(Circular) (Six-base) MAPSORT of: pFN21AE1139.seq Check: 868 from: 1 to: 5896 pFN21AE1139 5896 bp With 182 enzymes: SgfI * August 16, 2010 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3706 278 Size: 53 83 186 936 2170 2468 Fragments arranged by size: 2468 2170 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2885 3239 1937 Size: 948 354 4594 Fragments arranged by size: 4594 948 354 Acc65I G'GTAC_C Cuts at: 3217 3217 Size: 5896 AclI AA'CG_TT Cuts at: 1563 4023 4396 1563 Size: 2460 373 3063 Fragments arranged by size: 3063 2460 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2135 2294 3959 5343 5611 1632 Size: 503 159 1665 1384 268 1917 Fragments arranged by size: 1917 1665 1384 503 268 159 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5699 AflIII A'CryG_T Cuts at: 1203 3193 4795 5772 1203 Size: 1990 1602 977 1327 Fragments arranged by size: 1990 1602 1327 977 AgeI A'CCGG_T Cuts at: 4727 4727 Size: 5896 AhdI GACnn_n'nnGTC Cuts at: 4625 4625 Size: 5896 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5896 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5896 AlwNI CAG_nnn'CTG Cuts at: 2092 2113 2449 3639 5211 2092 Size: 21 336 1190 1572 2777 Fragments arranged by size: 2777 1572 1190 336 21 ApaLI G'TGCA_C Cuts at: 3953 5109 3953 Size: 1156 4740 ApoI r'AATT_y Cuts at: 1493 2219 3205 3431 3583 5692 1493 Size: 726 986 226 152 2109 1697 Fragments arranged by size: 2109 1697 986 726 226 152 AseI AT'TA_AT Cuts at: 160 1032 4450 160 Size: 872 3418 1606 Fragments arranged by size: 3418 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5896 AvaI C'yCGr_G Cuts at: 1870 1943 3086 3221 1870 Size: 73 1143 135 4545 Fragments arranged by size: 4545 1143 135 73 AvrII C'CTAG_G Cuts at: 2350 2350 Size: 5896 BamHI G'GATC_C Cuts at: 2159 2244 3226 3593 2159 Size: 85 982 367 4462 Fragments arranged by size: 4462 982 367 85 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3217 4672 618 Size: 325 217 627 1430 1455 1842 Fragments arranged by size: 1842 1455 1430 627 325 217 BanII G_rGCy'C Cuts at: 1391 2325 2697 2904 3215 1391 Size: 934 372 207 311 4072 Fragments arranged by size: 4072 934 372 311 207 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2295 2754 928 Size: 971 396 459 4070 Fragments arranged by size: 4070 971 459 396 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4087 4121 1903 Size: 34 2150 34 3678 Fragments arranged by size: 3678 2150 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2548 2945 3788 4998 2548 Size: 397 843 1210 3446 Fragments arranged by size: 3446 1210 843 397 BclI T'GATC_A Cuts at: 1548 1548 Size: 5896 BfrBI ATG'CAT Cuts at: 2390 2390 Size: 5896 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4507 136 Size: 107 122 71 631 605 2835 1525 Fragments arranged by size: 2835 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5891 5891 Size: 5896 BlpI GC'TnA_GC Cuts at: 3313 3313 Size: 5896 Bme1580I G_kGCm'C Cuts at: 1790 3957 5113 1790 Size: 2167 1156 2573 Fragments arranged by size: 2573 2167 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5896 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2025 2293 3627 Size: 416 53 83 399 24 608 268 1334 Cuts at: 3627 4585 442 Size: 958 1753 Fragments arranged by size: 1753 1334 958 608 416 399 268 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5896 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2084 4556 1239 Size: 845 2472 2579 Fragments arranged by size: 2579 2472 845 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4021 4886 5184 4021 Size: 865 298 4733 Fragments arranged by size: 4733 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2673 3181 4559 882 Size: 617 1174 508 1378 2219 Fragments arranged by size: 2219 1378 1174 617 508 BsaAI yAC'GTr Cuts at: 493 1204 3687 493 Size: 711 2483 2702 Fragments arranged by size: 2702 2483 711 BsaBI GATnn'nnATC Cuts at: 2421 5890 2421 Size: 3469 2427 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3083 3703 4085 4720 Size: 53 83 186 936 1550 620 382 635 Cuts at: 4720 275 Size: 1451 Fragments arranged by size: 1550 1451 936 635 620 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2490 2700 3066 4329 4727 5001 5148 5559 2490 Size: 210 366 1263 398 274 147 411 2827 Fragments arranged by size: 2827 1263 411 398 366 274 210 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2555 2585 1178 Size: 30 165 30 1152 30 4489 Fragments arranged by size: 4489 1152 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2024 2063 2687 2705 3104 3113 3169 1186 Size: 838 39 624 18 399 9 56 3913 Fragments arranged by size: 3913 838 624 399 56 39 18 9 BseYI C'CCAG_C Cuts at: 2511 5099 5595 2511 Size: 2588 496 2812 Fragments arranged by size: 2812 2588 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2588 1163 Size: 573 852 4471 Fragments arranged by size: 4471 852 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2491 3346 4107 5135 1540 Size: 275 186 490 855 761 1028 2301 Fragments arranged by size: 2301 1028 855 761 490 275 186 BsiHKAI G_wGCw'C Cuts at: 2697 3957 4042 5113 2697 Size: 1260 85 1071 3480 Fragments arranged by size: 3480 1260 1071 85 BsmI GAATG_Cn' Cuts at: 3407 3500 3407 Size: 93 5803 BsmBI CGTCTCn'nnnn_ Cuts at: 2833 2833 Size: 5896 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2325 2697 2904 3215 3957 4042 5113 Size: 399 535 372 207 311 742 85 1071 Cuts at: 5113 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 535 399 372 311 207 85 BspEI T'CCGG_A Cuts at: 2700 5559 2700 Size: 2859 3037 BspHI T'CATG_A Cuts at: 3785 5515 3785 Size: 1730 4166 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2691 3251 844 Size: 1847 560 3489 Fragments arranged by size: 3489 1847 560 BsrBI CCG'CTC Cuts at: 1394 1823 3783 1394 Size: 429 1960 3507 Fragments arranged by size: 3507 1960 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2292 2646 4391 4565 65 Size: 1183 1044 354 1745 174 1396 Fragments arranged by size: 1745 1396 1183 1044 354 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4540 4727 1700 Size: 110 2730 187 2869 Fragments arranged by size: 2869 2730 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5896 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5896 BssSI C'ACGA_G Cuts at: 3956 4968 3956 Size: 1012 4884 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5896 BstBI TT'CG_AA Cuts at: 4700 4700 Size: 5896 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5896 BstYI r'GATC_y Cuts at: 1972 2159 2244 3069 3226 3593 3978 3995 5447 Size: 187 85 825 157 367 385 17 1452 Cuts at: 5447 5533 5545 5891 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 825 385 367 346 187 157 86 85 17 12 BstZ17I GTA'TAC Cuts at: 2886 2886 Size: 5896 BtgI C'CryG_G Cuts at: 513 1065 2004 2205 2550 2889 2905 513 Size: 552 939 201 345 339 16 3504 Fragments arranged by size: 3504 939 552 345 339 201 16 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5157 Fragments arranged by size: 5157 683 56 BtsI GCAGTG_nn' Cuts at: 3412 4206 4226 3412 Size: 794 20 5082 Fragments arranged by size: 5082 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5896 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5861 DraI TTT'AAA Cuts at: 2737 3200 2737 Size: 463 5433 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4903 809 Size: 507 3587 1802 Fragments arranged by size: 3587 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3343 4232 5861 Size: 54 1425 50 275 18 1513 889 1629 Cuts at: 5861 8 Size: 43 Fragments arranged by size: 1629 1513 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3343 1537 Size: 275 1531 4090 Fragments arranged by size: 4090 1531 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2831 3826 1353 Size: 1478 995 3423 Fragments arranged by size: 3423 1478 995 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4483 4857 5003 387 Size: 1064 138 2894 374 146 1280 Fragments arranged by size: 2894 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2695 2695 Size: 5896 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2084 2135 2294 3959 4556 5343 5611 Size: 393 452 51 159 1665 597 787 268 Cuts at: 5611 1239 Size: 1524 Fragments arranged by size: 1665 1524 787 597 452 393 268 159 51 EcoNI CCTnn'n_nnAGG Cuts at: 2687 2687 Size: 5896 EcoO109I rG'GnC_Cy Cuts at: 3136 3136 Size: 5896 EcoRI G'AATT_C Cuts at: 3205 3583 3205 Size: 378 5518 FspI TGC'GCA Cuts at: 4402 4402 Size: 5896 HaeII r_GCGC'y Cuts at: 1678 5043 1678 Size: 3365 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3061 3093 4537 4569 4611 Size: 32 135 32 1489 32 1444 32 42 Cuts at: 4611 4643 1373 Size: 32 2626 Fragments arranged by size: 2626 1489 1444 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3240 3486 669 Size: 988 281 1302 246 3079 Fragments arranged by size: 3079 1302 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5896 HpaI GTT'AAC Cuts at: 3486 3486 Size: 5896 KpnI G_GTAC'C Cuts at: 3221 3221 Size: 5896 MfeI C'AATT_G Cuts at: 3495 3495 Size: 5896 MluI A'CGCG_T Cuts at: 5772 5772 Size: 5896 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2098 3104 5010 5194 1305 Size: 793 1006 1906 184 2007 Fragments arranged by size: 2007 1906 1006 793 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4074 Fragments arranged by size: 4074 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2585 2651 3854 4213 4372 518 Size: 648 1419 66 1203 359 159 2042 Fragments arranged by size: 2042 1419 1203 648 359 159 66 MspA1I CmG'CkG Cuts at: 1606 3312 3989 5137 5382 1606 Size: 1706 677 1148 245 2120 Fragments arranged by size: 2120 1706 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2205 513 Size: 552 939 201 4204 Fragments arranged by size: 4204 939 552 201 NdeI CA'TA_TG Cuts at: 387 387 Size: 5896 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5896 NotI GC'GGCC_GC Cuts at: 3343 3343 Size: 5896 NsiI A_TGCA'T Cuts at: 2392 2392 Size: 5896 NspI r_CATG'y Cuts at: 1132 1229 2185 3254 4799 1132 Size: 97 956 1069 1545 2229 Fragments arranged by size: 2229 1545 1069 956 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5896 PciI A'CATG_T Cuts at: 4795 4795 Size: 5896 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5896 PfoI T'CCnGG_A Cuts at: 2937 2937 Size: 5896 PmeI GTTT'AAAC Cuts at: 3200 3200 Size: 5896 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3965 3997 5478 5510 3965 Size: 32 1481 32 4351 Fragments arranged by size: 4351 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3136 3136 Size: 5896 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5896 PsiI TTA'TAA Cuts at: 3466 3466 Size: 5896 PstI C_TGCA'G Cuts at: 830 3248 830 Size: 2418 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5896 SacI G_AGCT'C Cuts at: 2697 2697 Size: 5896 SalI G'TCGA_C Cuts at: 1936 3238 1936 Size: 1302 4594 SanDI GG'GwC_CC Cuts at: 3136 3136 Size: 5896 SbfI CC_TGCA'GG Cuts at: 3248 3248 Size: 5896 ScaI AGT'ACT Cuts at: 4144 5856 4144 Size: 1712 4184 SfcI C'TryA_G Cuts at: 826 1045 3244 4379 5060 5251 826 Size: 219 2199 1135 681 191 1471 Fragments arranged by size: 2199 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5896 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5896 SmaI CCC'GGG Cuts at: 1872 3223 1872 Size: 1351 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4000 4901 5163 820 Size: 197 926 2057 901 262 1553 Fragments arranged by size: 2057 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3687 493 Size: 3194 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5896 SphI G_CATG'C Cuts at: 1132 3254 1132 Size: 2122 3774 SspI AAT'ATT Cuts at: 5 52 3820 5 Size: 47 3768 2081 Fragments arranged by size: 3768 2081 47 StuI AGG'CCT Cuts at: 1517 3014 1517 Size: 1497 4399 StyI C'CwwG_G Cuts at: 513 1065 2004 2205 2350 3335 513 Size: 552 939 201 145 985 3074 Fragments arranged by size: 3074 985 939 552 201 145 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2805 3038 3923 3940 4093 4278 4723 Size: 1255 1225 233 885 17 153 185 445 Cuts at: 4723 325 Size: 1498 Fragments arranged by size: 1498 1255 1225 885 445 233 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4142 5854 96 Size: 274 80 33 51 1443 2165 1712 138 Fragments arranged by size: 2165 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5856 5888 5856 Size: 32 5864 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5896 XbaI T'CTAG_A Cuts at: 3232 3232 Size: 5896 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5896 XmaI C'CCGG_G Cuts at: 1870 3221 1870 Size: 1351 4545 XmnI GAAnn'nnTTC Cuts at: 4025 4025 Size: 5896 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3704 276 Size: 53 83 186 936 2170 2468 Fragments arranged by size: 2468 2170 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BplI Bpu10I BsiWI BstXI Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI