(Linear) (Six-base) MAPSORT of: KIEE5648.seq Check: 5899 from: 1 to: 1455 KIEE5648.orf With 182 enzymes: SgfI * December 5, 2014 13:04 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 32 1455 Size: 32 1423 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 417 725 801 1455 Size: 417 308 76 654 Fragments arranged by size: 654 417 308 76 AflIII A'CryG_T Cuts at: 0 938 1455 Size: 938 517 AlwNI CAG_nnn'CTG Cuts at: 0 26 830 1455 Size: 26 804 625 Fragments arranged by size: 804 625 26 AseI AT'TA_AT Cuts at: 0 1354 1455 Size: 1354 101 AvaI C'yCGr_G Cuts at: 0 148 178 418 1455 Size: 148 30 240 1037 Fragments arranged by size: 1037 240 148 30 AvrII C'CTAG_G Cuts at: 0 915 1455 Size: 915 540 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 925 958 1455 Size: 925 33 497 Fragments arranged by size: 925 497 33 BanI G'GyrC_C Cuts at: 0 44 281 562 1455 Size: 44 237 281 893 Fragments arranged by size: 893 281 237 44 BanII G_rGCy'C Cuts at: 0 149 1455 Size: 149 1306 BbsI GAAGACnn'nnnn_ Cuts at: 0 706 1455 Size: 706 749 BciVI GTATCCnnnnn_n' Cuts at: 0 953 1455 Size: 953 502 BfrBI ATG'CAT Cuts at: 0 1398 1455 Size: 1398 57 BglI GCCn_nnn'nGGC Cuts at: 0 22 290 1455 Size: 22 268 1165 Fragments arranged by size: 1165 268 22 BglII A'GATC_T Cuts at: 0 212 1455 Size: 212 1243 BlpI GC'TnA_GC Cuts at: 0 256 1455 Size: 256 1199 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 206 763 1455 Size: 206 557 692 Fragments arranged by size: 692 557 206 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 532 1455 Size: 532 923 BsaHI Gr'CG_yC Cuts at: 0 386 1455 Size: 386 1069 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 113 143 1455 Size: 113 30 1312 Fragments arranged by size: 1312 113 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 719 1455 Size: 719 736 BsiHKAI G_wGCw'C Cuts at: 0 149 167 1455 Size: 149 18 1288 Fragments arranged by size: 1288 149 18 Bsp1286I G_dGCh'C Cuts at: 0 149 167 1455 Size: 149 18 1288 Fragments arranged by size: 1288 149 18 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 32 360 1455 Size: 32 328 1095 Fragments arranged by size: 1095 328 32 BsrDI GCAATG_nn' Cuts at: 0 957 1455 Size: 957 498 BssSI C'ACGA_G Cuts at: 0 8 1455 Size: 8 1447 BstBI TT'CG_AA Cuts at: 0 1288 1455 Size: 1288 167 BstYI r'GATC_y Cuts at: 0 212 591 1455 Size: 212 379 864 Fragments arranged by size: 864 379 212 BtsI GCAGTG_nn' Cuts at: 0 831 1455 Size: 831 624 DrdI GACnn_nn'nnGTC Cuts at: 0 1193 1455 Size: 1193 262 EaeI y'GGCC_r Cuts at: 0 329 969 1455 Size: 329 640 486 Fragments arranged by size: 640 486 329 EarI CTCTTCn'nnn_ Cuts at: 0 275 1158 1318 1455 Size: 275 883 160 137 Fragments arranged by size: 883 275 160 137 EcoICRI GAG'CTC Cuts at: 0 147 1455 Size: 147 1308 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 206 417 725 763 801 1455 Size: 206 211 308 38 38 654 Fragments arranged by size: 654 308 211 206 38 38 EcoO109I rG'GnC_Cy Cuts at: 0 23 406 1020 1168 1455 Size: 23 383 614 148 287 Fragments arranged by size: 614 383 287 148 23 HaeII r_GCGC'y Cuts at: 0 205 1455 Size: 205 1250 HincII GTy'rAC Cuts at: 0 98 1455 Size: 98 1357 HindIII A'AGCT_T Cuts at: 0 707 1455 Size: 707 748 MslI CAynn'nnrTG Cuts at: 0 1381 1455 Size: 1381 74 MspA1I CmG'CkG Cuts at: 0 1065 1455 Size: 1065 390 NsiI A_TGCA'T Cuts at: 0 1400 1455 Size: 1400 55 NspI r_CATG'y Cuts at: 0 130 942 1038 1455 Size: 130 812 96 417 Fragments arranged by size: 812 417 130 96 PasI CC'CwG_GG Cuts at: 0 881 1455 Size: 881 574 PciI A'CATG_T Cuts at: 0 938 1455 Size: 938 517 PpuMI rG'GwC_Cy Cuts at: 0 1020 1455 Size: 1020 435 PspXI vC'TCGA_Gb Cuts at: 0 148 1455 Size: 148 1307 PstI C_TGCA'G Cuts at: 0 43 450 1455 Size: 43 407 1005 Fragments arranged by size: 1005 407 43 PvuII CAG'CTG Cuts at: 0 1065 1455 Size: 1065 390 SacI G_AGCT'C Cuts at: 0 149 1455 Size: 149 1306 SfcI C'TryA_G Cuts at: 0 39 446 1455 Size: 39 407 1009 Fragments arranged by size: 1009 407 39 SmaI CCC'GGG Cuts at: 0 180 420 1455 Size: 180 240 1035 Fragments arranged by size: 1035 240 180 SmlI C'TyrA_G Cuts at: 0 148 511 1455 Size: 148 363 944 Fragments arranged by size: 944 363 148 SphI G_CATG'C Cuts at: 0 1038 1455 Size: 1038 417 StyI C'CwwG_G Cuts at: 0 302 566 915 1455 Size: 302 264 349 540 Fragments arranged by size: 540 349 302 264 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 182 269 1043 1455 Size: 182 87 774 412 Fragments arranged by size: 774 412 182 87 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 113 145 1455 Size: 113 32 1310 Fragments arranged by size: 1310 113 32 XhoI C'TCGA_G Cuts at: 0 148 1455 Size: 148 1307 XmaI C'CCGG_G Cuts at: 0 178 418 1455 Size: 178 240 1037 Fragments arranged by size: 1037 240 178 Enzymes that do cut: AarI AcuI AflIII AlwNI AseI AvaI AvrII BaeI BanI BanII BbsI BciVI BfrBI BglI BglII BlpI BpmI BpuEI BsaHI BsaXI BseRI BsiHKAI Bsp1286I BspMI BsrDI BssSI BstBI BstYI BtsI DrdI EaeI EarI EcoICRI Eco57MI EcoO109I HaeII HincII HindIII MslI MspA1I NsiI NspI PasI PciI PpuMI PspXI PstI PvuII SacI SfcI SmaI SmlI SphI StyI TaqII TstI XhoI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI AsiSI BamHI BbeI BbvCI BcgI BclI Bme1580I BmgBI BmrI BmtI BplI Bpu10I BsaI BsaAI BsaBI BsaWI BseYI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII BstAPI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII EagI EciI EcoNI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HpaI KasI KpnI MfeI MluI MmeI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PacI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI StuI SwaI TatI Tth111I XbaI XcmI XmnI ZraI