUI BstXI Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
FbaI FseI FspI HaeII HhaI
HinP1I HincII HindII HindIII HpaI
HphI KasI KpnI KspI MfeI
MluI MluNI MscI MspA1I MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 879 GTATAC GT^[AC][GT]AC
AccIII 695 TCCGGA T^CCGGA
AciI 1032 GCGG CCGC(-3/-1)
AcsI 214 AAATTT [GA]^AATT[CT]
AcyI 1077 GACGCC G[GA]^CG[CT]C
ApoI 214 AAATTT [GA]^AATT[CT]
AspHI 688 GAGCTC G[AT]GC[AT]^C
AvaI 1081 CCCGAG C^[CT]CG[GA]G
AvrII 345 CCTAGG C^CTAGG
BfaI 346 CTAG C^TAG
BlnI 345 CCTAGG C^CTAGG
BpmI 58 CTGGAG CTGGAG(16/14)
BsaBI 412 GATCTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1077 GACGCC G[GA]^CG[CT]C
BseAI 695 TCCGGA T^CCGGA
BsgI 598 CTGCAC GTGCAG(16/14)
BsiEI 483 CGACCG CG[GA][CT]^CG
BsiHKAI 688 GAGCTC G[AT]GC[AT]^C
BsmBI 834 GAGACG CGTCTC(1/5)
BsmFI 1130 GGGAC GGGAC(10/14)
BsoBI 1081 CCCGAG C^[CT]CG[GA]G
BspEI 695 TCCGGA T^CCGGA
BspMI 677 ACCTGC ACCTGC(4/8)
Bst1107I 879 GTATAC GTA^TAC
BstZ17I 879 GTATAC GTA^TAC
DraI 730 TTTAAA TTT^AAA
DraII 1130 GGGACCC [GA]G^G[ACGT]CC[CT]
EarI 831 GAAGAG CTCTTC(1/4)
EcoNI 678 CCTGCCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1130 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoT22I 383 ATGCAT ATGCA^T
FauI 1032 GCGGG CCCGC(4/6)
Hin1I 1077 GACGCC G[GA]^CG[CT]C
Ksp632I 831 GAAGAG CTCTTC(1/4)
MaeI 346 CTAG C^TAG
MamI 412 GATCTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 695 TCCGGA T^CCGGA
NcoI 200 CCATGG C^CATGG
NsiI 383 ATGCAT ATGCA^T
NspI 176 ACATGC [GA]CATG^[CT]
PleI 946 GACTC GAGTC(4/5)
PpuMI 1130 GGGACCC [GA]G^G[AT]CC[CT]
SacI 688 GAGCTC GAGCT^C
SfaNI 659 GCATC GCATC(5/9)
StuI 1007 AGGCCT AGG^CCT
TaqI 482 TCGA T^CGA
Tsp45I 29 GTGAC ^GT[GC]AC
TthHB8I 482 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AvaII 225 GGACC G^G[AT]CC
1131 GGACC
BamHI 154 GGATCC G^GATCC
239 GGATCC
BbsI 283 GAAGAC GAAGAC(2/6)
742 GAAGAC
BciVI 549 GGATAC GTATCC(6/5)
929 GTATCC
BcnI 463 CCCGG CC^[GC]GG
933 CCGGG
BpuAI 283 GAAGAC GAAGAC(2/6)
742 GAAGAC
BsaI 674 GAGACC GGTCTC(1/5)
1170 GGTCTC
BsrDI 288 CATTGC GCAATG(2/0)
642 CATTGC
DdeI 925 CTGAG C^T[ACGT]AG
1068 CTCAG
EcoT14I 200 CCATGG C^C[AT][AT]GG
345 CCTAGG
HaeIII 471 GGCC GG^CC
1008 GGCC
MaeII 20 ACGT A^CGT
560 ACGT
MaeIII 29 GTGAC ^GT[ACGT]AC
557 GTTAC
MslI 576 CATCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
642 CATTGCCATG
NciI 463 CCCGG CC^[GC]GG
933 CCGGG
StyI 200 CCATGG C^C[AT][AT]GG
345 CCTAGG
Tsp509I 141 AATT ^AATT
215 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AluI AlwI AlwNI BanII BbvI
BmyI BsaJI BsaWI BseRI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DpnII DsaI EcoRII Fnu4HI FokI
HapII HgaI HinfI HpaII ItaI
MboI MboII MflI MnlI MseI
MspI MvaI MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI TfiI
Tru9I TseI TspRI XhoII